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| 1 | Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa显示文摘Background: Lycopene(LYC) is a natural carotenoid with powerful reactive oxygen species(ROS) scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate(Fe AA). Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment(0.25, 0.5, 1 or 2 mmol/L) in the presence or absence of Fe AA(150 μmol/L Fe SO_4 and 750 μmol/L ascorbic acid) during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the Sperm Vision?computer-aided sperm analysis(CASA) system. Cell viability was examined with the metabolic activity(MTT) assay,ROS generation was quantified via luminometry and the nitroblue-tetrazolium(NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx) as well as the concentrations of glutathione(GSH) and malondialdehyde(MDA).Results: FeA A treatment led to a reduced spermatozoa motility(P < 0.001), viability(P < 0.001) and a decline of the antioxidant capacity of spermatozoa(P < 0.001) but increased the ROS generation(P < 0.001), superoxide production(P < 0.001) and lipid peroxidation(P < 0.001). LYC administration resulted in a preservation of the spermatozoa motion parameters(P < 0.001), mitochondrial activity(P < 0.001) and antioxidant characteristics(P < 0.001 with respect to SOD;P < 0.01 in relation to CAT; P < 0.05 as for GPx and GSH) with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.Conclusions: Our results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells. | Eva Tvrdá Anton Kovácik Eva Tusimová Dusan Paál Alica Mackovich Jakhongir Alimov Norbert Lukác | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 3 |
| 2 | Effect of the heat of reaction on the development of thermochemical instability显示文摘 | ALIMOV D T EDVABNYI IV | 1985 | Fizika Goreniyai Vzryva1985，21，5： | 1 |
| 3 | Combined LOH/ CGH analysis proves the existence of interstitial 3p deletions in renal cell carcinoma显示文摘 | Alimov A Kost-Alimova M Liu J | 2000 | Oncogene2000，19，11： | 1 |
| 4 | Some general regularities of the process of filtration in bivalved molluscs显示文摘 | Alimov A F | 1969 | Zhurnal Obshchei Biologii1969，，30： | 1 |
| 5 | Hydrogen Isotope Retention in Plasma-Facing Materials: Review of Recent Experimental Results 显示文摘 | Alimov V K Roth J | 2007 | PhysScr2007，128，： | 1 |
| 6 | Product Market Competition and the Value of Corporate Cash:Evidence from Trade Liberalization显示文摘 | Alimov A | 2014 | Journal of Corporate Finance2014，25，1： | 1 |
| 7 | Response of aquatic communities to anthropogenic stress: a comparative study of Neva Bay and the eastern Gulf of Finland 显示文摘 | Telesh I V Alimov A F Golubkov S M | 1999 | Hydrobiologia1999，393，： | 1 |
| 8 | Somatic mutation and homozygous deletion of PTEN/MMACl gene of 10q23 in renal cell carcinoma显示文摘 | LI Chun-de GIZATULLIN R | 1999 | Anticancer Res1999，19，5： | 1 |
| 9 | Trapping of permeating deuterium in defect induced at the rear side of tungsten samples显示文摘 | Tyburska B Alimov V Kh Ogorodnikova O V | 2011 | Journal of Nuclear Materials2011，415，： | 1 |
| 10 | Legal Institutions And Capital Raising Activities Of Newly Public Firms显示文摘 | Alimov A Hertzel M G | 2012 | German LJ2012，，4： | 1 |
| 11 | Response of aquatic communities to anthropogenic stress: a comparative study of Neva Bay and the eastern Gulf of Finland显示文摘 | Telesh I V Alimov A F Golubkov S M | 1999 | Hydrobiologia1999，393，： | 1 |
| 12 | Combined LOH/CGH analysis proves the existence of interstitial 3p deletions in renal cell carcinoma显示文摘 | Alimov A Kost M Liu J | 2000 | Oncogene2000，19，9： | 1 |
| 13 | Feature of the multifractal structure of small-scale ionospheric turbulence during the solar eclipse of august 1,2008显示文摘 | V A Alimov F I Vybornov A V Rakhlin | 2009 | Radiophysics and Quantum Electronics2009，52，4： | 1 |
| 14 | Re- sponse of aquatic communities to anthropogenic stress:a comparative study of Neva Bay and the east ern Gulf of Finland 显示文摘 | Telesh I V Alimov A F Golubkov S M | 1999 | Hydrobiologia1999，，393： | 1 |
| 15 | Product Market Competition and the Value of Corporate Cash: Evidence from Trade Liberalization显示文摘 | Alimov A | 2014 | Joumal of Corporate Finance2014，25，11： | 1 |
| 16 | Cell-free synthesis and affinity isolation of proteins on a nanomole scale 显示文摘 | Alimov AP Khmelnitsky A Simonenko PN | 2000 | Biotechniques2000，28，2： | 1 |
| 17 | Alternative Perfusion Technique Without Axillary Artery Cannulation During Combined Aortic Root and Total Arch Replacement显示文摘 | Abdallah K. Alameddine V.K. Alimov J.E. Flack | 2013 | J Card Surg2013，，4： | 1 |
| 18 | Evolution of CD4 from bulk boronized graphite and B4C and boron-doped graphite显示文摘 | ALIMOV V K ZALAVUTDINOV R K | 1994 | J Nucl Mater1994，，： | 1 |
| 19 | Mutation analysis of MEN1,HRPT2,CASR,CDKN1B,and AIP genes in primary hyperparathyroidism patients with features of genetic predisposition显示文摘 | Vierimaa O Villablanca A Alimov A | 2009 | J Endocrinol Invest2009，32，6： | 1 |
| 20 | Investigation of Nda+ ions spectroscopic and laser properties in SrF2 fluoride singlecrystal显示文摘 | Alimov O K Basiev T T Doroshenko M E | 2012 | Opt Mater2012，34，5： | 1 |
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