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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Role of mi RNAs and their potential to be useful as diagnostic and prognostic biomarkers in gastric cancer显示文摘Alterations in epigenetic control of gene expression play an important role in many diseases, including gastric cancer. Many studies have identified a large number of upregulated oncogenic mi RNAs and downregulated tumour-suppressor mi RNAs in this type of cancer. In this review, we provide an overview of the role of mi RNAs, pointing to their potential to be useful as diagnostic and/or prognostic biomarkers in gastric cancer. Moreover, we discuss the influence of polymorphisms and epigenetic modifications on mi RNA activity. | Kelly Cristina da Silva Oliveira Taíssa Maíra Thomaz Araújo Camila Inagaki Albuquerque Gabriela Alcantara Barata Carolina Oliveira Gigek Mariana Ferreira Leal Fernanda Wisnieski Fernando Augusto Rodrigues Mello Junior André Salim Khayat Paulo Pimentel de Assumpcao Rommel Mário Rodriguez Burbano Marília Cardoso Smith Danielle Queiroz Calcagno | 2016 | World Journal of Gastroenterology2016，22，35： | 11 |
| 3 | TLR2 and TLR4 polymorphisms influence m RNA and protein expression in colorectal cancer显示文摘AIM: To evaluate the effect of promoter region polymorphisms of toll-like receptor(TLR)2-196 to-174 del and TLR4-1607T/C(rs10759932) on m RNA and protein expression in tumor tissue and of TLR4+896A/G(rs4986790) on colorectal cancer(CRC) risk.METHODS: The TLR2-196 to-174 del polymorphism was investigated using allele-specific polymerase chain reaction(PCR) and the TLR4-1607T/C and TLR4+896A/G by PCR-restriction fragment length p o l y m o r p h i s m( R F L P). W e g e n o t y p e d 4 3 4 D N A samples from 194 CRC patients and 240 healthy individuals. The m RNA relative quantification(RQ) was performed in 40 tumor tissue samples by quantitative PCR Taq Man assay, using specific probes for TLR2 and TLR4 genes, and ACTB and GAPDH reference geneswere used as endogenous controls. Protein expression was analyzed by immunohistochemistry with specific primary antibodies.RESULTS: No association was found for TLR4-1607T/C and TLR4+896A/G by three statistical models(logadditive, dominant and recessive). However, based on dominant and log-additive models, the polymorphic variant TLR2-196 to-174 del was associated with increased CRC risk [dominant: odds ratio(OR) = 1.72, 95%CI: 1.03-2.89; P = 0.038 and log-additive: OR =1.59, 95%CI: 1.02-2.48; P = 0.039]. TLR2 m RNA expression was increased in tumor tissue(RQ = 2.36) when compared to adjacent normal tissue(RQ = 1; P < 0.0001), whereas the TLR4 m RNA showed a basal expression(RQ = 0.74 vs RQ = 1, P = 0.452). Immunohistochemistry analysis of TLR2 and TLR4 protein expression was concordant with the findings of m RNA expression. In addition, the TLR2-196 to-174 del variant carriers showed m RNA relative expression 2.19 times higher than wild-genotype carriers. The TLR2 protein expression was also higher for the TLR2-196 to-174 del variant carriers [117 ± 10 arbitrary unit(a.u.) vs 95 ± 4 a.u., P = 0.03]. However, for the TLR4-1607T/C polymorphism no significant difference was found for both m RNA(P = 0.56) and protein expression(P = 0.26).CONCLUSION: Our findings suggest that TLR2-196 to-174 del polymorphism increases TLR2 m RNA expression and is associated with higher CRC risk, indicating an important role in CRC genetic susceptibility. | Marcela Alcantara Proenca Juliana Garcia de Oliveira Aline Cristina Targa Cadamuro Maysa Succi Joao Gomes Netinho Eny Maria Goloni-Bertolo érika Cristina Pavarino Ana Elizabete Silva | 2015 | World Journal of Gastroenterology2015，21，25： | 9 |
| 4 | Costimulation of resting B lymphocytes alters the IL-4-activated IRS2 signaling pathway in a STAT6 independent manner: implications for cell survival and proliferation显示文摘IL-4 is an important B cell survival and growth factor. IL-4 induced the tyrosine phosphorylation of IRS2 in resting B lymphocytes and in LPS- or CD40L-activated blasts. Phosphorylated IRS2 coprecipitated with the p85 subunit of PI 3’ kinase in both resting and activated cells. By contrast, association of phosphorylated IRS2 with GRB2 was not detected in resting B cells after IL-4 treatment although both proteins were expressed. However, IL-4 induced association of IRS2 with GRB2 in B cell blasts. The pattern of IL-4- induced recruitment of p85 and GRB2 to IRS2 observed in B cells derived from STAT6 null mice was identical to that observed for normal mice. While IL-4 alone does not induce activation of MEK, a MEKI inhibitor suppressed the IL-4-induced proliferative response of LPS-activated B cell blasts. These results demonstrate that costimulation of splenic B cells alters IL-4-induced signal transduction independent of STAT6 leading to proliferation. Furthermore, proliferation induced by IL-4 in LPS-activated blasts is dependent upon the MAP kinase pathway. | ZAMORANO JOSE,Unidad de Investigacion, Hospital San Pedro de Alcantara, Avda Millan Astray, 10003 Caceres ANN E KELLY, JONATHAN AUSTRIAN, HELEN Y WANG, ACHSAH D KEEGAN (Department of Immunology, Jerome Holland Labs, American Red Cross, Rockville, MD, USA | 2001 | Cell Research2001，11，1： | 4 |
