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| 1 | Dynamic expression of apoptosis-related genes during development of laboratory hepatocellular carcinoma and its relation to apoptosis显示文摘AIM:To explore the expression of p53,bcl-2,bax,survivin and the cell apoptosis during the development of tree shrew hepatocellular carcinoma(HCC),the relationship between expression of these genes,its impact on HCC development,and its relation to cell apoptosis.METHONS:Tree shrew HCC was induced with aflatoxin B1(AFB1),and regular biopsy of liver tissues was carried out and the biopsy tissues were collected during cancer inducement.Liver biopsy tisue and HCC tissue were collected from 35pre-cancerous experimental animats at wk 30 and 60 and at the 30th_,60th_,and 90th-wk,Liver biopsy tissues were collected from 13 blank control animals at wk 30,60,and 90.Expression of p53,bcl-2,bax,and survivin at each stage was examined by immunohistochemistry method.Apoptotic cells were detected in situ by the terminal deoxynucleotidyl transferase-mediated nick end labeling(tunel)technique.RESULTS:The apoptosis rate of normal hepatic cells was extremely low,whereas it increased during the formation of HCC.Expression of the apoptosis-related genes p53,bd-2,bax,and suvivin during the formation of HCC presented an increasing tendency.Expression of p53 did not noticeably relate to that of bcl-2,bax,and survivin,whereas expression of bcl-2 and bax was closely related.In HCC,p53 did not present a distinct relation to cell apoptosis,whereas its high level expression was probably related to liver cell proliferation.Survivin negetively correlated apoptosis index,and its overexpression could inhibit cell apoptosis.CONCLUSION:Apoptosis-related genes p53,bcl-2,bax,and survivin are all related to the occurrence of HCC.The anti-apoptosis effect of bcl-2 is influenced by bax,and ratio bcl/bax reflects more correctly the extent of cell apoptosis. | Xiao-Xian Duan Jing-Sheng Ou Yuan Li Jian-Jia Su Chao Ou Chun Yang Hui-Fen Yue Ke-Chen Ban | 2005 | World Journal of Gastroenterology2005，11，30： | 38 |
| 2 | Expression of IGF-Ⅱ,p53,p21 and HBxAg in precancerous events of hepatocarcinogenesis induced by AFBI and/or HBV in tree shrews显示文摘INTRODUCTIONIn order to study the relationship between oncogeneexpression and HCC generation,we observed theprecancerous hepatic GGT loci,IGF-Ⅱ,p53 andp21 expression during hepatocarcinogenesis of treeshrew induced by hepatitis B virus (HBV) and/oraflatoxin B1 (AFB1). | Qin LL Su JJ Li Y Yang C Ban KC Yian RQ | 2000 | World Journal of Gastroenterology2000，6，1： | 37 |
| 3 | Psychosocial determinants of irritable bowel syndrome显示文摘From a pure motor disorder of the bowel,in the past few years,irritable bowel syndrome (IBS) has become a multifactorial disease that implies visceral hypersensitivity,alterations at the level of nervous and humoral communications between the enteric nervous system and the central nervous system,alteration of the gut microflora,an increased intestinal permeability and minimum intestinal inflammation.Psychological and social factors can interfere with the communication between the central and enteric nervous systems,and there is proof that they are involved in the onset of IBS and influence the response to treatment and outcome.There is evidence that abuse history and stressful life events are involved in the onset of functional gastrointestinal disorders.In order to explain clustering of IBS in families,genetic factors and social learning mechanisms have been proposed.The psychological features,such as anxiety,depression as well as the comorbid psychiatric disorders,health beliefs and coping of patients with IBS are discussed in relation to the symptoms and outcome. | Teodora Surdea-Blaga Adriana Bban Dan L Dumitrascu | 2012 | World Journal of Gastroenterology2012，18，7： | 34 |
