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| 1 | Regulatory mechanisms for abnormal expression of the human breast cancer specific gene 1 in breast cancer cells显示文摘Breast cancer-specific gene 1 (BCSG1), also referred as synuclein γ, was originally iso-lated from a human breast cancer cDNA library and the protein is mainly localized to presynaptic terminals in the nervous system. BCSG1 is not expressed in normal or benign breast lesions, but expressed at an extremely high level in the vast majority of the advanced staged breast carcinomas and ovarian carcinomas. Overexpression of BCSG1 in cancer cells led to significant increase in cell proliferation, motility and invasiveness, and metastasis. To elucidate the molecular mechanism and regulation for abnormal transcription of BCSG1, a variety of BCSG1 promoter luciferase reporters were constructed including 3′ end deleted sequences, Sp1 deleted, and activator protein-1 (AP1) domains mutated. Transient transfection assay was used to detect the transcriptional activation of BCSG1 promoters. Results showed that the Sp1 sequence in 5′-flanking region was involved in the basal transcriptional activities of BCSG1 without cell-type specificity. In comparison to pGL3-1249, the reporter activities of pGL3-1553 in BCSG1-negative MCF-7 cells and pGL3-1759 in HepG2 cells were notably decreased. Mutations at AP1 sites in BCSG1 intron 1 significantly reduced the promoter ac-tivity in all cell lines. Transcription factors, c-jun, c-fos and cyclin AMP-responsive element binding (CREB) protein, could markedly enhance the promoter activities. Thus, our results suggest that the abnormal expression of BCSG1 in breast cancer cells is likely regulated by multiple mechanisms. The 5′ flanking region of BCSG1 provides the basal transcriptional activity without cell type specificity. A critical promoter element involved in abnormal expression of BCSG1 presents in the first exon. The cell type specificity of BCSG1 transcription is probably affected through intronic cis-regulatory se-quences. AP1 domains in the first intron play an important role in control of BCSG1 transcription. | LU Aiping1, LI Qing1, LIU Jingwen2 1. Department of Pathology, Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100036, China 2. VA Palo alto Health Care System, Palo Alto, California, CA 94304, USA | 2006 | Science China(Life Sciences)2006，49，4： | 8 |
| 2 | Synthesis,Contact Printing,and Device Characterization of Ni-Catalyzed,Crystalline InAs Nanowires显示文摘InAs nanowires have been actively explored as the channel material for high performance transistors owing to their high electron mobility and ease of ohmic metal contact formation.The catalytic growth of nonepitaxial InAs nanowires,however,has often relied on the use of Au colloids which is non-CMOS compatible.Here,we demonstrate the successful synthesis of crystalline InAs nanowires with high yield and tunable diameters by using Ni nanoparticles as the catalyst material on amorphous SiO_(2) substrates.The nanowires show superb electrical properties with field-effect electron mobility~2700 cm^(2)/Vs and ION/IOFF>10^(3).The uniformity and purity of the grown InAs nanowires are further demonstrated by large-scale assembly of parallel arrays of nanowires on substrates via the contact printing process that enables high performance,“printable”transistors,capable of delivering 510 mA ON currents(~400 nanowires). | Alexandra C.Ford Johnny C.Ho Zhiyong Fan Onur Ergen Virginia Altoe Shaul Aloni Haleh Razavi Ali Javey | 2008 | Nano Research2008，1，1： | 3 |
| 3 | Diagnostic value of whole-body MRI with diffusion-weighted sequence for detection of peritoneal metastases in colorectal malignancy显示文摘Objective:To assess the diagnostic accuracy of whole-body MRI using diffusion-weighted sequence(WB-DWI)to determine the peritoneal cancer index(PCI)in correlation with surgical and histopathological findings.Methods:Twenty-seven patients underwent preoperative WB-MRI,followed by cytoreductive surgery for primary tumors of the appendix(n=15),colorectum(n=12),and associated peritoneal disease.A total of 351 regions were retrospectively reviewed.The sensitivity,specificity,and accuracy were calculated at 13 anatomical sites.The WB-DWI PCI and PCI type were compared with surgical and histopathological findings.Results:No statistical difference was found between the WB-DWI PCI and surgical PCI(P=0.574).WB-DWI correctly predicted the PCI type in 24 of 27 patients with high accuracy(88.9%),including 