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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Distinct structural and ionotropic roles of NMDA receptors in controlling spine and syn- apse stability显示文摘 | Alvarez VA Ridenour DA Sabatini BL | 2007 | J Neurosci2007，27，： | 1 |
| 3 | A hippocampal NR2Bdeficit can mimic age-related changes in long-term potentiation andspatial learning in the Fischer 344 rat显示文摘 | Clayton DA Mesches MH Alvarez E | 2002 | Neuroscience2002，22，9： | 1 |
| 4 | Friction stir spot welding of AAI050 Al alloy and hot stamped boron steel (22MnB5) 显示文摘 | Da Silva A A M Aldanondo E Alvarez P | 2010 | Science and Technology of Welding and Joining2010，15，8： | 1 |
| 5 | Material flow and mechanical behaviour of dissimilar AA2024-T3 and AA7075-T6 aluminium alloys friction stir welds显示文摘 | A.A.M. da Silva E. Arruti G. Janeiro E. Aldanondo P. Alvarez A. Echeverria | 2010 | Materials and Design2010，，4： | 1 |
| 6 | A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat 显示文摘 | Clayton DA Mesches MH Alvarez E | 2002 | J Neurosci2002，22，9： | 1 |
| 7 | A hippocampal NR2B deficit can mimic age-related changes in long-term potenti- ation and spatial learning in the Fischer 344 rat显示文摘 | Clayton DA Mesches MH Alvarez E | 2002 | Neuro- science2002，22，9： | 1 |
| 8 | Depression in Par- kinson' s disease : A double - blind, randomized, placebo - con trolled pilot study of omega- 3 fatty acid supplementation显示文摘 | da Silva TM Munhoz RP Alvarez C | 2008 | J Affective Disorders2008，111，： | 1 |
| 9 | A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 544 rat显示文摘 | Clayton DA Mesches MH Alvarez E | 2002 | Neuroscience2002，22，： | 1 |
| 10 | A hippocampal NR2B deficient can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat显示文摘 | Clayton DA Mesches MH Alvarez E | 2002 | J Neurosci2002，22，9： | 1 |
| 11 | Classification of hematite types in iron ores through circularly polarized light microscopy and image analysis显示文摘 | Ot(lvio da Fonseca Martins Comes Julio Cesar Alvarez Iglesias Sid- net Paciornik | 2013 | Minerals Engineering2013，52，： | 1 |
| 12 | Hippocampal NR2B Deficit Can Mimic Age-Related Changes in Long-Term Potentiation and Spatial Learning in the Fischer 344 Rat显示文摘 | Clayton DA Michael MH Alvarez E | 2002 | Neuroscience2002，22，9： | 1 |
| 13 | Disruption of CD8 +Treg activity results in expansion of T follicular helper cells andenhanced antitumor immunity 显示文摘 | Alvarez Arias DA Kim HJ Zhou P | 2014 | Cancer Immunol Res2014，2，3： | 1 |
| 14 | The interaction between managerial and technological capabilities as a determinant of company performance: an empirical study of biotecb firms 显示文摘 | Welter C Bosse DA Alvarez SA | 2013 | International Journal of Management2013，30，1： | 1 |
| 15 | A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat显示文摘 | Clayton DA Mesches MH Alvarez E | 2002 | J Neurosci2002，22，9： | 1 |
| 16 | A hippocampal NR2B deficit can mimic age related changes in long-term potentiation and spatial learning in the fischer 344 rat显示文摘 | Clayton DA Mesches MH Alvarez E | 2002 | J Neurosci2002，22，9： | 1 |
| 17 | For the long run: maintaining germinal niches in the adult braln显示文摘 | ALVAREZ B LIM DA | 2004 | Neuron2004，41，： | 1 |
| 18 | Molecular phylogeny and proposal of two new species of the emerging path- ogenic fungus Saksenaea 显示文摘 | Alvarez E Garcia-Hermoso D Sutton DA | 2010 | J Clin Microbiol2010，48，12： | 1 |
| 19 | The Penicillium echinulatum secretome on sugar cane bagasse显示文摘 | Ribeim DA Cota J Alvarez TM | 2012 | PLoS One2012，7，12： | 1 |
| 20 | Unexpected role of clathrin adaptor AP-1 in MHC-dependent positive selection of T cells显示文摘 | Alvarez DA McCarty N Lu L | 2010 | Proc Natl Acad Sci U S A2010，107，6： | 1 |
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