| 6 | A versatile approach for temporary storage and shipping of in vitro cultured cells,cell sheets and tissue engineered constructs-a preliminary report显示文摘Temporary storage/shipping of cell/tissue engineering products from bench to bedside is a key aspect of re-generative medicine.The current proof-of-concept study presents a multipurpose device for temporary storage/shipping of cell culture dishes containing cell/tissue constructs.The device,made with readily available raw ma-terials,contains three elements viz.a specialized lid,polymeric plates having grooves and a set of nuts and bolts.As part of the performance evaluation,the device was first subjected to a simulated storage/shipping process,wherein the leak-proof and aseptic containment of the contents was demonstrated.Subsequently,the setup was used for temporary storage/shipping of dishes having(a)L929 cell monolayers cultured on treated surfaces,(b)SIRC,HaCaT and A549 cell sheets cultured on thermo-responsive surfaces,(c)HOS-cell encapsulated agar gels and(d)HOS-cell seeded silk fibroin mats.The results showed that the health of cell monolayers/cell sheets/tissue constructs after the process was comparable to that before the process.The device was scalable,simple to handle,can be made for a single or multi-use purpose,and can be resizable to load other culture vessels.The design of the storage/shipping device described in this report thus offers versatile features and applications. | Anju MS Athira RK Ramesh Babu V Anil Kumar PR Naresh Kasoju | 2022 | Engineered Regeneration2022，3，3： | 0 |
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