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| 1 | 液质联用(LC/MS)技术在单克隆抗体(曲妥珠单抗)结构表征上的应用显示文摘目的:应用LC/MS(液质联用)和LC/MSE方法(液相色谱/非数据依赖二级质谱数据采集方法,等频率切换高与低碰撞能量进行质谱数据扫描)对单克隆抗体(曲妥珠单抗,人源化单克隆免疫球蛋白IgG1)的结构进行深度表征。方法:先用LC/MS对曲妥珠单抗完整蛋白及其亚基(轻链和重链)的相对分子质量进行测定;然后用多种酶(胰蛋白酶和AspN)酶解蛋白并用LC/MSE肽图分析法对曲妥珠单抗的氨基酸全序列进行解析和确定,同时对可能发生的翻译后修饰(PTMs)进行定性和定量分析。再通过PNGase F酶来释放抗体上的糖链并应用LC/FLR/MS(液相色谱荧光检测与质谱联用)方法对其糖基化修饰进行结构分析及确认。结果:对曲妥珠单抗的完整蛋白及其亚基(轻链和重链)的相对分子质量测定得到良好的质量准确度,由此可判定特定蛋白修饰在轻链和重链上的分布。通过对曲妥珠单抗酶解后的氨基酸全序列确认,可对其翻译后修饰以及可能的错误氨基酸序列进行解析。该抗体肽图分析的氨基酸序列覆盖率是100%。糖基化分析是通过对游离的N-链接寡糖进行2-AB荧光标记后再用LC/FLR/MS(液相色谱荧光检测与质谱联用)方法来进行的。联合使用这些分析方法,能够有效地对不同批号和不同工艺的抗体药物进行常规质量检测和深度表征,控制药物质量,为患者提供安全可靠的生物治疗药品。结论:结合液质联用(LC/MS和LC/MSE)技术,通过完整蛋白相对分子质量的测量,肽图分析测定氨基酸序列以及翻译后修饰的定性和定量分析,结合游离N-链接寡糖的分析表征,对单克隆抗体曲妥珠单抗进行了全面的结构表征,并建立了一套单克隆抗体结构表征的平台化方案。 | Henry Shion Asish Chakraborty YU Ying-qing 陈熙 孙庆龙 Scott Berger CHEN Wei-bin 宋兰坤 | 2014 | 中国新药杂志2014，23，4： | 13 |
| 2 | Salt tolerance and salinity effects on plants: a review显示文摘 | Asish Kumar Parida Anath Bandhu Das | 2004 | Ecotoxicology and Environmental Safety2004，，3： | 10 |
| 3 | Curcumin as a potential therapeutic candidate for Helicobacter pylori associated diseases显示文摘Curcumin, a yellow pigment and principal polyphenolic Curcuminoid obtained from the turmeric rhizome Curcuma longa, is commonly used as a food-coloring agent. Studies suggest that curcumin has a wide range of beneficial properties e.g., anti-inflammatory, antioxidant, anti-cancer, anti-proliferative, anti-fungal and anti-microbial. These pleiotropic activities prompted several research groups to elucidate the role of curcumin in Helicobacter pylori(H. pylori) infection. This is the first review with this heading where we discussed regarding the role of curcumin as an anti-H. pylori agent along with its potential in other gastrointestinal diseases. Based on several in vitro, early cell culture, animal research and few pre-clinical trials, curcumin projected as a potential therapeutic candidate against H. pylori mediated gastric pathogenesis. This review sheds light on the anti-H. pylori effects of curcumin in different models with meticulous emphasis on its anti-oxidant, anti-inflammatory and anti-carcinogenic effects as well as some critical signaling and effecter molecules. Remarkably, non-toxic molecule curcumin fulfills the characteristics for an ideal chemopreventive agent against H. pylori mediated gastric carcinogenesis but the foremost challenge is to obtain the optimum therapeutic levels of curcumin, due to its low solubility and poor bioavailability. Further, we have discussed about the possibilities for improving its efficacy and bioavailability. Lastly, we concluded with the anticipation that in near future curcumin may be used to develop a therapeutic drug against H. pylori mediated gastric ailments through improved formulation or delivery systems, facilitating its enhanced absorption and cellular uptake. | Avijit Sarkar Ronita De Asish K Mukhopadhyay | 2016 | World Journal of Gastroenterology2016，22，9： | 5 |
