|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Comparative evaluation of microscopy,OptiMAL® and 18S rRNA gene based multiplex PCR for detection of Plasmodium falciparum & Plasmodium vivax from field isolates of Bikaner,India显示文摘Objective:To evaluate microscopy,OptiMAL? and multiplex PCR for the identification of Plasmodium falciparum(P.falciparum) and Plasmodium vivax(P.vivax) from the field isolates of Bikaner,Rajasthan(Northwest India).Methods:In this study,a multiplex PCR(P.falciparum and P.vivax) was further developed with the incorporation of Plasmodium malariae(P.malariae) specific primer and also a positive control.The performance of microscopy,plasmodium lactate dehydrogenase(pLDH) based malaria rapid diagnostic test OptiMAL? and 18S rRNA gene based multiplex PCR for the diagnosis of P.falciparum and P.vivax was compared.Results:The three species multiplex PCR if.falciparum,P.vivax and P.malariae) with an inbuilt positive control was developed and evaluated.In comparison with multiplex PCR,which showed the sensitivity and specificity of 99.36%(95%CI,98.11%-100.00%) and 100.00%(95%CI,100.00%-100.00%),the sensitivity and specificity of microscopy was 90.44%(95%CI,88.849-95.04%) and 99.22%(95% CI,97.71%-100.00%),and OptiMAL? was 93.58%(95%CI,89.75%-97.42%) and 97.69%(95%CI, 95.10%-100.00%).The efficiencies were 99.65%,95.10%and 95.45%for multiplex PCK.microscopy and OptiMAL?.respectively.Conclusions:Our results raise concerns over the overall sensitivities of microscopy and OptiMAL?,when compared to the multiplex PCR and thus stress the need for new molecular interventions in the accurate detection of the malarial parasites.This further highlights the fact that further developments are needed to improve the performance of rapid diagnostic tests at field level. | Deepak Pakalapati Shilpi Garg Sheetal Middha Abhishek Kochar Amit Kumar Subudhi Boopathi Pon Arunachalam Sanjay Kumar Kochar Vishal Saxena Pareek RP Jyoti Acharya Dhanpat Kumar Kochar Ashis Das | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，5： | 3 |
| 2 | A novel ZnO/reduced graphene oxide and Prussian blue modified carbon paste electrode for the sensitive determination of Rutin显示文摘A carbon paste modified sensor based on a novel composite of zinc oxide nanoparticles deposited on reduced graphene oxide(ZnO-rGrO) and Prussian blue(PB) was drop-cast(ZnO-rGrO-PB/MCPE) for the sensitive estimation of Rutin(Rtn) at pH 7.0.The high surface area of ZnO-rGrO and electrocatalytic property of PB promotes the oxidation of Rtn. Field emission scanning electron microscope(FE-SEM) and energy-dispersive X-ray spectroscopy(EDX) techniques were employed to confirm the deposition of ZnO-rGrO and PB on carbon paste electrode(CPE). The ability of ZnO-rGrO-PB/MCPE in charge transfer at the interface was investigated using electrochemical impedance spectroscopy(EIS). The heterogeneous rate constant(ks) and the charge transfer coefficient(α) have been calculated as 6.08 s^(-1) and 0.74 respectively. This sensor showed a wide linear response for Rtn from 7.0×10^(-8)to 7.0×10^(-6) M and 7.0×10^(-6) to 1.0×10^(-4) M with a limit of detection(2.05±0.04)×10^(-8) M(S/N=3). The application of ZnO-rGrO-PB/MCPE was found in the analysis of Rtn in fruit juice samples using standard addition method. This sensor showed good reproducibility, stability, selectivity and sensitivity. | Ozma J D'Souza Ronald J Mascarenhas Ashis K Satpati Basavanakote M Basavaraja | 2019 | Science China Chemistry2019，62，2： | 2 |
| 3 | Assessment of mosquito larvicidal potency of cyclic lipopeptides produced by Bacillus subtilis strains显示文摘 | Kishore Das Ashis K. Mukherjee | 2005 | Acta Tropica2005，，2： | 2 |
| 4 | Fabrication of the electrochemically reduced graphene oxide-bismuth nanoparticles composite and its analytical application for an anticancer drug gemcitabine显示文摘The present study explores an electroreduced graphene oxide-bismuth nanoparticles composite(ErGOBi) as an electrochemical sensor for the determination of an anticancer drug, gemcitabine hydrochloride(GMB). The Er-GOBi interface was prepared by drop casting of bismuth nitrate-graphene oxide suspension on a glassy carbon electrode(GCE) followed by electro-reduction in the potential range of 0.6 V to 1.7 V. SEM, FTIR, EDAX and AFM techniques were employed for the characterization of prepared materials. Cyclic voltammetric and electrochemical impedance spectroscopic methods were used to understand the charge transfer properties of stepwise modification of Er-GOBi/GCE. GMB exhibited an irreversible oxidation peak at 1.144 V on Er-GOBi/GCE in phosphate buffer of p H 3. A 100-fold enhanced oxidation peak current was observed at Er-GOBi/GCE when compared to that at bare GCE.Sensing performance of Er GO-Bi/GCE was optimized by varying peak current dependent parameters.Linear relationship between the peak current and concentration of GMB was observed in the range of 0.1–51.1 mmol/L in differential pulse voltammetric method and 2.1–61.1 mmol/L in linear sweep voltammetric method. The practical utility of the proposed sensor, Er-GOBi/GCE was demonstrated by determining GMB in pharmaceutical formulations and spiked urine samples. | Ranjita Tandel Nagappa Teradal Ashis Satpati Seetharamappa Jaldappagari | 2017 | Chinese Chemical Letters2017，28，7： | 2 |
