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| 1 | HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays显示文摘AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma. | Lucas Faria Abraho-Machado Alexandre Andrade dos Anjos Jácome Durval Renato Wohnrath José Sebastio dos Santos Estela Cristina Carneseca José Humberto Tavares Guerreiro Fregnani Cristovam Scapulatempo-Neto | 2013 | World Journal of Gastroenterology2013，19，38： | 12 |
| 2 | Bartter's syndrome:clinical findings,genetic causes and therapeutic approach显示文摘Backgound Bartter's syndrome(BS)is a rare group of salt losing tubulopathies due to the impairment of transport mecha-nisms at the thick ascending limb of the Henle's loop.Data sources Literature reviews and original research articles were collected from database,including PubMed and Scopus.Results According to the time of onset and symptoms,BS can be classified into antenatal and classic BS.Molecular studies have identified different subtypes of BS.BS types Ⅰ,Ⅱ and Ⅲ are caused by mutations on genes encoding the luminal Na^(+)-K^(+)-2Cl^(-) co-transporter,the luminal K+ channel ROMK,and the basolateral chloride channel ClC-Kb(CLCNKB),respectively.Loss-of-function mutations of Barttin CLCNK type accessory beta subunit cause BS type Ⅳa.Simultaneous mutations of CLCNKB and CLCNKA cause BS type Ⅳb.BS type Ⅴ consists in a novel transient form characterized by antenatal presentation due to mutations in the MAGE family member D2.Severe gain-of-function mutations of the extracellular calcium sensing receptor gene can result in an autosomal dominant condition of BS.Main clinical and biochemical alterations in BS include polyuria,dehydration,hypokalemia,hypochloremic metabolic alka-losis,hyperreninemia,high levels of prostaglandins,normal or low blood pressure,hypercalciuria and failure to thrive.Treatment focuses mainly at correcting dehydration and electrolyte disturbances and in measures to reduce polyuria,including the use of nonsteroidal anti-inflammatory medications to control excessive renal prostaglandin E2 production.Conclusions Early diagnosis and treatment of BS may prevent long-term consequences such as growth failure,nephrocal-cinosis and end-stage renal disease. | Flavia Cristina Carvalho Mrad Sílvia Bouissou Morais Soares Luiz Alberto Wanderley de Menezes Silva Pedro Versiani dos Anjos Menezes Ana Cristina Simoes-e-Silva | 2021 | World Journal of Pediatrics2021，17，1： | 10 |
| 3 | 酶在皮革工业中的应用综述显示文摘当今皮革行业面临着新的挑战,包括改善和优化生产过程来满足皮革制品对皮革质量的要求,以及遵循环保法规的相关规定。酶处理皮革是一项非常有前途的技术。酶是有机物质,通常是蛋白质,作为一种生物催化剂能催化复杂的化学反应。酶广泛应用于洗涤剂、食品、医药、精细化工等行业。通常,理想的酶源是微生物。一些研究报导酶用于皮革生产的不同阶段,能够减少在脱毛和浸灰过程中化学品的使用,甚至有报导称酶可以完全替代化学品。一些学者将酶用于染浴过程中来提高染料吸收率和用于鞣制过程来提高鞣制效率。一种新酶的鉴定是一个漫长的过程,需要筛选和分离出符合特定条件的新细菌,并且掌握所选微生物生长所需的营养和其他必要条件。酶应用于制革生产过程中,相关参数如pH、温度和产物对酶的抑制作用等影响,应该做进一步的研究。 | ALINE DETTMER PATRICIA SCHACKER DOS ANJOS MARILIZ GUTTERRES 杜卫宁 | 2013 | 西部皮革2013，，22： | 4 |
| 4 | Diagnosis and Objective Pain Assessment of Traditional Chinese Medicine May Be Useful to Demonstrate Specific Effects of Acupuncture in Low Back Pain: A Prospective, Randomized, Controlled and Single Blinded Pre-study显示文摘Objective: To prove specific effects of acupuncture on chronic pain. It was speculated that the potential specific effectiveness of acupuncture could be better shown in more properly designed studies. Therefore, diagnoses of both Western medicine and traditional Chinese medicine (TCM) were used as inclusion criteria to allocate acupoints more precisely to the complaints of the patients. Secondly, objectively measurable parameters of pain relief in addition to usual Visual Analogue Scale (VAS) were chosen to quantify the effects of acupuncture. Methods: The study was prospective, randomized, controlled and single-blinded. Eighteen patients with chronic back pain and TCM diagnosis of a Taiyang/Yangming syndrome received one single session of acupuncture chosen according to TCM diagnosis or acupuncture on points outside the meridian system (controls). We evaluated pain via VAS and increased mobility via inclinometry of the back. Results: Although the sample size was small, there was a statistically significant improvement in pain and mobility in the intervention group (n=13), but not in the control group (n=5). The calculated sample size adequate power was lower for inclinometry than for VAS, indicating that VAS is less probable to discriminate acupuncture effects. Conclusion: Objectively measurable physical parameters such as the angle of flexion before and after acupuncture (inclinometry) may be more suitable to measure pain relief than subjective assessment by VAS in acupuncture studies. TCM diagnosis may be a helpful inclusion criterion in studies on acupuncture, so as to potentially allocate interventions better to the complaints of patients. | Susana Seca Paula Capelo Thomas Efferth Christoph Alexander Doenitz Sven Schroeder Ana Anjos Jorge Machado Henry Johannes Greten | 2013 | Journal of Acupuncture and Tuina Science2013，11，3： | 2 |
