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| 1 | Linking hydraulic properties of fire-affected soils to infiltration and water repellency显示文摘 | John A. Moody David A. Kinner Xavier úbeda | 2009 | Journal of Hydrology2009,,3: | 1 |
| 2 | P11.08: Three‐dimensional ultrasound in diagnosis of septate uterus in patients with suspected congenital uterine anomalies显示文摘 | B.Graupera M.Pascual B.úbeda L.Hereter C.Pedrero I.Rodriguez | 2010 | Ultrasound Obstet Gynecol2010,,: | 1 |
| 3 | Physiologic changes in homocysteine metabolism in pregnancy: A longitudinal study in Spain显示文摘 | Natalia úbeda Leticia Reyes Antonio González-Medina Elena Alonso-Aperte Gregorio Varela-Moreiras | 2011 | Nutrition2011,,9: | 1 |
| 4 | Immunophenotype distinction between acute promyelocytic leukaemia and CD15? CD34? HLA‐DR? acute myeloid leukaemia with nucleophosmin mutations显示文摘 | Angela Ferrari Elena Bussaglia Josep úbeda Luca Facchini Anna Aventin Jorge Sierra Josep F. Nomdedéu | 2011 | Hematol Oncol2011,,3: | 1 |
| 5 | Manipulation of bicarbonate concentration in sperm capacitation media improves in vitro fertilisation output in porcine species显示文摘Background: The in vivo concentration of bicarbonate(HCO3-), one of the essential sperm capacitating effectors,varies greatly in the different environments sperm go through from cauda epididymis to the fertilisation site. On the contrary, porcine in vitro sperm capacitation and fertilisation media usually contains a standard concentration of25 mmol/L, and one of the main problems presented is the unacceptable high incidence of polyspermy. This work hypothesised that by modifying the HCO3-concentration of the medium, the output of in vitro sperm capacitation and fertilisation could be increased.Results: Once exposed to the capacitation medium, the intracellular pH(pHi) of spermatozoa increased immediately even at low concentrations of HCO3-, but only extracellular concentrations of and above 15 mmol/L increased the substrates protein kinase A phosphorylation(pPKAs). Although with a significant delay, 15 mmol/L of HCO3-stimulated sperm linear motility and increased other late events in capacitation such as tyrosine phosphorylation(Tyr-P) to levels similar to those obtained with 25 mmol/L. This information allowed the establishment of a new in vitro fertilisation(IVF)system based on the optimization of HCO3-concentration to 15 mmol/L, which led to a 25.3% increment of the viable zygotes(8.6% in the standard system vs. 33.9%).Conclusions: Optimising HCO3-concentrations allows for establishing an IVF method that significantly reduced porcine polyspermy and increased the production of viable zygotes. A concentration of 15 mmol/L of HCO3-in the medium is sufficient to trigger the in vitro sperm capacitation and increase the fertilisation efficiency in porcine. | Cristina Soriano-úbeda Jon Romero-Aguirregomezcorta Carmen Matás Pablo E.Visconti Francisco A.García-Vázquez | 2019 | Journal of Animal Science and Biotechnology2019,10,2: | 1 |
| 6 | Reproductive fluids,used for the in vitro production of pig embryos,result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM显示文摘Background:In vitro embryo production(IVP)and embryo transfer(ET)are two very common assisted reproductive technologies(ART)in human and cattle.However,in pig,the combination of either procedures,or even their use separately,is still considered suboptimal due to the low efficiency of IVP plus the difficulty of performing ET in the long and contorted uterus of the sow.In addition,the potential impact of these two ART on the health of the offspring is unknown.We investigated here if the use of a modified IVP system,with natural reproductive fluids(RF)as supplements to the culture media,combined with a minimally invasive surgery to perform ET,affects the output of the own IVP system as well as the reproductive performance of the mother and placental molecular traits.Results:The blastocyst rates obtained by both in vitro systems,conventional(C-IVP)and modified(RF-IVP),were similar.Pregnancy and farrowing rates were also similar.However,when compared to in vivo control(artificial insemination,AI),litter sizes of both IVP groups were lower,while placental efficiency was higher in AI than in RF-IVP.Gene expression studies revealed aberrant expression levels for PEG3 and LUM in placental tissue for C-IVP group when compared to AI,but not for RF-IVP group.Conclusions:The use of reproductive fluids as additives for the culture media in pig IVP does not improve reproductive performance of recipient mothers but could mitigate the impact of artificial procedures in the offspring. | E.París-Oller S.Navarro-Serna C.Soriano-Úbeda J.S.Lopes C.Matás S.Ruiz R.Latorre O.López-Albors R.Romar S.Cánovas P.Coy | 2021 | Journal of Animal Science and Biotechnology2021,12,3: | 1 |
