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| 1 | 野葛根中葛根素的提取及HPTLC测定显示文摘采用有机溶剂萃取结合酸水解的方法 ,直接从野葛根中分离葛根素 ,并用 HPTL C进行纯度分析。产率为1.15 % ,含量为 97.6 %。操作简便 ,产品纯度和产率高 。 | 张尊听 杨伯伦 刘谦光 刘鹏 | 2001 | 中国医药工业杂志2001，32，7： | 12 |
| 2 | β-榄香烯对人膀胱癌BIU-87细胞磷脂膜功能及Bcl-2表达的影响显示文摘目的探讨β-榄香烯对人膀胱癌BIU-87细胞磷脂膜功能及Bcl-2表达的影响。方法[32P]Pi同位素参入,提取细胞磷脂进行HPTLC分析细胞磷脂(PC及PE)代谢,考马斯亮蓝法测定细胞膜蛋白水平,分析细胞脂膜流动性及流式细胞仪测定Bcl-2表达的变化。结果随着β-榄香烯浓度的增加,[32P]Pi参入细胞PC、PE量明显降低,膜蛋白水平及Bcl-2表达明显降低,其最大抑制率分别为:41.1%、43.3%、43%、50%,呈明显的量效关系(P<0.05);β-榄香烯明显降低BIU-87细胞膜脂流动性。结论β-榄香烯可以明显抑制BIU-87细胞磷脂代谢、Bcl-2表达及脂膜流动性,提示磷脂(PC及PE)及Bcl-2与膀胱癌BIU-87细胞凋亡有密切关系。 | 李传刚 李墨林 周琴 舒晓宏 刘用楫 韩国柱 | 2007 | 中草药2007，38，6： | 11 |
| 3 | 太白山野葛根中异黄酮和葛根素含量随季节的变化显示文摘为了考察太白山野葛根中异黄酮及葛根素含量随季节的变化 ,采集一年 12个月份的野葛根 ,采用紫外分光光度法测定其中异黄酮含量 ;以高效薄层色谱扫描法测定野葛根中游离葛根素及总葛根素含量。结果表明 :三月份异黄酮含量最高 ,为 18 5 3%;四月份游离葛根素及总葛根素含量最高 ,分别为 7 4 5 %和 8 93%。对不同季节太白山野葛根中异黄酮、游离葛根素及总葛根素进行定量分析 ,为这一野生资源的合理采收和开发利用提供了实验依据。 | 张尊听 杨伯伦 刘谦光 张莉 | 2001 | 天然产物研究与开发2001，13，6： | 10 |
| 4 | 甘草黄酮的提取及影响活性因素研究显示文摘研究了我国3种主要甘草品种的总黄酮提取方法,采用HPTLC法定性检测和分光光度法测定含量,并应用对DPPH清除率研究影响其活性的几个因素.结果表明:在总黄酮的含量方面,光果甘草>乌拉尔甘草>胀果甘草;甘草品种、应用微波处理和甘草放置时间等因素对活性都有着较大影响,而提取温度对活性几乎不产生影响. | 罗锋 王咏梅 舒尊哲 杨玲 | 2006 | 四川师范大学学报(自然科学版)2006，29，5： | 9 |
| 5 | 3种五味子药材HPTLC鉴别与HPLC含量测定显示文摘目的建立五味子HPTLC定性鉴别和HPLC含量测定方法。方法以五味子甲素、五味子乙素、五味子醇甲、五味子酯甲为对照品,采用HPTLC法带状点样于GF254板上,以石油醚(30~60℃)-甲酸乙酯-甲酸(15∶5∶1)为展开剂,上行展开8 cm,置于紫外灯254nm下观察,比较不同来源的五味子活性成分的差异。以五味子醇甲为对照品,采用HPLC法,ZORBAX SB-C18(4.6 mm×250 mm,5μm),流动相:甲醇-水(65∶35);流速:1.0 ml.min-1;柱温:室温;检测波长:254 nm,比较不同来源的五味子醇甲的含量。结果 HPTLC法能够准确快速鉴别不同来源的五味子;3种药材中的五味子醇甲含量有差异。结论该方法简便、准确、重现性好,可从定性、定量两方面评价五味子药材质量。 | 李立顺 时维静 王贵阳 | 2011 | 时珍国医国药2011，22，7： | 8 |
| 6 | 6种有机磷农药分离及水中残留高效薄层析测定方法研究显示文摘[目的]比较有机磷农药的分离方法,用高效薄层色谱测定农药在水中残留。[方法]采用高效薄层析技术AMD和双槽展缸上行展开方式分离水中辛硫磷、毒死蜱、双硫磷、甲基异柳磷、喹硫磷和三唑磷6种有机磷农药,并对自来水中4种有机磷残留的测定方法进行研究。[结果]两种展开方式均能很好分离6种农药。AMD展开Rf值分布范围较窄(相差0.34),双槽展缸上行展开Rf值分布范围较宽(相差0.76);AMD多步展开检测灵敏度较双槽展缸上行展开低。采用双槽展缸上行展开薄层色谱法检测水中有机磷农药残留,添加回收率在86.05%-109.80%之间,添加回收率、变异系数均满足农药残留分析的要求。[结论]该研究中,双槽展缸上行展开表现出灵敏度较高、操作简便、分析速度快的特点。 | 张蓉 花日茂 汤锋 曹德菊 王馨蕾 | 2009 | 安徽农业科学2009，37，14： | 7 |
| 7 | Antimicrobial activity of Cannabis sativa,Thuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus显示文摘Objective: This study examined the antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava against methicillin-resistant Staphylococcus aureus(MRSA) and used a standardized purification protocol to determine the presence and abundance of bioactive compounds in the leaf extracts.Methods: In vitro antimicrobial activities of the ethanolic extracts of C. sativa, T. orientalis and P. guajava were tested against MRSA. The presence of bioactive molecules in these three leaves was evaluated using