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| 1 | Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS显示文摘Objective:To identify the bioactive compounds in catechins isolation and its components from green tea GMB-4 clone.Methods:Green tea GMB-4 clones were extracted with distilled water at 90C.Samples were eluted into the column with 10%ethanol.Subsequently,the column was eluted with95%ethanol and evaporated separately.Green tea extract was identified by thin layer chromatography.Catechins were separated by the stationary phase in column chromatography using polyamide with 10%ethanol eluent and 95%ethanol.The results of isolations were analyzed by high performance liquid chromatographic(HPLC)and LCMS/MS.Analysis of catechins by HPLC was done by external standard.Results:Fraction from 10%ethanol showed that four major peaks at retention time of1.663,2.367,2.950 and 4.890,indicated the presence of four catechins components including catechin,epicatechins,gallocatechin and epigallocatechin.Whereas,fraction from 95%ethanol showed two main peaks at retention time of 5.167 and 9.82,which indicated the presence of epigallocatechin gallate(EGCG)and epicatechin gallate(ECG).EGCG(m/z 459),epigallocatechin(m/z 307),ECG(m/z 443),and epicatechin(m/z 291)were isolated and separated successfully using HPLC and LC-MS/MS.Conclusions:The HPLC and LC-MS/MS methods were successfully tuned for the qualitative analysis of green tea extract with EGCG and ECG.Four major catechins were separated and identified by LC-MS/MS,such as EGCG,epigallocatechin,ECG and epicatechin.The result of HPLC analysis showed that EGCG and ECG were main components from catechins isolation of green tea GMB-4 clone. | Erna Susanti Ciptati Retty Ratnawati Aulanni'am Achmad Rudijanto | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，12： | 3 |
| 2 | Isolation of Red Rose Anthocyanin Pigment and Its Application to Inhibit Lipid Oxidation in Yoghurt显示文摘 | E. A. Saati B. W. Simon Yunianta Aulanni'am | 2011 | Journal of Agricultural Science and Technology(A)2011，1，8： | 2 |
| 3 | Mechanism of retinal pericyte migration through Angiopoietin/Tie-2 signaling pathway on diabetic rats显示文摘AIM: To investigate the mechanism of pericyte migration through Angiopoietin-2(Ang-2)/Tie-2 signaling pathway.METHODS: We divided the rats into 5 groups. Each diabetic rat model groups injected with Tie-2 inhibitor, ERK1/2 inhibitor, Akt/PKB inhibitor, and DMSO intravitreal. Retinal digest preparation was done to examine the retinal vasculature including pericyte: endothelial ratio, and morphology of pericyte migration. Tie-2, ERK1/2 and Akt/PKB phosporylation were analyzed by confocal laser scanning microscopy.RESULTS: There was a correlation between pericyte migration with increasing Ang-2(P<0.05). Pericyte number reduced by 40%(1:2.4) after 5 wk diabetes on diabetic rats. The pericyte: endothelial ratio on group with Tie-2 inhibitor were 1:1.8. The same result shows on group with Akt/PKB inhibition. ERK1/2 inhibitor group shows the best results of pericyte: endothelial ratio(1:1.7). Inhibition on Tie-2 receptor decreased the phosphorylation activity of Tie-2, ERK1/2 and Akt/PKB pathway. ERK1/2 inhibition also decreasing the phosphorylation of Tie-2 and Akt/PKB. But on Akt/PKB inhibition, the phosphorylation of Tie-2 and ERK1/2 were relative the same.CONCLUSION: Ang-2 has a role for pericyte migration on diabetic rats through Tie-2 receptor, ERK1/2 and Akt/PKB pathways. ERK1/2 is a dominant pathway based on theability to supress another pathway activity and decreasing pericyte migration on diabetic rats. | Nadia Artha Dewi Aulanni'am Aulanni 'am Hidayat Sujuti Muhammad Aris Widodo Ojoko Wahono Soeatmadji | 2018 | International Journal of Ophthalmology(English edition)2018，11，3： | 1 |
| 4 | The Potency of Sargassum dupficatum Bory Extract on Inflammatory Bowel Disease Therapy in Rattus norvegicus显示文摘 | Aulanni,am Anna Roosdiana Nur Lailatul Rahmah | 2012 | Journal of Life Sciences2012，6，2： | 0 |
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