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| 1 | Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds显示文摘Reporter genes produce a protein product in transfected cells that can be easily measured in intact or lysed cells and they have been extensively used in numerous basic and applied research applications.Over the past 10 years,reporter gene assays have been widely accepted and used for analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related dioxin-like compounds in various types of matrices,such as biological,environmental,food and feed samples,given that high-resolution instrumental analysis techniques are impractical for large-scale screening analysis.The most sensitive cell-based reporter gene bioassay systems developed are the mechanism-based CALUX(Chemically Activated Luciferase Expression) and CAFLUX(Chemically Activated Fluorescent Expression) bioassays,which utilize recombinant cell lines containing stably transfected dioxin(AhR) responsive firefly luciferase or enhanced green fluorescent protein(EGFP) reporter genes,respectively.While the current CALUX and CAFLUX bioassays are very sensitive,increasing their lower limit of sensitivity,magnitude of response and dynamic range for chemical detection would significantly increase their utility,particularly for those samples that contain low levels of dioxin-like HAHs(i.e.,serum) .In this study,we report that the addition of modulators of cell signaling pathways or modification of cell culture conditions results in significant improvement in the magnitude and overall responsiveness of the existing CALUX and CAFLUX cell bioassays. | BASTON David S. KHAN Elaine SORRENTINO Claudio DENISON Michael S. | 2010 | Science China Chemistry2010，53，5： | 2 |
| 2 | Steel fiber as shear reinforcement in beams显示文摘 | Baston GB Jenkins E Spatney R | 1972 | Journal of the American Concrete Institute Proceeding1972，69，10： | 1 |
| 3 | Multimer formation and ligand recognition by the long pentraxin PTX3: similarities and differences with the short pentraxins C-reactive protein and serum amyloid P eomponent显示文摘 | Bottazzi B Vouret-Craviari V Bastone A | 1997 | J Biol Chem1997，272，32： | 1 |
| 4 | Behavior of reinforced con- crete beams with closely spaced Reinforcement显示文摘 | ROMUALDI J P BASTON G B | 1963 | ACI Mate- rials Journal1963，60，6： | 1 |
| 5 | Long pentraxin PTX3 up-regulates tissue factor expression in human endothelial cells: a novel link between vascular inflammation and clotting activation 显示文摘 | Napoleone E Di Santo A Bastone A | 2002 | Arterioseler Thromb Vasc Biol2002，22，5： | 1 |
| 6 | Cyclohex-1-ene carboxylic acids:synthesis and biological evaluation of novel inhibitors of human 5α-reductase显示文摘 | Baston E Salem O Hartmann R W | 2003 | Arch Pharm Pharm Med Chem2003，1，： | 1 |
| 7 | The long pentraxin PTX3 as alink among innate immunity,inflammation,and female fertility显示文摘 | Bottazzi B Bastone A Doni A el al | 2006 | JLeukoc Biol2006，79，5： | 1 |
| 8 | An investigation of the radiochemical stability of ionic liquids显示文摘 | Allen D Baston G Bracley A | 2002 | Green Chemistry2002，4，2： | 1 |
| 9 | Pentraxins at the crossroads be- tween innate immunity, inflammation, matrix deposition, and female fertili-ty显示文摘 | Garlanda C Bottazzi B Bastone A | 2005 | Annual review of immunology2005，23，4： | 1 |
| 10 | Oral immunization of rabbits with VP60 particles confers protection against rabbit hemorrhalzic disease显示文摘 | Plana Duran J Bastons M Rodriguez M J | 1996 | Arch Virol1996，141，8： | 1 |
| 11 | Glutamate transporters in the spinal cord of the wobbler mouse显示文摘 | Bigini P Bastone A Mennini T | 2001 | Neuroreport2001，12，9： | 1 |
| 12 | Long pentraxin PTX3 upregulates tissue factor expression in human endothelial cells: a novel link between vascular inflammation and clotting active显示文摘 | Napoleone E Di Santo A Bastone A | 2002 | Arterioscler Thromb Vasc Biol2002，22，5： | 1 |
| 13 | Patterns of gene expression in the bovine corpus luteum following repeated intrauterine infusions of low doses of prostaglandin F2 alpha显示文摘 | Atli MO Bender RW Mehta V Bastons MR Luo W Vezina CM Wiltbank MC | | 0，，04： | 1 |
| 14 | Etfficacy of an inactivated vaccine for prevention of reproductive failure induced by porcine reproductive and respiratory syndrome virus显示文摘 | Planat-Duran J Bastons M Urniza A | 1997 | Vet Microbiol1997，55，14： | 1 |
| 15 | Long pentraxin PTX3 upregulate tissue factor expression in human endothelia cells:a novel link between vascular inflammation and clotting activation 显示文摘 | Napolene E Di Santo A Bastone A | 2002 | Arterioscler Thromb Yasc Biol2002，22，5： | 1 |
| 16 | Synthetic amyloid-beta oligomers impair long-term memo- ry independently of cellular priori protein 显示文摘 | Baldueei C Beeg M Stravalaci M Bastone A Selip A Biasini E | 2010 | Proc Natl Acad Sc/U S A2010，107，5： | 1 |
| 17 | Feeling in control during labour:concepts,correlates and consequences显示文摘 | Green JM Baston HA | 2003 | Birth-Iss Perinat C2003，4，30： | 1 |
| 18 | Delayed Neuroreal Death Prevented by Inhibition of Increased Hydroxyl Radical Formation in a Transient Cerebral Ischemia 显示文摘 | Yamamoto T Bastons JM Silva JA | 1997 | Brain Res1997，762，： | 1 |
| 19 | Pentraxins at the crossroads between innate immunity,inflammation,matrix deposition,and female fertility显示文摘 | Garlanda C Bottazzi B Bastone A | 2005 | Annu Rev Immunol2005，23，： | 1 |
| 20 | Epidemiology of dental trauma: a review of the literature 显示文摘 | Bastone EB Freer TJ McNamara JR | 2000 | Aust Dent J2000，45，1： | 1 |
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