|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Physical chemical and biological characterization of a new bacteriocin produced by Bacillus cereus NS02显示文摘Objective:To screen the bacteriocinogenic isolate from buffalo milk and to characterize it on physical,chemical and biological aspects for the application in biopreservation.Methods:Bacillus cereus(B.cereus)was isolated and assessed for its baceteriocinogenic activity.Bacteriocin was produced and purified by ammonium sulphate precipitation,dialysis and gel filtration chromatography.Purified bacteriocin was used to clieck its antimicrobial activity against food borne bacteria.Effect and stability of bacteripcin was determined with the respect to temperature,pH,enzymes,organic solvents and chemicals.Bacteriocin was also subjected to SDS PAGE analysis to determine its molecular weight.In addition,functional groups exist in the bacteriocin was determined by FTIR analysis.Results:B.cereus was identified by 16S rRNA sequence analysis.Bacteriocin showed increased activity against all the bacteria used and its activity unit was found to be 51,200 AU/mL.It was stable to high temperature(100℃)and wide range of pH(3-10),sensitive to proteolytic enzymes and resistant to nonprotcolytic enzymes.It was low molecular weight(3.5-6 kDa)protein and FTIR study revealed the presence of amide group and NH stretching,Conclusions:Bacteriocin produced in this study possesses the highest antimicrobial activity against both gram positive and gram negative bacteria thereby it has immense application as biopreservative agent.FTIR proved its peptide nature. | Senbagam D Gurusamy R Senthilkumar B | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，12： | 4 |
| 2 | Hepatoprotective potential of petroleum ether leaf extract of Crossandra infundibuliformis on CCl_4 induced liver toxicity in albino mice显示文摘Objective:To analyze the hepatoprotective effect of the Crossandra infundibuliformis.Methods: Hepatotoxicity was induced by carbon tetrachloride.Petroleum ether extract of dried leaves was administrated to mice for 7 days.The hepatoprotective effect of petroleum ether extract was evaluated by the assay of liver function biochemical parameters.Results:The result clearly indicates that petroleum ether extract showed significant hepatoprotection when compared with standard Silumarin.Conclusions:The petroleum ether extract of the leaves of Crossandra infundibuliformis possess significant acute hepatoprotective activity.Thus further investigation on this species would bring a promising drug for liver disorders. | G Madhumitha A Mary Saral B Senthilkumar A Sivaraj | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，10： | 2 |
| 3 | Induction of deletion mutation on ompR gene of Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development显示文摘Objective:To develop allenualed slrains of Salmonella enterica serorar Typhi(S.typhi) for the candidate vaccine by osmolar stress.Mothods:S.typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil Nadu.India.Both strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) respectively.The effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of S.typhi SS3 and SS5 strains.Attenuation by osmolar stress results in deletion mutation of the.S.typhi slrains was determined by agglutination assays,precipitation method.SDS PAGE analysis and by animal models.Results:The 799 bp amplified ompR gene product from wild type S.typhi SS3 and SS5 illustrate the presence of virulent gene.Interestingly,there was only a 282 bp amplified product from S.typhi SS3 and SS5 grown in the presence of 0.5.0.6 and 0.7 M NaCl.This illustrates the occurrence of deletion mutation in ompR gene al high concentration of NaCl.Furthermore,both the wildtype and mutant S.typhi outer membrane SDS-PAGF.profile reveals the differences in the expression of ompF.ompC and ompA proteins.In mice,wild type and mutant strains lethal dose(LD_(50)) were determined.The mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL^(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune response.These results suggest that S.typhi SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against salmonellosis.Conclusions:This study demonstrates that the S.typhi strains were allenualed and could be good vaccine candidates in future. | Senthilkumar B Anbarasu K Senbagam D Rajasekarapandian M | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，12： | 2 |
