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	题名	作者	年代	出处	被引量
1	Lack of correlation between Treg quantification assays in inflammatory bowel disease patients显示文摘AIM:To compare the number of regulatory T-cells( Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR(q PCR) method in patients suffering from inflammatory bowel disease(IBD).METHODS:Tregs percentages obtained by both flow cytometry and q PCR methods in 35 adult IBD patients,18 out of them with Crohn′s disease(CD)and 17 with ulcerative colitis(UC)were compared to each other as well as to scores on two IBD activity questionnaires using the Harvey Bradshaw Index(HBI)for CD patients and the Simple Colitis Clinical Activity Index(SCCAI)for UC patients.The Treg percentages by flow cytometry were defined as CD4+CD25highCD127lowFOXP3+cells in peripheral blood mononuclear cells,whereas the Treg percentages by q PCR method were determined as FOXP3 promoter demethylation in genomic DNA.RESULTS:We found an average of 1.56%±0.78%Tregs by using flow cytometry,compared to 1.07%±0.53%Tregs by using q PCR in adult IBD patients.There were no significant correlations between either the percentages of Tregs measured by flow cytometry or q PCR and the HBI or SCCAI questionnaire scores in CD or UC patients,respectively.In addition,there was no correlation between Treg percentages measured by q PCR and those measured by flow cytometry(r=-0.06,P=0.73;Spearman Rho).These data suggest that,either Treg-related immune function or the clinical scores in these IBD patients did not accurately reflect actual disease activity.Until the cause(s)for these differences are more clearly defined,the resultssuggest caution in interpreting studies of Tregs in various inflammatory disorders.CONCLUSION:The two methods did not produce equivalent measures of the percentage of total Tregs in the IBD patients studied which is consistent with the conclusion that Tregs subtypes are not equally detected by these two assays.	Gunnar Brandhorst Darinka Todorova Petrova Sebastian Weigand Christoph Eberle Nicolas von Ahsen Jessica Schmitz Frank Christian Schultze Dirk Raddatz Michael Karaus Michael Oellerich Philip D Walson	2015	World Journal of Gastroenterology2015，21，11：	2
2	Characterization of buried glands before and after radiofrequency ablation by using 3-dimensional optical coherence tomography (with videos)显示文摘	Chao Zhou Tsung-Han Tsai Hsiang-Chieh Lee Tejas Kirtane Marisa Figueiredo Yuankai K. Tao Osman O. Ahsen Desmond C. Adler Joseph M. Schmitt Qin Huang James G. Fujimoto Hiroshi Mashimo	2012	Gastrointestinal Endoscopy2012，，1：	2
3	Assay concordance between SPA and TR-FRET in high-throughput screening显示文摘	von Ahsen O Schmidt A Klotz M	2006	J Biomol Screen2006，11，6：	1
4	Assessment of thyroid disor- ders and autoimmunity in patients with rheumatic diseases 显示文摘	Acay A Ulu MS Ahsen A	2014	Endocr Metab Immune Disord Drug Targets2014，4，3：	1
5	Rapad genotyping of human platelet antigen 5 with fluorophore-labelled hybridization probes on the LightCyeler 显示文摘	Kretzsehmar E Von Ahsen N Trobisch H	2001	Br J Haematol2001，114，2：	1
6	Preservation of Mitochondrial structure and function after Bid-or Bax-mediated cytochrome c release显示文摘	Von Ahsen O Renken C Perkins G	2000	J Cell Biol2000，150，：	1
7	No influence of the MDR -1 C3435T polymorphism or a CYP3A4 promoter polymorphism (CYP3A4 -V allele) on dose -adjusted cyclosporin A trough concentrations or rejection incidence in stable renal transplant recipients 显示文摘	von Ahsen N Richter M Grupp C	2001	Clin Chem2001，47，：	1
8	A novel RNA motif for neomycin recognition显示文摘	Wallis M G von Ahsen U Schroeder R		0，，08：	1
9	Understanding the VOC sorption processes on fluoro alkyl substituted phthalocyanines using ATR FT-IR spectroscopy and QCM measurements显示文摘	Harbeck S EmirikF Gürol I Gürek A G ztürk Z Z Ahsen V	2013	Sensors and Actuators B:Chemical2013，176，83：	1
10	As a new inflammatory marker for familial Mediterranean fever:neutrophil-to-lymphocyte ratio显示文摘	Ahsen A Ulu MS Yuksel S	2013	Inflammation2013，36，6：	1
11	Preclinical evalua-tion of a novel c - Met inhibitor in a gastric cancer xeno-graft model using small animal PET显示文摘	Wiehr S von Ahsen 0 Rose L	2013	Mol Imaging Biol2013，15，2：	1
12	Homocysteine and risk of open-angle glaucoma显示文摘	Bleich S Junemann A yon Ahsen N	2002	J Neural Transm(Vienna)2002，109，12：	1
13	显示文摘	Durmus M Ahsen V Nyokong T	2007	J Photochem Photobiol A2007，186，23：	1
14	No Influence of the MDR-1 C3435T polymorphism or a CYP3A4 promoter polymorphism (CYP3A4 V allele)on dose-adjusted cyclosporin A trough concentrations or rejection incidence in stable renal trans plant recipients显示文摘	Von Ahsen N Richter M Grupp C	2001	Clin Chem2001，47，6：	1
15	As a new inflammatory marker for familial Mediterranean fever:neutrophil to lympho -cyte ratio 显示文摘	Ahsen A Ulu MS Yuksel S	2013	Inflammation2013，36，6：	1
16	Synthesis and crystalline unsymmetrically substituted phthalocyanines显示文摘	YUKSEL F ATILLA D characterization of liquid AHSEN V	2007	Polyhedron2007，26，15：	1
17	No influence of the MDR-1 C3435T polymorphism or a CYP3A4 promoter polymorphism (CYP3A4-V co allele) on doseadjusted eyclosporin Atrough ncentrations or rejection incidence in stable renal transplant recipients 显示文摘	von Ahsen N Richter M Grupp C Ringe B Oellerich M Armstrong VW	2001	Clin Chem2001，47，6：	1
18	Ionoelectrohics cation-induced nonlinear complexation:crown ether-and poly(ethylene oxide)-substituted lutertium bisphthalocyanines显示文摘	Thierry Toupanee Vefa Ahsen Jacques Simon	1994	J Am Chem Soc1994，，116：	1
19	Neutrephil - to - lymphocyte ratio as a quick and reliable predictive marker to diagnose the se- verity of diabetic retinopathy显示文摘	Ulu SM Dogan M Ahsen A	2013	Diabetes Technol Ther2013，15，11：	1
20	No influence of the MDR-l C3435T polymorphism or a CYP3A4 promoter polymorphism (CYP3A4-V allele) on dose-adjusted cyclosporine A trough concentrations or rejection incidence in stable renal transplant recipients显示文摘	VON AHSEN N RICHTER M GRUPP C	2001	Clin Chem2001，47，6：	1

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