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| 1 | AutoCAD二次开发方法的研究显示文摘由于CAD技术在功能与角色等方面的需求不断变化,AutoCAD的二次开发技术对于广大设计人员以及开发者来说就显得尤为重要。本文对AutoCAD的二次开发技术和方法进行了系统的总结,并详细分析了各种方法的应用特点以及优缺点。以便广大AutoCAD二次开发人员能够更好地掌握与应用AutoCAD完备的二次开发功能。 | 王永辉 胡青泥 李红彩 | 2007 | 计算机系统应用2007，16，3： | 61 |
| 2 | 肺结核患者治疗依从性研究进展显示文摘肺结核患者治疗依从性即患者严格按医嘱坚持服药的程度,决定着疾病的疗效和转归。本文就依从性的概念、判断方法、影响因素、直接面视下服药(DOT)的局限性等方面作一综述。 | 林定文 | 2014 | 热带医学杂志2014，14，7： | 13 |
| 3 | Mutations in pre-core and basic core promoter regions of hepatitis B virus in chronic hepatitis B patients显示文摘AIM: To investigate the frequency of mutations in precore(pre-C) and basic core promoter(BCP) regions of hepatitis B virus(HBV) from Shanxi Province, and the association between mutations and disease related indexes.METHODS: One hundred chronic hepatitis B patients treated at Shanxi Province Hospital of Traditional Chinese Medicine were included in this study. PCRreverse dot blot hybridization and mismatch amplification mutation assay(MAMA)-PCR were used to detect the mutations in the HBV pre-C and BCP regions. HBV DNA content and liver function were compared between patients with mutant HBV pre-C and BCP loci and those with wild-type loci. The consistency between PCR-reverse dot blot hybridization and MAMA-PCR for detecting mutations in the HBV pre-C and BCP regions was assessed.RESULTS: Of the 100 serum samples detected, 9.38% had single mutations in the pre-C region, 29.17% had single mutations in the BCP region, 41.67% had mutations in both BCP and pre-C regions, and 19.79% had wild-type loci. The rates of BCP and pre-C mutations were 65.7% and 34.3%, respectively, in hepatitis B e antigen(HBe Ag) positive patients, and 84.6% and 96.2%, respectively, in HBe Ag negative patients. The rate of pre-C mutations was significantly higher in HBe Ag negative patients than in HBe Ag positive patients(χ~2 = 26.62, P = 0.00), but there was no significant difference in the distribution of mutations in the BCP region between HBeAg positive and negative patients(χ~2 = 2.43, P = 0.12). The presence of mutations in the pre-C(Wilcoxon W = 1802.5, P = 0.00) and BCP regions(Wilcoxon W = 2906.5, P = 0.00) was more common in patients with low HBV DNA content. Both AST and GGT were significantly higher in patients with mutant pre-C and BCP loci than in those with wild-type loci(P < 0.05). PCR-reverse dot blot hybridization and MAMA-PCR for detection of mutations in the BCP and pre-C regions had good consistency, and the Kappa values obtained were 0.91 and 0.58, respectively.CONCLUSION: HBe Ag negative patients tend to have HBV pre-C mutations. However, these mutations do not cause increased DNA copies, but associate with damage of liver function. | Xiao-Ling Wang Jian-Ping Ren Xue-Qing Wang Xiao-Hong Wang Shao-Fang Yang Yi Xiong | 2016 | World Journal of Gastroenterology2016，22，11： | 12 |
| 4 | Dot-ELISA检测鸭瘟病毒的研究显示文摘用斑点酶联免疫吸附试验检测15头份鸭瘟强毒人工发病雏鸭的粪便,检出率达87%,三次重复检测,符合率达100%。该法简便、快速、准确、经济,易于在基层推广应用。 | 徐耀基 黄引贤 | 1992 | 中国畜禽传染病1992，14，2： | 11 |
