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| 1 | Hepatoprotective role of ganoderma lucidum polysaccharide against BCG-induced immune liver injury in mice显示文摘AIM: To examine the effect of ganoderma lucidumpolysaccharide (GLP) on the immune liver injuryinduced by BCG infection, and investigate therelationship between degrees of hepatic damage andNO production in mice.METHODS: Immune hepatic injury was markedlyinduced by BCG-pretreatment (125 mg.kg-1, 2-week, iv)or by BCG-pretreatment plus lipopolysaccharide (LPS,125 μg.kg-1, 12-hour, iv) in mice in vivo.Hepatocellulardamage induced by BCG-pretreated plus inflammatorycytokines mixture (CM), which was included TNF-α, IL1β, IFN-γ and LPS in culture medium in vitro.Administration of GLP was performed by oral orincubating with culture medium at immune stimulisimultaneity. Liver damage was determined by activityof alanine aminotransferase (ALT) in serum and inhepatocytes cultured supernatant, by liver weightchanges and histopathological examination. NOproduction in the cultured supematant was determinedby the Griess reaction. Moreover, inducible nitric oxidesynthase (iNOS) protein expression was alsoexaminated by immunohistochemi1cal method.RESULTS: Immune hepatic injury was markedly inducedby BCG or BCG plus inflammatory cytokines in BALB/cmice in vivoand in vitro. Under BCG-stimulated condition,augment of the liver weight and increase of the serum/supernatant ALT level were observed, as well asgranuloma forming and inflammatory cells soakage wereobserved by microscopic analysis within liver tissues.Moreover, NO production was also increased by BCG or/and CH stimuli in the culture supernatant, and a lot ofiNOS positive staining was observed in BCG-prestimulated hepatic sections. Application of GLPsignificantly mitigated hepatic tumefaction, decreasedALT enzyme release and NO production in serum/supernatant, improved the pathological changes ofchronic and acute inflammation induced by BCG-stimuliin mice. Moreover, the immunohistochemical resultshowed that GLP inhibited iNOS protein expression inBCG-immune hepatic damage model.CONCLUSION: The present study indicates that NOparticipates in immune liver injury induced byMycobacterium bovis BCG infection. The mechanismsof protective roles by GLP for BCG-induced immune liverinjury may be due to influence NO production in mice. | Guo-Liang Zhang Ye-Hong Wang Wei Ni Hui-Ling Teng Zhi-Bin Lin Department of Pharmacology,School of Basic Medical Sciences,Beijing University,Beijing 100083,China | 2002 | World Journal of Gastroenterology2002，8，4： | 47 |
| 2 | Prevention and Treatment of Osteoporosis with Chinese Herbal Medicines显示文摘In this paper,laboratory reports,medication rules,formulating principles,and research methods are summarized and analyzed,including single herb,compound herbs,and the problems in the prevention and treatment of osteoporosis(OP) within the past decade.With widely recognized efficacy,satisfying achievements have been achieved in preventing and treating OP with Chinese herbal medicines(CHM).However,due to the complex constituents,the pharmacological activities and mechanism of CHM are not clear yet,and there is no unified standard on the diagnosis and syndrome differentiation of OP and the efficacy evaluation of CHM in the treatment.Accordingly,the research in the future should focus on the pharmacology and standardization of CHM in treating OP. | ZHAO Xiao-ling1,2,FENG Yu-xiong1,2,3,PENG Yong1,2 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China 2.Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicines,Ministry of Education,Beijing 100193,China 3.College of Basic Medicine,Guangxi University of Chinese Medicine,Nanning 530001,China | 2012 | Chinese Herbal Medicines2012，4，4： | 28 |
