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| 1 | 二维波穿过单节理的透射率特性及其隐含意义显示文摘二维波穿过单节理的问题是一个复杂的多因素影响问题,其透射率影响因素有:节理无量纲刚度、射线角、巷道半径、所在点离巷道中心的径向距离以及岩石力学性能参数.对二维波穿过单节理的透射率进行了详细的算例分析.归纳了二维波穿过单节理后透射率特性,在此基础上提出了相关问题的研究策略.分析结果表明:单节理后各节点透射率的研究,可以通过单节理后某一以巷道或钻孔中心为中心的圆弧上各节点的透射率来展开;射线角为90°的射线上节理后各节点透射率的研究,可以通过节理后某一节点的透射率来展开;单节理的透射率的研究可以在一个固定半径值的巷道或钻孔的模型上用一种典型的岩石进行. | 雷卫东 ASHRAF M H 滕军 赵坚 宋宏伟 | 2006 | 中国矿业大学学报2006，35，4： | 7 |
| 2 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 3 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 4 | Osmotic adjustment in sorghum under NaCl stress 显示文摘 | KHAN A H ASHRAF M Y AZMI A R | 1992 | Physiol Plant1992，14，： | 1 |
| 5 | Finite Element Model Updating Approach to Damage Identification in Beams Using Par- ticle Swarm Optimization 显示文摘 | Mohamed M S Mustafa H A Ashraf O | 2013 | Engineering Optimization2013，45，6： | 1 |
| 6 | Recent developments in biodegradation of industrial pollutants by white rot fungi and their enzymesystem显示文摘 | ASGHER M BHATTI H N ASHRAF M | 2008 | Biodegradation2008，19，6： | 1 |
| 7 | Microstructure and electrical properties of 5m203 doped Bi203-based ZnO varistor ceramics 显示文摘 | ASHRAF M A BHUIYAN A H HAKIM M A HOSSAIN M T | 2011 | Materials Science and Engineering B2011，176，11： | 1 |
| 8 | Recent developments in biodegradation of industrial pollutants by white rot fungi and their enzyme system显示文摘 | ASGHER M BHATTI H N ASHRAF M | 2008 | Biodegradation2008，19，6： | 1 |
| 9 | Ca2 + as a mediator of ischemic preconditioning显示文摘 | Miyawaki H Ashraf M | 1997 | Circ Res1997，80，6： | 1 |
| 10 | The cyclecharacteristics of clomiphene with clomiphene andmenotropins in polycystic ovary syndrome and nonpolycystic ovary syndrome infertile patients 显示文摘 | Ghasemi M Ashraf H Koushyar H | 2013 | MinervaGinecol2013，65，3： | 1 |
| 11 | Microstructure and electrical properties of HO203 doped Bi203-based ZnO varistor ceramics 显示文摘 | ASHRAF M A BHUIYAN A H HAKIM M A HOSSA1N M T | 2010 | Physica B: Condensed Matter2010，405，17： | 1 |
| 12 | Recent advances in silicon etching for MEMS using the ASETM process 显示文摘 | HYNES A M ASHRAF H BHARDWAJ J K | 1999 | Sensors and Actuators A : Physical1999，74，13： | 1 |
| 13 | Diazoxide potentiates mesenchymal stem cell survival via NF-kappa B-dependent miR-146a expression by targeting Fas显示文摘 | Suzuki Y Kim H W Ashraf M | 2010 | Am J Physiol-Heart Cir Physiol2010，299，： | 1 |
| 14 | Some prospective strategies for improving crop salt tolerance显示文摘 | Ashraf M Athar H R Harris P J C | 2008 | Advances in Agronomy2008，97，： | 1 |
| 15 | Impact of desalination plants brine injection wells on coastal aquifers显示文摘 | MOHAMED K K N REDA M E ASHRAF H M G | 2008 | Environment Geology2008，54，3： | 1 |
| 16 | Efficient non - viral reprogramming of myoblasts to stemness with a single small molecule to generate cardiac progenitor cells显示文摘 | Pasha Z Haider H Ashraf M | 2011 | PloS one2011，6，23： | 1 |
| 17 | Calcium Preconditioning elicits strong protection against ischemic injury via protein kinase C signaling pathway显示文摘 | Zhou X Ashraf M | 1996 | Cir Res1996，74，： | 1 |
| 18 | Some prospective strategies for improving crop salt tolerance显示文摘 | Ashraf M Athar H R Harris P J C | 2008 | Advances in Agronomy2008，97，： | 1 |
| 19 | Isoproterenol mimics calcium preconditioning - induced protection against ischemia显示文摘 | Ashraf M | 1997 | Am J Physiol1997，272，22： | 1 |
| 20 | Ca2 + as a mediator of Ischemic Preconditioning显示文摘 | Zhou X Ashraf M | 1997 | Cir Res1997，80，： | 1 |
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