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| 1 | Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide显示文摘Endothelial cell death due to increased reactive oxygen species(ROS) may contribute to the initial endothelial injury,which promotes atherosclerotic lesion formation.Piper sarmentosum(PS),a natural product,has been shown to have an antioxidant property,which is hypothesized to inhibit production of ROS and prevent cell injury.Thus,the present study was designed to determine the effects of PS on the hydrogen peroxide(H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells(HUVECs).In this experiment,HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation(LSGS).HUVECs were treated with various concentrations of H2O2(0-1000 μmol/L) and it was observed that 180 μmol/L H2O2 reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay.Using the above concentration as the positive control,the H2O2-induced HUVECs were concomitantly treated with various concentrations(100,150,250 and 300 μg/ml) of three different extracts(aqueous,methanol and hexane) of PS.Malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GPX) levels showed a significant increase(P<0.05) in HUVECs compared to the negative control.However,PS extracts showed a protective effect on HUVECs from H2O2-induced cell apoptosis with a significant reduction in MDA,SOD,CAT and GPX levels(P<0.05).Furthermore,PS had exhibited ferric reducing antioxidant power with its high phenolic content.Hence,it was concluded that PS plays a beneficial role in reducing oxidative stress in H2O2-induced HUVECs. | Abdul Hamid HAFIZAH Zakaria ZAITON Amom ZULKHAIRI Adenan MOHD ILHAM Megat Mohd Nordin NOR ANITA Abdullah Mahdy ZALEHA | 2010 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2010，11，5： | 13 |
| 2 | Killing of Staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity显示文摘OBJECTIVE:This study investigated the effects of allylpyrocatechol(APC),the major component in ethanolic extract of Piper betle,on key oxidative stress resistance enzymes important for the survival of Staphylococcus aureus,a major pathogen in the human host.METHODS:Effects of APC on expressions of genes encoding catalase(katA),superoxide dismutases(SODs),including sod A and sod M,and alkyl hydroperoxide reductase(ahpC) in S.aureus were quantitated by RT-qPCR in reference to gyrA and 16S rRNA.Corresponding activities of the enzymes were also investigated.The Livak analysis was performed for verification of gene-fold expression data.Effects of APC on intracellular and extracellular reactive oxygen species(ROS) levels were determined using the nitroblue tetrazolium(NBT) reduction assay.RESULTS:APC-treated S.aureus cells had higher sodA and sodM transcripts at 1.5-fold and 0.7-fold expressions respectively with corresponding increase in total SOD activity of 12.24 U/mL compared to untreated cells,10.85 U/mL(P<0.05).Expression of ahpC was highest in APC-treated cells with 5.5-fold increased expression compared to untreated cells(P<0.05).Correspondingly,ahpC activity was higher in APC-treated cells at 0.672(A_(310nm)) compared to untreated cells which was 0.394(A_(310nm)).In contrast,katA expression was 1.48-fold and 0.33-fold lower respectively relative to gyrA and 16 S rRNA.Further,APC-treated cells showed decreased catalase activity of 1.8×10^(-4)(U/L or umol/(minL)) compared to untreated cells,which was 4.8 × 10^(-4)U/L(P<0.05).Absorbance readings(A_(575nm)) for the NBT reduction assay were 0.709 and 0.695 respectively for untreated and treated cells,which indicated the presence of ROS.APC-treated S.aureus cells had lower ROS levels both extracellulariy and intracellularly,but larger amounts remained intracellularly compared to extracellular levels with absorbances of 0.457 and 0.137respectively(P<0.05).CONCLUSION:APC induced expressions of both sodA and sodM,resulting in increased total SOD activity in S.aureus.Higher sodA expression indicated stress induced intracellularly involving O_2^-,presumably leading to higher intracellular pools of H_2O_2.A concommittant decrease in katA expression and catalase activity possibly induced ahpC expression,which was increased the highest in APC-treated cells.Our findings suggest that in the absence of catalase,cells are propelled to seek an alternate pathway involving ahpC to reduce stress invoked by 0_2^- and H_2O_2.Although APC reduced levels of ROS,significant amounts eluded its antioxidative action and remained intracellularly,which adds to oxidative stress in treated cells. | Roslinah Mohamad Hussain Noor Faradilla Abdullah Zulkhairi Amom | 2016 | Journal of Integrative Medicine2016，14，6： | 2 |
