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| 1 | Antioxidant Effect of Sepia Ink Extract on Extrahepatic Cholestasis Induced by Bile Duct Ligation in Rats显示文摘Objective The aim of our study was to assess the complications of hepatic fibrosis associated with bile duct ligation and the potential curative role of sepia ink extract in hepatic damage induced by bile duct ligation. Methods Rattus norvegicus rats were divided into 3 groups: Sham-operated group, model rats that underwent common bile duct ligation(BDL), and BDL rats treated orally with sepia ink extract(200 mg/kg body weight) for 7, 14, and 28 d after BDL. Results There was a significant reduction in hepatic enzymes, ALP, GGT, bilirubin levels, and oxidative stress in the BDL group after treatment with sepia ink extract. Collagen deposition reduced after sepia ink extract treatment as compared to BDL groups, suggesting that the liver was repaired. Histopathological examination of liver treated with sepia ink extract showed moderate degeneration in the hepatic architecture and mild degeneration in hepatocytes as compared to BDL groups. Conclusion Sepia ink extract provides a curative effect and an antioxidant capacity on BDL rats and could ameliorate the complications of liver cholestasis. | Hanan Saleh Amel M Soliman Ayman S Mohamed Mohamed-Assem S Marie | 2015 | Biomedical and Environmental Sciences2015，28，8： | 6 |
| 2 | Potential predictive factors for pathologic complete response after the neoadjuvant treatment of rectal adenocarcinoma:a single center experience显示文摘Objective:To assess the response rate of patients with rectal adenocarcinoma to neoadjuvant therapy and to identify the predictors of histological regression after neoadjuvant radiotherapy(RT)or concurrent chemoradiotherapy(CCRT).Methods:This study recruited 64 patients.The patients had resectable cancer of the lower and the middle rectum(T3/T4 and/or N+)without distant metastasis and received neoadjuvant RT or CCRT followed by radical surgery with total mesorectal excision(TME)between January 2006 and December 2011.The patients were classified into non-response(NR),partial response(PR),and pathologic complete response(p CR)based on the Dworak tumor regression grading system.Results:The median age of patients was 57 years(ranging from 22 to 85).A total of 24 patients were treated with neoadjuvant CCRT,whereas 40 patients were treated with RT alone.Abdominoperineal resection(APR)was performed on 29 patients(45%).Anterior resection with TME was performed on 34 patients(53%).One patient had local resection.Histologically,12(19%),24(73%),and 28(44%)patients exhibited p CR,PR,and NR,respectively.Univariate analysis revealed that the predictors of tumor regression were as follows:the absence of lymph node involvement from initial imaging(c N0)(P=0.021);normal initial carcinoembryonic antigen(CEA)level(P=0.01);hemoglobin level≥12 g/dl(P=0.009);CCRT(P=0.021);and tumor downstaging in imaging(P=0.001).Multivariate analysis showed that the main predictors of p CR were CT combined with neoadjuvant RT,c N0stage,and tumor regression on imaging.Conclusions:Identifying the predictors of p CR following neoadjuvant therapy aids the selection of responsive patients for nonaggressive surgical treatment and possible surveillance. | Feryel Letaief Meher Nasri Mouna Ayadi Khedija Meddeb Amina Mokrani Yosra Yahyaoui Nesrine Chraiet Henda Raies Amel Mezlini | 2017 | Cancer Biology & Medicine2017，14，3： | 3 |
| 3 | Impact of Metformin on the Prognosis of Cirrhosis Induced by Viral Hepatitis C in Diabetic Patients显示文摘 | Gisèle Nkontchou Emmanuel Cosson Mounir Aout Amel Mahmoudi Valérie Bourcier Iliass Charif Nathalie Ganne-Carrie Véronique Grando-Lemaire Eric Vicaut Jean-Claude Trinchet Michel Beaugrand | 2011 | The Journal of Clinical Endocrinology & Metabolism2011，，8： | 3 |
