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| 1 | Effects of retinoic acid on proliferation,phenotype and expression of cyclin-dependent kinase inhibitors in TGF-β1-stimulated rat hepatic stellate cells显示文摘AIM To study the molecular mechanisms ofretinoic acid(RA)on proliferation andexpression of cyclin-dependent kinase inhibitors(CKI),i.e.p16,p21 and p27 in cultured rathepatic stellate cells(HSC)stimulated withtransforming growth factor beta 1(TGF-β1).METHODS HSC were isolated from healthy ratlivers and cultured.After stimulated with1 mg/L TGF-β1,subcultured HSC were treatedwith or without 1 nmol/L RA.MTT assay,immunocytochemistry(ICC)for p16,p21,p27and α-smooth muscle actin(α-SMA)protein,insitu hybridization(ISH)for retinoic acidreceptor beta 2(RAR-β2)and p16,p21 and p27mRNA and quantitative image analysis(partially)were performed.RESULTS RA inhibited HSC proliferation(41.50%,P<0.05),decreased the protein levelof α-SMA(55.09%,P<0.05),and induced HSCto express RAR-β2 mRNA.In addition,RAincreased the protein level of p16(218.75%,P<0.05)and induced p21 protein expression;meanwhile,p27 was undetectable by ICC in bothcontrol and RA-treated HSC.However,RA hadno influence on the mRNA levels of p16,p21 orp27 as determined by ISH.CONCLISION Up-regulation of p16 and p21 on post-transcriptional level may contribule, in part to RA inhibition of TGF-β1-initiated rat HSC activation in vitro. | Guang Cun Huang Jin Sheng Zhang Yue E Zhang Department of Pathology School of Basic Medical Sciences,Fudan University.Shanghai 200032,China | 2000 | World Journal of Gastroenterology2000，6，6： | 23 |
| 2 | Down-Regulation of CD44 Contributes to the Differentiation of HL-60 Cells Induced by ATRA or HMBA显示文摘CD44 is highly expressed in human acute myeloid leukemia (AML) cells. Some experiments had shown that it was possible to reverse differentiation blockage in AML cells by CD44 ligation with specific antibodies, indicating that CD44 was closely related to the differentiation of leukemia cells. The differentiation of acute promyelocytic leukemia cell line HL-60 cells could be induced by all trans-retinoic acid (ATRA) and hexamethylene bisacetamide (HMBA), but so far the mechanism was not demonstrated clearly. In the present study, we investigated whether ATRA or HMBA induced the growth arrest of HL-60 cells by down-regulating the expression of CD44. The results indicated that the proliferation of HL-60 cells was obviously inhibited and the differentiation was induced by both ATRA and HMBA. The decreased expression of CD44 and cyclin E mRNA, and the increased expression of p27 and p21 at mRNA levels were observed. Furthermore, there was a negative correlation between the expression of CD44 and p27. It was concluded that ATRA and HMBA played a role in the differentiation induction of HL-60 cells, which was mediated by the down-regulation of CD44, accompanied by down-regulation of cyclin E, and up-regulation of p27 and p21 mRNA. | Jianing Liu1, Gaofeng Bi1, Pei’e Wen1, Weihua Yang1, Xia Ren1, Tianhua Tang1, Chao Xie1, Wei Dong1 and Guosheng Jiang1, 21Department of Hemato-oncology, Institute of Basic Medicine, Shandong Academy of Medical Sciences Key Laboratory for Modern Medicine and Technology of Shandong Province Key Medical Laboratory for Tumor Immunology & Traditional Chinese Medicine Immunology of Shandong Province, Jinan, Shandong 250062, China | 2007 | Cellular & Molecular Immunology2007，4，1： | 12 |
| 3 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Mad2004，27，2： | 1 |
| 4 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Med2004，27，2： | 1 |
| 5 | Soluble adhesion molecules in acute ischemic stroke 显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Med2004，27，2： | 1 |
| 6 | Soluble adhesion molecules in acute isehemie stroke显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Med2004，27，2： | 1 |
| 7 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Med2004，27，2： | 1 |
| 8 | Solids flux for rigid spheres 显示文摘 | Shannon P T Stroupe E Tm E M Batch and continuous thickening Basic theory | 1963 | Ind Eng Chem Fundam1963，2，3： | 1 |
| 9 | Ultrasound evaluation of uterine scar after Cesarean section and next birth 显示文摘 | Basic E Basic-Cetkovic V Kozaric H | 2012 | Med Arch2012，66，31： | 1 |
| 10 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic A Basic V Topic E | 2004 | Clin Invest Med2004，27，： | 1 |
| 11 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Med2004，27，2： | 1 |
| 12 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Med2004，27，： | 1 |
| 13 | Ultrasound evaluation of uterine scar after Cesarean section and next birth显示文摘 | Basic E Basic-Cetkovic V Kozaric H | 2012 | Med Arh2012，66，3： | 1 |
| 14 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | SIMUNDIC AM BASIC V TOPIC E | 2004 | Clin Invest Med2004，27，： | 1 |
| 15 | Conization as treatment of choice for precancerous changes and university cervical cancer at the Department of Obstetrics and Gynecology of Clinical Center of Sarajevo University in 2009显示文摘 | Basic E Kozaric H Kozaric M | | 0，，03： | 1 |
| 16 | Conization as treatment of choice for precancerous changes and university cervical cancer at the Department of Obstetrics and Gynecology of Clinical Center of Sarajevo University in 2009显示文摘 | Basic E Kozarie H Kozarie M etal | 2010 | Med Arh2010，64，3： | 1 |
| 17 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic AM Basic V Top ic E | 2004 | Clin InvestMed2004，27，： | 1 |
| 18 | Ultrasound evaluation of uterine sear after cesarean section显示文摘 | Basic E Basic-Cetkovic V Kozaric H | 2012 | Acta Inform Med2012，20，3： | 1 |
| 19 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Med2004，27，2： | 1 |
| 20 | Soluble adhesion molecules in acute ischemic stroke显示文摘 | Simundic AM Basic V Topic E | 2004 | Clin Invest Med2004，27，2： | 1 |
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