| 4 | Novel virulence factor dupA of Helicobacter pylori as an important risk determinant for disease manifestation:An overview显示文摘Helicobacter pylori(H.pylori)is a microaerophilic,Gram-negative,human gastric pathogen found usually in the mucous lining of stomach.It infects more than 50%of the world’s population and leads to gastroduodenal diseases.The outcome of disease depends on mainly three factors:Host genetics,environment and bacterial factors.Among these,bacterial virulence factors such as cagA,vacA are well known for their role in disease outcomes.However,based on the global epidemiological results,none of the bacterial virulence(gene)factors was found to be associated with particular diseases like duodenal ulcer(DU)in all populations.Hence,substantial importance has been provided for research in strain-specific genes outside the cag pathogenicity island,especially genes located within the plasticity regions.dupA found within the plasticity regions was first demonstrated in 2005 and was proposed for duodenal ulcer development and reduced risk of gastric cancer in certain geographical regions.Due to the discrepancies in report from different parts of the world in DU development related to H.pylori virulence factor,dupA became an interesting area of research in elucidating the role of this gene in the disease progression.In this review,we shed light on the detailed information available on the polymorphisms in dupA and their clinical relevance.We have critically appraised several pertinent studies on dupA and discussed their merits and shortcomings.This review also highlights dupA gene as an important biomarker for DU in certain populations. | Jawed Alam Avijit Sarkar Bipul Chandra Karmakar Mou Ganguly Sangita Paul Asish K Mukhopadhyay | 2020 | World Journal of Gastroenterology2020，26，32： | 5 |
| 5 | Salt tolerance and salinity effects on plants: a review显示文摘 | Asish Kumar Parida Anath Bandhu Das | 2004 | Ecotoxicology and Environmental Safety2004，，3： | 4 |
| 6 | Modelling of piping collapses and gully headcut landforms: Evaluating topographic variables from different types of DEM显示文摘The geomorphic studies are extremely dependent on the quality and spatial resolution of digital elevation model(DEM)data.The unique terrain characteristics of a particular landscape are derived from DEM,which are responsible for initiation and development of ephemeral gullies.As the topographic features of an area significantly influences on the erosive power of the water flow,it is an important task the extraction of terrain features from DEM to properly research gully erosion.Alongside,topography is highly correlated with other geo-environmental factors i.e.geology,climate,soil types,vegetation density and floristic composition,runoff generation,which ultimately influences on gully occurrences.Therefore,terrain morphometric attributes derived from DEM data are used in spatial prediction of gully erosion susceptibility(GES)mapping.In this study,remote sensing-Geographic information system(GIS)techniques coupled with machine learning(ML)methods has been used for GES mapping in the parts of Semnan province,Iran.Current research focuses on the comparison of predicted GES result by using three types of DEM i.e.Advanced Land Observation satellite(ALOS),ALOS World 3D-30 m(AW3D30)and Advanced Space borne Thermal Emission and Reflection Radiometer(ASTER)in different resolutions.For further progress of our research work,here we have used thirteen suitable geo-environmental gully erosion conditioning factors(GECFs)based on the multi-collinearity analysis.ML methods of conditional inference forests(Cforest),Cubist model and Elastic net model have been chosen for modelling GES accordingly.Variable’s importance of GECFs was measured through sensitivity