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| 1 | Bile leakage after loop closure vs clip closure of the cystic duct during laparoscopic cholecystectomy:A retrospective analysis of a prospective cohort显示文摘BACKGROUND Laparoscopic cholecystectomy(LC)is one of the most frequently performed surgical procedures.Cystic stump leakage is an underestimated,potentially life threatening complication that occurs in 1%-6%of the patients.With a secure cystic duct occlusion technique during LC,bile leakage becomes a preventable complication.AIM To investigate the effect of polydioxanone(PDS)loop closure of the cystic duct on bile leakage rate in LC patients.METHODS In this retrospective analysis of a prospective cohort,the effect of PDS loop closure of the cystic duct on bile leakage complication was compared to patients with conventional clip closure.Logistic regression analysis was used to develop a risk score to identify bile leakage risk.Leakage rate was assessed for categories of patients with increasing levels of bile leakage risk.RESULTS Of the 4359 patients who underwent LC,136(3%)underwent cystic duct closure by a PDS loop.Preoperatively,loop closure patients had significantly more complicated biliary disease compared to the clipped closure patients.In the loop closure cohort,zero(0%)bile leakage occurred compared to 59 of 4223(1.4%)clip closure patients.For patients at increased bile leakage risk(risk score≥1)rates were 1.6%and up to 13%(4/30)for clip closure patients with a risk score≥4.This risk increase paralleled a stepwise increase of actual bile leakage complication for clip closure patients,which was not observed for loop closure patients.CONCLUSION Cystic duct closure with a PDS loop during LC may reduce bile leakage in patients at increased risk for bile leakage. | Sandra C Donkervoort Lea M Dijksman Aafke H van Dijk Emile A Clous Marja A Boermeester Bert van Ramshorst Djamila Boerma | 2020 | World Journal of Gastrointestinal Surgery2020，12，1： | 4 |
| 2 | Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy显示文摘AIM:To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery(CPS)and femtosecond laserassisted cataract surgery(FLACS).METHODS:The cytoskeleton of the epithelial cells of the anterior lens capsules(ALC)removed by CPS and FLACS was examined by immunohistochemistry.Expression of the intermediate filament,glial fibrillary acidic protein(GFAP),and glutamine synthetase(GS)immunoreactivity were detected.In order to map the actin network of cells,fluorescently labeled phalloidin was used.The samples were examined using confocal laser scanning microscopy.RESULTS:GFAP expression was visible in a larger number of the epithelial cells after CPS compared to FLACS.In CPS sample’s epithelial cells,GFAP immunoreactivity indicated robust morphological change.Regarding the actin filaments,the presence of tubular elements connecting epithelial cells,regular actin pattern and marked cortical network after CPS were found.Following FLACS,the actin cytoskeleton of the epithelial cells remained densely structured,and the tubular elements were undetectable,however,the above-mentioned regular actin pattern and the marked cortical network were visible.CONCLUSION:The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells. | ANDrea Krisztina Sükosd Krisztina Szabadfi Edina Szabó-Meleg Beáta Gáspár Pavel Stodulka Gyorgy SétálóJr Róbert Gábriel Miklós Nyitrai Zsolt Biró Hajnalka Abrahám | 2020 | International Journal of Ophthalmology(English edition)2020，13，6： | 2 |
| 3 | Raman spectroscopy employed for the determination of the intermediate phase in polyethylene显示文摘 | CHRISTOPHER C N ROBERT J M BERT J K et al | 1995 | Macromolecules1995，28，8： | 1 |
| 4 | Selenium redox biochemistry of zinc - sulfur co- ordination sites in proteins and enzymes显示文摘 | Claus J wolfgang M Bert V | 1999 | Proc Natl Acad Sci USA1999，96，： | 1 |
| 5 | The differential quadrature method in computational mechanics: A review显示文摘 | Bert C W Malik M | 1996 | Appl Mech Rev1996，，： | 1 |
| 6 | Application of viscous pressure forming(VPF) to low volume stamping of difficult-to-form alloys-results of preliminary FEM simulations显示文摘 | Liu Junhua Bert Westhoff Ahmetoglu M | 1996 | J Mater Proce Tec1996，，59： | 1 |
| 7 | Hydrostatic pressure and cellular respiration:are the values observed post-decompression representative of the reality under pressure?显示文摘 | Theron M Sébert P | 2003 | Mitochondrion2003，3，2： | 1 |
| 8 | The role of positive interactions in communities: Lessons from intertidal habitats显示文摘 | BERTNESS M D LEONARD G H | 1997 | Ecology1997，78，： | 1 |
| 9 | Zinc Tolerance and Accumulation in Metallicolous and Nonmetallicolous Populations of Arabidopsis Halleri (Brassicaceae) 显示文摘 | MACNAIR M R DELAGUERIE P | 2000 | New Phytologist2000，146，： | 1 |
| 10 | SIMULSERRE:an educational software simulating the greenhouse-crop systems显示文摘 | Gary C Tchamitchian M Bert in N | 1998 | Acta Horticulturae1998，45，6： | 1 |
| 11 | Implementting multiple boundary conditions in the DQ solution of hingher-order PDE's: application to free vibration of plates显示文摘 | Malik M Bert C W | 1996 | International Journal for Numerical Methods in Engineering1996，39，7： | 1 |
| 12 | Renal pro- tection in diabetes: role of glycemic control 显示文摘 | FIORETTO P BRUSEGHIN M BERT() I | 2006 | J Am Soc Nephrol2006，17，： | 1 |
| 13 | Zinc tolerance and accumulation in metallicolous populations of Arabi dopsis halleri (Brassicaceae)显示文摘 | Bert V Macnair M R Lagurie P D E | 2000 | New Phytologist2000，，146： | 1 |
| 14 | Physical and bioticdrivers of plant distribution across estuarine salinity gradients显示文摘 | Crain C M Silliman B R Bertness S L | 2004 | Ecology2004，85，： | 1 |
| 15 | Regional Activation of the Cancer Genome by Long-Range Epigenetie Remodeling显示文摘 | BERT S A ROBINSON M D STRBENAC D | 2012 | Cancer Cell2012，23，1： | 1 |
| 16 | Solution of the improved and generalized quasistatic methods using an analytic calculation or a semiimplicit scheme to compute the precursor equations显示文摘 | KOCLAS J SISSAOUI M T H BERT A | 1996 | Ann Nucl Energy1996，23，11： | 1 |
| 17 | Differential quadrature solutions for steady-slate incompressible and compressible lubrication prob-lems显示文摘 | Malik M Bert C W | 1994 | Journal of Tribology1994，116，： | 1 |
| 18 | Interspecific interactions among high marsh perennials in a New England salt marsh显示文摘 | BERTNESS M D | 1991 | Ecology1991，72，： | 1 |
| 19 | Differential Quadrature Method in Computational Mechanics : A Review 显示文摘 | Bert C W Malik M | 1996 | Applied Mechanics Reviews1996，49，： | 1 |
| 20 | Evaluation of the Osiris expert system for identification of beta-lactam phenotypes in isolates of Pseudomonas aeruginosa显示文摘 | Bert F Ould-Hocine Z Juvin M | | 0，，： | 1 |
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