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| 1 | Ruxolitinib attenuates secondary injury after traumatic spinal cord injury显示文摘Excessive inflammation post-traumatic spinal cord injury(SCI)induces microglial activation,which leads to prolonged neurological dysfunction.However,the mechanism underlying microglial activation-induced neuroinflammation remains poorly understood.Ruxolitinib(RUX),a selective inhibitor of JAK1/2,was recently reported to inhibit inflammatory storms caused by SARS-CoV-2 in the lung.However,its role in disrupting inflammation post-SCI has not been confirmed.In this study,microglia were treated with RUX for 24 hours and then activated with interferon-γfor 6 hours.The results showed that interferon-γ-induced phosphorylation of JAK and STAT in microglia was inhibited,and the mRNA expression levels of pro-inflammatory cytokines tumor necrosis factor-α,interleukin-1β,interleukin-6,and cell proliferation marker Ki67 were reduced.In further in vivo experiments,a mouse model of spinal cord injury was treated intragastrically with RUX for 3 successive days,and the findings suggest that RUX can inhibit microglial proliferation by inhibiting the interferon-γ/JAK/STAT pathway.Moreover,microglia treated with RUX centripetally migrated toward injured foci,remaining limited and compacted within the glial scar,which resulted in axon preservation and less demyelination.Moreover,the protein expression levels of tumor necrosis factor-α,interleukin-1β,and interleukin-6 were reduced.The neuromotor function of SCI mice also recovered.These findings suggest that RUX can inhibit neuroinflammation through inhibiting the interferon-γ/JAK/STAT pathway,thereby reducing secondary injury after SCI and producing neuroprotective effects. | Zhan-Yang Qian Ren-Yi Kong Sheng Zhang Bin-Yu Wang Jie Chang Jiang Cao Chao-Qin Wu Zi-Yan Huang Ao Duan Hai-Jun Li Lei Yang Xiao-Jian Cao | 2022 | Neural Regeneration Research2022，17，9： | 3 |
| 2 | Diagnostic value of fecal occult blood testing for screening colorectal cancer显示文摘DiagnosticvalueoffecaloccultbloodtestingforscreeningcolorectalcancerCHENKun1,JIAODengAo1,ZHENGShu2,ZHOULun2,YUHai2,YUANYaC... | CHEN Kun 1, JIAO Deng Ao 1, ZHENG Shu 2, ZHOU Lun 2, YU Hai 2, YUAN Ya Chang 3, YAO Kai Yan 3, MA Xing Yuan 3 and ZHANG Yang 1 | 1997 | World Journal of Gastroenterology1997，3，3： | 3 |
| 3 | Application of fuzzy multi objective function on reducing groundwater demand for aquaculture in land subsidence areas 显示文摘 | Ao Chang Yang Pao Shan Yu | 2006 | Water Resources Management2006，，20： | 1 |
| 4 | Primary gastric melanoma: A case report with imaging findings and 5-year follow-up显示文摘BACKGROUND Most melanomas identified in the stomach are metastatic;primary gastric melanoma(PGM)is extremely rare,and the relevant studies are relatively scarce.PGM may be incorrectly diagnosed as other gastric malignant tumor types.CASE SUMMARY We describe a rare case of PGM confirmed through long-term clinical observation and pathological diagnosis.A 67-year-old woman presented to our hospital with recurrent chest tightness and chest pain.Digital gastrointestinal radiography revealed a circular shadow in the gastric cardia.Computed tomography(CT)revealed a heterogeneous tumor with uneven enhancement.Enlarged lymph nodes were noted in the lesser curvature of the stomach.On magnetic resonance imaging(MRI),T1-and T2-weighted imaging revealed hyperintensity in and hypointensity in the tumor,respectively,both of which increased substantially after uneven enhancement.Near total gastrectomy was performed,and the tumor was pathologically confirmed to be a gastric melanoma.Because no other possible primary site of malignant melanoma was suspected,a clinical diagnosis of PGM was made.The patient was followed for nearly 5 years,during which she received CT reexamination,but no recurrence or metastasis was observed.CONCLUSION Certain imaging characteristics could be revealed in PGM.Imaging examination can be of great value in preoperative diagnosis,differential diagnosis,and followup of patients with PGM. | Jian Wang Fang Yang Wei-Qun Ao Chang Liu Wen-Ming Zhang Fang-Yi Xu | 2019 | World Journal of Gastroenterology2019，25，44： | 1 |