| 4 | Reaction mode between Si and Fe and evaluation of optimal species in poly-silicic-ferric coagulant显示文摘A kind of Fe-polysilicate polymer,poly-silicic-ferric(PSF)coagulant was prepared by co-polymerization(hydroxylation of mixtureof Fe3+and fresh polysilicic acid(PS)),in which PSF0.5,PSF1 or PSF3 denotes Si/Fe molar ratio of 0.5,1 or 3,respectively.Theeffects of Si/Fe ratio and reaction time(co-polymerization time or aging time)on the reaction mode between Si and Fe were studies,and the optimal species of PSF was evaluated by pH change during the preparation of PSF and coagulation tests.The results showedthat the characteristics of PSF are largely affected by both reaction time and Si/Fe ratio.PSF is found to be a essential complex of Si,Fe,and many other ions.The reaction mode between Si and Fe differs with various Si/Fe ratios.The pH of PSF0.5,PSF1 or PSF3tended to be stable when reaction time is 10,25 or 55 min,respectively,which is almost consistent with the time reaching the relativestable morphology that is just the optimal species of higher coagulation effciency.The optimal reaction time reaching optimal speciescan be evaluated by measuring the pH change during the polymerization process. | FU Ying YU Shui-li YU Yan-zhen QIU Li-ping HUI Ban | 2007 | Journal of Environmental Sciences2007，19，6： | 24 |
| 5 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 6 | Alteration of p53 and p21 during hepatocarcinogenesis in tree shrews显示文摘AIM: To investigate p53 mutation and p21 expression in hepatocarcinogenesis induced by hepatitis B virus (HBV) and aflatoxin B1 (AFB1) in tree shrews, and to reveal the role of these genes in hepatocarcinogenesis.METHODS: Tree shrews were divided into four groups:group A, those infected with HBV and fed with AFB1 (n = 39);group B, those infected with HBV alone (n = 28); group C,those fed with AFB1 alone (n = 29); and group D, normal controls (n = 20). The tree shrews underwent liver biopsies once every 15 wk. Expression of p53 and p21 proteins and genes in the biopsies and tumor tissues of the experimental tree shrews was detected, respectively, by immunohistochemistry,and by Southem blotting and reverse transcription-polymerase chain reaction and sequencing.RESULTS: The incidence of hepatocellular carcinomas (HCC) was higher in group A (66.7%) than that in group B (3.57%) and C (30%). The time of HCC occurrence was also earlier in group A than that in group C (120.0±16.6 wk vs 153.3±5.8 wk, respectively, P<0.01). p53 protein was not detected by immunohistochemistry in all groups before the 75^th wk of the experiment. At the 105^th wk, the positive rates fo p53 were 78.6%, 60% and 71.4% in groups A, B and C, respectively, which were significantly higher than that in group D (10%) (all P<0.05). An abnormal band of p53 gene was observed in groups A and C. The mutation points of p53gene in tree shrews with HCC were at codons 275, 78 and 13. The nucleotide sequence and amino acid sequence of tree shrew's wild-type p53 showed 91.7% and 93.4% homologies with those of human p53,respectively. The immunopositivity for p21 was found before HCC development. The incidence of HCC was significantly higher in tree shrews that were positive for p21 than those negative for p21 (80.0% vs 11.0%, P<0.001).The incidence of HCC in p21 positive animals in group A was significantly higher than those positive for p21 in group C (P | Jian-Jia Su Yuan Li Ke-Chen Ban Liu-Liang Qin Chun Yang Chao Ou Xiao-Xian Duan Hui-Yun Wang Rui-Qi Yang Young-Lk Lee | 2004 | World Journal of Gastroenterology2004，10，24： | 21 |
| 7 | KCNE3 R53H substitution in familial atrial fibrillation显示文摘 | ZHANG Dai-fu LIANG Bo LIN Jie LIU Ban ZHOU Qin-shu YANG Yi-qing | 2005 | Chinese Medical Journal2005，，20： | 18 |