| 5 | Zika virus spreading in South America: evolutionary analysis of emerging neutralizing resistant Phe279Ser strains显示文摘Objective: To investigate the genetic diversity of Zika virus(ZIKV) and the relationships existing among these circulating viruses worldwide. To evaluate the genetic polymorphisms harboured from ZIKV that can have an influence on the virus circulation. Methods: Three different ZIKV dataset were built. The first dataset included 63 E gene sequences, the second one 22 NS3 sequences and the third dataset was composed of 108 NS5 gene sequences. Phylogenetic and selective pressure analysis was performed. The edited nucleic acid alignment from the Envelope dataset was used to generate a conceptual translation to the corresponding peptide sequences through UGene software. Results: The phylogeographic reconstruction was able to discriminate unambiguously that the Brazilian strains are belonged to the Asian lineage. The structural analysis reveals instead the presence of the Ser residue in the Brazilian sequences(however already observed in other previously reported ZIKV infections) that could suggest the presence of a neutralization-resistant population of viruses. Conclusions: Phylogenetic, evolutionary and selective pressure analysis contributed to improve the knowledge on the circulation of ZIKV. | Marta Giovanetti Teresa Milano Luiz Carlos Alcantara Laura Carcangiu Eleonora Cella Alessia Lai Alessandra Lo Presti Stefano Pascarella Gianguglielmo Zehender Silvia Angeletti Massimo Ciccozzi | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，5： | 2 |
| 6 | Diosgenin stimulates osteogenic activity by increasing bone matrix protein synthesis and bone-specific transcription factor Runx2 in osteoblastic MC3T3-E1 cells显示文摘 | Ethel H. Alcantara Mee-Young Shin Ho-Yong Sohn Youn-Moon Park Taewan Kim Jae-Hwan Lim Hyung-Jin Jeong Soon-Tae Kwon In-Sook Kwun | 2011 | The Journal of Nutritional Biochemistry2011，，11： | 2 |
| 7 | Space-time surface reconstruction using incompressible flow 显示文摘 | SHARF A ALCANTARA D A LEWINER T | 2008 | ACM Transactions on Graphics2008，27，5： | 1 |
| 8 | Interna- tional inequality in energy intensity levels and the role of produc- tion composition and energy efficiency: An analysis of OECD Countries显示文摘 | Juan Antonio Duro Vicent Alcantara Emilio Padilla | 2010 | Ecological Economics2010，69，12： | 1 |
| 9 | Exercise and the brain: angiogenesis in the adult rat cerebellum after vigorous physical activity and motor skill learning 显示文摘 | Isaacs KR Anderson B J Alcantara AA | 1992 | J Cereb Blood Flow Metab1992，12，1： | 1 |
| 10 | Comparative binding of CrylAb and CrylF Bacillus thuringiensis toxins to brush border membrane proteins from Ostrinia nubilalis, Os- trinia furnacalis and Diatraea saccharalis (Lepidoptera: Crambidae) midgut tissue 显示文摘 | Tan S Y Cayabyab B F Alcantara E P | 2013 | Journal of Invertebrate Pathol- ogy2013，114，3： | 1 |
| 11 | Catalytic production of biodiesel from soybean oil, used frying oil and tallow显示文摘 | Alcantara R Amores J Canoira L | 2000 | Biomass Bioenergy2000，18，： | 1 |
| 12 | Obliquity-correction imaging condition for reverse time migration显示文摘 | Costa J C Silva Neto F A Alcantara M R M | 2009 | Geophysics2009，74，3： | 1 |
| 13 | Role of roots and shoots in the regulation of the Fe efficiency responses in sunflower and cucumber显示文摘 | ROMERA F J ALCANTARA E DELA GUARDIA M D | 1992 | Plant Physiol1992，85，： | 1 |
| 14 | Catalytic production of biodiesel from soy - bean oil:Used frying oil and tallow 显示文摘 | Alcantara R Amores J Canoira L | 2000 | Biomass and Bioenergy2000，18，6： | 1 |
| 15 | Post op erative mediastinitis in a heart hospital of Recife: con tributions for nursing care显示文摘 | Magalhaes MG Alves LM Alcantara LF | 2012 | Revista da Escola de Enfermagem da USP2012，46，： | 1 |
| 16 | Energy and CO《,2》 Emissions in Spain显示文摘 | ROCA J | 1995 | Energy Economics1995，17，3： | 1 |
| 17 | BDNF/MAPK/ERK-induced BMP7 expression in the developing cerebral cortex in duces premature radial glia differentiation and impairs neuronal migration显示文摘 | Ortega J A Alcantara S | 2010 | Cereb Cortex2010，20，9： | 1 |
| 18 | Fabrication and characterization of nanostructured conducting polymer films containing magnetic nanopar- ticles显示文摘 | PATERNO L G FONSECA F J ALCANTARA G B | 2009 | Thin Solid Films2009，517，： | 1 |
| 19 | Genetic Diversity of Fast-growing Rhizobia that Nodulate Soybean ( Glycine max L Merr) 显示文摘 | VIRGINIA G S ALCANTARA M JOSE M | 2003 | Arch Microbiol2003，180，： | 1 |
| 20 | DOTS-Plus for Multidrug-Resistant Tuberculosis in the Philippines: Global Assistance Urgently Needed显示文摘 | Tupasi TE Quelapio MI Orillaza RB Alcantara C Mira NR Abeleda MR Belen VT Arnisto NM Rivera AB Grimaldo ER Derilo JO Dimarucut W Arabit M Urboda D | 2003 | Tuberculosis (Edinb)2003，83，13： | 1 |
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