| 5 | 分布对象技术述评显示文摘分析了几种主流的分布对象技术 ,并对它们进行了比较 ,同时指出了分布对象技术未来的发展方向。 | 赵东 周明天 | 2000 | 计算机应用2000，20，12： | 11 |
| 6 | PCR-Dotblot杂交法直接检测临床病原菌的报告显示文摘目的为了寻找临床病原菌系统,检测和鉴别的有力手段。方法用真细菌保守的16SrRNA基因为模板,以PCR-Dotblot杂交的方法检测临床病原菌。结果它将16SrRNA基因的广谱性和变异性并存之特点和PCR-Dotblot杂交的敏感性结合起来,对该法的建立进行了探讨,并初步用于临床感染的检测。结论为细菌通用检测法的建立提供了基础。 | 徐芸 杨瑞馥 郭兆彪 李继昌 | 1998 | 中华医院感染学杂志1998，8，1： | 11 |
| 7 | Profile, spectrum and significance of hepatitisB virus genotypes in chronic HBV-infected patients in Yunnan, China显示文摘BACKGROUND: There are significant variations in the geographical distribution of hepatitis B virus (HBV) genotypes throughout the world, and some genotypes are associated with different clinical outcomes. Eight genotypes of human HBV (designated A-H) have been reported. The present study was designed to examine the distribution of HBV genotypes among patients at various stages of chronic type B liver disease in Yunnan Province, China, and to explore its significance and the relationship of HBV genotype with gender and age, clinical spectrum of chronic HBV infection, and viral replicative activity. METHODS: Serum samples from 126 patients with chronic HBV infection from Yunnan Province, including 26 chronic asymptomatic HBV carriers (ASC), 61 patients with chronic hepatitis B (CHB) (21 mild, 30 moderate and 10 severe), 20 patients with chronic fulminant hepatic failure (CFHF), 12 patients with HBV-related liver cirrhosis (LC) and 7 patients with HBV-related hepatocellular carcinoma (HCC) were analyzed using reverse dot blot (RDB) methodology, which is based on the reverse hybridization principle for HBV genotyping. The relations of HBV genotype with gender and age,clinical patterns, and serological data of the patients were analyzed. RESULTS: In this series, genotypes A, B, C, and D were found. 38.1% patients (48/126) belonged to B, 54.8% (69/126) to C, 0.8% (1/126) to D, 1.6% (2/126) to a mixture of B and C, and 1.6% (2/126) to a mixture of A and C. 3.2% patients (4/126) had unknown genotypes. No other genotypes (E, F, G, and H) were found. Genotypes B and C were predominant. There was a statistically significant difference in the distributions of genotypes C and B (χ2=7.04, P=0.008), and C was the dominant genotype in all patient categories. The rate of genotype B in the mild CHB group was significantly higher than that in the moderate and severe groups (χ2=12.16, P=0.0001; χ2=11.98, P=0.001, respectively), the ASC group (χ2=5.46, P=0.02), the CFHF group (χ2=5.53, P=0.019), and the LC/HCC group (χ2=12.13, P=0.001). The rate of genotype C in the LC/HCC group and the severe CHB group were significantly higher than that in the mild group (χ2=9.95, P=0.002; χ2=8.78, P=0.003, respectively). HBV DNA positivity and HBeAg positivity were higher in genotype C than in genotype B (χ2=9.81, P=0.002; χ2=3.85, P=0.05, respectively). The prevalence of genotype C showed an increasing trend in lowest-, middle- and highest-level groups of HBV replication (25.0%, 70.0%, and 55.6%, respectively); in contrast, the prevalence of genotype B showed an opposite trend in the same order (62.5%, 