| 8 | Effect of Dark Septate Endophytic Fungus Gaeumannomyces cylindrosporus on Plant Growth, Photosynthesis and Pb Tolerance of Maize (Zea mays L.)显示文摘Dark septate endophytic(DSE) fungi are ubiquitous and cosmopolitan, and occur widely in association with plants in heavy metal stress environment. However, little is known about the effect of inoculation with DSE fungi on the host plant under heavy metal stress.In this study, Gaeumannomyces cylindrosporus, which was isolated from Pb-Zn mine tailings in China and had been proven to have high Pb tolerance, was inoculated onto the roots of maize(Zea mays L.) seedlings to study the effect of DSE on plant growth, photosynthesis,and the translocation and accumulation of Pb in plant under stress of different Pb concentrations. The growth indicators(height, basal diameter, root length, and biomass) of maize were detected. Chlorophyll content, photosynthetic characteristics(net photosynthetic rate, transpiration rate, stomatal conductance, and intercellular CO_2 concentration), and chlorophyll fluorescence parameters in leaves of the inoculated and non-inoculated maize were also determined. Inoculation with G. cylindrosporus significantly increased height,basal diameter, root length, and biomass of maize seedlings under Pb stress. Colonization of G. cylindrosporus improved the efficiency of photosynthesis and altered the translocation and accumulation of Pb in the plants. Although inoculation with G. cylindrosporus increased Pb accumulation in host plants in comparison to non-inoculated plants, the translocation factor of Pb in plant body was significantly decreased. The results indicated that Pb was accumulated mainly in the root system of maize and the phytotoxicity of Pb to the aerial part of the plant was alleviated. The improvement of efficiency of photosynthesis and the decrease of translocation factor of Pb, caused by DSE fungal colonization, were efficient strategies to improve Pb tolerance of host plants. | BAN Yihui XU Zhouying YANG Yurong ZHANG Haihan CHEN Hui TANG Ming | 2017 | Pedosphere2017，27，2： | 16 |
| 9 | Genetic Variation and Yield Performance of Jerusalem Artichoke Germplasm Collected in China显示文摘Jerusalem artichoke (Helianthus tuberosus L.) is a perennial tuberous plant rich in inulin and is a potential energy crop.This study was conducted in a semiarid region of the Loess Plateau to investigate variations in morphological and agronomic characteristics and yield performance of 59 Jerusalem artichoke clones collected from 24 provinces in China.The germplasm we collected exhibited variations either in qualitative or in quantitative traits.Substantial genetic variations (genotype coefficient of variation >20%) in the main stem diameter,main stem number per plant,branch number per main stem,tuber number per plant,tuber size,tuber yield,and top yield (i.e.,aboveground biomass) were found as well.Seven clones had a tuber yield ranging between 9.1-10.6 t ha-1,7 clones had a top yield ranging between 18.1-31.3 t ha-1,and 15 clones had a total biomass yield (i.e.,tuber and top weight) between 25.0-35.0 t ha-1 on an oven-dried weight basis under the drought soil and climatic condition in the semiarid region.The 59 Jerusalem artichoke clones were clustered into 8 groups based on 17 quantitative traits,and indicated that the most prevalent clones planted by farmers exhibited relatively low variation.Correlation analysis was conducted on the morphology and agronomy of the clones.This investigation suggests possible genetic improvement of Jerusalem artichoke for a higher tuber yield or a top biomass yield,and as a promising application for inulin or bioenergy in semiarid regions. | LIU Zu-xin BAN Li-pu Yosef Steinberger XIE Guang-hui | 2011 | Agricultural Sciences in China2011，10，5： | 14 |