| 3 | Effects of retinoic acid on proliferation,phenotype and expression of cyclin-dependent kinase inhibitors in TGF-β1-stimulated rat hepatic stellate cells显示文摘AIM To study the molecular mechanisms ofretinoic acid(RA)on proliferation andexpression of cyclin-dependent kinase inhibitors(CKI),i.e.p16,p21 and p27 in cultured rathepatic stellate cells(HSC)stimulated withtransforming growth factor beta 1(TGF-β1).METHODS HSC were isolated from healthy ratlivers and cultured.After stimulated with1 mg/L TGF-β1,subcultured HSC were treatedwith or without 1 nmol/L RA.MTT assay,immunocytochemistry(ICC)for p16,p21,p27and α-smooth muscle actin(α-SMA)protein,insitu hybridization(ISH)for retinoic acidreceptor beta 2(RAR-β2)and p16,p21 and p27mRNA and quantitative image analysis(partially)were performed.RESULTS RA inhibited HSC proliferation(41.50%,P<0.05),decreased the protein levelof α-SMA(55.09%,P<0.05),and induced HSCto express RAR-β2 mRNA.In addition,RAincreased the protein level of p16(218.75%,P<0.05)and induced p21 protein expression;meanwhile,p27 was undetectable by ICC in bothcontrol and RA-treated HSC.However,RA hadno influence on the mRNA levels of p16,p21 orp27 as determined by ISH.CONCLISION Up-regulation of p16 and p21 on post-transcriptional level may contribule, in part to RA inhibition of TGF-β1-initiated rat HSC activation in vitro. | Guang Cun Huang Jin Sheng Zhang Yue E Zhang Department of Pathology School of Basic Medical Sciences,Fudan University.Shanghai 200032,China | 2000 | World Journal of Gastroenterology2000，6，6： | 23 |
| 4 | Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes显示文摘AIM To study the effects of aminoguanidine(AG) and two L-arginine analogues Nω-nitro-L-arginine methyl ester (L-NAME) and Nω-nitro-L-arginine (L-NNA) on nitric oxide (NO) productioninduced by cytokines (TNF-α, IL-11β, and IFN-γ)and bacterial lipopolysaccharide (LPS) mixture(CM) in the cultured rat hepatocytes, andexamine their mechanisms action.METHODS Rat hepatocytes were incubatedwith AG, L-NAME, L-NNA, Actinomycin D (ActD)and dexamethasene in a medium containing CM(LPS plus TNF-α, IL-1β, and IFN-γ) for 24 h. NOproduction in the cultured supernatant wasmeasured with the Griese reaction. IntracellularcGMP level was detected with radioimmunoasey.RESULTS NO production was markedlyblocked by AG and L-NAME in a dose-dependentmanner under inflammatory stimuli conditiontriggered by CM in vitro. The rate of themaximum inhibitory effects of L-NAME (38.9%)was less potent than that obtained with AG(53.7%, P<0.05). There was no significantdifference between the inhibitory effects of AGand two L-arginine analogues on intracellularcGMP accumulation in rat cultured hepatocytes.Non-specific NOS expression inhibitordexamethasone ( DEX ) and iNOS mRNAtranscriptional inhibitor ActD also significantlyinhibited CM-induced NO production. AG(0.1mmol.L-1) and ActD (0.2ng@Lt) wereequipotent in decreasing NO production inducedby inflammatory stimuli in vitro, and botheffects were more potent than that induced bynon-selectivity NOS activity inhibitor L-NAME(0. 1 mmol@ L- 1) under similar stimuli conditions(P | Guo Liang Zhang Ye Hong Wang Hui Ling Teng Zhi Bin Lin Department of Pharmacology,School of Basic Medical Sciences,Beijing University,Beijiog 100083,ChinaDr.Guo Liang Zhang graduated from Xinxiang Medical College in 1982,got Ph.D.at Nagoya City University Medical School,Japan in 1994,finished postdoctoral research at Beijing Medical Univcrsity in 1996,now an associate professor of pharmacology,specialized in hepatic pharmacology,having 15 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 20 |
