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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | Biocorrosion: towards understanding interactions between biofilms and metals显示文摘 | Iwona B Beech Jan Sunner | 2004 | Current Opinion in Biotechnology2004，，3： | 2 |
| 3 | Atomic force microscopy study of microbiological influenced corrosion SRB biofilms on steel surface显示文摘 | Steele A Goddard D T Beech I B | 1994 | International Biodeterioration and Biodegradation1994，34，： | 1 |
| 4 | Biocorrosion : towards understand- ing interactions between biofilms and metals 显示文摘 | Beech I B Sunner J | 2004 | Curr Opin Biotechnol2004，15，3： | 1 |
| 5 | Workplace violence in the health care: A review of staff training and integration of training evaluation models 显示文摘 | Beech B Leather P | 2006 | Aggression and Violent Behavior2006，11，： | 1 |
| 6 | The production of strawberry plants from callus culture 显示文摘 | Jones O P Waller B J Beech M G | 1988 | Plant Cell Tissue and Organ Cult1988，12，33： | 1 |
| 7 | The BioCassava plus program:biofortification of cassava for sub-Saharan Africa显示文摘 | SAYRE R BEECHING J R CAHOON E B | 2011 | Annual Review of Plant Biology2011，62，： | 1 |
| 8 | Towards understanding interaction between biofi/ms and metals , Current Opinion in biotechnology 显示文摘 | Beech I B Sunner J | 2004 | Biocorrosion2004，15，3： | 1 |
| 9 | Sulfate-reducing bacteria in biofilms on metallic materials and corrosion显示文摘 | Beech I B | 2003 | Microbiology Today2003，30，3： | 1 |
| 10 | Corrosion of technical materials in the presence of biofilms current understanding and state of theart methods of study显示文摘 | Beech I B | 2004 | International Biodeterioration & Biodegradation2004，53，： | 1 |
| 11 | Corrosion of technical materials in the presence of bio- films-current understanding and state of the art methods of study 显示文摘 | BEECH I B | 2004 | International Biodeterioration & Biodegradation2004，53，3： | 1 |
| 12 | Workplace violence in the health acre sector:A review of staff training and integration of training evaluation models显示文摘 | Beech B Leather P | 2006 | Aggression and Violent Behavior2006，，11： | 1 |
| 13 | Bioeorrosion: towards understanding interactions between biofilms and metals显示文摘 | BEECH I B SUNNER J | 2004 | Current Opinion in Bioteehnology2004，15，3： | 1 |
| 14 | Ibuprofen suppresses plaque pathology and inflammation in a mouse model for Alzheimer′s disease显示文摘 | Lim GP Yang F Chu T Chen P Beech W Teter B | 2000 | J Neurosci2000，20，15： | 1 |
| 15 | Interactions of exopolymers produced by Sulphate reducing bacteria with metal ions显示文摘 | I B Beech C W S Cheung | 1995 | International Biodeterioration & Biodegradation1995，38，： | 1 |
| 16 | Interactions of exopolymers pro duced by sulphate reducing bacteria with metal ions显示文摘 | Beech I B Cheung C W S | 1995 | In- ternational Biodeterioration and Biodegradation1995，35，123： | 1 |
| 17 | Sulfate-reducing bacteria in biofilms on metallic materials and corrosion显示文摘 | Beech I B | 2003 | Microbiol Today2003，30，： | 1 |
| 18 | Workplace violence in the health acre sector: A review of staff training and integration of training evaluation models 显示文摘 | BEECH B LEATHER P | 2006 | Agg Viol Beh2006，11，： | 1 |
| 19 | Go the extra mile-use the Delphi technique 显示文摘 | Beech B | 1999 | J Nurse Manage1999，7，5： | 1 |
| 20 | Biocorrosion:towards understanding interactions between biofilms and metals显示文摘 | I B Beech J Sunner | 2004 | Current opinion in biotechnology2004，15，： | 1 |
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