| 10 | Hepatitis B virus infection, diabetes mellitus, and their synergism for cholangiocarcinoma development: A casecontrol study in Korea显示文摘AIM: To identify possible risk factors and their synergism for cholangiocarcinoma development.METHODS: A hospital-based, case-control study in which we included 276 cholangiocarcinoma patients [193 extrahepatic cholangiocarcinoma(ECC) and 83 intrahepatic cholangiocarcinoma(ICC)], diagnosed at a training hospital in Korea between 2007 and 2013, and 552 healthy controls matched 2:1 for age, sex, and date of diagnosis. Risk factors for cholangiocarcinoma and possible synergism between those factors were evaluated using conditional logistic regression and synergism index, respectively.RESULTS:There was an association between cholangiocarcinoma and hepatitis B virus(HBV) infection, diabetes mellitus(DM), cholecystolithiasis, choledocholithiasis, and hepatolithiasis, with the adjusted odds ratios(AORs) of 4.1, 2.6, 1.7, 12.4, and 39.9, respectively. Synergistic interaction on the additive model was investigated between HBV infection and DM(AOR = 12.2; 95%CI: 1.9-80.1). In the subgroup analyses, cholecystolithiasis, choledocholithiasis, hepatolithiasis, and DM were significant risk factors for ECC(AOR = 2.0, 18.1, 14.9, and 2.0, respectively), whereas choledocholithiasis, hepatolithiasis, HBV infection, and DM were risk factors for ICC(AOR = 8.6, 157.4, 5.3 and 4.9, respectively). Synergistic interaction was also observed between HBV infection and DM(OR = 22.7; 95%CI: 2.4-214.1). However, there was no synergistic interaction between other significant risk factors for cholangiocarcinoma.CONCLUSION: In this Korean study, HBV infection and DM were found to exert independent and synergistic effects on the risk for cholangiocarcinoma, including ICC. Exploring the underlying mechanismsfor such synergy may lead to the development of cholangiocarcinoma prevention strategies in high-risk individuals. | Ban Seok Lee Eun-Cheol Park Seung Woo Park Chung Mo Nam Jaehoon Roh | 2015 | World Journal of Gastroenterology2015，21，2： | 13 |
| 11 | Chinese physicians' perceptions of fecal microbiota transplantation显示文摘AIM: To explore Chinese physicians' perceptions towards fecal microbiota transplantation(FMT) and to provide information and an assessment of FMT development in China.METHODS: A self-administered questionnaire was developed according to the FMT practice guidelines and was distributed to physicians in hospitals via Internet Research Electronic Data Capture(REDcap) software and electronic mails to assess their attitudes toward and knowledge of FMT. The questionnaire included a brief introduction of FMT that was followed by 20 questions. The participants were required to respond voluntarily, under the condition of anonymity and without compensation. Except for the fill-in-the-blank questions, all of the other questions were required in the REDcap data collection systems, and the emailed questionnaires were completed based on eligibility.RESULTS: Up to December 9, 2014, 844 eligible questionnaires were received out of the 980 distributed questionnaires, with a response rate of 86.1%. Among the participants, 87.3% were from tertiary hospitals, and there were 647(76.7%) gastroenterologists and 197(23.3%) physicians in other departments(nongastroenterologists). Gastroenterologists' awareness of FMT prior to the survey was much higher than non-gastroenterologists'(54.3 vs 16.5%, P < 0.001); however, acceptance of FMT was not statistically different(92.4 vs 87.1%, P = 0.1603). Major concerns of FMT included the following: acceptability to patients(79.2%), absence of guidelines(56.9%), and administration and ethics(46.5%). On the basis of understanding, the FMT indications preferred byphysicians were recurrent Clostridium difficile infection(86.7%), inflammatory bowel disease combined with Clostridium difficile infection(78.6%), refractory ulcerative colitis(70.9%), ulcerative colitis(65.4%), Crohn's disease(59.4%), chronic constipation(43.7%), irritable bowel syndrome(39.1%), obesity(28.1%) and type 2 diabetes(23.9%). For donor selection, the majority of physicians preferred individuals with a similar gut flora environment to the recipients. 