10 of 10 patients with small-volume tumor,12 of 14 with moderate-volume tumor,and 2 of 3 with large-volume tumor.WB-DWI correctly depicted tumors in 163 of 203 regions,with 40false-negative and 23 false-positive regions.The overall sensitivity,specificity,and accuracy of WB-DWI for the detection of peritoneal tumors were 80.3%,84.5%,and 82.1%,respectively.For lesions<0.5 cm in diameter,WB-DWI demonstrated good sensitivity(69.4%).Conclusions:WB-DWI accurately predicted PCI before surgery in patients undergoing evaluation for cytoreductive surgery. | Huan Zhang Weixing Dai Caixia Fu Xu Yan Alto Stemmer Tong Tong Guoxiang Cai | 2018 | Cancer Biology & Medicine2018，15，2： | 3 |
| 4 | 液相色谱-紫外-核磁共振-质谱:多信息分离方法(英文)显示文摘The availability of liquid chromatography ultraviolet nuclear magnetic resonance mass spectrometry (LC UV NMR MS)integrated systems is providing greater information content and confidence for a variety of sample types, such asdrug metabolites, natural products, impurities, and degradants. A wide range of experimental strategies can be pur sued,highlighting the flexibility and performance possible with this multihyphenated technique. | Iain Green (Varian, Inc., 3120 Hansen Way, Palo Alto, CA 94303, U.S.A.) | 2003 | 波谱学杂志2003，20，4： | 3 |
| 5 | Characterization of focal liver lesions by ADC measurements using a respiratory triggered diffusion-weighted single-shot echo-planar MR imaging technique显示文摘 | Melanie Bruegel Konstantin Holzapfel Jochen Gaa Klaus Woertler Simone Waldt Berthold Kiefer Alto Stemmer Carl Ganter Ernst J. Rummeny | 2008 | European Radiology2008，，： | 2 |
| 6 | The Italian version of the obsess- ive compulsive inventory:its psychometric properties on communi- ty and clinical samples 显示文摘 | Sica C Ghisi M Altoe G | 2009 | J Anxiety Disord2009，23，： | 1 |
| 7 | The heterogeneity of myocardial sympathetic innervation in normal subjects: an assessment by idodine- 123 metaiodobenzylguanidine scintigraphy显示文摘 | D'Alto M Maurea S Basso A | 1998 | Cardidogia1998，43，11： | 1 |
| 8 | Clinical implications of growth factors in flexor tendon wound healing显示文摘 | Hsu C Alto P Chang J | 2004 | Hand Surg2004，4，： | 1 |
| 9 | Spinal cord injuries due to diving accidents 显示文摘 | Alto S D'Andrea M Werhagen L | 2005 | Spinal Cord2005，2，： | 1 |
| 10 | Anxiety and stress in mothers and fathers in the 24 h after their child's surgery 显示文摘 | Scrimin S Haynes M Altoe G | 2009 | Child Care Health Dev2009，35，2： | 1 |
| 11 | Aortic and left ventricular remodeling in patients with bicuspid aortic valve without significant valvular dysfunction: A prospective study显示文摘 | Giuseppe Santarpia Giancarlo Scognamiglio Giovanni Di Salvo Michele D’Alto Berardo Sarubbi Emanuele Romeo Ciro Indolfi Maurizio Cotrufo Raffaele Calabrò | 2011 | International Journal of Cardiology2011，，3： | 1 |
| 12 | Effects of raised CO2 on potential CH4 production and oxidation in, and CH4 emission from, a boreal mire显示文摘 | Saarnio S Alto J Martikainen P J | 1998 | Clinical & Experimental Allergy1998，86，2： | 1 |
| 13 | Identification of a bacterial type III effector family with G protein mimicry functions显示文摘 | Alto NM Shao F Lazar CS | 2006 | Cell2006，124，1： | 1 |
| 14 | Macular edema and visual outcome following cataract surgery in patients with diabetic retinopathy and controls 显示文摘 | Eriksson U Alto A Bjarnhall G | 2011 | Graefe''s Arch Clin Exp Ophthalmol2011，249，3： | 1 |
| 15 | Spinal cord injuries due to diving accidents显示文摘 | Alto S D'Andrea M Werhagen L | 2005 | Spinal Cord2005，43，2： | 1 |
| 16 | Size alters susceptibility of vectors to dengue virus infection and dissemination显示文摘 | Alto BW Reiskind MH Lounibos LP | 2008 | Am J Trop Med Hyg2008，79，5： | 1 |
| 17 | The type Ⅲ effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotie signaling pathways显示文摘 | Alto NM Weflen AW Rardin MJ | 2007 | J Cell Biol2007，178，7： | 1 |
| 18 | Myocardial cation contents during induction of calcium paradox显示文摘 | Alto A Dhalla N S | 1979 | Am J Physiol1979，237，： | 1 |
| 19 | Laboratory markers of systemic inflammation as predictors of bloodstream infection in acutely ill patients admitted to hospital in medical emergency显示文摘 | Alto H Takala A Kautiainen H et at | 2004 | Eur Clin Microbiol Infect Dis2004，23，9： | 1 |
| 20 | Use of microbial data for hazard analysis and critical control point-food and drug administration perspective显示文摘 | John E Kvenberg Darrell J Schw alto | 2000 | Journal of Food Protection2000，63，6： | 1 |
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