| 5 | The IAP family: endogenous caspase inhibitors with multiple biological activities显示文摘IAPs (inhibitors of apoptosis) are a family of proteins containing one or more characteristic BIR domains. These proteins have multiple biological activities that include binding and inhibiting caspases, regulating cell cycle progression, and modulating receptor-mediated signal transduction. Our recent studies found the IAP family members XIAP and c-IAP1 are ubiquitinated and degraded in proteasomes in response to apoptotic stimuli in T cells, and their degradation appears to be important for T cells to commit to death. In addition to three BIR domains, each of these IAPs also contains a RING finger domain.We found this region confers ubiquitin protease ligase (E3) activity to IAPs, and is responsible for the auto-ubiquitination and degradation of IAPs after an apoptotic stimulus. Given the factthat IAPs can bind a variety of proteins, such as caspases and TRAFs, it will be of interest to characterize potential substrates of the E3 activity of IAPs and the effects of ubiquitination byIAPs on signal transduction, cell cycle, and apoptosis. | YANG YI LI, XIAO MING LI(Laboratory of Immune Cell Biology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA) | 2000 | Cell Research2000，10，3： | 19 |
| 6 | Effects of hypoxia,hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 in hepatic stellate cells显示文摘AIM To study the effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 (MMP-2) in hepatic stellate cells ( HSC).``METHODS The expressions of MMP-2, tissue inhibitor of mjatrix metalloproteinese-2 ( TIMP-2 ) and membrane type matrix metalloproteiness-l (MT1-MMP) in cultured rat HSC were detected by immunocytochemistry (ICC) and in situ hybridization (ISH). The contents of MMP-2 and TIMP-2 in culture supernatant were detected with ELISA and the activity of MMP-2 in supematant was revealed by zymography.``RESULTS In the situation of hypoxia for 12 h, the expression of MMP-2 protein wss enhenced (hypoxiagroup positive indexes: 5.7 ± 2.0, n = 10; control: 3.2 ±1 .0. n -- 7; P<0.05). while TIMP-2 protein wss decreased in HSC ( hypoxia group positive indexes: 2.5 ± 0.7, n =10: control: 3.6 ± 1.0, n = 7; P | Ping-Sheng Chen~(1,2) Wei-Rong Zhai~1 Xiao-Mei Zhou~3 Jin-Sheng Zhang~1 Yue-E Zhang~1 Yu-Qin Ling~1 Ying-Hong Gu~1 1 Department of Pathology,School of Basic Medical Sciences,Fudan University,Shanghai 200032,China2 Ping-Sheng Chen now works in the Department of Pathology,School of Basic Medical Sciences the (former Nanjing Railway Medical College),Southeast University,Nanjing 210009,China3 Institute of Cancer Research,Shanghai 200032,China | 2001 | World Journal of Gastroenterology2001，7，5： | 18 |
| 7 | The cloning of 3'-truncated preS/S gene from HBV genomic DNA and its expression in transgenic mice显示文摘INTRODUCTIONHepatitis B virus (HBV) is regarded as one of themain etiologic factors involved in the developmentof human hepatocellular carcinoma (HCC).The open reading frame (orf)of X gene of HBVencoded a transactivating factor is the evidence thatstrongly supported the notion that the X gene ofHBV DNA integrated in HCC genomic DNA couldcontribute to the carcinogenesis of liver cells byactivation of some related cellular genes | Yi Ping Hu~1 Yu Cheng Yao~1 Jian Xiu Li~1 Xin Min Wang~1 Hong Li~2 Zhong Hua Wang~1 Zhang Heng Lei~3 1 Department of Cell Biology,Second Military Medical University,Shanghai 200433,China 2 Department of Biology,Department of Basic Medicine,West-China University of Medical Sciences,Chengdu 610041,China 3 Department of Biology,North Sichuan Medical College,Nanchong 637007,China | 2000 | World Journal of Gastroenterology2000，6，5： | 18 |