biochemical assays and high-performance thin-layer chromatography(HPTLC).Results: Resistance to methicillin, penicillin, oxacillin and cefoxitin was observed in each of the clinical and nonclinical MRSA isolates. However, they were still vulnerable to vancomycin. Used individually,the 50% extract of each plant leaf inhibited MRSA growth. A profound synergism was observed when C. sativa was used in combination with T. orientalis(1:1) and when P. guajava was used in combination with T. orientalis(1:1). This was shown by larger zones of inhibition. This synergism was probably due to the combined inhibitory effect of phenolics present in the leaf extracts(i.e., quercetin and gallic acid)and catechin, as detected by HPTLC.Conclusion: The leaf extracts of C. sativa, T. orientalis and P. guajava had potential for the control of both hospital-and community-acquired MRSA. Moreover, the inhibitory effect was enhanced when extracts were used in combination. | Shohini Chakraborty Nashra Afaq Neelam Singh Sukanta Majumdar | 2018 | Journal of Integrative Medicine2018，16，5： | 7 |
| 8 | Chromatographic finger print analysis of steroids in Aerva lanata L by HPTLC technique显示文摘Objective:To determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata(A.lanata) L.Methods:Preliminary phytochemicul screening was done by the method as Harborne described.HPTLC studies were canied out as Harborne and Wagner et al described.The Ethyl acetate-ethanol-water(8:2:1.2) was employed as mobile phase for glycosides.Results:The desired aim was achieved using Chloroform-acetone(8:2) as the mobile phase.The methanolic extract of stem,leaves,root,flower and seeds of A.lanata showed the presence of 30 different types of steroids with 30 different Rf values from 0.04 to 0.97. Maximum number(11) of steroids has been observed in leaves followed by root(10).Conclusions: HPTLC profile of steroids has been chosen here to reveal the diversity existing in A.lanata.Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies. | Yamunadevi Mariswamy Wesely Edward Gnaraj Johnson M | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，6： | 6 |
| 9 | 4种不同基原穿山甲炮制品的鉴定显示文摘目的:建立穿山甲饮片鉴定方法。方法:运用砂烫法对穿山甲进行炮制,首先从形状、大小、色泽、质地特征等方面对不同基原炮山甲饮片性状进行观察与描述;其次,采用二次展开法对高效薄层板进行扫描,利用Camag TLC Scanner4 System软件进行分析,同时采用TOF MS-IDA-MS/MS对炮山甲进行成分分析,使用Markerview等分析软件,通过PCA分析,寻找各来源穿山甲炮制品之间的差异。结果:炮制品中,穿山甲、马来穿山甲的性状及化学成分较为类似,可归为一类;树穿山甲、印度穿山甲炮制品的性状及化学成分差异较大,各自归为一类,同时各来源穿山甲经炮制后均产生丝-酪环二肽,可以作为鉴定穿山甲炮制品的共性指标成分。结论:性状、HPTLC结合TOF MS-IDA-MS/MS,可有效鉴别不同基原的穿山甲炮制品,为穿山甲的鉴定提供科学依据。 | 刘逊 张华峰 刘雪梅 朱缨 崔小兵 | 2017 | 中药材2017，40，3： | 6 |