30.0%, and 37.0%, respectively). The rate of genotype C in the highest-level group of HBV replication was higher than genotype B (χ2=7.45, P=0.006). The rate of genotype C in the over-30 age group was higher than that in the below-30 age group (χ2=3.7, P=0.05). There was no difference between the sexes (P>0.05). More severe liver damage was found in genotype C than in genotype B (P<0.05). CONCLUSIONS: The predominant HBV genotypes in chronic HBV-infected patients are B and C, and C is the most prevalent genotype in Yunnan Province, China. HBV genotype C is associated with the development of more severe liver disease and a higher level of HBV viral replication, and genotype B has a relatively good progress. | Hutcha Sriplung Virasakdi Chongsuvivatwong Alan Geater | 2008 | Hepatobiliary & Pancreatic Diseases International2008，7，3： | 9 |
| 8 | Polycyclic aromatic hydrocarbons in the graphene era显示文摘Polycyclic aromatic hydrocarbons(PAHs) have been the subject of interdisciplinary research in the fields of chemistry, physics,materials science, and biology. Notably, PAHs have drawn increasing attention since the discovery of graphene, which has been regarded as the 'wonder' material in the 21 st century. Different from semimetallic graphene, nanoscale graphenes, such as graphene nanoribbons and graphene quantum dots, exhibit finite band gaps owing to the quantum confinement, making them attractive semiconductors for next-generation electronic applications. Researches based on PAHs and graphenes have expanded rapidly over the past decade, thereby posing a challenge in conducting a comprehensive review. This study aims to interconnect the fields of PAHs and graphenes, which have mainly been discussed separately. In particular, by selecting representative examples, we explain how these two domains can stimulate each other. We hope that this integrated approach can offer new opportunities and further promote synergistic developments in these fields. | Xiao-Ye Wang Xuelin Yao Klaus Mullen | 2019 | Science China Chemistry2019，62，9： | 9 |
| 9 | Reverse Dot Blot Analysis: A Rapid Prenatal Diagnostic Approach for β-thalassemia Mutations in Chinese显示文摘The recently developed method of in vitro DNA amplification by PCR coupled with radioactive or nonradioactive ASO probe detection provides a simple approach to the prenatal diagnosis of β-thalassemia. However, the DNA diagnosis of β-thalassemia has remained a complicated problem,because β-thalassemia | 张基增 徐湘民 马维芳 彭朝晖 | 1994 | Chinese Science Bulletin1994，39，19： | 7 |
| 10 | Size and Magnetic Field Dependence of Strong Coupling Magnetopolaron in Quantum Dot显示文摘1IntroductionWiththeadvancesinthenanofabricationtechnology,itisposibletofabricatequantumdots[1-3]inwhichtheelectronenergyspec... | Zhou Haiyang(Collage of Sciences, Shanghai University) Gu Shiwei(Shanghai Jiaotong University) | 1999 | Advances in Manufacturing1999，，2： | 7 |