| 4 | Diagnostic accuracy of FibroScan and comparison to liver fibrosis biomarkers in chronic viral hepatitis: A multicenter prospective study (the FIBROSTIC study)显示文摘 | Francoise Degos Paul Perez Bruno Roche Amel Mahmoudi Julien Asselineau Hélène Voitot Pierre Bedossa | 2010 | Journal of Hepatology2010，，6： | 3 |
| 5 | Self-compatibility in peach[Prunus persica(L.)Batsch]:patterns of diversity surrounding the S-locus and analysis of SFB alleles显示文摘Self-incompatibility(SI)to self-compatibility(SC)transition is one of the most frequent and prevalent evolutionary shifts in flowering plants.Prunus L.(Rosaceae)is a genus of over 200 species most of which exhibit a Gametophytic SI system.Peach[Prunus persica(L.)Batsch;2n=16]is one of the few exceptions in the genus known to be a fully selfcompatible species.However,the evolutionary process of the complete and irreversible loss of SI in peach is not well understood and,in order to fill that gap,in this study 24 peach accessions were analyzed.Pollen tube growth was controlled in self-pollinated flowers to verify their self-compatible phenotypes.The linkage disequilibrium association between alleles at the S-locus and linked markers at the end of the sixth linkage group was not significant(P>0.05),except with the closest markers suggesting the absence of a signature of negative frequency dependent selection at the S-locus.Analysis of SFB1 and SFB2 protein sequences allowed identifying the absence of some variable and hypervariable domains and the presence of additionalα-helices at the C-termini.Molecular and evolutionary analysis of SFB nucleotide sequences showed a signature of purifying selection in SFB2,while the SFB1 seemed to evolve neutrally.Thus,our results show that the SFB2 allele diversified after P.persica and P.dulcis(almond)divergence,a period which is characterized by an important bottleneck,while SFB1 diversified at a transition time between the bottleneck and population expansion. | Donia Abdallah Ghada Baraket Veronica Perez Amel Salhi Hannachi Jose I.Hormaza | 2020 | Horticulture Research2020，7，1： | 3 |
| 6 | Cytogenetic and Gene Expression Study in Radiation Workers Occupationally Exposed to Low Levels of Ionizing Radiation显示文摘Background:Exposure to IR(ionizing radiation)causes damage to living cells,especially to DNA,the degree of cellular damage depends on the radiation amount.Humans are naturally exposed to IR from cosmic rays,and artificially through diagnostic procedures,medical treatments or occupationally during work shifts.Cytogenetic assay of peripheral blood lymphocytes can provide a biological estimation of the dose received in IR exposures.The CBMN(cytokinesis-blocked micronucleus)assay is a widely used,since it represents a reliable test to detect radiation-induced chromosome aberration and it is a valuable biomarker in many biomonitoring studies on human populations occupationally exposed to IR.The NDI(nuclear division index)is a cell proliferation marker in cultures which is considered a measure of general cytotoxicity,the relative frequencies of the cells may be used to define cell cycles progression of the lymphocyte after mitogenic stimulation.Nevertheless the assay is frequently employed as a useful research tool for understanding the cell cycling kinetics of the cultures.Many gene expression studies demonstrated an up-regulation of genes involved in the processes of signal transduction,control of cell cycle,DNA repair and apoptosis after exposure of IR in different mammalian cell types.Altered expression of a few genes plays specific roles in DNA repair/cell cycle control such as CDKN1A,XPC,GADD45A,DDB2 and PCNA and cell cycle regulation/proliferation such as IL16,CABLES2,TGFB2 and RHOA.Objective:The present study