analysis and result show that elevation is the most important factor for occurrences of gullies in the three aforementioned ML methods(Cforest=21.4,Cubist=19.65 and Elastic net=17.08),followed by lithology and slope.Validation of the model’s result was performed through area under curve(AUC)and other statistical indices.The validation result of AUC has shown that Cforest is the most appropriate model for predicting the GES assessment in three different DEMs(AUC value of Cforest in ALOS DEM is 0.994,AW3D30 DEM is 0.989 and ASTER DEM is 0.982)used in this study,followed by elastic net and cubist model.The output result of GES maps will be used by decision-makers for sustainable development of degraded land in this study area. | Alireza Arabameri Fatemeh Rezaie Subodh Chandra Pal Artemi Cerda Asish Saha Rabin Chakrabortty Saro Lee | 2021 | Geoscience Frontiers2021，12，6： | 2 |
| 7 | Search for new red phosphors using genetic algorithm-as- sisted combinatorial chemistry 显示文摘 | Chandramouli Kulshreshtha Asish Kumar Sharma Kee-Sun Sohn | 2008 | Journal of Combinatorial Chemistry2008，10，3： | 1 |
| 8 | Salt tolerance and salinity effects on plants: a review显示文摘 | ASISH K P ANATH B D | 2005 | Ecotoxicology and Environmental Safety2005，60，： | 1 |
| 9 | Ultrastructure and adenosine triphosphatase activity of red and white muscle fibers of the caudal region of a fish, Salmo gairdneri显示文摘 | NAG ASISH C | 1972 | Journal Cell Biology1972，55，1： | 1 |
| 10 | Antioxidative Defense Potential to Salinity in the Euhalophyte Salicornia brachiata显示文摘 | Asish Kumar Parida Bhavanath Jha | 2010 | Journal of Plant Growth Regulation2010，，2： | 1 |
| 11 | Enhanced musde fat oxidation and glucose transport by ACRP 30 globular domain:aeetyl-CoA carboxylase inhibition and A/VIP activated protein kinase activation显示文摘 | Toinas E Tsu S Asish K S | 2002 | Proc Nad Acad Sci USA2002，99，16： | 1 |
| 12 | Salt tolerance and salinity effects on plants: a review 显示文摘 | Asish Kumar Parida Anath Bandhu Das | 2005 | Ecotoxicology and Environmental Safety2005，60，3： | 1 |
| 13 | Production of cellulase- Free xylanase by Trichoderma reesei SAF3 显示文摘 | SANJAY K ASISH M PRADEEP KD M | 2006 | Brazilian J Microbiol2006，37，4： | 1 |
| 14 | Grey-based taguchi method for optimization显示文摘 | Saurav Datta Asish Bandyopadhyay Pradip Kumar Pal | 2008 | Int J AdvManuf Technol2008，39，： | 1 |
| 15 | ^13 C-NMR spectra of pentacyclic tfiterpenoids a compilation and some salient features显示文摘 | Shashi B M Asish P K | 1994 | Phytochemistry1994，37，6： | 1 |
| 16 | 13C-NMR spectra of pentacyclic triterpenoids-a compilation and some salient features 显示文摘 | Shashi BM Asish PK | 1994 | Phytochemistry1994，37，6： | 1 |
| 17 | Salt tolerance and salinity effects on plants a review显示文摘 | Asish K P Anath B D | 2005 | Ecotoxicology and Environmental Safety2005，60，： | 1 |
| 18 | ^13C - NMR spectra of pentacylic triterpenoids - a compilation and some salient features 显示文摘 | Shashi B M Asish P K | 1994 | Phytochemistry1994，37，6： | 1 |
| 19 | Suppression of histamine signaling by probiotic lac-B: a possible mechanism of its anti-allergic effect显示文摘 | Shrabanti D Hiroyuki M Asish KD | 2008 | J Pharmacol Sci2008，107，： | 1 |
| 20 | ^13C-NMR spectra of pentacyclic triterpenoids a compilation and some salient features显示文摘 | Shashi B M Asish P K | 1994 | Phytochemistry1994，37，6： | 1 |
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