| 11 | Preliminary study of a dot immunogold filtration assay for rapid detection of anti-HCV IgG显示文摘INTRODUCTIONHepatitisCisaworldwideepidemicdiseasestemmingfromthehepatitisCvirus(HCV).HCVisnotonlythepathogenicfactorofhepati... | XIAO Le Yi 1, YAN Xiao Jun 1, MI Ming Ren 2, HAN Feng Chan 1 and HOU Yu 1 | 1999 | World Journal of Gastroenterology1999，5，4： | 7 |
| 12 | 树舌多糖GF对小鼠HepA癌细胞PTEN蛋白表达的影响显示文摘目的探明树舌多糖(GAPS)GF对小鼠HepA癌细胞PTEN蛋白表达的影响。方法昆明种小鼠随机分肿瘤组和树舌多糖组。接种后各组肌肉注射给药,分别给予生理盐水和树舌多糖GF。连续10d,于末次给药24h,处死小鼠,剥离肿瘤组织。Dot blot技术检测各组样品PTEN蛋白的表达量。结果肿瘤组检测结果为阴性,树舌多糖组检测结果为阳性。结论树舌多糖GF抗肿瘤的作用靶点之一可能为抑癌基因PTEN。 | 宋高臣 于水澜 于英君 | 2008 | 中国老年学杂志2008，28，11： | 6 |
| 13 | Semiconductor quantum computation显示文摘Semiconductors, a significant type of material in the information era, are becoming more and more powerful in the field of quantum information. In recent decades, semiconductor quantum computation was investigated thoroughly across the world and developed with a dramatically fast speed. The research varied from initialization, control and readout of qubits, to the architecture of fault-tolerant quantum computing.Here, we first introduce the basic ideas for quantum computing, and then discuss the developments of single-and two-qubit gate control in semiconductors. Up to now, the qubit initialization, control and readout can be realized with relatively high fidelity and a programmable two-qubit quantum processor has even been demonstrated. However, to further improve the qubit quality and scale it up, there are still some challenges to resolve such as the improvement of the readout method, material development and scalable designs. We discuss these issues and introduce the forefronts of progress. Finally, considering the positive trend of the research on semiconductor quantum devices and recent theoretical work on the applications of quantum computation, we anticipate that semiconductor quantum computation may develop fast and will have a huge impact on our lives in the near future. | Xin Zhang Hai-Ou Li Gang Cao Ming Xiao Guang-Can Guo Guo-Ping Guo | 2019 | National Science Review2019，6，1： | 6 |
| 14 | 转基因猪研究进展显示文摘本文综述了湖北省农科院生物技术研究所有关转基因猪研究的主要进展。先后将POMT PGH ,PSV4 0 PGH ,POMT PGH MicrosatelliteDNA ,PMHR ,hDAF ,pBHSA ,pSHSA等7种不同结构的外源基因,注入4 0 2 7枚猪的受精卵原核,获得原代表达的转基因猪83头,转基因的效率在0 9%~3 4 %之间,平均2 2 %。通过几个主要技术环节的改进,提高了转基因的效率;建立了外源基因整合的定性检测(PCR ,Dotblot和Southernblot)和定位检测染色体原位杂交技术;进行了相应外源基因的蛋白质表达检测; | 魏庆信 郑新民 赵浩斌 华文君 李莉 田永祥 魏雁 | 2005 | 中国比较医学杂志2005，15，2： | 6 |
| 15 | 美国政府对气瓶的安全监管简介显示文摘美国政府对气瓶的安全监督管理是世界范围内政府所能采取的最严格的管理模式,其管理机构是美国运输部,运输部的英文缩写是DOT。本文将从DOT的工作法律、法规依据、机构设置、人员配备和工作方式等诸方面进行介绍,以期给读者一个较完整的概念,希望本文能对我国加入WTO后,研究政府职能如何适应市场经济有所借鉴。 | 胡军 吴燕 | 2002 | 中国锅炉压力容器安全2002，18，2： | 6 |
| 16 | Dot immunogold filtration assay for rapid detection of anti-HAV IgM in Chinese显示文摘INTRODUCTION The hepatitis A virus specific immunoglobulin M(IgM)antibody is a specific serological marker forearly diagnosis of hepatitis A..At present,themethods used at home or abroad for detecting anti-HAV IgM are RIA,ELISA and SPHAI.The dotimmunogold combination assay that has beendeveloped since 1989 is a new technique with theproperty of simple and rapid immunologicaldetection,by using the red colloidal gold particles tolabel the antibodies as indicator,and the | Han FC Hou Y Yan XJ Xiao LY Guo YH | 2000 | World Journal of Gastroenterology2000，6，3： | 5 |
