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| 1 | Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice显示文摘BACKGROUND Non-alcoholic fatty liver disease (NAFLD) has become an epidemic largely due to the worldwide increase in obesity. While lifestyle modifications and pharmacotherapies have been used to alleviate NAFLD, successful treatment options are limited. One of the main barriers to finding safe and effective drugs for long-term use in NAFLD is the fast initiation and progression of disease in the available preclinical models. Therefore, we are in need of preclinical models that (1) mimic the human manifestation of NAFLD and (2) have a longer progression time to allow for the design of superior treatments. AIM To characterize a model of prolonged high-fat diet (HFD) feeding for investigation of the long-term progression of NAFLD. METHODS In this study, we utilized prolonged HFD feeding to examine NAFLD features in C57BL/6 male mice. We fed mice with a HFD (60% fat, 20% protein, and 20% carbohydrate) for 80 wk to promote obesity (Old-HFD group, n = 18). A low-fat diet (LFD)(14% fat, 32% protein, and 54% carbohydrate) was administered for the same duration to age-matched mice (Old-LFD group, n = 15). An additional group of mice was maintained on the LFD (Young-LFD, n = 20) for a shorter duration (6 wk) to distinguish between age-dependent and age-independent effects. Liver, colon, adipose tissue, and feces were collected for histological and molecular assessments.RESULTS Prolonged HFD feeding led to obesity and insulin resistance. Histological analysis in the liver of HFD mice demonstrated steatosis, cell injury, portal and lobular inflammation and fibrosis. In addition, molecular analysis for markers of endoplasmic reticulum stress established that the liver tissue of HFD mice have increased phosphorylated Jnk and CHOP. Lastly, we evaluated the gut microbial composition of Old-LFD and Old-HFD. We observed that prolonged HFD feeding in mice increased the relative abundance of the Firmicutes phylum. At the genus level, we observed a significant increase in the abundance of Adercreutzia, Coprococcus, Dorea, and Ruminococcus and decreased relative abundance of Turicibacter and Anaeroplasma in HFD mice. CONCLUSION Overall, these data suggest that chronic HFD consumption in mice can mimic pathophysiological and some microbial events observed in NAFLD patients. | Kandy T Velázquez Reilly T Enos Jackie E Bader Alexander T Sougiannis Meredith S Carson Ioulia Chatzistamou James A Carson Prakash S Nagarkatti Mitzi Nagarkatti E Angela Murphy | 2019 | World Journal of Hepatology2019，11，8： | 6 |
| 2 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 3 | Fluvastatin inhibits hepatitis C replication in humans 显示文摘 | Bader T Fazili J Madhoun M | 2008 | Am J Gastroenterol2008，103，6： | 1 |
| 4 | A physical approach to protein structure prediction 显示文摘 | CRIVELLI S ESKOW E BADER B LAMBERTI V BYRD R SCHNABEL R HEAD-GORDON T | 2002 | Biophysical Journal2002，82，1： | 1 |
| 5 | Intracellular me chanics and mechanotransduction associated with chondrocyte deformation during pipette aspiration 显示文摘 | Ohashi T Hagiwara M Bader DL | 2006 | Biorheology2006，43，： | 1 |
| 6 | An analysis of the hydrogen bond in BASE - HF complexes using the theory of atoms in molecules 显示文摘 | Carroll M T Bader R F W | 1988 | Mol Phys1988，65，： | 1 |
| 7 | Liver tests are irrelevant when prescribing statins显示文摘 | Bader T | | 0，，： | 1 |
| 8 | Role of calcineurin in stress resistance,morphogenesis,and virulence of a Candida albicans wild-type strain显示文摘 | Bader T Schroppel K Bentink S | 2006 | Infect Immun2006，74，7： | 1 |
| 9 | The flow of phosphorous in food production and consumption-Linkoping,Sweden, 1870- 2000 显示文摘 | Neset T S S Bader H P Scheidegger R | 2008 | Science of the Total Environment2008，396，23： | 1 |
| 10 | Exposure to nitroaromat- ic explosives and health effects during disposal of military waste 显示文摘 | Letzel S Goen T Bader M | 2003 | Occupational and Environmental Medicine2003，60，： | 1 |
| 11 | Microbes, enzymes and genes in dichloromethane utilization显示文摘 | LEISINGER T BADER R HERMANN R | 1994 | Biodegration1994，5，： | 1 |
| 12 | Intensified surveillance after surgery for colorectal cancer significantly improves survival 显示文摘 | Laubert T Bader F G Oevermann E | 2010 | Eur J Med Res2010，15，1： | 1 |
| 13 | Tissue engineering of heart valves-human endothelial cell seeding of detergent acellularized porcine valves显示文摘 | Bader A Schilling T Teebken OE | 1998 | Eur J Cardiothorac Surg1998，14，3： | 1 |
| 14 | Dynamic compression count- er acts IL-lbeta induced iNOS and COX-2 activity by humanchom drocytes cultured in agarose constructs显示文摘 | CHOWDHURY T T BADER D L | 2006 | Biorheolo-gy2006，43，34： | 1 |
| 15 | Tensor decompositions and applications显示文摘 | KOLDA T G BADER B W | | 0，，3： | 1 |
| 16 | Tissue engineering of heart valves - human endothelial cell seeding of detergent acellularized porcine valves 显示文摘 | Bader A Schilling T Teebken OE | 1998 | Eur J Cardiothorac Surg1998，14，3： | 1 |
| 17 | A mechanism for increased plant growth induced by Trichoderma spp显示文摘 | Windham M T Elad Y Bader R | | 0，，： | 1 |
| 18 | Properties of Atoms in Molecules:Atomic Volumes显示文摘 | BADER R F W CARROLL M T CHEESEMAN J R | 1987 | J Am Chem Soc1987，109，： | 1 |
| 19 | Tensor decompositions andapplications显示文摘 | Kolda T G Bader B W | 2009 | SIAM Review2009，51，3： | 1 |
| 20 | Outcome analysis of elderly patients undergoing laparoscopic resection rectopexy forrectal prolapse显示文摘 | Laubert T Bader FG Kleemann M | 2012 | Int J Colorectal Dis2012，27，6： | 1 |
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