| 8 | Experimental research on TECA-I bioartificial liver support system to treat canines with acute liver failure显示文摘AIM To evaluate the efficacy and safety of the TECA-lbioartificial liver support system (BALSS) in treating canines with acute liver failure (ALF).``METHODS Ten canines with ALF induced by 80% liver resection received BALSS treatment (BALSS group).Blood was perfused through a hollow fiber tube containing 1 1010 porcine hepatocytes. Four canines with ALF were treated with BALSS without porcine hepatocytes (control group), and five canines with ALF received drug treatment (drug group). Each treatment lasted 6 hours.``RESULTS BALSS treatment yielded beneficial effects for partial liver resection-induced ALF canines with survival and decreased plasma ammonia, ALT, AST and BIL. There was an obvious decrease in PT level and increase in PA level, and there were no changes in the count of lymphocytes, immunoglobulins (IgA, IgG and IgM) and complement (C3 and C4) levels after BALSS treatment. In contrast, for the canines with ALF in non-hepatocyte BALSS group (control group) and drug group, there were no significant changes in ammonia, ALT, AST, BIL, PT and PA levels. ALF canines in BALSS group, control group and drug group lived respectively an average time of 108.0 h _- 12.0 h, 24.0 h -+ 6.0 h and 20.4 h + 6.4 h, and three canines with ALF survived in BALSS group.``CONCLUSION TECA-I BALSS is efficacious and safe for ALF canines induced by parcial liver resection. | Xiao-Ping Chen~2 Yi-Long Xue~1 Xin-Jian Li~1 Zuo-Yun Zhang~1 Yan-Ling Li~1 Zhi-Qiang Huang~2 1 Institute of Basic Medical Sciences,2 Department of Hepatobiliary Surgery,PLA General Hospital,Beijing 100853,China | 2001 | World Journal of Gastroenterology2001，7，5： | 17 |
| 9 | Lie symmetries of mechanical systems with unilateral holonomic constraints显示文摘In the recent twenty years, the study on the Lie symmetries and conserved quantities of constrained mechanical systems has been making great progress[1-5]. Up to now, however, all the studies are limited to the mechanical systems with ideal bilateral constraints. This note further studies the Lie symmetries of mechanical systems with unilateral holonomic constraints, and two problems of Lie symmetries for the systems are put forward and solved, i.e. finding the conserved quantity from a Lie symmetric transformation of the system and finding the corresponding Liesymmetry from an integral of the systemi ? 梛 ? Jsymmetry from an integral of the system. | ZHANG Yi & MEI Fengxiang1. Department of Basic Courses, Suzhou Institute of Urban Construction & Environmental Protection, Suzhou 215011, China 2. Department of Applied Mechanics, Beijing Institute of Technology, Beijing 10008I, China | 2000 | Chinese Science Bulletin2000，45，15： | 15 |
| 10 | 国家重点实验室在国家科技创新体系中的地位和作用显示文摘一、国家实验室对国家科技发展的作用
1.国家重点实验室对我国基础研究的发展发挥了不可替代的作用
改革开放以后,我国政府和科技界意识到基础研究的极端重要性和我国基础研究相对薄弱的状况,原国家计委决定集中一部分公共资源,有选择地在一些重要学科建设科学研究基地,称为国家重点实验室. | Department of Basic Research,MOST,Beijing 100862 | 2003 | 中国基础科学2003，，5： | 15 |