| 17 | PCR-Dotblot在慢性前列腺炎诊断中应用的研究显示文摘目的 建立基于16SrRNA基因的PCR-Dot blot细菌学检测方法,用于慢性前列腺炎患者EPS的细菌学检查,以指导临床诊断和治疗。方法 建立PCR-Dot blot方法,并对实验组58例标本和对照组30例标本进行检测。结果 经过优化的PCR方法检测细菌的敏感性达到了103/mL。实验组标本PCR检测阳性率46.6%(27/58),其中包括培养法阳性的4例标本占实验组总数的7.0%(4/58)。对照组标本PCR检测阳性率为10%(3/30)。经过杂交检测实验组标本中20例为G-菌,7例为G+细菌,对照组3例PCR阳性标本都为G+细菌。结论 基于16SrRNA基因的PCR-Dot blot方法具有快速、简便、稳定、不受干扰等优点,可用于检测慢性前列腺炎患者的EPS。 | 赵岩 鹿占鹏 沈明 周立权 | 2005 | 中国男科学杂志2005，19，1： | 5 |
| 18 | 谷氨酸棒杆菌合成新型生物絮凝剂分批发酵过程的溶氧控制模式显示文摘在3L发酵罐上系统研究溶氧水平对谷氨酸棒杆菌菌体生长及新型生物絮凝剂REA 11合成的影响,提出生物絮凝剂REA 11合成的分阶段供氧控制策略:发酵过程0~16h维持体积传氧系数kLa为100h-1,16h后降低kLa为40h-1至发酵结束,整个发酵过程通气量保持在1L·L-1·min-1.采用该分阶段供氧控制策略,生物絮凝剂最终产量达到900mg·L-1,发酵周期缩短到30h,比恒定kLa为40h-1条件下的REA 11产量(549mg·L-1)提高了64%,产率提高了45%,生产强度也比kLa恒定为40h-1,100h-1和200h-1的分批发酵过程分别提高了81 2%,120%和420%,实现了高细胞生长速率和高产物产率的统一. | 何宁 李寅 陈坚 李清彪 | 2004 | 环境科学学报2004，24，3： | 5 |
| 19 | 苦参素、膦甲酸钠和阿德福韦酯联合治疗乙型肝炎的体外研究显示文摘目的研究苦参素(OMTR)、膦甲酸钠(PFA)和阿德福韦酯(ADV)体外联合用药抑制HBV DNA的作用。方法采用Dot Blot分析法,将不同浓度的OMTR、ADV、PFA以及三种药物间任意矩阵组合,分别作用于2215细胞,一周后,采用同位素杂交测定HBV DNA的表达量,凝胶扫描分析软件对其进行半定量分析,比较联合用药与单独用药的抗病毒效果。结果结果表明,与单种药物作用相比,不同浓度OMTR、PFA和ADV合理联合作用,显著抑制了HBV DNA复制,对HBV DNA的抑制效果显示较强的相加作用。结论本实验通过比较三种药物联合与单独用药体外抗病毒效果研究,证实了不同作用机制的药物联合作用,相比单种药物,可以显著增强抗病毒效果,为临床联合用药治疗乙型肝炎提供了重要的理论基础。 | 王宇萍 韩燕星 蒋建东 | 2016 | 中华临床医师杂志(电子版)2016，10，15： | 4 |
| 20 | MoS2 Nanosheets Sensitized with Quantum Dots for Room-Temperature Gas Sensors显示文摘The Internet of things for environment monitoring requires high performance with low power-consumption gas sensors which could be easily integrated into large-scale sensor network.While semiconductor gas sensors have many advantages such as excellent sensitivity and low cost,their application is limited by their high operating temperature.Two-dimensional(2D)layered materials,typically molybdenum disulfide(MoS2)nanosheets,are emerging as promising gas-sensing materials candidates owing to their abundant edge sites and high in-plane carrier mobility.This work aims to overcome the sluggish and weak response as well as incomplete recovery of MoS2 gas sensors at room temperature by sensitizing MoS2 nanosheets with PbS quantum dots(QDs).The huge amount of surface dangling bonds of QDs enables them to be ideal receptors for gas molecules.The sensitized MoS2 gas sensor exhibited fast and recoverable response when operated at room temperature,and the limit of NO2 detection was estimated to be 94 ppb.The strategy of sensitizing 2D nanosheets with sensitive QD receptors may enhance receptor and transducer functions as well as the utility factor that determine the sensor performance,offering a powerful new degree of freedom to the surface and interface engineering of semiconductor gas sensors. | Jingyao Liu Zhixiang Hu Yuzhu Zhang HuaYao Li Naibo Gao Zhilai Tian Licheng Zhou Baohui Zhang Jiang Tang Jianbing Zhang Fei Yi Huan Liu | 2020 | Nano-Micro Letters2020，12，5： | 3 |
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