aims to use the MN,NDI and gene expression analysis as biomarkers for investigation of the effects of IR exposure in some radiation workers occupationally exposed to low IR in Al-Tuwaitha site,Baghdad,Iraq.Also,authors assessed the effect of IR on the expression of some gene marker such as:RHOA,CDKN1A,GADD45A and RAD52.Methods:This study was carried out on thirty Iraqi male radiation workers occupationally exposed to low levels of IR at Al-Tuwaitha site,Baghdad,non-smokers and non-alcoholic,aged 30-59 years,as well as thirty apparently healthy individuals males collected randomly from population living in Baghdad,aged 30-59 years who are non-smokers,non-alcoholic as control group.Three molecular genetic parameters were employed such as CBMN,NDI and gene expression assay.The MN and NDI assay were performed according to the description by IAEA,2001.Total RNA was isolated from blood for radiation worker and control group.The RNA concentration was determined by measuring their absorbance that depends on the ratio A260/A280 of the wavelength,which leads to the determination of RNA purity,which ranged from 1.79-2.1 in two groups.Using RT-PCR for study gene expression,four types of specialized primer genes were selected for the mRNA genes:RHOA,CDKN1A,GADD45A and RAD52 which have a relation with IR in addition to the primers for internal control.Housekeeping gene((β-actin)was used as a reference gene to normalize the quantity of the target genes.Results:The results of cytogenetic analysis showed that micronuclei frequencies were significantly higher(p<0.01)in the radiation workers group in Al-Tuwaithasite,Baghdadas compared with the control group,and showed significant decrease(p>0.01)in the NDI in radiation workers as compared with the control group.Total RNA was isolated from blood for the radiation workers and control groups mentioned.Using RT-PCR for study gene expression,four types of specialized primer genes were selected for the mRNA:genes RHOA,CDKN1A,GADD45A and RAD52 which have a relation with IR in addition to the primers for internal control(β-actin)gene.The products of replicated specialized primers for the genes concerned and the cDNA for the studied samples were electrophoretically separated in agarose gels.The banding profiles were visualized by ethidium bromide staining,as the molecular weight were 135 bp,165 bp,185 bp and 470 bp(nitrogen-base pair)for RHOA,CDKN1A,GADD45A and RAD52 genes,respectively.Gene expression analysis revealed statistically significant(ΔΔCt comparative Ct method)transcriptional changes in three genes RHOA,GADD45A,CDKN1A up-regulated while the RAD52 gene down-regulated.Conclusions:The results indicated that there is a possibility of using the changes in the MN,NDI and genes expression such as RHOA,CDKN1A,GADD45A and RAD52 as useful biomarkers for detection of IR in radiation workers occupationally exposed to low levels of IR. | Abdulsahib.K.Ali.Al-Ziyad Amel J.Mutar Zainab Abbas Ouda Shatha K.Khyon | 2021 | Journal of US-China Medical Science2021，18，1： | 2 |
| 7 | Physiological, histopathological and cellular immune effects of Pergularia tomentosa extract on Locusta migratoria nymphs显示文摘The migratory locust Locusta migratoria(Orthoptera,Acrididae)is one of the most important pests due to its extensive and severe damage to crops in large parts of Africa and Asia.Biodegradable and ecologically natural products such as bota nical insecticides are emergi ng can didates for replaceme nt of usually applied chemical pesticides.The crude methanolic extract of Pergularia tomentosa(PME)was investigated for their toxicity and physiological aspects on L.migratoria nymphs.Results showed that treatment of newly emerged fourth and fifth instar nymphs resulted in significant mortality and significant repellent activity with