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| 1 | 褐煤干燥新方法:利用内部余热的沸腾床干燥法显示文摘 | Bockes,D 刘成勇 | 1991 | 世界煤炭技术1991，，4： | 4 |
| 2 | Molecular phenotypes of human parvovirus B19 in patients with myocarditis显示文摘AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional experiments.EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers.Control tissues were obtained at autopsy from 34 victims of accidents,crime or suicide.Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining.Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments.Transfection experiments were conducted using human endothelial cells 1.Luciferase reporter assays were performed to determine B19Vreplication activity.Statistical analysis and graphical representation were calculated using SPSS and Prism5 software.RESULTS:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was present.RFLP-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B19V-genotype 2(44.6%) than men(36.0%)(P = 0.0448).Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissuesamples and was associated with higher B19V viral load compared to B19V-monoinfected tissue(P = 0.0012).The most frequent coinfecting virus was human herpes virus 6(HHV6,16.5%).B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs(P = 0.0033),suggesting that HHV6 had transactivated B19V.In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator.CONCLUSION:The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP.Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype. | C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy Karin Klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf | 2014 | World Journal of Cardiology2014，6，4： | 3 |
| 3 | General aspects and limits of conventional ultrafine WC powder manufacture and hard metal production显示文摘 | Schubert W D Bock A Lux B | 1995 | Int J Refract Met Hard Mat1995，13，： | 1 |
| 4 | The mouse homolog of the orphan nuclear receptor tailless is expressed in the developing forebrain显示文摘 | Monaghan AP Grau E Bock D | 1995 | Development1995，121，3： | 1 |
| 5 | ESKA: an expert system for the selection and optimization of catalysts显示文摘 | Bock W Haho V etal | 1989 | Decheme-Monogr1989，116，： | 1 |
| 6 | ESKA:an expert system for the selection and optimization of catalysts显示文摘 | Arntz D Bock W HahoV | 1989 | Decheme-Monogr1989，，16： | 1 |
| 7 | Microanalysis of museum aerosols to elucidate the soiling of paintings: Case of the Correr Museum, Venice, Italy显示文摘 | De Bock L A van Grieken R E Camuffo D | 1996 | Environmental Science & Technology1996，30，11： | 1 |
| 8 | Anatomy of Apparent Seasonal Variations from GPS Derived Site Position Time Series 显示文摘 | DONG D FANG P BOCK Y | 2002 | JGR2002，107，4： | 1 |
| 9 | Microanalysis of museum aerosols to elucidate the soiling of paintings: case of the Correr Museum, Venice, Italy显示文摘 | De Bock L A Grieken R E V Camuffo D | 1996 | Envir Sci Technol1996，30，： | 1 |
| 10 | General aspects and limits of conventional ultrafine WC powder manufacture an hard metal production显示文摘 | Schubert W D Bock A Lux B | 1995 | Int J Refract Met Hard Mater1995，13，5： | 1 |
| 11 | Identification of the transcriptional activator controlling the butanediol fermentation pathway in Klebsiella terrigena显示文摘 | MAYER D SCHLENSOG V BOCK A | 1995 | Journal of Bacteriology1995，177，18： | 1 |
| 12 | Rapid production of microwave packaging in Silicon-aluminium by thin-shell electroforming显示文摘 | BOCKING C JACOBSON D M RENNIE A EW | 2002 | Microelectronics International2002，19，1： | 1 |
| 13 | Anatomy of Apparent Seasonal Variations from GPS-derived Site Position Time Series显示文摘 | Dong D Fang P Bock Y | 2002 | Journal of Geophysical Research2002，107，4： | 1 |
| 14 | Rational de- sign of novel, potent small molecule parvselectin antaqonlsts 显示文摘 | KRANICH R BUSEMANN A S BOCK D | 2007 | J Med Chem2007，50，17： | 1 |
| 15 | Identification of spore aller- gens from the indoor mould Aspergillus versicolor显示文摘 | Benndorf D Miiller A Bock K | 2008 | Allergy2008，63，4： | 1 |
| 16 | Global Positioning System Net- work Analysis with Phase Ambiguity Resolution Applied to Crustal Deformation Studies in California显示文摘 | Dong D N Bock Y | | 0，，4： | 1 |
| 17 | General aspects and limits of conventional ultra_fine WC powder manufacture and hard metal production 显示文摘 | Shubert W D Bock A Lux B | 1995 | International Journal of Refractory Metals &Hard Matcrials1995，13，5： | 1 |
| 18 | Intravesical bacillus Calmette-Guerin versus mitomycin C for superficial bladder cancer: a for-mal meta-analysis of comparative studies on recurrence and toxici-ty显示文摘 | Bohle A Jocham D Bock PR | 2003 | J UroU2003，169，1： | 1 |
| 19 | Study of seasonal-scale atmospheric water cycle with ground-based GPS receivers, radiosondes and NWP models over Morocco 显示文摘 | KOULALI A OUAZAR D BOCK 0 | 2012 | Atmospheric Research2012，104，： | 1 |
| 20 | Anatomy of apparent seasonal variations from GPS derived site position time se- ries 显示文摘 | Dong D N Fang P Bock Y | 2002 | Journal of Geophysical Research2002，107，4： | 1 |
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