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| 1 | Factors predicting poor prognosis in ischemic colitis显示文摘瞄准:决定临床、分析、内视镜的因素与有关是化学家大肠炎(IC ) 严厉。方法:85 个病人的一个总数从 1996 年 1 月在回顾的研究被注册到 2004 年 5 月。有 53 女性和 32 男性(年龄 74.6+/-9.4 年,范围 45-89 年) 。病人作为 IC 被诊断。下列变量包括年龄,性别,从到承认的症状的外观的时间的经期,病历,药,凳子频率,临床的症状和症状被分析,验血(血克和基本生物化学的侧面) ,和内视镜的调查结果。病人们在温和 IC 组和严重 IC 组(外科或死亡) 被划分。质的变量用 chi 平方测试被分析,参量的数据用学生的 t (P<0.05 ) 被分析。结果:温和 IC 组 69 个病人被在于(42 女性和 27 男性,平均年龄 74.7+/-12.4 年) 。严重 IC 组由 16 个病人组成(11 女性和 5 男性,平均 73.8+/-12.4 年的年龄) 。因为麦克匪特斯氏疗法(没有外科) 的失败,一个病人死了, 15 个病人经历了外科(6 在内视镜的诊断以后并且 9 在 peroperatory 以后诊断) 。85 个病人(9.6%) 中的八个死了,其它被跟随在上面作为为 9.6+/-3.5 瞬间的门诊病人。人口统计的数据,病历,药和凳子频率在两个组(P>0.05 ) 是类似的。说正经的有病的病人比稍微有病的病人有更少的便血(37.5% 对 86.9% , P = 0.000 ) 。更多的心悸亢进(45.4% 对 10.1% , P = 0.011 ) 并且假腹膜炎的更高的流行签名(75% 对 5.7% , P = 0.000 ) 当腹的疼痛的存在和紧张在二个组之间是类似的时,在严重 IC 组被观察。当承认时,有严重 IC 的二个病人有吃惊。关于分析数据,更严重有病的病人被发现比略微有病的病人有贫血症和血钠过少(37.5% 对 10.1% , P = 0.014 和 46.6% 对 14.9% , P = 0.012,分别地) 。狭窄是那比在稍微有病的病人更经常出现在严重有病的病人的唯一的内视镜的发现(66.6% 对 17.3% , P = 0.017 ) 。结论:能预言 IC 的差的预后是便血,心悸亢进和假腹膜炎,贫血症和血钠过少和狭窄的缺席的因素。 | Ramón A■ón Marta Maia Boscá Vicente Sanchiz Joan Tosca Pedro Almela Cirilo Amorós Adolfo Benages | 2006 | World Journal of Gastroenterology2006，12，30： | 21 |
| 2 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 3 | Algal Biomass and Sea Surface Temperature in the Mediterranean Basin-intercomparison of Data from Various Satellite Sensors,and Implications for Primary Production Estimates显示文摘 | Bricaud A Bosc E Antoine D | 2002 | Rem Sens Env2002，81，23： | 1 |
| 4 | Variability of stem and branch maintenance respiration in a Pinus pinastertree显示文摘 | Bosc A de Grandcourt A Loustau D | 2003 | Tree Physiology2003，23，4： | 1 |
| 5 | Severe pneumonia due to cytomegalovirus in chronic obstructive pulmonary disease显示文摘 | Bosc C Clement M Deroux A | 2014 | Rev Mal Respir2014，31，5： | 1 |
| 6 | Empty versus overabundant answers to flexible relational queries 显示文摘 | Bosc P Hadjali A Pivert O | 2008 | Fuzzy Sets and Systems2008，159，12： | 1 |
| 7 | Hemifacial spasm:over 10 years of treatment with botuiinum toxin显示文摘 | Pérez-Salda(o)a MT Parkhutik V Boscá-Blasco ME | | 0，，10： | 1 |
| 8 | Impact of severe dry season on net ecosystem exchange in the Neotropical rain- forest of French Guiana显示文摘 | BONAL D BOSC A PONTON S eta | 2008 | Global Change Biology2008，14，8： | 1 |
| 9 | Severe pneumonia due to cytomegalovirus in chronic obstructive pulmonary disease显示文摘 | Bosc C Clement M Deroux A | 2014 | Rev Mal Respir2014，31，5： | 1 |
| 10 | Pro- staglandin E2 promotes migration and adhesion in hepa- tocellular carcinoma cells 显示文摘 | Mayoral R Fernendez-Martenez A Bosce L | 2005 | Carcinogenesis2005，26，4： | 1 |
| 11 | Do noradrenaline and serotonin differentially affect social motivation and behaviour?显示文摘 | Dubini A Bosc M Polin V | 1997 | Eur Neuropsychopharmacol1997，7，1： | 1 |
| 12 | EMILION, a tree functional - structural model: presentation and first application to the analysis of branch carbon balance 显示文摘 | Bosc A | 2000 | Annals of Forest Science2000，57，56： | 1 |
| 13 | 8-O-Azeloyl-14-benzoylaconine:a new alkaloid from the roots of Aconitum karacolicum Rapcs and its antiproliferative activities显示文摘 | Chodoeva A Bosc J J Guillon J | 2005 | Bioorg Med Chem2005，13，23： | 1 |
| 14 | Hemisynthesis and antiproliferative properties of mono-esters and bis-esters显示文摘 | Chodoeva A Bosc J J Guillon J | 2012 | Eur J Med Chem2012，54，： | 1 |
| 15 | Antitumor activity of semisynthetic derivatives of Aconitum alkaloids显示文摘 | Chodoeva A Bosc J J Lartigue L | 2014 | Invest New Drugs2014，32，1： | 1 |
| 16 | A simple route for low-temperature synthesis of mesoporous and nanocrystalline anatase thin films显示文摘 | Bosc F Ayral A Albouy P A | 2003 | Chem Mater2003，15，： | 1 |
| 17 | A cost analysis of DIEP flap in breast reconstruction 显示文摘 | Marchac A Bosc R Benjoar MD | 2011 | Ann Chir Plast Esthet2011，56，4： | 1 |
| 18 | Severe pneumonia due to cytomegalovirus in chronic obstructive pulmonary disease 显示文摘 | Bosc C Clement M Deroux A | 2014 | Rev nal Respir2014，31，5： | 1 |
| 19 | On database queries involving competitive conditional preferences显示文摘 | Bosc P Hadjali A Pivert O | 2011 | International Journal of Intelligent Systems2011，6，3： | 1 |
| 20 | Transgenic Mice Expressing Cyclooxygenase-2 in Hepatocytes Reveal a Minor Contribution of This Enzyme to Chemical Hepatocarcinogenesis显示文摘 | Cristina Llorente Izquierdo Rafael Mayoral Juana María Flores Pilar García-Palencia Carme Cucarella Lisardo Boscá Marta Casado Paloma Martín-Sanz | 2011 | The American Journal of Pathology2011，，3： | 1 |
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