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| 1 | Translational pancreatic cancer research:a comparative study on patient-derived xenograft models显示文摘AIM To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice.METHODS This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were obtained surgically from patients with a pathological diagnosis of pancreatic adenocarcinoma. Tumour samples from pancreatic cancer patients were transplanted into nude mice in three different locations(intraperitoneal, subcutaneous and pancreatic). Histological analysis(haematoxylin-eosin and Masson's trichrome staining) and immunohistochemical assessment of apoptosis(TUNEL), proliferation(Ki-67), angiogenesis(CD31) and fibrogenesis(α-SMA) were performed. When a tumour xenograft reached the target size, it was reimplanted in a new nude mouse. Three sequential tumour xenograft generations were generated(F1, F2 and F3).RESULTS The overall tumour engraftment rate was 61.1%. The subcutaneous model was most effective in terms of tissue growth(69.9%), followed by intraperitoneal(57.6%) and pancreatic(55%) models. Tumour development was faster in the subcutaneous model(17.7 ± 2.6 wk) compared with the pancreatic(23.1 ± 2.3 wk) and intraperitoneal(25.0 ± 2.7 wk) models(P = 0.064). There was a progressive increase in the tumour engraftment rate over successive generations for all three models(F1 28.1% vs F2 71.4% vs F3 80.9%, P < 0.001). There were no significant differences in tumour xenograft differentiation and cell proliferation between human samples and the three experimental models among the sequential generations of tumour xenografts. However, a progressive decrease in fibrosis, fibrogenesis, tumour vascularisation and apoptosis was observed in the three experimental models compared with the human samples. All three pancreatic patient-derived xenograft models presented similar histological and immunohistochemical characteristics.CONCLUSION In our experience, the faster development andgreatest number of viable xenografts could make the subcutaneous model the best option for experimentation in pancreatic cancer. | Mercedes Rubio-Manzanares Dorado Luis Miguel Marín Gómez Daniel Aparicio Sánchez Sheila Pereira Arenas Juan Manuel Praena-Fernández Juan Jose Borrero Martín Francisco Farfán López Miguel ángel Gómez Bravo Jordi Muntané Relat Javier Padillo Ruiz | 2018 | World Journal of Gastroenterology2018，24，7： | 2 |
| 2 | Initial experience with EUS-guided cholangiopancreatography for biliary and pancreatic duct drainage: a Spanish national survey显示文摘 | Juan J. Vila Manuel Pérez-Miranda Enrique Vazquez-Sequeiros Monder Abu-Suboh Abadia Antonio Pérez-Millán Ferrán González-Huix Joan Gornals Julio Iglesias-Garcia Carlos De la Serna José R. Aparicio José C. Subtil Alberto álvarez Felipe de la Morena Jesús G | 2012 | Gastrointestinal Endoscopy2012，，6： | 2 |
| 3 | Pharmacogenomics in lung cancer: an analysis of DNA repair gene expression in patients treated with platinum-based chemotherapy 显示文摘 | Garcia-Campelo R Alonso-Curbera G Anton Aparicio L M | 2005 | Expert Opin Pharmacother2005，6，12： | 1 |
| 4 | Epigenetics in human disease and prospects for epigenetic therapy显示文摘 | Egger G Liang GN Aparicio A | 2004 | Nature2004，429，6990： | 1 |
| 5 | Epigenetics in human disease and prospects for epigenetic therapy显示文摘 | Egger G Liang G Aparicio A Jones PA | 2004 | Nature2004，429，6990： | 1 |
| 6 | Epigenetics in human disease and prospects for epigenetic therapy 显示文摘 | Egger G Liang G Aparicio A | 2004 | Nature2004，429，6990： | 1 |
| 7 | Searching for Hif1-αinteracting proteins in renal cellcarcinoma显示文摘 | Medina Vil aamil V Aparicio Gal ego G Santamarina Caínzos I | | 0，，09： | 1 |
| 8 | Epigenetics in human disease and prospects for epigenetic therapy显示文摘 | EGGER G LIANG G APARICIO A | 2004 | Nature2004，429，： | 1 |
| 9 | Testing of segmental concrete girders with external tendons显示文摘 | RABBAT B G APARICIO A C | 1987 | PCI Jour- nal1987，32，1： | 1 |
| 10 | Directed evolution of beta - glucosidase A from Paenibacillus poly- myxa to thermal resistance显示文摘 | Gonzalez - Blasco G Sanz - Aparicio J Gonzalez B | 2000 | The Journal of Biological Chemistry2000，275，13： | 1 |
| 11 | Expression of Notchl to - 4 and their ligands in renal cell carcinoma_ a tissue microarray study 显示文摘 | Aparicio L Villaamil V Gallego G | 2011 | Cancer Genomics Proteomics2011，8，2： | 1 |
| 12 | Ultimate behavior of externally prestressed concrete bridges 显示文摘 | Ramos G Aparicio A C | 1995 | Structural Engineering International1995，，3： | 1 |
| 13 | Flexural strength of externally prestressed concrete bridges显示文摘 | Aparicio A C Ramos G | 1996 | ACI Structural Journal1996，93，5： | 1 |
| 14 | Epigenetics in human disease and prospects for epigenetic therapy 显示文摘 | Egger G Liang C Aparicio A | 2004 | Nature2004，429，6990： | 1 |
| 15 | Epigenetics in human disease and prospects for epigenetic therapy 显示文摘 | Egger G Liang Gangning Aparicio A | 2004 | Nature2004，429，6990： | 1 |
| 16 | Epigenetics in human disease and prospects for epigenetic therapy显示文摘 | Egger G Liang G Aparicio A | 2004 | Nature2004，429，6990： | 1 |
| 17 | Epigenetics in human disease and prospects for epigenetic therapy显示文摘 | Egger G Liang G Aparicio A Jones P A | 2004 | Nature2004，429，： | 1 |
| 18 | Epigenetics in human disease and prospects for epigenetic therapy显示文摘 | Egger G Liang G Aparicio A | 2004 | Nature2004，429，： | 1 |
| 19 | Tracing out the northern tidal stream of the Sagittarius dwarf spheroidal galaxy显示文摘 | Martínez-Delgado D Gómez-Flechoso M(A) Aparicio A | 2004 | The Astrophysical Journal2004，601，： | 1 |
| 20 | Potential benefits of renal diets on cardiovascular risk factors in chronic kidney disease patients显示文摘 | CUPISTI A APARICIO M BARSOTTI G | | 0，，05： | 1 |
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