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| 1 | Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma显示文摘AIM To establish the role of vascular endothelial growth factor (VEGF) in the oncogenesisof human gastric carcinoma more directly.METHODS The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF165 complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or downregulated.RESULTS VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR,localized in both the cytoplasm and membrane.Introduction of VEGF165 antisense into human gastric cancer cells ( SGC-7901, immunofluorescence intensity,31.6%)) resulted in a significant reduction in VEGFspecific messenger RNA and total and cell surface VEGF protein ( immunofluorescence intensity, 8.9%)(P<0.05). Conversely, stable integration of VEGF165 in the sense orientation resulted in an increase in cellular and cell surface VEGF (immunofluorescence intensity,75.4%) (P<0.05). Lowered VEGF levels were associated with a marked decrease in the growth of nude mouse xenografted tumor (at 33 days postimplantation, tomor volume: 345.40 ± 136.31 mm3) (P<0.05 vs control SGC7901 group: 1534.40 ± 362.88 mm3), whereas up-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tomor volume: 2350.50 ± 637.70mm3) (P<0.05 vs control SGC-7901 group).CONCLUSION This study provides direct evidence that VEGF plays an important role in the oncogenesis of human gastric cancer. | Du-Hu Liu Xue-Yong Zhang Dai-Ming Fan Yu-Xin Huang Jin-Shan Zhang Wei-Quan Huang Yuan-Qiang Zhang Qing-Sheng Huang Wen-Yu Ma Yu-Bo Chai Ming Jin Institute of Digestive Disease,Xijing Hospital,~2 Department of Gastroenterology,Tangdu Hospital,~3Department of Histology and Embryology,~4 Department of Microbiology,~5 Department of Biochemistry,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 37 |
| 2 | Overexpression of p27^(KIP1)induced cell cycle arrest in G_1 phase and subsequent apoptosis in HCC-9204 cell line显示文摘AIM We have previously reported that inducibleover-expression of Bak may prolong cell cycle inG1 phase and lead to apoptosis in HCC-9204 cells.This study is to investigate whether p27KIP1playsan important role in this process.METHODS In order to elucidate the exactfunction of p27KIP1in this process,a zinc induciblep27KIP1stable transfectant and transient p27KIP1-GFP fusion transfectant were constructed.Theeffects of inducible,p27KIP1on cell growth,cellcycle arrest and apoptosis were examined in themock,control pMD vector,and pMD-KIP1transfected HCC-9204 cells.RESULTS This p27KIP1-GFP transfectant maytransiently express the fusion gene.The cellgrowth was reduced by 35% at 48 h of p27KIP1induction with zinc treatment as determined bytrypan blue exclusion assay.These differencesremained the same after 72 h of p27KIP1expression,p27KIP1caused cell cycle arrest after24 h of induction,with 40% increase in G1population.Prolonged p27KIP1expression in thiscell line induced apoptotic cell death reflected byTUNEL assay.Fourty-eight h and 72 h of p27KIP1expression showed a characteristic DNA ladder onagarose gel electrophoresis. CONCLUSION Bak may induce cell cycle arrest inG1 phase through upregulating expression ofp27KIP1and subsequently lead to apoptosis inHCC-9204 cells.The p27KIP1-GFP fusion proteincan be transiently expressed in HCC-9204 cells.The inducible p27KIP1-expressing cell line providesa model to assess p27KIP1function. | Jiang Li Xin Ke Yang Xin Xin Yu Meng Liang Ge Wen Liang Wang Jie Zhang Yun De Hou Department of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China State Key Laboratory for Molecular Virology and Genetic Engineering,Beijing 100052,China Department of Dermatology,Beijing Hospital,Beijing 100016,China Institute of Radiation Medicine,Beijing 100085,China | 2000 | World Journal of Gastroenterology2000，6，4： | 20 |
| 3 | Extraction and purification of TGFβ and its effect on the induction of apoptosis of hepatocytes显示文摘AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine platelets by acid/ethanol extraction method and purified with ion exchange and gel chromatography. The extracted TGF β was injected subcutaneously to mice, and its biological activity in vivo was observed 72 hfs post-injection by HE staining. The morphological changes were observed by HE staining and the occurrence of apoptosis was detected by TUNEL method after the human normal hepatic cell line QZG was treated with 8 μg@L 1 TGFβ for 12 hrs in vitro.RESULTS The molecular mass 25 ku TGF β protein was successfully extracted. It was able to induce localized granulation tissue formation