3篇
您的检索式：作者名="Angela Gradilone"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Circulating tumor cells isolation:the 'post-EpCAM era'显示文摘Circulating tumor cells(CTCs) represent a submicroscopic fraction detached from a primary tumor and in transit to a secondary site.The prognostic significance of CTCs in metastatic cancer patients was demonstrated for the first time more than ten years ago.To date,it seems clear enough that CTCs are highly heterogeneous and dynamically change their shape.Thus,the inadequacy of epithelial cell adhesion molecule(EpCAM) as universal marker for CTCs detection seems unquestionable and alternative methods able to recognize a broader spectrum of phenotypes are definitely needed.In this review the pleiotropic functions of EpCAM are discussed in detail and the role of the molecule in the biology of CTCs is critically dissected. | Cristina Raimondi Chiara Nicolazzo Angela Gradilone | 2015 | Chinese Journal of Cancer Research2015，27，5： | 4 |
| 2 | Activation of Trpv4 Reduces the Hyperproliferative Phenotype of Cystic Cholangiocytes From an Animal Model of ARPKD显示文摘 | Sergio A. Gradilone Tatyana V. Masyuk Bing Q. Huang Jesus M. Banales Guillermo L. Lehmann Brynn N. Radtke Angela Stroope Anatoliy I. Masyuk Patrick L. Splinter Nicholas F. LaRusso | 2010 | Gastroenterology2010，，1： | 1 |
| 3 | Impact of chronic exposure to bevacizumab on EpCAM-based detection of circulating tumor cells显示文摘Background:Circulating tumor cells(CTCs) are often undetected through the immunomagnetic epithelial cell adhesion molecule(EpCAM)-based CellSearch~ System in breast and colorectal cancer(CRC)patients treated with bevacizumab(BEV),where low CTC numbers have been reported even in patients with evidence of progression of disease.To date,the reasons for this discrepancy have not been clarified.This study was carried out to investigate the molecular and phenotypic changes in CRC cells after chronic exposure to BEV in vitro.Methods:The human CRC cell line WiDr was exposed to a clinically relevant dose of BEV for 3 months in vitro.The expression of epithelial and mesenchymal markers and EpCAM isoforms was determined by western blotting and immunofluorescence.To evaluate the impact of EpCAM variant isoforms expression on CTC enumeration by CellSearch~,untreated and treated colon cancer cells were spiked into 7.5 mL of blood from a healthy donor and enumerated by CellSearch~.Results:Chronic exposure of CRC cell line to BEV induced decreased expression of EpCAM 40 kDa isoform and increased expression EpCAM 42 kDa isoform,together with a decreased expression of cytokeratins(CK),while no evidence of epithelial to mesenchymal transition(EMT) in treated cells was observed.The recovery rate of cells through CellSearch~ was gradually reduced in course of treatment with BEV,being 84%,70%and 40%at 1,2 and 3 months,respectively.Conclusions:We hypothesize that BEV may prevent CellSearch~ from capturing CTCs through altering EpCAM isoforms. | Chiara Nicolazzo Isabella Massimi Lavinia V.Lotti Simone Vespa Cristina Raimondi Fabio Maria Pulcinelli Angela Gradilone Paola Gazzaniga | 2015 | Chinese Journal of Cancer Research2015，27，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员