| 4 | Synthesis,characterization and thermal studied on furazan-and tetrazine-based high energy materials显示文摘 | M B Talawar R Sivabalan N Senthilkumar | 2004 | J Hazarous Materials2004，113，： | 1 |
| 5 | Studies on azotetrazolate based high nitrogen content high energy materials potential additives for rocket propellants显示文摘 | Sivabalan R Talawar M B Senthilkumar N | 2004 | Journal of Thermal Analysis and Calorimetry2004，78，： | 1 |
| 6 | Production of bacteriocin and their application in food products 显示文摘 | Udhayashree N Senbagam D Senthilkumar B | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，： | 1 |
| 7 | Prepara- tion of activated carbon from sorghum pith and its struc- tural and electrochemical properties显示文摘 | Senthilkumar S T Senthilkumar B Balaji S | 2011 | Materials Re- search Bulletin2011，46，3： | 1 |
| 8 | Synthesis,characterization and thermal studies on furazan-and tetrazine-based high energy materials显示文摘 | M B Talawar R Sivabalan N Senthilkumar | 2004 | Journal of Hazardous Materials2004，113，： | 1 |
| 9 | Experimental quantification of chemical effects of hydrodynamic cavitation 显示文摘 | Senthilkumar P Sivakumar M Pandit A B | 2000 | Chemical Engineering Science2000，55，9： | 1 |
| 10 | Compari-son of patient-controlled analgesia with and without dexmedetomidine following spine surgery in children显示文摘 | SENTHILKUMAR S ANNE B MOHAMED M | 2009 | J Clin Anesth2009，21，7： | 1 |
| 11 | Studies on azotetrazolate based high nitrogen content high energy materials potential additives for rocket propellants 显示文摘 | Sivabalan R Talawar M B Senthilkumar N | 2004 | Journal of Thermal Analysis and Calorimetry2004，78，8： | 1 |
| 12 | De- repressed 2-deoxyglucose-resistant mutants of Aspergillus niger with altered hexokinase and acid phosphatase activity in hyperproduction of β-Fructofuranosidase显示文摘 | B Ashokkumar SR Senthilkumar P Gunasekaran | 2004 | Applied biochemistry and biotechnology2004，118，13： | 1 |
| 13 | Process factor optimization for controlling pull-down defects in iron castings显示文摘 | Senthilkumar B Ponnambalamb S G Jawahar N | 2009 | Journal of Materials Proceedsing Technology2009，209，： | 1 |
| 14 | Synthesis, characterization and thermal studies on furazan- and tetrazine-based high energy materials显示文摘 | Talawar M B Sivabalan R Senthilkumar N | 2004 | Journal of Hazardous Materials2004，113，13： | 1 |
| 15 | Studies on azotetrazolate based high nitrogen content high energy materials potential additives for rocket p ropellants显示文摘 | Sivabalan R Talawar M B Senthilkumar N | 2004 | Journal of thermal analysis and calorimetry2004，78，： | 1 |
| 16 | Sirt3 blocks the cardiac hypertrophic response by augmenting Foxo3a-dependent antioxidant defense mechanisms in mice显示文摘 | Sundaresan Nagalingam R Gupta Madhu Kim Gene Rajamohan Senthilkumar B Isbatan Ayman Gupta Mahesh P | 2009 | Journal of Clinical Investigation2009，，9： | 1 |
| 17 | Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design显示文摘 | Senthilkumar S R Ashokkumar B Chandra Raj K | 2005 | Bioresou Technol2005，96，： | 1 |
| 18 | Structural and electrochemical properties of polythiophene显示文摘 | Senthilkumar B Thenamirtham P Kalai Selvan R | 2011 | Electrochim Acta2011，257，： | 1 |
| 19 | Hepatoprotective activity of Indian Phyllanthus显示文摘 | Srirama R) Deepak H B Senthilkumar U | 2012 | Pharmaceutical Biology2012，50，8： | 1 |
| 20 | Analysis on drilling of glass fiber-reinforced polymer(GFRP) composites using grey relational analysis 显示文摘 | Palanikumar K Latha B Senthilkumar V S | 2012 | Materials and Manufacturing Processes2012，27，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员