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		题名	作者	年代	出处	被引量
	1	Bromelain partitioning in two-phase aqueous systems containing PEO–PPO–PEO block copolymers显示文摘	Ana Paula Brescancini Rabelo Elias Basile Tambourgi Adalberto Pessoa	2004	Journal of Chromatography B2004，，1：	1
	2	Ca^(2+)-亚氨二醋酸金属离子亲和色谱法吸附内毒素(英文)显示文摘Endotoxins(also known as lipopolysaccharides(LPS)) are undesirable by-products of recombinant proteins,purified from Escherichia coli.LPS can be considered stable under a wide range of temperature and pH,making their removal one of the most difficult tasks in downstream processes during protein purification.The inherent toxicity of LPS makes their removal an important step for the application of these proteins in several biological assays and for a safe parenteral administration.Immobilized metal affinity chromatography(IMAC) enables the affinity interactions between the metal ions(immobilized on the support through the chelating compound) and the target molecules,thus enabling high-efficiency separation of the target molecules from other components present in a mixture.Affinity chromatography is applied with Ca2+-iminodiacetic acid(IDA) to remove most of the LPS contaminants from the end product(more than90%).In this study,the adsorption of LPS on an IDA-Ca2+ was investigated.The adsorption Freundlich isotherm of LPS-IDA-Ca2+ provides a theoretical basis for LPS removal.It was found that LPS is bound mainly by interactions between the phosphate group in LPS and Ca2+ ligands on the beads.The factors such as pH(4.0 or 5.5) and ionic strength(1.0 mol/L) are essential to obtain effective removal of LPS for contaminant levels between endotoxin' concentration values less than100 EU/mL and 100 000 EU/mL.This new protocol represents a substantial advantage in time,effort,and production costs.	André Moreni LOPES Jorge Sánchez ROMEU Rolando Páez MEIRELES Gabriel Marquez PERERA Rolando Perdomo MORALES Adalberto PESSOA Lourdes Zumalacárregui CáRDENAS	2012	色谱2012，30，11：	1

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