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| 1 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 2 | A study on zooplankton distribution patterns and indicator species in Kuroshio upstream area and adjacent East China Sea显示文摘On the basis of the data of zooplankton biomass and three major taxa—— Copepoda, Chaetognatha andSiphonophora of May-June 1986, July-August and December 1987, the distributional patterns and the indicator species of zooplankton in the Kuroshio and adjacent waters of the East China Sea are preliminarily studied. The results are as follows:The horizontal distribution of zooplankton biomass and the abundance of copepods, chaetognaths and siphonophores arecurred in the continent area northwest of Taiwan and the south-centre section of the East China Sea continent, which are the mix front of different waters. Zooplankton in the water area inside of Ryukyu Islands presented low abundance and high diversity. There are clear seasonal variations in zooplankton biomass and abundance in the study area. The strength or weakness of different water masses and fronts is the basic reason for the variations of zooplankton biomass and abundance.The species composition of zooplankton in the study area is complex and | He Dehua, Wang Chunsheng, Liu Hongbin and Yang Guanming( Received July 9, 1990 accepted November 12, 1990) | 1992 | Acta Oceanologica Sinica1992，11，2： | 4 |
| 3 | A Study of the Characteristics of Consumers' Behavior under the Web Environmen显示文摘The development of Internet has made shopping online possible. But while we are focusing ourselves on the environment design, plan for the information structures and items or business scales, another important factor has been ignored, that is, the changes of the characteristics of consumers behavior under the web environment. Because we are not able to make the exact online marketing strategy according to the changes of consumers behavior, and although many online shops have been opened, we are not able to get big sales. This article emphasises the analysis of the environment for on line transactions, attampts to investigate the basic modes for information movement and on line shopping of those consumers under the web environment, study the influence on consumers brought by Internet, the shopping styles and their characteristics. | LIU Li (Department of Management Engineering, Nanjing University of Posts and Telecommunications, Nanjing 210003, P.R.China)Biography:\ LIU Li (1958-), male, from Hunan Province, received MS degree in management from Northeast University in 1990, associ | 2001 | The Journal of China Universities of Posts and Telecommunications2001，8，1： | 3 |
| 4 | Palaeoceanological significance of Holocene ostracean banks in Tianjin area显示文摘On the basis of the study of ostracean banks in Tianjin area, the present study recognized that the ostracean banks at Ninghe County in Tianjin area are well developed along the west coast of the Bohai Sea; they consist of thanato-coenose and taphocoenose from Crassistrea gigars and C. revularis. From the 14C datings it can be known that the banks had formed from early Middle-Holocene and continued up to over 2 ka BP (14C age). The top has different reliefs . Although there is an effect of spatial shift of estuary position, the top is only distributed in the range of-0. 50--2. 50 m(Huanghai elevation minus National elevation system). The banks cannot explain the sea-level fluctuation. As the string ostracean banks are distributed near E-W direction, it suggests that the direction of tidal currents was then similar to that of the present day. | Wang Qiang, Li Xiuwen, Zhang Zhiliang, Li Fenglin, Shao Jun, Bai Dezhong, Shang Xuexi and Tang Ruolu(Received October 24, 1990 accepted November 14, 1990) | 1991 | Acta Oceanologica Sinica1991，10，2： | 2 |
| 5 | 未来20年中医药研究发展预测(摘要)显示文摘采用美国兰德公司创造的特尔斐(Delphi)预测技术,对未来20年(1990~2010)内中医药在基础理论、临床、中药、针灸、气功推拿及情报、文献、医史方面可能出现的重大进展及突破进行了预测。 | '1990~2010年中医药研究的重大进展与突破预测研究'课题组 | 1992 | 山东中医学院学报1992，16，1： | 2 |
| 6 | Several noteworthy higher fungi from southem Yunnan, China 显示文摘 | Yang ZL 1990 | 1990 | Mycotaxon1990，38，： | 1 |
| 7 | The Interdependence Between Ownership Status and Market Structure:The Case of Privatization显示文摘 | Fershtman 1990 | 1990 | Economica1990，，： | 1 |
| 8 | DNA polymorphism amplified polymorphic by arbitrary primers are useful as genetic markers显示文摘 | Williams JGK Kubelik AR Livak KJ 1990 | 1990 | Nucleic Acids Research1990，18，： | 1 |
| 9 | Criteria for diagnosis of Behcet's disease显示文摘 | International Study Group for Behcet's Disease (1990) | 1990 | Lancet1990，335，8697： | 1 |
| 10 | Storage of seeds: Potential and limitation for germplasm conservation 显示文摘 | Bonner FT 1990 | 1990 | Forest Ecolog Manag1990，35，： | 1 |
| 11 | Generation of alien chromosome addition lines from synthetic Brassica napes: morphology, cytology, fertility, and chromosome transmission显示文摘 | McGrath J M Quiros C E 1990 | 1990 | Genome1990，33，： | 1 |
| 12 | Attractiveness as a tourist destination: A comparative study of Thailand and selected countries显示文摘 | Tang J Roehananond N(1990) | 1990 | Socio-Economic Planning Sciences1990，，： | 1 |
| 13 | 冠心病中医辨证标准仁显示文摘 | 中国中西医结合学会心血管学会1990年10月修订 | 1991 | 中国中西医结合杂志1991，11，5： | 1 |
| 14 | Cross -subsidization and Cost Misallocation by Regulated Monopolists显示文摘 | Brennan 1990 | 1990 | Journal of Regulatory Economics1990，3751，： | 1 |
| 15 | Financial Development,Growth,and the Distribution of Income显示文摘 | Greenwood Jeremy and Boyan Jovanovic (1990) | 1990 | Journal of Political Economy1990，98，5： | 1 |
| 16 | Ontogeny of an Abaenopsis raciboskii wolosz and its taxonomical relevance显示文摘 | Xing S D Zhou L E 1990 | 1990 | Acta Phytotaxonomica Sinica1990，28，5： | 1 |
| 17 | Structure and properties of porous hypercrosslinked polystyrene-the first representative of a new class of polymer networks显示文摘 | Davankov V A Tsyurupa M P 1990 | 1990 | React Polym1990，13，1： | 1 |
| 18 | Strategy,Social Responsibility and Implementation显示文摘 | Kraft Kenneth and Hage Jerald 1990 | 1990 | Journal of Business Ethics1990，9，： | 1 |
| 19 | Epigeic lichen communities of taiga and tundra regions显示文摘 | Ahti T Oksanen J 1990 | 1990 | Vegetatio1990，86，： | 1 |
| 20 | A Design for a process-approach writing course 显示文摘 | Keh Claudia L 1990 | 1990 | English Teaching Forum1990，，10： | 1 |
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