in vivo. TGF β-treated hepatocytes showed obvious apoptotic morphological changes, including the pyknosis and dense-stained nuclei and cytoplasm, the fragmentary, annular or crescent nuclei, and the 'bubbling' cytoplasm. Moreover, its apoptotic rate was significantly higher than that of the control group (P<0.05).CONCLUSION Biological active TGF β protein is extracted and purified successfully from bovine platelets, and it is able to induce the apoptosis of hepatocytes. | Xiao-Hui Si Lian-Jun Yang Research Institute of Stomatology,the Ninth People’s Hospital,Shanghai Second Medical University,Shanghai 200011,ChinaDepartment of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 15 |
| 4 | Pharmacokinetics of radioimmunotherapeutic agent of direct labeling mAb ^(188)Re-HAb18显示文摘AIM: To labed Anti-hepatoma monoclonal antibody (mAb)fragment HAb18 F (ab')2 was labeled with 188 Re for thepharmacokinetic model of 188 Re-HAb18 F (ab')2 and toevaluate its pharmacokinetic parameters in hepatoma-bearing nude mice.METHODS: HAb18 F(ab')2 was directly labeled with 188Reusing 2-mercaptoethanol (2-ME) as reducing agents.Labeling efficiency and immunoreactivity of 188 Re-HAb18 F( ab')2 were evaluated by Whatman 3MM paperchromatography and live cell assay, respectively.Biodiatribution analysis was also conducted in nude micebearing human hepatoma in which animals were sacrificed atdifferent time points(1, 4, 18, 24 and 24h) after 188Re-HAb18F(ab' )2 was injected through tail-vein into hepatoma-bearingnude mice. The blood and radioactivity of organs and masswere measured. The concentrations of 188 Re-HAb18 F(ab')2were evaluated with a pharmacokinetic 3P97 software.RESULTS: The optimum labeling efficiency andimmunoreactive fraction were 91.7% and 0.78%,respectively. The parameters of 188Re-HAb18 F(ab')2 were:T1/2, 2.29h; Vd, 1.49 × 10-9L@ Bq- 1; AUC, 20.49 × 109Bq@ h@L-1 ;CL, 0.45 × 10-3L@ h- 1. 188Re- HAb18 F(ab')2 could locatespecially in hepatoma with high selective reactivity of HAb18F(ab')2. 188 Re-HAb18 F(ab')2 was mainly eliminated bykidney. The maximal tumor to blood ratio was at 48h, andmaximal tumor to liver ratio was at 18h.CONCLUTION: The pharmacokinetics of 188Re-HAb18 F(ab')2fit a I-compartment model. 188 Re-HAb18 F(ab')2 can beuptaken selectively at the hepatoma site. | Chao Lou Zhi-Nan Chen Hui-Jie Bian Department of Cell Engineering Research Centre,Jie Li,Department of Oral Cell Biology,Qingdu Hospital,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China Shou-Bo Zhou School of Biological Sciences,University of Manchester,Oxford Road,United Kingdom | 2002 | World Journal of Gastroenterology2002，8，1： | 14 |
| 5 | Identification of the epitopes on HCV core protein recognized by HLA-A2 restricted cytotoxic T lymphocytes显示文摘AIM To identify hepatitis C virus (HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL).METHODS Utilizing the method of computer prediction followed by a 4 h 51 Cr-release assay confirmation.RESULTS The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide 'ALAHGVFAL (core TS0-158)'.The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%,respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis.But blocking of CTL response with anti-CD8 mAb could abolish the Iysis.CONCLUSION The peptide (core 150 - 158 ) is the candidate epitope recognized by HLA-A2 restricted CTL. | Hong-Chao Zhou De-Zhong Xu Xue-Ping Wang Jing-Xia Zhang Ying-Huang Yong-Ping Yan Yong Zhu Bo-Quan Jin Department of Epidemiology,the Fourth Military Medical University,Xi’an 710033,Shaanxi Province,ChinaDepartment of Immunology,the Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 11 |
| 6 | Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells显示文摘AIM: To investigate the expression level of ZNRD1 gene in gastric cancer cells SGC7901 and gastric cancer MDR (multidrug resistant) cells SGC7901/VCR, and to observe the drug sensitizing and proliferation effect of ZNRD1 antisense nucleic acid transduction on SGC7901/VCR cells.METHODS: Amplification of sequences encoding ZNRD1 from SGC7901/VCR cDNA by PCR. The levels of ZNRD1 mRNA expression were demonstrated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR).Eukaryotic expression vector pcDNA3.1-anti ZNRD1 was constructed and transfected into SGC7901/VCR cells by lipofectamine. Immunochemical method was used to detect the expression of protein in SGC7901/VCR cells and transfectants. The cell cycle alteration and the intracellular adriamycin (ADM) accumulation were observed by FACS.Growth curve and drug sensitization of cells for vincristine (VCR) were analyzed with MTT assay.RESULTS: We cloned the open reading frame of full-length ZNRD1. The expression of ZNRD1 showed higher in SGC7901/VCR than in SGC7901 cells. The antisense ZNRD1 drug-resistant clones were selected after gene transfection.Immunochemical results showed that the expression level of ZNRD1 protein was lower in anti ZNRD1-SGC7901/VCR cells than that in non-transfectants. Comparing to SGC7901/VCR and pcDNA3.1-SGC7901/VCR, anti ZNRD1-SGC7901/VCR showed gradually accumulated in G1 phase, with aconcomitant decrease of cell population in S phase. FACSalso suggested intracellular ADM accumulation increased2fold in SGC7901/VCR cells after transfected with antisenseZNRD1.MTT assay showed that transfectant cells proliferationwas lagged and more sensitive to vcr than non-transfectants.CONCLUSION: ZNRD1 gene displayed highly expression in VCR resistant gastric cancer cells.Expression of ZNRD1 protein was effectively blockde in anti ZzNRD1-SGC7901/VCR cells by gene transfection.ZNRD1 antisense nucleic acid transfection sensitized drug resistant gastric cancer cells to VCR,increased ADM accumulation and inhibited the cells to VCR,incereased ADM accumulation qand inhibited the cells proliferation.ZNRS1 antisense RNA transduction could reverse the MDR of human drug-resistant gastric cancer cell SGC7901/VCR to a degree. | Yu-Mei Zhang Yan-Qiu Zhao Yang-Lin Pan Yong-Quan Shi Xiao-Hang Jin Hui Yi Dai-Ming Fan Department of Gastroenterology,Xijing Hospital,the Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，5： | 7 |
| 7 | Cloning of differentially expressed genes in human hepatocellular carcinoma and nontumor liver显示文摘INTRODUCTIONThe mechanism of hepatocellular carcinoma(HCC)is still unclear,although some genes have been found to play a role in the transformation of liver cells,and a variety of studies have described differences in gene expression which distinguished tumor from nontumor[1-6].The new genes,especially the functional genes directly related with tumor are still worth being found.The purpose of our study is to find the different genes between human liver tumor and normal tissues using suppression subtractive hybridization. | Xiao-Yan Cao Jie Liu Zhao-Rui Lian Marcy Clayton Jia-Lu Hu Ming-Hua Zh Dai-Ming Fan Mark Feitelson Institute of Digestive Diseases,Xijing Hospital,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,ChinaDepartment of Pathology & Cell Biology,Thomas Jefferson University,Philadelphia,PA19107 USADepartment of Pathology,Second Military Medical University,Shanghai 200433,China | 2001 | World Journal of Gastroenterology2001，7，4： | 7 |
| 8 | Inhibition of HBV targeted ribonuclease enhanced by introduction of linker显示文摘AIM: To construct human eosinophil-derived neurotoxin (hEDN) and HBV core protein (HBVc) eukaryotic fusion expression vector with a linker (Gly4 Ser)3 between them to optimize the molecule folding, which will be used to inhibit HBV replication in vitro.METHODS: Previously constructed pcDNA3. 1(-)/TR was used as a template. Linker sequence was synthesized and annealed to form dslinker, and cloned into pcDNA3.1(-)/TR to produce plasmid pcDNA3.1(-)/HBc-linker. Then the hEDN fragment was PCR amplified and inserted into pcDNA3.1(-)/HBc-linker to form pcDNA3.1(-)/TNL in which the effector molecule and the target molecule were separated by a linker sequence. pcDNA3.1(-)/TNL expression was identified by indirect immunofluorescence staining. Radioimmunoassay was used to analyse anti-HBV activity of pcDNA3.1(-)/TNL.Meanwhile, metabolism of cells was evaluated by NTT colorimetry.RESULTS: hEDN and HBVc eukaryotic fusion expression vector with a linker (Gly4Ser)3 between them was successfully constructed. pcDNA3.1(-)/TNL was expressed in HepG2.2.15 cells efficiently. A significant decrease of HBsAg concentration from pcDNA3.1(-)/TNL transfectant was observed compared to pcDNA3. 1(-)/TR (P=0.036, P<0.05).MTT assay suggested that there were no significant differences between groups (P=0.08, P>0.05).CONCLUSION: Linker introduction enhances the inhibitory effect of HBV targeted ribonuclease significantly. | Wei-Dong Gong Jun Liu Jin Ding Ya Zhao Ying-Hui Li Cai-Fang Xue Department of Pathogenic Organisms,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，7： | 6 |
| 9 | APPLICATION OF THE METHODS OF NONLINEAR DYNAMICS TO HEART RATE VARIABILITY ANALYSIS显示文摘APPLICATIONOFTHEMETHODSOFNONLINEARDYNAMICSTOHEARTRATEVARIABILITYANALYSISAPPLICATIONOFTHEMETHODSOFNONLINEARDYNAMICSTOHEARTRATE... | Ming Xin Qin Ke Ying(Department of Biomedical Engineering,Fourth Military Medical University, Xi’ an 710033 China) | 1995 | Chinese Journal of Biomedical Engineering(English Edition)1995，4，3： | 2 |