| 3 | Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis显示文摘 | Amom Z Zakaria Z Mohamed J | 2008 | J Clin Biochem Nutr2008，43，2： | 1 |
| 4 | Preparation of Poly ( 1 - Lactic Acid) Microencapsulated Vitamin E 显示文摘 | Preeyapom C Amom C Paweena T | 2013 | Energy Procedia2013，34，： | 1 |
| 5 | Antiobesity effect of Tamarindus indica L. pulp aqueous extract in high-fat diet-induced obese rats显示文摘 | Khairunnuur Azman Zulkhairi Amom Azrina Azlan Norhaizan Esa Rasadah Ali Zamree Shah Khairul Kadir | 2012 | Journal of Natural Medicines2012，，2： | 1 |
| 6 | Co-existence between oral lesions and opportunistic systemie diseases among HIV- infected subjects in Thailand显示文摘 | Khaehornsakdi S Amom R Sutep J | 2002 | J Oral Pathol Med2002，31，： | 1 |
| 7 | Lipid lowering effect of antioxidant alpha-lipoic Acid in experimental atheroscle- rosis显示文摘 | Amom Z Zakaria Z Mohamed J | 2008 | J Clin Bioehem Nutr2008，43，2： | 1 |
| 8 | Lipid lowering effect of antiox- idant alpha-lipoic Acid in experimental atheroselerosis 显示文摘 | Amom Z Zakaria Z Mohamed J | 2008 | J Clin Bio- chem Nut r2008，43，2： | 1 |
| 9 | Antioxidant activity of white rice, brown rice and germinated brown rice (in vivo and in vitro) and the effects on lipid peroxidation and liver enzymes in hyperiipidaemic rabbits显示文摘 | Mohd Esa N Abdul Kadir K K Amom Z et ai | | Food Ch0，，： | 1 |
| 10 | Improving the lipid profile in hypercholesterolemia-induced rabbit by supplementation of germinated brown rice显示文摘 | S E N Mohd K K Abdul Kadir Z Amom A Azlan | 2011 | Journal of Agricultural and Food Chemistry2011，59，79： | 1 |
| 11 | Antioxidant activity of white rice,brown rice and germinated brown rice(in vivo and in vitro)and the effects on lipid peroxidation and liver enzymes in hyperlipidaemic rabbits显示文摘 | Norhaizan Mohd Esa Khairul Kamilah Abdul Kadir Zulkhairi Amom | 2013 | Food Chemistry2013，141，2： | 1 |
| 12 | Changes in immunoglobulin levels following adenoideetomy and ton-sillectomy显示文摘 | Amoms Sebastia LI Ferrer Ramirez MJ lopez Molla C et a1 | 2004 | Aeta Otorrinolaringol Esp2004，55，： | 1 |
| 13 | Lipid lowering effect of antioxidant alpha-lipoic Acid in experimental atherosclerosis显示文摘 | Amom Z Zakaria Z Mohamed J | 2008 | J Clin Biochem Nutr2008，43，2： | 1 |
| 14 | State of the art of biogas technology in Austria 显示文摘 | Hopfenr S K Amom T Bodiroza V | 2006 | Landtechnik2006，61，1： | 1 |
| 15 | Restoraton ecology of riverine wetlands (Ⅰ):A scientific base显示文摘 | Henry C P Amoms C | | 0，，06： | 1 |
| 16 | Tem peralure programmed desorplion study on the mecbarism of SO2 oxi dation by activated carbon and activated carbon fibers 显示文摘 | Raymundo - Pinero E Cazorla - Amoms D Linares - Solano A | 2001 | Carbon2001，39，2： | 1 |
| 17 | Muhimode Heat Transfer in Cyclic Flow Reversal Combustion in A Porous Medium显示文摘 | Sumrerng Jugjai Amom Somjetlertcharoen | 1999 | Int J Energy Res1999，23，： | 1 |
| 18 | Screening and Analysis of Methanolic Leaf Extract of Psorospermum febrifugum(SPACH)显示文摘Phytochemical components have been reported for various plants but very little information on Psorospermum febrifugum(SPACH).The presence of biocidal activity makes the spach of potential interest for the control of micro-organisms.Methanolic extract of the leaves of spach shows the various constituents(alkaloids,flavonoids,terpenoids,tannins,phenols,and steroids).Further investigation revealed phytoconstituents of methanolic leaf extract using gas chromatography-spectrophotometric techniques(GC-MS).Result of GC-MS analysis revealed the presence of eight(8)botanical pesticides with valuable biological activities.The GC-MS results revealed that eight(8)biocidal activities were present in spach namely:1,2,3,4-tetrachloronaphtalene,Permetrin-a,permetrin-b,cyfluthrin-b,cypermethrin-a,cypermethrin-c,and flumethrin-b.The result clearly shows that Psorospermum febrifugum hold phytocomponents species of botanical interest that could still be exploited. | Elaigwu Matthew Hyacinth Ocheigwu Apeh Oluma Terrumun Amom Tor-Anyiin | 2022 | Journal of Botanical Research2022，4，2： | 0 |
| 19 | Sedative, Cognitive Impairment and Anxiolytic Effects of Acute Mitragyna Speciosa in Rodents显示文摘 | Mohamad Aris Mohd Moklas Noor Azuin Suliman Che Norma Mat Taib Mohamad Taufik HidayatBaharuldin Sharida Fakurazi Fatin Nadzirah Zakaria Muhammad Khairulasraf Muhamamd Yusof Muhammad Farhan Adzhar Mohd Saifuddin Md Rasul Abdah Md Akim Zulkhairi Amom | 2013 | Journal of US-China Medical Science2013，10，1： | 0 |
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