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| 1 | Supramolecular Nanohelix Fabricated by Pillararene-Based Host–Guest System for Chirality Amplification, Transfer, and Circularly Polarized Luminescence in Water显示文摘Amplified chirality and Förster resonance energy transfer(FRET)-assisted chirality transfer frommolecular to nanoscale level have been shown to play a vital role in co-assembled nanohelix for potential energy transfer in biological systems.Herein,we have constructed a chiral host–guest complex donor system for chiral amplification via induced chirality of pillar[5]arene host and loaded it with an achiral dye acceptor to demonstrate how chirality-assisted excitation energy transfer occurred in the supramolecular nanohelix system in an aqueous medium. | Krishnasamy Velmurugan Adil Murtaza Azhar Saeed Jianing Li Kaiya Wang Minzan Zuo Qian Liu Xiao-Yu Hu | 2022 | CCS Chemistry2022，4，10： | 3 |
| 2 | Free fatty acids receptors 2 and 3 control cell proliferation by regulating cellular glucose uptake显示文摘BACKGROUND Colorectal cancer(CRC)is a worldwide problem,which has been associated with changes in diet and lifestyle pattern.As a result of colonic fermentation of dietary fibres,short chain free fatty acids are generated which activate free fatty acid receptors(FFAR)2 and 3.FFAR2 and FFAR3 genes are abundantly expressed in colonic epithelium and play an important role in the metabolic homeostasis of colonic epithelial cells.Earlier studies point to the involvement of FFAR2 in colorectal carcinogenesis.AIM To understand the role of short chain FFARs in CRC.METHODS Transcriptome analysis console software was used to analyse microarray data from CRC patients and cell lines.We employed short-hairpin RNA mediated down regulation of FFAR2 and FFAR3 genes,which was validated using quantitative real time polymerase chain reaction.Assays for glucose uptake and cyclic adenosine monophosphate(cAMP)generation was done along with immunofluorescence studies to study the effects of FFAR2/FFAR3 knockdown.For measuring cell proliferation,we employed real time electrical impedancebased assay available from xCELLigence.RESULTS Microarray data analysis of CRC patient samples showed a significant down regulation of FFAR2 gene expression.This prompted us to study the FFAR2 in CRC.Since,FFAR3 shares significant structural and functional homology with FFAR2,we knocked down both these receptors in CRC cell line HCT 116.These modified cell lines exhibited higher proliferation rate and were found to have increased glucose uptake as well as increased level of glucose transporter 1.Since,FFAR2 and FFAR3 signal through G protein subunit(Gαi),knockdown of these receptors was associated with increased cAMP.Inhibition of protein kinase A(PKA)did not alter the growth and proliferation of these cells indicating a mechanism independent of cAMP/PKA pathway.CONCLUSION Our results suggest role of FFAR2/FFAR3 genes in increased proliferation of colon cancer cells via enhanced glucose uptake and exclude the role of PKA mediated cAMP signalling.Alternate pathways could be involved that would ultimately result in increased cell proliferation as a result of down regulated FFAR2/FFAR3 genes.This study paves the way to understand the mechanism of action of short chain FFARs in CRC. | Saeed Al Mahri Amal Al Ghamdi Maaged Akiel Monira Al Aujan Sameer Mohammad Mohammad Azhar Aziz | 2020 | World Journal of Gastrointestinal Oncology2020，12，5： | 2 |
| 3 | Evidence for camel- to-human transmission of MERS coronavirus 显示文摘 | Azhar EI E1-Kafrawy SA Farraj SA Hassan AM A1- Saeed MS Hashem AM Madani TA | 2014 | N Engl J Med2014，370，26： | 1 |
| 4 | Pathogenicity of avian influenza virus H5N1 2007 isolates from Pakistan显示文摘 | Farhan Afzal Ali Saeed Muhammad Azhar Sharif Najma Ayub Shamsul Hassan | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，，1： | 1 |
| 5 | Intercomparison of Environmental Gamma doses Measured with A NaI(Tl) Survey Meter and Thermoluminescent Dosimeters(TLDs) in the Poonch Division of Azad Kashmir, Pakistan显示文摘This study presents the intercomparison of the outdoor environmental gamma dose rates measured using a Na I(Tl)based survey meter along with thermoluminescent dosimeters(TLDs)and estimation of excess lifetime cancer risk(ELCR),for the inhabitants of Poonch division of the | Muhammad Rafique Kimberlee J Kearfott Khalil Ahmad Jabeen Akhter Abdul Razzaq Khan Raja Azhar Saeed Saeed Ur Rahman Matiullah Muhammad Usman Rajput | 2014 | Biomedical and Environmental Sciences2014，27，12： | 0 |
| 6 | Helicobacter pylori outer membrane protein Q genotypes and their susceptibility to antiadhesive phytotherapeutic agents显示文摘OBJECTIVE: Helicobacter pylori is a Gram-negative organism. Its outer membrane protein Q(Hop Q) mediates host-pathogen interactions; Hop Q genotypes 1 and 2 are found associating with gastroduodenal pathologies. The authors measured the anti-adhesion effects of the extracts of Abelmoschus esculentus, Zingiber officinale, Trachyspermum ammi, Glycyrrhiza glabra, Curcuma longa and Capsicum annum against Hop Q genotypes and H. pylori cytotoxin-associated gene A(Cag A).METHODS: DNA was extracted by polymerase chain reaction of the Hop Q genotypes(i.e., type 1, type 2 and Cag A) from 115 H. pylori strains. The effect of the extracts from selected dietary ingredients was determined using a gastric adenocarcinoma cell line and a quantitative DNA fragmentation assay. The anti-adhesive effect of these extracts on H. pylori was tested using an anti-adhesion analysis.RESULTS: C. annum, C. longa and A. esculentus showed prominent anti-adhesion effects with resultant values of 17.3% ± 2.9%, 14.6% ± 3.7%, 13.8% ± 3.6%, respectively, against Hop Q type 1 and 13.1% ± 1.7%, 12.1% ± 2%, 11.1% ± 1.6%, respectively, against Hop Q type 2. C. longa(93%), C. annum(89%) and A. esculentus(75%) had better anti-adhesive activity against H. pylori with Hop Q type 1 compared to Hop Q type 2 with respective values of 70%, 64% and 51%. Extracts of C. annum(14.7% ± 4.1%), A. esculentus(12.3% ± 4.1%) and Z. officinale(8.4% ± 2.8%) had an anti-adhesion effect against Cag A-positive H. pylori strains compared to Cag A-negative strains. | Javed Yakoob Zaigham Abbas Malik Hassan Mehmood Kanwal Tariq Saima Azhar Saleem Safia Awan Abdul Malik Saeed Hamid Rustam Khan Wasim Jafri | 2017 | Journal of Integrative Medicine2017，15，5： | 0 |
| 7 | Pre-harvest Fruit Losses and Physico-Chemical Analysis of Different Varieties of Pomegranate in Gilgit-Baltistan Pakistan显示文摘 | Azhar Hussain Muhammad Saeed Awan Sartaj Ali Hussain Azhar | 2012 | Journal of Agricultural Science and Technology(B)2012，2，9： | 0 |
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