76.6% of physicians chose lower gastrointestinal tract as the administration approach. 69.2% of physicians considered FMT a safe treatment. CONCLUSION: Chinese physicians have awareness and a high acceptance of FMT, especially gastroenterologists, which provides the grounds and conditions for the development of this novel treatment in China. Physicians' greatest concerns were patient acceptability and absence of guidelines. | Rong-Rong Ren Gang Sun Yun-Sheng Yang Li-Hua Peng Shu-Fang Wang Xiao-Hong Shi Jing-Quan Zhao Yong-Ling Ban Fei Pan Xue-Hong Wang Wei Lu Jian-Lin Ren Ying Song Jiang-Bin Wang Qi-Ming Lu Wen-Yuan Bai Xiao-Ping Wu Zi-Kai Wang Xiao-Mei Zhang Ye Chen | 2016 | World Journal of Gastroenterology2016，22，19： | 12 |
| 12 | Arbuscular Mycorrhizal Fungi in Wetland Habitats and Their Application in Constructed Wetland: A Review显示文摘Over the last three decades, the presence and functional roles of arbuscular mycorrhizal(AM) fungi in wetland habitats have received increasing attention. This review summarized the mycorrhizal status in wetlands and the effect of flooding on AM fungal colonization. Plants of 99 families living in 31 different habitats have been found to be associated with AM fungi, even including submerged aquatic plants and several plant species that were thought to be nonmycorrhizal(Cyperaceae, Chenopodiaceae, and Plumbaginaceae). The functions of AM fungi in wetland ecological systems could be concluded as their influences on the composition,succession, and diversity of the wetland plant community, and the growth and nutrition of wetland plants. Affecting the composition,succession, and diversity of the wetland plant community, AM fungi have positive, negative, or neutral effects on the performance of different wetland species under different conditions. The factors that affect the application effect of AM fungi in constructed wetland(CW) include flooding, phosphorus, plant species, aerenchyma, salinity, CW types, operation modes of CW, and wastewater quality.The generalist AM fungi strains can be established spontaneously, rapidly, and extensively in wastewater bioremediation technical installations; therefore, AM fungi can be considered ideal inhabitants of technical installations for the plant-based bioremediation of groundwater contaminated by organic pollutants or other contaminants. In the future, roles of AM fungi and factors that affect the purifying capacity of AM-CW system must be understood to optimize CW ecosystem. | XU Zhouying BAN Yihui JIANG Yinghe ZHANG Xiangling LIU Xiaoying | 2016 | Pedosphere2016，26，5： | 12 |
| 13 | Ursolic acid: An overview on its cytotoxic activities against breast and colorectal cancer cells显示文摘Ursolic acid(UA) is a pentacyclic triterpene of the ursane type. As a common chemical constituent among species of the family Lamiaceae, UA possesses a broad spectrum of pharmacological properties. This overview focuses on the anticancer properties of UA against breast cancer(BC) and colorectal cancer(CRC)that are most common among women and men, respectively. In vitro studies have shown that UA inhibited the growth of BC and CRC cell lines through various molecular targets and signaling pathways.There are several in vivo studies on the cytotoxic activity of UA against BC and CRC. UA also inhibits the growth of other types of cancer. Studies on structural modifications of UA have shown that the –OH groups at C3 and at C28 are critical factors influencing the cytotoxic activity of UA and its derivatives.Some needs for future research are suggested. Sources of information were from ScienceDirect, Google Scholar and PubMed. | Eric Wei Chiang Chan Chu Yong Soon Joash Ban Lee Tan Siu Kuin Wong Yew Woh Hui | 2019 | Journal of Integrative Medicine2019，17，3： | 11 |