| 5 | Intranasal ketamine for the treatment of patients with acute pain in the emergency department显示文摘BACKGROUND:Pain in the emergency department(ED)is common but undertreated.The objective of this study was to examine the efficacy and safety of intranasal(IN)ketamine used as an analgesic for patients with acute injury with moderate to severe pain.METHODS:This study was a cross sectional,observational study of patients more than 8 years old experiencing moderate to severe pain[visual analog score(VAS)>50 mm].The initial dose of IN ketamine was 0.7 mg/kg with an additional dose of 0.3 mg/kg if VAS was more than 50 mm after 15minutes.Pain scores and vital signs were recorded at 0,15,30 and 60 minutes.Side-effects,sedation level and patient's satisfaction were also recorded.The primary outcome was the number of patients achieving≥20 mm reductions in VAS at 15 minutes.Other secondary outcome measures were median reduction in VAS at 15,30 and 60 minutes,changes of vital signs,adverse events,satisfaction of patients,and need for additional ketamine.RESULTS:Thirty-four patients with a median age of 29.5 years(IQR 17.5–38)were enrolled,and they had an initial median VAS of 80 mm(IQR 67–90).The VAS decreased more than 20 mm at15 minutes in 27(80%)patients.The reduction of VAS from baseline to 40 mm(IQR 20–40),20 mm(IQR 14–20)and 20 mm(IQR 10–20)respectively at 15,30 and 60 minutes(P<0.001).No critical changes of vital signs were noted and adverse effects were mild and transient.CONCLUSION:This study showed that IN ketamine is an analgesic choice for patients with acute injury in moderate to severe pain in an overcrowded and resource limited ED. | Roshana Shrestha Samita Pant Ashis Shrestha Kabita Hada Batajoo Rashmi Thapa Sumana Vaidya | 2016 | World Journal of Emergency Medicine2016，7，1： | 2 |
| 6 | Ecological significance and some biotechnological applieation of an organic solvent stable alkaline serine protease from Bacillus subtilis strain DM-04 显示文摘 | Sudhir KR Ashis KM | 2009 | Bioresour Technol2009，100，9： | 1 |
| 7 | Crude petroleum-oil biodegradation effciency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India显示文摘 | Kishore Das Ashis K Mukherjee | 2007 | Bioresource Technology2007，98，： | 1 |
| 8 | Application of high hydrostatic pressure to control enzyme related fresh seafood texture deterioration 显示文摘 | ASHIE I N A SIMPSON B K | 1996 | Food Res Int1996，29，516： | 1 |
| 9 | Improvement of tensile properties of wrought magnesium alloys by grain refining显示文摘 | Kamado S Ashie T Yamada H | 2000 | Mater Sci Forum2000，35，： | 1 |
| 10 | A simple way to identify insulin resistance in non-diabetic acute coronary syndrome patients with impaired fasting glucose显示文摘 | Sayantan Ray Ashit Bairagi Santanu Guha Satyabrata Ganguly Debes Ray Ashis Basu Anirban Sinha | 2012 | Indian Journal of Endocrinology and Metabolism2012，，8： | 1 |
| 11 | Effects of papain and a microbial enzyme on meat proteins and beef tenderness显示文摘 | ASHIE I N A SORENSEN T L NIELSEN P M | 2002 | Food Science2002，67，： | 1 |
| 12 | Utilization of chitosanfor preservation for raw shrimp (Pandalus borealis)显示文摘 | SIMPSON B K GAGNE N ASHIE INA | 1997 | FoodBiotechnology1997，11，1： | 1 |
| 13 | Microbial biomass and itsactivities in salt-affected coastal soils 显示文摘 | SUDIPTA T SABITRI K ASHIS C | 2006 | Biology and Fertility ofSoils2006，42，： | 1 |
| 14 | Improvement of tensile properties of wrought magnesium alloys by grain refining 显示文摘 | Kamado S Ashie T Yamada H | 2000 | Materials Science Forum2000，35，： | 1 |
| 15 | Evaluation of quality of life and psychological response in can cer patients treated withradiotherapy 显示文摘 | Takah ashi T Hondo M Nishinura K | 2008 | RadiatM ed2008，26，7： | 1 |
| 16 | Suppression of lipid hydroperoxide induced oxidative damage to cellular DNA by es culetin显示文摘 | Kaneko T Tahara S Takaba:y'ashi F | 2003 | Biol Pharm Bull2003，26，6： | 1 |
| 17 | Crude petroleum-oil biodeg-radation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India显示文摘 | KISHOREE D ASHIS K M | 2007 | Bioresource Technoi2007，98，7： | 1 |
| 18 | Kinetics of substitution of aquo ligands fromcis-diaquo-bis-(biguanide) cobalt(III) and chromium(III) ions by aspartic acid in ethanol—water mixtures显示文摘 | Ashis Kumar Gangopadhyay Gauri Sankar De | 1989 | Transition Metal Chemistry1989，，： | 1 |
| 19 | 显示文摘 | Kamado S Ashie T Ohshima Y | 2000 | Master Sci Forum2000，，： | 1 |
| 20 | Stock markets,corporate finance,and economic growth:An Overview显示文摘 | Ashi Demigüc-Kunt Ross L | 1996 | The World Bank Economic Review1996，10，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员