| 11 | Roux-en-Y gastric bypass promotes expression of PDX-1 and regeneration of β-cells in Goto-Kakizaki rats显示文摘AIM:To study the effects of Roux-en-Y gastric bypass(RYGB) on the expression of pancreatic duodenal homeobox-1(PDX-1) and pancreatic β-cell regeneration/neogenesis,and their possible mechanisms in diabetics.METHODS:Three groups of randomly selected nonobese diabetic Goto-Kakizaki(GK) rats were subjected to RYGB,sham-RYGB and sham-operation(sham-op) surgery,respectively.The rats were euthanized at postoperative 1,2,4 and 12 wk.Their pancreases were resected and analyzed using reverse transcription polymerase chain reaction to detect the mRNA of PDX-1.Anti-PDX-1 immunohistochemical(IHC) staining and Western blotting were used to detect the protein of PDX-1.Double IHC staining of anti-Brdu and-insulin was performed to detect regenerated β-cells.The index of double Brdu and insulin positive cells was calculated.RESULTS:In comparison with sham-RYGB and sham-op groups,a significant increase in the expressions of PDX-1 mRNA in RYGB group was observed at all experimental time points(1 wk:0.378 ± 0.013 vs 0.120 ± 0.010,0.100 ± 0.010,F = 727.717,P < 0.001;2 wk:0.318 ± 0.013 vs 0.110 ± 0.010,0.143 ± 0.015,F = 301.509,P < 0.001;4 wk:0.172 ± 0.011 vs 0.107 ± 0.012,0.090 ± 0.010,F = 64.297,P < 0.001;12 wk:0.140 ± 0.007 vs 0.120 ± 0.010,0.097 ± 0.015,F = 16.392,P < 0.001);PDX-1 protein in RYGB group was also increased significantly(1 wk:0.61 ± 0.01 vs 0.21 ± 0.01,0.15 ± 0.01,F = 3031.127,P < 0.001;2 wk:0.55 ± 0.00 vs 0.15 ± 0.01,0.17 ± 0.01,F = 3426.455,P < 0.001;4 wk:0.39 ± 0.01 vs 0.18 ± 0.01,0.22 ± 0.01,F = 882.909,P < 0.001;12 wk:0.41 ± 0.01 vs 0.20 ± 0.01,0.18 ± 0.01,F = 515.833,P < 0.001).PDX-1 mRNA and PDX-1 protein production showed no statistical significance between the two sham groups.Many PDX-1 positive cells could be found in the pancreatic islets of the rats in RYGB group at all time points.In addition,the percentage of Brdu-insulin double staining positive cells was higher in RYGB group than in the other two groups(1 wk:0.22 ± 0.13 vs 0.03 ± 0.06,0.03 ± 0.06,P < 0.05;2 wk:0.28 ± 0.08 vs 0.00 ± 0.00,0.03 ± 0.06,P < 0.05;4 wk:0.24 ± 0.11 vs 0.07 ± 0.06,0.00 ± 0.00,P < 0.001;12 wk:0.20 ± 0.07 vs 0.03 ± 0.06,0.00 ± 0.00,P < 0.05).CONCLUSION:RYGB can increase the expression of pancreatic PDX-1 and induce the regeneration of β-cells in GK rats.The associated regeneration of islet cells may be a possible mechanism that how RYGB could improve type 2 diabetes mellitus. | Zhen Li,Dong-Fei Li,Jing-Xing Dai,Yu Bai,Lin Yuan,Southern Medical University,Institute of Basic Medical Anatomy National Key Disciplines,Guangzhou 510515,Guangdong Province,China Hong-Ya Zhang,Lu-Xian Lv,Wen-Qiang Li,Second Affiliated Hospital,Xinxiang Medical College,Henan Province Key Laboratory of Biological Psychiatry,Xinxiang 453002,Henan Province,China Ou Sha,Department of Anatomy,Faculty of Medicine,Shenzhen University,Shenzhen 518060,Guangdong Province,China | 2010 | World Journal of Gastroenterology2010，16，18： | 14 |
| 12 | Inhibition of hepatitis B virus by oxymatrine in vivo显示文摘AIM To investigate the anti-HBV effect ofoxymatrine (oxy) in vivo.METHODS HBV transgenic mice were producedby micro-injection of a 4.2kb fragmentcontaining the complete HBV genomes.Expression level of HBsAg and HBcAg in thetransgenic mice liver was determined byimmunohistochemical assay.RESULTS Four groups (6 mice in each group)were injected intraperitoneally with oxy at thedosage of 100,200, and 300 mg/kg or with salineonce a day for 30 days. Both HBsAg and HBcAgwere positive in livers of all the six mice in thecontrol group (injected with saline), and werepositive in livers of two mice in 100 mg/kg groupand 300mg/kg group. In 200mg/kg group,HBsAg and HBcAg were negative in livers of allthe six mice. Based on the results, 200 mg/kg isthe ideal dosage to explore the effect of oxy atdifferent time points. According to the