| 10 | 银杏聚戊烯醇同系物的提取及其HPTLC法的初步分析显示文摘目的 :确定银杏聚戊烯醇各同系物的链长。方法 :从银杏叶的石油醚提取物中分离获得了纯度较高的聚戊烯醇同系物的混合物。进而利用反相高效薄层色谱 ,以丙酮为展开剂 ,碘蒸气显色后呈显五个斑点。结果 :经与标准品对照 ,可确定为聚戊烯醇 16~聚戊烯醇 2 0五个单体。结论 :用HPTLC能快速、简便地确定各同系物链长 ,较HPLC方法费用低廉。 | 杨小明 谢吉民 陈钧 | 2000 | 镇江医学院学报2000，10，4： | 5 |
| 11 | HPTLC-DPPH法定性定量分析保健品中芦丁显示文摘将1,1-二苯基-2-三硝基苯肼(DPPH)还原变色反应与高效薄层色谱分离相结合,建立了一种HPTLC-DPPH联用测定保健品中芦丁的方法,并考察了DPPH显色反应时间、流动相配比、光密度扫描参数对色谱分离、扫描效果的影响,同时测试了该方法的回收率和精密度。结果显示,该方法的标准添加回收率为82%~97%,精密度RSD<10%,且可消除保健品中复杂基质的干扰,适用于保健品中芦丁的快速测定。 | 王天楠 张煌 陈益胜 徐学明 黄彩虹 | 2019 | 食品与机械2019，35，1： | 4 |
| 12 | 啤酒中的生物胺和多胺显示文摘从毒理和技术的角度来看,食物和酒精饮料中存在的生物胺和多胺是很重要的,这些物质过量摄入能引起健康问题.在酿造过程中,胺的类型取决于原料,酿造方法,以及酿造或储存过程中发生的微生物污染.在欧洲,巴西,加拿大和古巴,一些研究人员已经对各种啤酒中生物胺和多胺的水平进行了研究.这篇文章回顾了以前的研究工作,概括了各研究小组的成果.胺的分析方法包括HPLC,HPTLC和酶的免疫分析. | 彭小勇 林琛 | 2004 | 啤酒科技2004，，2： | 4 |
| 13 | Preparation of κ-carra-Oligosaccharides with Microwave Assisted Acid Hydrolysis Method显示文摘A rapid method of microwave assisted acid hydrolysis was established to prepare κ-carra-oligosaccharides. The optimal hydrolysis condition was determined by an orthogonal test. The degree of polymerization(DP) of oligosaccharides was detected by high performance thin layer chromatography(HPTLC) and polyacrylamide gel electrophoresis(PAGE). Considering the results of HPTLC and PAGE, the optimum condition of microwave assisted acid hydrolysis was determined. The concentration of κ-carrageenan was 5 mg m L-1; the reaction solution was adjusted to p H 3 with diluted hydrochloric acid; the solution was hydrolyzed under microwave irradiation at 100 for 15℃ min. Oligosaccharides were separated by a Superdex 30 column(2.6 cm × 90 cm) using AKTA Purifier UPC100 and detected with an online refractive index detector. Each fraction was characterized by electrospray ionization mass spectrometry(ESI-MS). The data showed that odd-numbered κ-carra-oligosaccharides with DP ranging from 3 to 21 could be obtained with this method, and the structures of the oligosaccharides were consistent with those obtained by traditional mild acid hydrolysis. The new method was more convenient, efficient and environment-friendly than traditional mild acid hydrolysis. Our results provided a useful reference for the preparation of oligosaccharides from other polysaccharides. | LI Guangsheng ZHAO Xia LV Youjing LI Miaomiao YU Guangli | 2015 | Journal of Ocean University of China2015，14，2： | 3 |
| 14 | 华支睾吸虫来源极性差异化合物的高效薄层色谱免疫分析显示文摘目的探索华支睾吸虫ESPs、虫卵和虫体水溶性组分中生物大分子极性和抗原性的差异并寻找可行的分离方法。方法用HPTLC法分离华支睾吸虫ESPs、虫卵和虫体的水溶性组分,同时结合免疫染色法研究极性差异组分的抗原性。结果用有机溶剂组合可将ESPs、虫卵和虫体水溶性组分中极性强弱差异的化合物分离开。氯仿︰甲醇︰水(11︰9︰2)溶剂组合对弱极性化合物各组分有较好的分离效果。与虫卵相比较,ESPs和虫体的弱极性组分较复杂。不能被有机溶剂展开的强极性组分的抗原性较强,未检测出弱极性组分的抗原性反应。通过有机溶剂提取的弱极性组分可完全在水中复溶并被有机溶剂重新展开。结论华支睾吸虫来源的水溶性组分中的弱极性化合物成分能通过有机溶剂组合进行有效分离。这为分离华支睾吸虫来源的脂类等弱极性生物大分子以及研究其病原相关分子模式的性质奠定了基础。 | 杨庆利 申继清 蒋智华 陈颖丹 周晓农 | 2014 | 中国病原生物学杂志2014，9，9： | 3 |