| 10 | Hyperbaric oxygen preconditioning induces tolerance against spinal cord ischemia in rabbits显示文摘 | DONG Hai Long XIONG Li Ze ZHU Zheng Hua Department of Anesthesiology Xijing Hospital Fourth Military Medical University Xi’an 710033 China | 2000 | 第四军医大学学报2000，，04： | 1 |
| 11 | Ultra-microstructural changes in iliac artery after bare and magnetic stent implantation in rabbits显示文摘Objective To investigate the preventive effect of magnetic stent on coronary restenosis after percutaneous arterial stenting. Methods Twenty rabbits were divided randomly into 2 groups.Bare stent (BS group,n=10) or magnetic stent (MS group,n=10) was implanted in the left iliac artery of the rabbits of the 2 groups,respectively.Aspirin (25rag,qd) was administered orally to the rabbits of both groups from 3 days before stenting until the rabbits were executed.Unfractionated heparin (2500u,qd) was delivered subcuta- neously after stenting for 7 days.Five rabbits of each group were randomly selected to be executed at 7 or 30 days.Structural changes in the injured arteries were studied by optical microscopey,transmissive electronic microscopey and immunohistochemistry.Results At 7 days,more myofibroblasts were found migrating from adventitia to tunica media and intima in BS group than in MS group.Inside the media and intima,large amount of smooth muscle cells of synthetic type were observed.At 30 days after stenting,in magnetic group, most uascular smooth muscle cells (SMCs) under the intima had transformed to contractile type and only little extracellular matrix (ECM) was observed around the SMCs;whereas,in BS group,the SMCs remained to be synthetic type and large amount of ECM was observed around the SMCs,which was composed mainly ofproteoglycans and glycoproteins.Conclusions Magnetic stent can inhibit proliferation and migration of SMCs and reducing the production of ECM,and therefore,may prevent restenosis after coronary stenting. | Xinhong Guo~1 Guoliang Jia~2 Anlin Lu~2 Xinguo Zhao~2 Fei Li~2 Rongqing Zhang~2 1 Institute of Geriatric Cardiology, Chinese PLA General Hospital, Beijing 100853, China 2 Department of Cardiology, Xijing Hospital, Xi’an 710033, China | 2008 | Journal of Geriatric Cardiology2008，5，3： | 0 |
| 12 | Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridi-zation and cDNA library array显示文摘To isolate the over-expressed genes in human renal cell carcinoma (RCC) and analyze its molecular basis of carcinogenesis, we used the mRNA from human RCC tissues as tester and that from the matched normal kidney tissues as driver to construct the suppression subtractive hybridization library. 379 of the subtracted clones were arrayed onto a nylon mem-brane and the over-expressed genes were then screened by hybridizing the filter with radioac-tively labeled cDNA from RCC and matched normal kidney tissues. 67 clones over-expressed in RCC by a factor of 6 or more were sequenced and its identities were analyzed in GenBank da-tabase. 4 clones were previously unknown fragments and 2 clones represent KIAA genes. The rest clones were the known genes and some of them were RCC-related, including vascular en-dothelial growth factor, vimentin and tissue factor. Most of the known genes were the RCC-related genes previously unknown, including zinc ribbon domain-containing 1 protein (ZNRD1), pituitary tumor transforming gene1 (PTTG1). Northern blot and semi-quantitative RT-PCR confirmed that the mRNA levels of the 3 novel fragments and 1 KIAA and 3 known genes were significantly higher in RCC than in the matched normal kidney tissues. Immunohis-tochemical and Western blot analysis for PTTG1 and ZNRD1 revealed increased protein level in RCC. The over-expressed genes in RCC are the potential molecular targets for diagnosis and therapy and it is very important to understand the molecular mechanism of RCC through the profile of over-expressed genes. | AI Junkui1, ZHANG Zhiwen2, XIN Dianqi1, ZHU Hongjian1, YAN Quanjian3, XIN Zhongcheng1, NA Yanqun1 & GUO Yinglu1 1. Institute of Urology, Peking University & Department of Urology, First Hospital, Peking University, Beijing 100034, China 2. Department of Physiology, Peking University Health Science Center, Beijing 100083, China 3. Department of Urology, Xijing Hospital, the 4th Military Medical University, Xi抋n 710033, China | 2004 | Science China(Life Sciences)2004，47，2： | 0 |
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