oxytreatment time, mice were divided into fourgroups: 10 d, 20 d, 30 d and 60 d (4 mice in eachgroup). Each mouse underwent liver biopsy twoweeks before the treatment of oxy. Down-regulation of HBsAg and HBcAg appeared aftertreatment of oxymatrine for 10 d and 20 d, Dane-like particles disappeared after the treatment ofoxy for 20d under electron microscopy,however, the expression level of HBsAg andHBcAg returned to normal 60 d later after oxytreatment.CONCLUSION oxymatrine can reduce thecontents of HBsAg and HBcAg in transgenic miceliver, longer treatment time and larger dosagedo not yield better effects. | Xiao Song Chen1 Guo Jun Wang1 Xiong Cai1 Hong Yu Yu2 Yi Ping Hu3 1Department of Infectious Diseases, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China2Department of Pathology, 3Department of Cell Biology, Department of Basic Medicine, the Second Military Medical University, Shanghai 200433, China | 2001 | World Journal of Gastroenterology2001，7，1： | 13 |
| 13 | 自然资源(基本)法立法可行性研究报告显示文摘本文立足于我国基本国情,全面分析了自然资源(基本)法立法的客观依据和现实条件,对自然资源(基本)法及替代方案进行了比较研究,在此基础上。 | Research Group of Feasibility Research of the Legislation of Natural Resources Basic law | 1998 | 兰州大学学报(社会科学版)1998，26，3： | 13 |
| 14 | Progress in miRNA target prediction and identification显示文摘Recently, the identification of miRNA targets has received much attention. The strategies to determine miRNA targets include bioinformatic prediction and experimental assays. The bioinformatic prediction methods are mainly based on the confirmed rules of interaction between miRNAs and their targets, and are carried out by programs, such as miRanda, TargetScan, TargetScanS, RNAhybrid, DIANA-microT, PicTar, RNA22 and FindTar, which follow well-known principles. The experimental assays to find miRNA targets employ immunoprecipitation of AGO proteins to identify interacting mRNAs, or the analysis of mRNA or protein levels to identify genes which can be regulated by miRNAs. The improvement of current bioinformatic and experimental assays and the development of novel assays will enable greater efficiency in the identification of miRNA targets and thus facilitate miRNA research. This paper describes progress in the prediction and identification of miRNA targets. | XIA Wei, CAO GuoJun & SHAO NingSheng· Beijing Institute of Basic Medical Sciences, Beijing 100850 | 2009 | Science China(Life Sciences)2009，52，12： | 13 |
| 15 | Robust adaptive fuzzy backstepping output feedback tracking control for nonlinear system with dynamic uncertainties显示文摘In this paper, an adaptive fuzzy output feedback control approach based on backstepping design is proposed for a class of SISO strict feedback nonlinear systems with unmeasured states, nonlinear uncertainties, unmodeled dynamics, and dynamical disturbances. Fuzzy logic systems are employed to approximate the nonlinear uncertainties, and an adaptive fuzzy state observer is designed for the states estimation. By combining backstepping technique with the fuzzy adaptive control approach, a stable adaptive fuzzy output feedback robust control is constructed recursively, in which nonlinear damping terms and a dynamical signal are introduced to counteract the nonlinear uncertainties and dominate the dynamic disturbances, respectively. It is proved that the proposed adaptive fuzzy control approach guarantees that all the signals of the closed-loop system are semi-globally uniformly bounded, and the tracking error converges to a small neighborhood of the origin. Simulation studies are included to illustrate the effectiveness of the proposed approach. | TONG ShaoCheng & LI YongMing Department of Basic Mathematics, Liaoning University of Technology, Jinzhou 121001, China | 2010 | Science China(Information Sciences)2010，53，2： | 13 |