| 7 | A new device for deep cervical artificial insemination in gilts reduces the number of sperm per dose without impairing final reproductive performance显示文摘Background: The aim of this study was to evaluate the reproductive performance of a new artificial insemination(AI) device specifically designed for gilts(Deep cervical AI, Dp-CAI) by means of which the sperm is deposited deeply in the cervix(8 cm more cranial than in traditional cervical insemination-CAI). New AI techniques have arisen in recent decades in the porcine industry, such as post-cervical artificial insemination(PCAI), which involves depositing the sperm in the body of the uterus [through a catheter(outer tube)-cannula(inner tube)] rather than by CAI. Although the PCAI method has been successfully applied in farm conditions to reduce sperm doses without impairing the reproductive performance, this technique has limitations in gilts mainly because of the difficulty involved in introducing the inner cannula through the cranial part of the cervix. For this reason, the Dp-CAI method described herein may be considered as an alternative to CAI and PCAI methods in gilts.Results: Gilts were divided in two experimental groups: 1) Dp-CAI: gilts(n = 1166) inseminated using 1.5 × 109 sperm/45 m L;2) CAI(as a control group): gilts(n = 130) inseminated using 2.5 × 10~9 sperm/85 mL. The Dp-CAI method was successfully applied in 88.90% of the gilts, with no differences detected between gilts with 1 or 2 previous oestrus cycles, although the catheter could be introduced more deeply in 2 oestrus gilts(P < 0.05). As the length of the insemination device that could not be introduced increased(at the moment of insemination), so the success rate of the Dp-CAI device fell, as did the total number of piglets born. When the reproductive output in CAI and Dp-CAI was compared, none of the parameters analysed [pregnancy and farrowing rates(%), and number of piglets born(total and live)] showed significant differences.Conclusions: The use of the Dp-CAI technique provides a new AI method as an alternative to CAI and PCAI for pigs. The device, especially designed for gilts, was used with a high degree of success reducing conventional sperm doses without impairing reproductive parameters. | Pedro J.Llamas-López Rebeca López-úbeda Gustavo López Emily Antinoja Francisco A.García-Vázquez | 2019 | Journal of Animal Science and Biotechnology2019,10,2: | 0 |
| 8 | Hydration status,body composition,and anxiety status in aeronautical military personnel from Spain:a crosss-ectional study显示文摘Background: An adequate hydration status is critical to ensure efficiency during mental and physical activities.Our goal was to assess the hydration status of a Spanish group of aeronautical military men and to determine the association of hydration status with body composition and anxiety.Methods: A total of 188 men were evaluated through a validated hydration questionnaire, anthropometric and biochemical parameters, and an anxiety questionnaire. Based on these methods, the criteria of hydration were established.Results: Of the total sample, 81% met the hydration criteria(urine color = well hydrated, water balance ≥0 ml, and total water intake/weight ≥35 ml/kg), and 19% did not meet the hydration criteria(urine color = not sufficiently hydrated or dehydrated, water balance <0 ml, and total water intake/weight <35 ml/kg). Subjects not meeting the hydration criteria had lower urine pH, negative water balance, and lower water intake. The latter also had higher anxiety status(score=4 vs. 3, P=0.026), weight [(84.7±10.5) kg vs.(80.5±10.2) kg], body mass index [(26.3±3.1) kg/m;vs.(25.2±2.8) kg/m;], body fat [(22.3±5.6)% vs.(18.3±6.5)%], urine specific gravity, and urine color. Using a logistic binary regression model, hydration status was related significantly with the percentage of body fat(P=0.004), but no relation was found with age, comorbidities, or medications. Furthermore, total water intake/weight was positively correlated with percentage of body water(r=0.357, P=0.000) and negatively with body fat(kg)(r=–0.427, P=0.000), percentage of body fat(r=–0.405, P=0.000), and waist/hip ratio(r=–0.223, P=0.002). Based on a linear regression model, total water intake/weight was related significantly with percentage of body fat(P=0.001) and percentage of body water content(P=0.035). No relation was found, however, with waist/hip ratio, age, comorbidities, or medications.Conclusions: These findings all suggest a relationship between hydration status and body composition but also set the bases for future studies that relate hydration status and anxiety status. These results can be used to improve the hydration status and body composition of military personnel. | Alejandra Carretero-Krug Nataliaúbeda Carlos Velasco Juan Medina-Font Trinidad Trujillo Laguna Gregorio Varela-Moreiras Ana Montero | 2022 | Military Medical Research2022,9,2: | 0 |
| 9 | Characterization of an exocellular β-glucosidase from Debaryomyces pseudopolymorphus显示文摘 | María Arévalo Villena Juan F. úbeda Iranzo Sarath B. Gundllapalli Ricardo R. Cordero Otero Ana I. Briones Pérez | 2005 | Enzyme and Microbial Technology2005,,2: | 1 |
| 10 | Mielitis transversa en síndrome de Sj?gren y lupus eritematoso sistémico: presentación de 3 casos显示文摘 | Raúl Menor Almagro María del Mar Ruiz Tudela Juan Girón úbeda Mario H. Cardiel Rios José Javier Pérez Venegas Carmen García Guijo | 2014 | Reumatologia Clinica2014,,: | 1 |