| 5 | Decarboxylated osteocalcin,a possible drug for type 2 diabetes,triggers glucose uptake in MG63 cells显示文摘BACKGROUND Uncarboxylated osteocalcin(GluOC)has been reported to improve glucose metabolism,prevent type 2 diabetes,and decrease the severity of obesity in mice with type 2 diabetes.GluOC can increase glucose uptake in a variety of cells.Glucose metabolism is the main source of energy for osteoblast proliferation and differentiation.We hypothesized that decarboxylated osteocalcin(dcOC),a kind of GluOC,can increase glucose uptake in MG63 cells(osteoblast-like osteosarcoma cells)and influence their proliferation and differentiation.AIM To investigate the effects of dcOC on glucose uptake in human osteoblast-like osteosarcoma cells and the possible signaling pathways involved.METHODS MG63 cells(human osteoblast-like osteosarcoma cells)were treated with dcOC(0,0.3,3,10,or 30 ng/mL)for 1 and 72 h,and glucose uptake was measured by flow cytometry.The effect of dcOC on cell proliferation was measured with a CCK-8 assay,and alkaline phosphatase(ALP)enzyme activity was measured.PI3K was inhibited with LY294002,and hypoxia-inducible factor 1 alpha(HIF-1α)was silenced with siRNA.Then,GPRC6A(G protein-coupled receptor family C group 6 subtype A),total Akt,phosphorylated Akt,HIF-1α,and glucose transporter 1(GLUT1)levels were measured by Western blot to elucidate the possible pathways by which dcOC modulates glucose uptake.RESULTS The glucose uptake of MG63 cells was significantly increased compared with that of the paired control cells after short-term(1 h)treatment with dcOC at different concentrations(0.3,3,and 10 ng/mL groups,P<0.01;30 ng/mL group,P<0.05).Glucose uptake of MG63 cells was significantly increased compared with that of the paired control cells after long-term(72 h)treatment with dcOC at different concentrations(0.3,3,and 10 ng/mL groups,P<0.01;30 ng/mL group,P<0.05).DcOC triggered Akt phosphorylation in a dose-dependent manner,and the most effective stimulatory concentration of dcOC for short-term(1 h)was 3 ng/mL(P<0.01).LY294002 abolished the dcOC-mediated(1 h)promotion of Akt phosphorylation and glucose uptake without affecting GLUT1 protein expression.Long-term dcOC stimulation triggered Akt phosphorylation and increased the protein levels of HIF-1α,GLUT1,and Runx2 in a dose-dependent manner.Inhibition of HIF-1αwith siRNA abolished the dcOC-mediated glucose uptake and substantially decreased GLUT1 protein expression.DcOC interven-tion promoted cell proliferation in a time-and dose-dependent manner as determined by the CCK-8 assay.Treatment with both 3 ng/mL and 10 ng/mL dcOC affected the ALP activity in MG63 cells after 72 h(P<0.01).CONCLUSION Short-and long-term dcOC treatment can increase glucose uptake and affect proliferation and ALP activity in MG63 cells.This effect may occur through the PI3K/Akt,HIF-1α,and GLUT1 signaling factors. | Shi Jin Xiao-Cen Chang Jing Wen Jing Yang Na Ao Ke-Ying Zhang Lin-Na Suo Jian Du | 2021 | World Journal of Diabetes2021，12，7： | 1 |
| 6 | Synthesis and Biological Evaluation of Pyranone Analogues of Territrem B显示文摘23 compounds containing pyranone pharmacophore of territrem B were designed and synthesized. Some of the analogues showed IC50 values of AChE inhibition at 10-5 mol/L. | Xiang Rui JIANG Lei AO Chang Xin ZHOU Lei Xiang YANG Hai Bo LI Xiu Mei WU Hua BAI Qi Jun ZHANG Yu ZHAO | 2005 | Chinese Chemical Letters2005，16，9： | 0 |
| 7 | Polymer Photovoltaic Cells显示文摘 | Jianhui Hou Chunhe Yang Erjun Zhou Chang He Zhan' ao Tan Youjun He Yongfang Li | 2005 | 复旦学报(自然科学版)2005，44，5： | 0 |
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