| 16 | An unconstrained optimization method using nonmonotone second order Goldstein's line search显示文摘In this paper, an unconstrained optimization method using the nonmonotone second order Goldstein's line search is proposed. By using the negative curvature information from the Hessian,the sequence generated is shown to converge to a stationary point with the second order optimality conditions. Numerical tests on a set of standard test problems confirm the efficiency of our new method. | Wen-yu SUN~(1+) Qun-yan ZHOU~(1,2) ~1 School of Mathematics and Computer Science,Nanjing Normal University,Nanjing 210097,China ~2 Department of Basic Courses,Jiangsu Teachers University of Technology,Changzhou 213001,China | 2007 | Science China Mathematics2007，50，10： | 12 |
| 17 | Down-Regulation of CD44 Contributes to the Differentiation of HL-60 Cells Induced by ATRA or HMBA显示文摘CD44 is highly expressed in human acute myeloid leukemia (AML) cells. Some experiments had shown that it was possible to reverse differentiation blockage in AML cells by CD44 ligation with specific antibodies, indicating that CD44 was closely related to the differentiation of leukemia cells. The differentiation of acute promyelocytic leukemia cell line HL-60 cells could be induced by all trans-retinoic acid (ATRA) and hexamethylene bisacetamide (HMBA), but so far the mechanism was not demonstrated clearly. In the present study, we investigated whether ATRA or HMBA induced the growth arrest of HL-60 cells by down-regulating the expression of CD44. The results indicated that the proliferation of HL-60 cells was obviously inhibited and the differentiation was induced by both ATRA and HMBA. The decreased expression of CD44 and cyclin E mRNA, and the increased expression of p27 and p21 at mRNA levels were observed. Furthermore, there was a negative correlation between the expression of CD44 and p27. It was concluded that ATRA and HMBA played a role in the differentiation induction of HL-60 cells, which was mediated by the down-regulation of CD44, accompanied by down-regulation of cyclin E, and up-regulation of p27 and p21 mRNA. | Jianing Liu1, Gaofeng Bi1, Pei’e Wen1, Weihua Yang1, Xia Ren1, Tianhua Tang1, Chao Xie1, Wei Dong1 and Guosheng Jiang1, 21Department of Hemato-oncology, Institute of Basic Medicine, Shandong Academy of Medical Sciences Key Laboratory for Modern Medicine and Technology of Shandong Province Key Medical Laboratory for Tumor Immunology & Traditional Chinese Medicine Immunology of Shandong Province, Jinan, Shandong 250062, China | 2007 | Cellular & Molecular Immunology2007，4，1： | 12 |
| 18 | Cross-reactivity of hypervariable region 1 chimera of hepatitis C virus显示文摘AIM: To analyze the amino acid sequences of hypervariable region 1 (HVR1) of HCV isolates in China and to construct a combinatorial chimeric HVR1 protein having a very broad high cross-reactivity.
METHODS: All of the published HVR1 sequences from China were collected and processed with a computer program.Several representative HVR1's sequences were formulated based on a consensus profile and homology within certain subdivision. A few reported HVR1 mimotope sequences were also included for a broader representation. All of them were cloned and expressed in E.coli. The cross-reactivity of the purified recombinant HVR1 antigens was tested by ELISA with a panel of sera from HCV infected patients in China.Some of them were further ligated together to form a combinatorial HVR1 chimera.
