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| 1 | Liver-specific gene expression in mesenchymal stem cells is induced by liver cells显示文摘AIM: The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stem cell cultures. We investigated the potential of rat mesenchymal stem cells (MSC) from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore,we assessed the influence of cocultured liver cells on induction of liver-specific gene expression.METHODS: Mesenchymal stem cells were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF,EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thy1 and the hepatocytic markers CK-18, albumin, CK-19,and AFP was performed in the different cell populations.RESULTS: Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18,CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression.CONCLUSION: The results indicate that (1) rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and (2) that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC. | Claudia Lange Philipp Bassler Michael V. Lioznov Helge Bruns Dietrich Kluth Axel R. Zander Henning C. Fiegel | 2005 | World Journal of Gastroenterology2005，11，29： | 31 |
| 2 | Chemical communication among bacteria显示文摘 | Taga M E Bassler B L | 2003 | PNAS2003，100，14： | 1 |
| 3 | Organ - preserving and reconstructive microsurgery of the fallopian tubes in tub- al infertility: still an alternative to in vitro fertilization (IVF) 显示文摘 | Schippert C Hille U Bassler C | 2010 | J Reconstr Microsurg2010，26，5： | 1 |
| 4 | Early interventions involving parents to improve neurodevelopmental outcomes of premature infants : a meta-analysis显示文摘 | Bassler D Robertson CMT Kirpalani H | 2009 | J Perinatol2009，29，5： | 1 |
| 5 | ERCP and MRCP in the differentiation of pancreatic tumors 显示文摘 | Hartmann D Schilling D Bassler B | 2004 | Dig Dis2004，22，1： | 1 |
| 6 | Reconstructive, or- gan- preserving microsurgery in tubal infertility: still an al- ternative to in vitro fertilization 显示文摘 | Schippert C Bassler C Soergel P | 2010 | Fertil Steril2010，93，4： | 1 |
| 7 | QUORUM SENSING IN BACTERIA显示文摘 | Melissa B. Miller Bonnie L. Bassler | 2001 | Annual Review of Microbiology2001，，： | 1 |
| 8 | Evaluating the evidence for assessing BNP and NT-proBNP levels显示文摘 | Busse JW Bassler D Guyatt GH | 2008 | Clin Biochem2008，41，45： | 1 |
| 9 | Bacterial quorum-sensing network architectures显示文摘 | Ng W L Bassler B L | 2009 | Annual Review of Genetics2009，43，： | 1 |
| 10 | Caffeine for apnea of prematurity: a neonatal success story显示文摘 | Kxeutzer K Bassler D | 2014 | Neonatology2014，105，4： | 1 |
| 11 | Quorum sensing: cell-to-cell communication in bacteria显示文摘 | Waters CM Bassler BL | 2005 | Annual Review of Cell and Developmental Biology2005，21，1： | 1 |
| 12 | Quorum sensing in Escherichia coli,Salmonella typhimurium,and Vibrio harveyi:a new family of genes responsible for autoinducer production显示文摘 | Surette MG Miller MB Bassler BL | 1999 | Proc Natl Acad Sci U S A1999，96，4： | 1 |
| 13 | Multiple signalin systems controlling expression of luminescence in Vibrio harveyi:Sequence and function of genes encoding a second sensory pathway显示文摘 | Silverman MR | 1994 | Mol Microbiol1994，13，： | 1 |
| 14 | Chemical communication among bacteria显示文摘 | TAGA E M BASSLER L B | 2003 | Proceedings of the National Academy of Sciences of the United States of America2003，100，14： | 1 |
| 15 | Quorum sensing in bacteria显示文摘 | Miller MB Bassler BL | 2001 | Ann Rev Microbiol2001，55，： | 1 |
| 16 | The languages of bacteria 显示文摘 | Schauder S Bassler B L | 2001 | Genes Dev2001，15，2： | 1 |
| 17 | Bacterial quorum sensing:Its role in virulence and possibilities for its control显示文摘 | Rutherford S T Bassler B L | 2012 | Cold Spring Harbor Perspectives in Medicine2012，，： | 1 |
| 18 | Bacterially speaking显示文摘 | Bassler B L Losick R | 2006 | Cell2006，125，2： | 1 |
| 19 | Quorum sensing: CCell-to-cell communication in bacteria显示文摘 | Waters CM Bassler BL | 2005 | Annu Rev Cell Dev Biol2005，21，： | 1 |
| 20 | Quorum sensing in bacteria 显示文摘 | Miller MB Bassler BL | 2001 | Annu Rev Microbiol2001，55，： | 1 |
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