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| 1 | Vitamin D receptor expression in human bone tissue and dose-dependent activation in resorbing osteoclasts显示文摘The effects of vitamin D on osteoblast mineralization are well documented. Reports of the effects of vitamin D on osteoclasts, however, are conflicting, showing both inhibition and stimulation. Finding that resorbing osteoclasts in human bone express vitamin D receptor(VDR), we examined their response to different concentrations of 25-hydroxy vitamin D_3[25(OH)D_3](100 or 500 nmol·L^(-1)) and 1,25-dihydroxy vitamin D_3[1,25(OH)_2D_3](0.1 or 0.5 nmol·L^(-1)) metabolites in cell cultures. Specifically, CD14+ monocytes were cultured in charcoal-stripped serum in the presence of receptor activator of nuclear factor kappa-B ligand(RANKL) and macrophage colony-stimulating factor(M-CSF). Tartrate-resistant acid phosphatase(TRAP)histochemical staining assays and dentine resorption analysis were used to identify the size and number of osteoclast cells, number of nuclei per cell and resorption activity. The expression of VDR was detected in human bone tissue(ex vivo) by immunohistochemistry and in vitro cell cultures by western blotting.Quantitative reverse transcription-PCR(q RT-PCR) was used to determine the level of expression of vitamin D-related genes in response to vitamin D metabolites. VDR-related genes during osteoclastogenesis, shown by q RT-PCR, was stimulated in response to 500 nmol·L^(-1)of 25(OH)D_3and 0.1–0.5 nmol·L^(-1)of 1,25(OH)_2D_3,upregulating cytochrome P450 family 27 subfamily B member 1(CYP27B1) and cytochrome P450 family 24 subfamily A member 1(CYP24A1). Osteoclast fusion transcripts transmembrane 7 subfamily member 4(tm7sf4) and nuclear factor of activated T-cell cytoplasmic 1(nfatc1) where downregulated in response to vitamin D metabolites. Osteoclast number and resorption activity were also increased. Both 25(OH)D_3and1,25(OH)_2D_3reduced osteoclast size and number when co-treated with RANKL and M-CSF. The evidence for VDR expression in resorbing osteoclasts in vivo and low-dose effects of 1,25(OH)_2D_3on osteoclasts in vitro may therefore provide insight into the effects of clinical vitamin D treatments, further providing a counterpoint to the high-dose effects reported from in vitro experiments. | Allahdad Zarei Alireza Morovat Kassim Javaid Cameron P Brown | 2016 | Bone Research2016，4，3： | 5 |
| 2 | Distribution of PAHs and n-al-kanes in Klang River Surface Sediments,Malaysia显示文摘 | ALIREZA R B MOHAMAD P Z | 2010 | PertanikaJ Sci Technol2010，18，1： | 1 |
| 3 | In vitro cytotoxicity and phototoxicity of N-piperazinyl quinolone derivatives with a 2-thienyl group显示文摘 | Abbas P Alireza F Mehdi H | | 0，，： | 1 |
| 4 | Design of a general proposed neuro-fuzzy controller by using modified adaptive network based fuzzy inference sys- tem 显示文摘 | ALIREZA P ABDOLLAH K KHEYROLLAH H | 2010 | Int J Electron Commun2010，64，： | 1 |
| 5 | Characterization of Bovine Calpastatin Gene in Nelore Cattle Using Polymerase Chain Reaction - Restricted Fragment Length Polymorphisms 显示文摘 | ALIREZA M JOTHI M P AWIS Q S | 2009 | American Journal of Animal and Veterinary Sciences2009，4，4： | 1 |
| 6 | Ion-Exchanged Er/Yb Phosphate Glass Waveguide Amplifiers and Lasers显示文摘 | Alireza Shooshtari Peyman Meshkinfam Tahar Touam Mark Andrews P | | 0，，： | 1 |
| 7 | Application of membrane-coupled sequencing batch reactor for oilfield produced water recycle and beneficial reuse显示文摘 | Razi A F Alireza P Zurina Z A | 2010 | Bioresource Technology2010，101，18： | 1 |
| 8 | Insiders and the Law:The Impact of Regulatory Change on Insider Trading显示文摘 | ALIREZA T R TOMASE P W | 2004 | Research Paper Series Faculty of Business2004，，14： | 1 |
| 9 | Distribution of PAHs and n-alkanes in Kla ng R iver surface sediments, Malaysia显示文摘 | ALIREZA R B MOHAMAD P Z | 2010 | Pertanika Journal of Science & Technology2010，18，1： | 1 |
| 10 | Effect of frying process on fatty acid composition and iodine value of selected vegetable oils and their blends显示文摘 | ALIREZA S TAN C P HAMED M | 2010 | International Food Research Journal2010，17，： | 1 |
| 11 | A physicsbased coordinate transformationfor 3-D image matching显示文摘 | MALCOLM H D ALIREZA K DUANE P F | 1997 | IEEE transactions on medical imaging1997，16，3： | 1 |
| 12 | Realization of parasitics in stability of DC-DC converters loaded by constant power loads in advanced multiconverter automotive systems显示文摘 | Alireza Khaligh Chapman P Davoudi A | 2008 | IEEE Transactions on industrial electronics2008，55，6： | 1 |
| 13 | Benzocyelobutene in silicon:An integrated fabrication process for electrical and thermal isolation in MEMS显示文摘 | Alireza M Nima G Michael P | 2005 | Microelectronic Engineering2005，82，： | 1 |
| 14 | Regulation and efficiency &productivity considerations in water &wastewater industry:Case of Iran显示文摘 | Alireza E N Mohammad D Hamed P | 2014 | Procedia-Social and Behavioral Sciences2014，109，1： | 1 |
| 15 | Embedded Benzocyclobutene in Silicon:An Integrated Fabrication Process for Electrical and Thermal Isolation in MEMS显示文摘 | Alireza W Nima G Michael P | 2005 | Microelectron Eng2005，82，2： | 1 |
| 16 | Energy control of super capacitor/fuel cell hybrid power source显示文摘 | Alireza P Serge P Farid M T | 2008 | Energy Conversion and Management2008，，49： | 1 |
| 17 | Modeling of CCR5antagonists as anti HIV agents using combined genetic algorithm and adaptive neuro-fuzzy inference system(GA-ANFIS)显示文摘 | MOHSEN S ALIREZA P | 2013 | Med Chem Res2013，22，1： | 1 |
| 18 | How after-sales service quality dinmensions affect customer satisfaction显示文摘 | ALIREZA F FATEMEH B PEGAH M P | 2011 | African Journal of Business Management2011，5，17： | 1 |
| 19 | Effect of frying process on fatty acid composition and iodine value of selected vegetable oils and their blends显示文摘 | ALIREZA S TAN C P HAMED M | 2010 | International food research journal2010，17，： | 1 |
| 20 | Application of membrane-coupled sequencing batch reactor for oilfield produced water recy- cle and beneficial reuse 显示文摘 | Fakhru'l-Razi A Alireza P Zurina Z A | 2010 | Bioresource Tech- nology2010，101，18： | 1 |
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