| 15 | Densitometric HPTLC analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements,teas and commercial creams显示文摘Objective:To develop and validate a simple,accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol inZingiber officinaleextract and gingercontaining dietary supplements,teas and commercial creams.Methods:The analysis was performed on 10×20 cm aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F254(E-Merck,Germany)with n-hexane:ethyl acetate 60:40(v/v)as mobile phase.Camag TLC Scanner III was used for the UV densitometric scanning at 569.Results:This system was found to give a compact spot of 8-gingerol at retention factor(Rf) value of(0.39依0.04)and linearity was found in the ranges 50-500 ng/spot(r2=0.9987).Limit of detection(12.76 ng/spot),limit of quantification(26.32 ng/spot),accuracy(less than 2%)and recovery(ranging from 98.22-99.20)were found satisfactory.Conclusions:The HPTLC method developed for quantification of 8-gingerol was found to be simple,accurate,reproducible,sensitive and is applicable to the analysis of 8-gingerol in Zingiber officinaleextract and ginger-containing dietary supplements,teas and commercial creams. | Prawez Alam | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，8： | 3 |
| 16 | Quantification of total phenol,flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits显示文摘Objective:To carry out the physicochemical and phytochemical standardization with high performance thin layer chromatography fingerprinting of Piper nigrum L.(P.nigrum)fruits in order to ascertain the standard pharmacognostical parameters of this king of spices.Methods:Many standardization parameters like extractive values,total ash value,water soluble ash value and acid insoluble ash,moisture content,loss on drying and pH values of P.nigrum L.fruits were analyzed.The method of Harborne was adopted for the preliminary phytochemicals screening.Analysis of total phenolic and flavonoid contents,pesticides residues,aflatoxin and heavy metals were also performed.CAMAG-high performance thin layer chromatography system was used for fingerprinting of methanolic extract of P.nigrum L.fruits.Results:The results of phytochemicals testing indicated the presence of carbohydrates,phenolic compounds,flavonoids,alkaloids,proteins,saponins,lipids,sterols and tannins in various solvent extracts.Total phenolic and flavonoid contents in methanolic extract were found to be 1.728 1 mg/g and 1.087 ug/g,respectively.Heavy metals concentrations were found to be within standard limits.Aflatoxins and pesticides residues were absent.Conclusions:The outcome of this study might prove beneficial in herbal industries for identification,purification and standardization of P.nigrum L.fruits. | Aftab Ahmad Asif Husain Mohd Mujeeb Shah Alam Khan Hani Abdullah Anber Alhadrami Anil Bhandari | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，2： | 3 |