an LC50 value of 0.18 and 0.38%,respectively,after seven days of treatment.The PME toxicity was also dem on strated by histopathological cha nges in the alime ntary canal resulting in con siderable disorganization and severe damage of the caeca and proventriculus structure.The extract induced cellular immune reactions which manifested by a significant decrease in the number of the differential haemocyte counts(prohemocytes and plasmatocytes)and important cell lysis.Data of biochemical analyses showed that the PME reduced the activity of acetylcholinesterase and induced the glutathione S-transferases.The neurotoxic effect was confirmed by the histological alterations in the brain structure,particularly in the neurosecretory cells showing typical signs of cell necrosis. | Meriam Miladi Khemais Abdellaoui Amel Ben Hamouda Iteb Boughattas Mouna Mhafdhi Fatma Acheuk Monia Ben Halima-Kamel | 2019 | Journal of Integrative Agriculture2019，18，12： | 2 |
| 8 | Geochemical characterization of the salinity of irrigated soils in arid regions(Biskra, SE Algeria)显示文摘The agriculture in Biskra,southeastern Algeria,is based on traditional practices and characterized by small irrigated fields.In the last decades,the increasing demand in water as well as the scarcity of rainfall has forced many farmers to use groundwater of low quality to maintain the profitability of their crops.Unfortunately,this practice seems to be the main harmful factor for soil quality in the region since it is responsible for the salinization of the irrigated areas.Aiming to assess the impact of this phenomenon,the soils of the irrigated perimeter of El Ghrous-a representative rural community located in the west of Biskra-have been analyzed.A set of 82 soil samples was collected from top and subsoil(0-15 and15-35 cm respectively),on which the following physicochemical analyzes were performed:Ca^(2+),Mg^(2+),K^(+),Na^(+),Cl^(-),SO_(4)^(2-),HCO_(3)^(-),NO_(3)^(-),pH,electrical conductivity(EC)and sodium adsorption ratio.A Principal Component Analysis was performed to individuate the geochemical processes that influenced significantly the evolution of soil salinity and its pathways.The results showed a calcium sulfate(CaSO_(4))facies with a high risk of salinity and a low to medium risk of alkalinity.The calcite residual alkalinity and generalized residual alkalinity decreased as the solutions became more concentrated.Most of the samples were oversaturated in carbonate minerals(aragonite,calcite,and dolomite)and under saturated in evaporitic minerals(anhydrite,gypsum,and halite).Finally,two multiple linear regressions(using cations and anions as independent variables)have been proposed to quantify soil salinity.These equations,with an accuracy of 85%,can represent a time and money-saving tool for managers and farmers to estimate the EC,in comparison to the traditional estimation methods. | Mohamed Amine Abdennour Abdelkader Douaoui Jesus Barrena Manuel Pulido Abdelhamid Bradai Amel Bennacer Chiara Piccini Alberto Alfonso-Torreno | 2021 | Acta Geochimica2021，40，2： | 2 |
| 9 | Coxiella burnetii,the causative agent of Q fever in Saudi Arabia:molecular detection from camel and other domestic livestock显示文摘Objective:To detect Coxiella burnetii(C.burnetii)DNA in clinical specimens from camel,goats,cattle and sheep in the Kingdom of Saudi Arabia.Methods:A total of 367 clinical samples including blood,milk,faeces and urine were collected from different livestock and subjected to PCR amplification using primers which amplify transposon-like region and transposase gene.Results:Positive amplification from both regions was obtained from camel,goats and cattle but not from sheep.A percentage of 10.8%samples yielded positive PCR amplification from both blood and milk,where 15 of 139 blood and 16 of 148 milk samples were positive.Faeces and urine showed higher percentages of positive samples reaching 40.8%and 23.8%respectively.Conclusions:The preferred route of shedding in camel appeared to be the faeces followed by urine,while that of goats appeared to be the faeces and that of the cattle appeared to be the milk. | Osama B.Mohammed Abdulrahman A.Jarelnabi Riyadh S.Aljumaah Mohammed A.Alshaikh Amel O.Bakhiet Sawsan A.Omer Abdulaziz N.Alagaili Mansour F.Hussein | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，9： | 2 |