| 14 | Genome-wide identification and characterization of HSP gene superfamily in whitefly (Bemisia tabacl)and expression profiling analysis under temperature stress显示文摘Heat shock proteins (HSP)are essential molecular chaperones that play important roles in the stress stimulation of insects.Bemisia tabaci,a phloem feeder and invasive species,can cause extensive crop damage through direct feeding and transmission of plant viruses.Here we employed comprehensive genomics approaches to identity HSP superfamily members in the Middle East Asia Minor 1 whitefly genome.In total,we identified 26 Hsp genes,including three Hsp90,17 Hsp70,one Hsp60 and five sHSP (small heat shock protein)genes.The HSP gene superfamily of whitefly is expanded compared with the other five insects surveyed here.The gene structures among the same families are relatively conserved.Meanwhile,the motif compositions and secondary structures of BtHsp proteins were predicted.In addition,quantitative polymerase chain reaction analysis showed that the expression patterns of BtHsp gene superfamily were diverse across different tissues of whiteflies.Most Hsp genes were induced or repressed by thermal stress (40℃)and cold treatment (4℃)in whitefly.Silencing the expression of BtHsp70-6 significantly decreased the survival rate of whitefly under 45℃.All the results showed the Hsps conferred thermo-tolerance or cold-tolerance to whiteflies that protect them from being affected by detrimental temperature conditions.Our observations highlighted the molecular evolutionary properties and the response mechanism to temperature assaults of Hsp genes in whitefly. | Xin-Ru Wang Chao Wang Fei-Xue Ban Dan-Tong Zhu Shu-Sheng Liu Xiao-Wei Wang | 2019 | Insect Science2019，26，1： | 11 |
| 15 | The construction of a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp.chinensis Makino)显示文摘Non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino) is one of the most important vegetables in eastern China.A genetic linkage map was constructed using 127 doubled haploid(DH) lines,and the DH population was derived from a commercial hybrid 'Hanxiao'(lines SW-13 × L-118).Out of the 614 polymorphic markers,43.49% were not assigned to any of the linkage groups(LGs).Chi-square tests showed that 42.67% markers were distorted from expected Mendelian segregation ratios,and the direction of distorted segregation was mainly toward the paternal parent L-118.After sequentially removing the markers that had an interval distance smaller than 1 cM from the upper marker,the overall quality of the linkage map was increased.Two hundred and sixty-eight molecular markers were mapped into 10 LGs,which were anchored to the corresponding chromosome of the B.rapa reference map based on common simple sequence repeat(SSR) markers.The map covers 973.38 cM of the genome and the average interval distance between markers was 3.63 cM.The number of markers on each LG ranged from 18(R08) to 64(R07),with an average interval distance within a single LG from 1.70 cM(R07) to 6.71 cM(R06).Among these mapped markers,169 were sequence-related amplified polymorphism(SRAP) mo-lecular markers,50 were SSR markers and 49 were random amplification polymorphic DNA(RAPD) markers.With further saturation to the LG,the current map offers a genetic tool for loci analysis for important agronomic traits. | Yan Cheng Jianfeng Geng Jingyi Zhang Qian Wang Qingyu Ban Xilin Hou | 2009 | Journal of Genetics and Genomics2009，36，8： | 9 |
| 16 | Clinical features and outcomes of 210 patients with idiopathic pulmonary fibrosis显示文摘 | Cai Miaotian Zhu Min Ban Chengjun Su Jin Ye Qiao Liu Yan Zhao Wen Wang Chen Dai Huaping | 2014 | Chinese Medical Journal2014，，10： | 8 |
| 17 | Incidence and Clinical Characteristics of Pulmonary Hypertension in Patients with Idiopathic Pulmonary Fibrosis显示文摘 | Wei Yan Li-Ying Peng Cheng-Jun Ban Xue-Feng Xu Min Zhu Yan Liu Shu Zhang Zhen-Guo Zhai Chen Wang Hua-Ping Dai | 2015 | Chinese Medical Journal2015，，7： | 8 |