| 5 | Development and Optimization of an Alternative Methodology for Detection of Milk Adulteration by Water显示文摘 | Wesley William Gongalves Nascimento Marcone Augusto Leal de Oliveira1 Marcone Augusto Leal de Oliveira Marco Antonio Moreira Furtado Virgilio de Carvalho dos Anjos Maria Jose Valenzuela Bell | 2013 | Journal of Food Science and Engineering2013，3，7： | 2 |
| 6 | Prognostic value of epidermal growth factor receptors in gastric cancer: a survival analysis by Weibull model incorporating long-term survivors显示文摘 | Alexandre Andrade Anjos Jácome Durval R. Wohnrath Cristovam Scapulatempo Neto Estela C. Carneseca Sérgio V. Serrano Luciano Souza Viana Jo?o S. Nunes Edson Z. Martinez José Sebasti?o Santos | 2014 | Gastric Cancer2014，，1： | 2 |
| 7 | 量化中医学中“寒”与“热”的初步临床研究(英文)显示文摘目的:本研究探讨通过测量微循环相关指标客观衡量针刺效果的可能性,中医寒证针刺治疗前毛细血管灌注状态的意义,以及微循环相关指标在未来中医诊断中的意义。方法:本研究为前瞻性、非对照、不设盲临床研究。研究共纳入32例股骨骨折手术后的老年患者。这些患者均接受施于胃经梁丘穴的'豹斑点刺'针刺治疗。针刺治疗前后使用白光光谱学及激光多普勒测量微循环相关指标如血流速率、血流量、血红蛋白含量、氧饱和度等。结果:股骨骨折后,根据血流量的不同,患者被分为两组,即高灌注组和低灌注组。低灌注组的毛细血管血流量及血流速率在针刺治疗后明显增加,而高灌注组则无明显改变。如果不考虑患者在针刺治疗前的灌注状态,则统计分析表明所有患者针刺治疗前后的灌注状态均无明显改变,因此针刺的作用可能被患者的异质性所掩盖。结论:微循环相关指标对于客观衡量针刺的治疗效果及描述针刺治疗前的营养性功能以均衡比较组之间的差异有重要意义。在本试验中,局部寒证(腿部的毛细血管低灌注)可以通过针刺补气血的胃经梁丘穴得到显著改善,而在热证组(毛细血管高灌注)未见明显改变。未来的研究可以通过将中医营养参数测量的营养状态与中医诊断中的主要证候相联系而得到更多结论。 | Christoph A.Doenitz Ana Anjos Thomas Efferth Tobias Greten Henry J.Greten | 2012 | 中西医结合学报2012，10，5： | 2 |
| 8 | Low- level laser therapy in IL-1显示文摘 | de Jesus JF Spadacci-Morena DD Dos Anjos Rabelo ND | 2015 | Lasers Med Sci2015，30，1： | 1 |
| 9 | Detection of harmful cyanobacteria and their toxins by both PCR amplification and LC-MS during a bloom event显示文摘 | Bittencourt-Oliveira MD Zajac MP | 2006 | Toxicon2006，48，3： | 1 |
| 10 | Independent expression of the alpha and beta c-erbA genes in developing rat brain 显示文摘 | Mellstrom JR Nas'anjo A Santos | 1991 | Mol Endocrinol1991，5，9： | 1 |
| 11 | The benefits of cardiac rehabilitation in coronary heart disease: A gender issue? 显示文摘 | Diana Anjo M a rio Santos Parr f cia Rodrigues | 2014 | Revista Portuguesa de Cardiologia2014，33，2： | 1 |
| 12 | Mechanical ventilation with high tidal volume induces inflam- marion in pations without lung disease 显示文摘 | Pinheirode Oliveira R Hetzel MP dos Anjos Silva M | 2010 | Cfit Care Med2010，14，： | 1 |
| 13 | Neoadjuvant chemotherapy with six cycles of carbo- platin and paclitaxel in advanced ovarian cancer patients unsuitable for primary surgery:safety and effectiveness显示文摘 | da Costa Miranda V de Souza Forde hB Dos Anjos CH | 2014 | Gynecol Oncol2014，132，2： | 1 |
| 14 | Omega 3 fatty acids on child growth,visual acuity and neurodevelop- ment显示文摘 | Campoy C Escolano-Margarit MV Anjos T | 2012 | Br J Nutr2012，107，2： | 1 |
| 15 | The use of PDCAmethod and quality tools in agribusiness management in MatoGrosso do Sul State显示文摘 | Anjos MC Souza CC Cezar IM | 2012 | Revista Agrarin2012，5，15： | 1 |
| 16 | Me- chanical ventilation with high tidal volume induces inflammation in patients without lung disease显示文摘 | Pinheiro de Oliveira R Hetzel MP dos Anjos Silva M | 2010 | Crit Care2010，14，2： | 1 |
| 17 | Chlorite au- thigenesis and porosity preservation in the Upper Creta- ceous marine sandstones of the Santos Basin, offshore eastern Brazil显示文摘 | Anjos S M C De Ros L F Silva C M A | 2003 | International Association of Sedimento- logy Special Publication2003，34，2： | 1 |
| 18 | Temperature dependence of electroluminescence degradation in organic light emitting devices without and with a copper phthalocyanine buffer layer 显示文摘 | Anjos P N Aziz H Hu N | 2002 | Organic Electronics2002，3，： | 1 |
| 19 | Fetal liver stroma consists of cells in epithelial-to-mesenchymal transition显示文摘 | Chagraoui J Lepage NA Anjo A | 2003 | Blood2003，101，8： | 1 |
| 20 | Depositional and diagenetic controls on the reservoir quality of Lower Creta- ceous Pendeneia sandstones, Potiguar rift basin, Brazil显示文摘 | dos Anjos S M C de Ros L F de Souza R S | 2000 | AAPG Bulletin2000，84，11： | 1 |
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