| 17 | 高效薄层色谱法检测黄藤素中掺杂的黄连素显示文摘采用高效薄层预制板,用双向展开薄层色谱法分离检测黄藤素中掺杂的人工合成黄连素,在波长λ=365nm下荧光定位,用薄层扫描法测定其含量。此法还可测定黄藤素中巴马汀与药根碱的含量,方法简便、快速、准确、重现性好。 | 郑萍 谢笑天 李国宝 范亚刚 | 1997 | 中草药1997，28，9： | 3 |
| 18 | 红豆杉属植物中紫杉醇类化合物的色谱分析显示文摘本文介绍了几种 HPLC 和 HPTLC 法分离。 | 靳朝东 徐莉 | 1997 | 国外医药(植物药分册)1997，12，1： | 3 |
| 19 | 建立稳定性指示性的分析方法在药物分析领域的研究进展显示文摘目的梳理稳定性指示性的分析方法在药物分析领域的研究进展。方法综合文献报道,从法规技术要求、方法建立、方法应用等方面进行论述。结果多项国际技术法规对稳定性指示性分析方法提供了要求和指导,稳定性指示性的HPTLC、HPLC、CE等方法广泛应用于药物质量控制和稳定性研究。结论建立稳定性指示性的分析方法在药品研发、生产中发挥着重要的作用,快速、高专属性、高选择性、高样品处理能力是这一方法的发展趋势。 | 郝福 胡向青 | 2011 | 中国医药指南2011，9，29： | 3 |
| 20 | Morpho-anatomical and physicochemical studies of Fumaria indica(Hausskn.)Pugsley显示文摘Objective:To study morpho-anatomical characters and physicochemical analysis of Fumaria indica(F.indica)(Hausskn.)Pugsley,(Fumariaceae),an important medicinal plant used extensively for treating a variety of ailments in various system of indigenous medicine.Methods:Evaluation of the different parts of the plant was carried out to determine the morphoanatomical,physicochemical,phytochemical and HPTLC fingerprinting profile of F.indica and other WHO recommended methods were performed for standardization.Results:Morphoanatomical studies showed compound and pinnatifid leaf,4 to 6 cm in length,linear and oblong in shape and anomocytic arrangement of stomata,thin walled parenchymatous cells,scattered,sclerenchymatous,capped vascular bundles and radiating medullary rays.Physicochemical studies showed foreign matter 0.2%,loss on drying 6.8%,total ash 16.77%,alcohol and water soluble extractives 8.92%and 20.26%,respectively,sugar 17.75%,starch 22.97%and tannins 2.37%.Phytochemical evaluation revealed the presence of carbohydrate,alkaloids,flavonoids,saponins,tannins and sterol.Thin layer chromatography was carried out with different solvents and the best solvent system was chloroform and methanol in 80:20 ratio and revealed 12 spots with different R_f value under UV light 366λ.Conclusions:The results of the study can serve as a valuable source of information and provide suitable standards for identification of this plant material for future investigations and applications. | Prakash Chandra Gupta Ch V Rao | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，10： | 2 |
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