| 18 | Quantitation of HBsAg predicts response to entecavir therapy in HBV genotype C patients显示文摘AIM:To analysis the factors that predict the response to entecavir therapy in chronic hepatitis patients with hepatitis B virus (HBV) genotype C. METHODS:Fifty patients [hepatitis B e antigen (HBeAg)-negative:HBeAg-positive = 26:24] with HBV genotype C, who received nave entecavir therapy for > 2 years, were analyzed. Patients who showed HBV DNA levels ≥ 3.0 log viral copies/mL after 2 years of entecavir therapy were designated as slow-responders, while those that showed < 3.0 log copies/mL were termed rapid- responders. Quantitative hepatitis B surface antigen (HBsAg) levels (qHBsAg) were determined by the Architect HBsAg QT immunoassay. Hepatitis B core-related antigen was detected by enzyme immunoassay. Pre-C and Core promoter mutations were determined using by polymerase chain reaction (PCR). Drug-resistance mutations were detected by the PCR-Invader method. RESULTS:At year 2, HBV DNA levels in all patients in the HBeAg-negative group were < 3.0 log copies/mL. In contrast, in the HBeAg-positive group, 41.7% were slow-responders, while 58.3% were rapid-responders. No entecavir-resistant mutants were detected in the slow-responders. When the pretreatment factors were compared between the slow-and rapid-responders; the median qHBsAg in the slow-responders was 4.57 log IU/mL, compared with 3.63 log IU/mL in the rapid-responders (P < 0.01). When the pretreatment factors predictive of HBV DNA-negative status at year 2 in all 50 patients were analyzed, HBeAg-negative status, low HBV DNA levels, and low qHBsAg levels were significant (P < 0.01). Multivariate analysis revealed that the low qHBsAg level was the most significant predictive factor (P = 0.03). CONCLUSION:Quantitation of HBsAg could be a useful indicator to predict response to entecavir therapy. | Etsuro Orito Kei Fujiwara Hiroshi Kanie Tesshin Ban Tomonori Yamada Katsumi Hayashi | 2012 | World Journal of Gastroenterology2012，18，39： | 8 |
| 19 | Risk factors of organ failure in cholangitis with bacteriobilia显示文摘AIM: To identify the risk factors for organ failure(OF) in cholangitis with bacteriobilia.METHODS: This study included 182 patients with acute cholangitis who underwent percutaneous transhepatic biliary drainage between January 2005 and April 2013. We conducted a retrospective analysis of comprehensive clinical and laboratory data.RESULTS: There were 24 cases(13.2%) of OF and five deaths(2.7%). Bile culture was positive for microbial growth in 130 out of 138(94.2%) patients. In multivariate analysis of 130 patients with positivebile cultures, significant predictive factors for OF were the presence of extended-spectrum beta-lactamase(ESBL) organisms in blood cultures, pre-existing renal dysfunction, and choledocholithiasis as an etiology, with odds ratios of 15.376, 6.319, and 3.573, respectively. We developed a scoring system with a regression coefficient of each significant variable. The OF score was calculated using the following equation:(2.7 × ESBL organisms in blood cultures) +(1.8 × pre-existing renal dysfunction) +(1.3 × choledocholithiasis). This scoring system for predicting OF was highly specific(99.1%) and had a positive predictive value of 86.2%.CONCLUSION: ESBL organisms in blood cultures, preexisting renal dysfunction, and choledocholithiasis are risk factors for OF in cholangitis with bacteriobilia. The OF scoring system may aid clinicians to identify a poor prognosis group. | Jae Min Lee Sang Hyub Lee Kwang Hyun Chung Jin Myung Park Ban Seok Lee Woo Hyun Paik Joo Kyung Park Ji Kon Ryu Yong-Tae Kim | 2015 | World Journal of Gastroenterology2015，21，24： | 8 |
| 20 | Pneumonia relevant to lung transplantation and pathogen distribution显示文摘 | HE Xuan DAI Hua-ping CHEN Qi-rui MIAO Jin-bai SUN Bing BAO Na HU Bin LI Hui WU An-shi BAN Cheng-jun GE Su-juan WANG Chen HOU Sheng-cai | 2013 | Chinese Medical Journal2013，，17： | 7 |
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