| 10 | Association of hepatitis C virus infection and diabetes in central Tunisia显示文摘AIM:To investigate hepatitis C virus(HCV) seroprevalence in Tunisian patients with diabetes mellitus and in a control group.METHODS:A cross-sectional study was conducted to determine the HCV seroprevalence in 1269 patients with diabetes(452 male,817 female) and 1315 nondiabetic patients,attending health centers in Sousse,Tunisia.HCV screening was performed in both groups using a fourth-generation enzyme immunoassay.RESULTS:In the diabetic group,17(1.3%) were found to be HCV-infected compared with eight(0.6%) in the control group,although the difference was not significant(P=0.057).Quantitative PCR was performed in 20 patients.Eleven patients were positive and showed HCV genotype 1b in all cases.CONCLUSION:Frequency of HCV antibodies was low in patients with diabetes and in the control group in central Tunisia,with no signifi cant difference between the groups. | Naoufel Kaabia Elhem Ben Jazia Ines Slim Imen Fodha Wissem Hachfi Rafika Gaha Mabrouk Khalifa Aoutef Hadj Kilani Halim Trabelsi Ahmed Abdelaziz Fethi Bahri Amel Letaief | 2009 | World Journal of Gastroenterology2009，15，22： | 2 |
| 11 | Analysis of Genetic Diversity and Relationships in a Tunisian Fig (Ficus carica) Germplasm Collection by Random Amplified Microsatellite Polymorphisms显示文摘随机的放大微卫星多型性方法为土地用 18 教材联合跑的一套突尼斯的微量被进行。63 个随机的 amplifiedmicrosatellite 多型性标记的一个总数被获得并且过去常在这些栽培变种估计基因差异或检测贴错标签的盒子。数据证明设计过程组成阻止收音机暴露的一个吸引人、快速、便宜的方法。我们识别了在在这庄稼检测遗传性多态现象是很有效的教材联合。有算术一般水准 dendrogram 的导出的未加权的对组方法在陆地赛跑和存在之中显示出基因分叉一典型地连续性变异。在树的性之间的重要关联没被观察。用上述的标记,在陆地赛跑之间的歧视被完成。因此,随机的放大微卫星多型性被证明在描绘本地微量细菌原生质高度有效…… | Khaled Chatti Olfa Saddoud Amel Salhi-Hannachi Messaoud Mars Mohamed Marrakchi Mokhtar Trifi | 2007 | Journal of Integrative Plant Biology2007，49，3： | 2 |
| 12 | Magnetron SputterDg:a Review of Recent Developments and Applications显示文摘 | Kelly P J Amell R D | | 0，，03： | 1 |
| 13 | Free Abrasive Wear Behavior of UHMWPE Composites Filled with Wollastonite Fibers显示文摘 | Tong Jin Ma Yunhai Amell R D | 2006 | Composites Part A: Applied Science and Manufaturing2006，37，1： | 1 |
| 14 | Curent guidelines for the drug trentment of ankvlosing spondylitid 显示文摘 | Amell FC Dale DC Federman DD | 2004 | ACP Medicine2004，28，7： | 1 |
| 15 | Head and neck vancer due to heavy metal exposure via tobacco smoking and professional exposure 显示文摘 | Rim K Amel H C | 2010 | Toxicology and Applied Pharmacology2010，248，： | 1 |
| 16 | Recent Advances in Magnetron Sputte- ring 显示文摘 | Amell ll D kelly P J | 1999 | Surface & Coatings Technology1999，112，1： | 1 |
| 17 | Market structure and the pass-through of the federal funds rate显示文摘 | Robert M. Adams Dean F. Amel | 2010 | Journal of Banking and Finance2010，，5： | 1 |
| 18 | 2, 4- dichlorophenoxyacetic acid (2, 4-D ) sorption and degradation dynamics in three agricultural soils显示文摘 | BOIVIN A AMELLAL S SCHIAVON M | 2005 | Environ Pollut2005，138，1： | 1 |
| 19 | Recent advances in magnetron sputtering显示文摘 | AMELL R D KELLY P J | 1999 | Surface and Coatings Technology1999，112，1： | 1 |
| 20 | Electrolcss NiP micro- and nano-composite coatings 显示文摘 | SARRET M MULLER C AMELL A | 2006 | Surfacc Coating Technology2006，201，12： | 1 |
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