RESULTS: Altogether 12 HVR1s were selected and expressed in E. coli and purified to homogeneity. All of these purified antigens showed some cross-reactivity with sera in a 27 HCV positive panel. Recombinant HVR1s of No. 1, 2, 4, and 8# showing broad cross-reactivities and complementarity with each other, were selected for the ligation elements. The chimera containing these 4 HVR1s was highly expressed in E. coli. The purified chimeric antigen could react not only with all the HCV antibody positive sera in the panel but also with 90/91 sera of HCV -infected patients.
CONCLUSION: The chimeric antigen was shown to have a broad cross-reactivity. It may be helpful for solving the problem caused by high variability of HCV, and in the efforts for a novel vaccine against the virus. | Bing-Shui Xiu Shi-Gan Ling Xiao-Guo Song He-Qiu Zhang Kun Chen Cui-Xia Zhu Laboratory of Molecular Virology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China | 2003 | World Journal of Gastroenterology2003，9，6： | 11 |
| 19 | Human papillomavirus DNA and P16~(INK4A) expression in concurrent esophageal and gastric cardia cancers显示文摘AIM:To investigate the relationship between human papillomavirus (HPV) infection and concurrent esophagus and gastric cardia cancer from the same patient (CC) and examine the significance of P16 INK4A protein expression.METHODS:Polymerase chain reaction was used to detect the presence of HPV type16 (HPV16).The expression of P16 INK4A protein was detected using immunohistochemistry.RESULTS:Among the CC specimens,HPV16-DNA was found in eight cases of esophageal squamous cell carcinoma (ESCC) and five cases of gastric cardia adenocarcinoma (GCA),respectively (47% vs 29%),and two of both ESCC and GCA.P16 INK4A was highly expressed in both ESCC and GCA.In the HPV-associated positive CC,higher P16 INK4A expression was observed in the GCA than in the ESCC (75% vs 25%,P < 0.05).CONCLUSION:HPV16 as a correlated risk factor may play an important role in the development of ESCC and GCA.P16 INK4A may be a screening index in the HPVassociated carcinoma of gastric cardia. | Guang-Cheng Ding,Tao Guo,Department of Gastroenterology,The First Affiliated Hospital and The Fifth Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,Henan Province,China Jing-Li Ren,Xin Song,Sheng-Li Zhou,Zong-Min Fan,LiDong Wang,Henan Key Laboratory for Esophageal Cancer Research,Department of Gastroenterology,The First Affiliated Hospital,College of Basic Medicine,Zhengzhou University,Zhengzhou 450052,Henan Province,China Fu-Bao Chang,Department of Thoracic Surgery,Linzhou Center Hospital,Linzhou 456500,Henan Province,China Ji-Lin Li,Department of Pathology,Yaocun Esophageal Cancer Hospital,Linzhou 456500,Henan Province,China Ling Yuan,Department of Oncoradiotherapy,Henan Province Tumor Hospital,Zhengzhou 450003,Henan Province,China Yi Zeng,Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China | 2010 | World Journal of Gastroenterology2010，16，46： | 11 |
| 20 | Influenza A virus H5N1 entry into host cells is through clathrin-dependent endocytosis显示文摘Influenza A virus H5N1 presents a major threat to human health. The entry of influenza virus into host cells is believed to be mediated by hemagglutinin (HA), a virus surface glycoprotein that can bind terminal sialic acid residues on host cell glycoproteins and glycolipids. In this study, we elucidated the pathways through which H5N1 enters human lung carcinoma cell line A549. We first proved that H5N1 can enter A549 cells via endocytosis, as lysosomotropic agents, such as bafilomycin A1 and chloroquine, can rescue H5N1-induced A549 cell death. By using specific inhibitors, and siRNAs that target the clathrin pathway, we further found that H5N1 could enter A549 cells via clathrin-mediated endocytosis, while inhibitors targeting caveolae-mediated endocytosis could not inhibit H5N1 cell entry. These findings expand our understanding of H5N1 pathogenesis and provide new information for anti-viral drug research. | WANG HongLiang & JIANG ChengYu National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China | 2009 | Science China(Life Sciences)2009，52，5： | 11 |
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