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| 1 | 日本血吸虫雌虫抗原模拟表位的筛选及免疫保护性显示文摘目的 筛选日本血吸虫雌虫抗原的模拟表位 ,并探讨其抗日本血吸虫的免疫保护效果。 方法 用日本血吸虫雌虫免疫兔血清IgG作配体对噬菌体随机 12肽库进行 3轮亲和筛选 ,随机挑取 18个噬菌体克隆用Dot ELISA检测其特异性 ,并对其中的 4个阳性克隆进行测序。分别在 0、2、4周用混合噬菌体克隆免疫小鼠 3次 ,第 6周每鼠经腹部感染 40条日本血吸虫尾蚴 ,42d后剖杀冲虫 ,计数虫数和每克肝卵数。 结果 经 3轮筛选 ,特异性噬菌体富集了 2 0 0多倍 ,随机挑取的 18个克隆经Dot ELISA鉴定有 17个能与雌虫抗原免疫兔血清呈阳性反应。DNA自动测序的 4个序列与GenBank的已知序列均无同源性。与对照组相比 ,混合噬菌体克隆免疫小鼠的减虫率为 2 6.5 7% ,减卵率为 65 .3 4%。 结论 采用噬菌体随机肽库技术可获得模拟日本血吸虫雌虫特异性抗原表位的短肽分子 ,这些短肽分子能诱导一定程度的保护性免疫。 | 王林纤 蔡春 易新元 曾宪芳 唐连飞 罗永慧 | 2004 | 中国寄生虫病防治杂志2004，17，2： | 4 |
| 2 | 东方田鼠及其天然抗日本血吸虫感染的特性显示文摘 | 李小红 阎玉涛 刘述先 | 2005 | 中国血吸虫病防治杂志2005，17，2： | 2 |
| 3 | Identification and Characterization of Peptides Mimicking the Epitopes of Metalloprotease of Schistosoma Japonicum显示文摘In an attempt to isolate and characterize peptides mimicking epitopes of metalloprotease and explore their immunological protection against Schistosoma japonicum (S. japonicum), polyclonal anti-metalloprotease sera was prepared to screen a 12-mer random peptide library to isolate phages binding specially to antisera IgG. Then,phage ELISA, animal immunization, DNA sequencing, Western blotting and enzymatic activity neutralizing analysis were used to characterize the selected phage clones. All of ten randomly picked clones were shown to be positive. Five peptides of different amino acid sequences deduced from DNA sequences were obtained and two of them (peptides 2 and 3) could induce significant reduction (31.0% and 31.8%, respectively) in worm burden and high reduction (52.6% and 54.9%, respectively) in liver eggs per gram (LEPG), while, unexpectedly, others (peptides 1, 4 and 5) could not elicit enough protection against infection of S. japonicum. Peptides 2 and 3 could be recognized by S. japonicum infected mouse sera (IMS) and could elicit neutralizing Abs. The results show that peptides 2 and 3 are antigenic and immunogenic. They are true mimics of epitopes of metalloprotease and useful as novel vaccine candidates against S. japonicum. | Lianfei Tang Yuxiao Chen Linqian Wang Shunke Zhang Xianfang Zeng Xinyuan Yi | 2005 | Cellular & Molecular Immunology2005，2，3： | 2 |
| 4 | 噬菌体展示技术及其在抗血吸虫疫苗研究中的应用显示文摘噬菌体展示技术是新近发展起来的将外源肽或蛋白与特定噬菌体衣壳蛋白融合,并展示于噬菌体表面的一项新技术。展示系统主要包括丝状噬菌体展示系统、λ噬菌体展示系统、T4噬菌体展示系统及T7噬菌体展示系统等。该技术在抗血吸虫疫苗研究上的应用主要集中在模拟血吸虫抗原位点和直接作为疫苗载体方面,而在分析血吸虫保护性抗原位点、发现新的功能蛋白或抗原基因方面的应用研究还有待加强。本文就噬菌体展示技术及其在抗血吸虫疫苗研制中的应用进行了简要概述,为抗血吸虫疫苗的研究提供。 | 孙毅 刘金明 | 2008 | 中国兽医寄生虫病2008，16，1： | 1 |
| 5 | 日本血吸虫候选疫苗免疫保护力研究进展显示文摘血吸虫病是危害严重的寄生虫病之一,其疫苗的研究是血吸虫病防治的一项工作重点也是一大难题。本文综述了日本血吸虫疫苗侯选分子筛选、模拟表位疫苗和多价疫苗的研究现状,并对血吸虫疫苗的深入研究进行了分析。 | 黄复深 伍小松 陈尔曼 | 2007 | 中国兽医寄生虫病2007，15，4： | 0 |
| 6 | Phage displaying peptides mimic schistosoma antigenic epitopes selected by rat natural antibodies and protective immunity induced by their immunization in mice显示文摘AIM: To obtain the short peptides mimic antigenic epitopes selected by rat natural antibodies to schistosomes, and to explore their immunoprotection against schistosomiasis in mice.METHODS: Adults worm antigens (AWA) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked transferred immunoblotting methods with normal SD rat sera (NRS). The killing effects on schistosomula with fresh and heat-inactivated sera from SD rats were observed. Then the purified IgG from sera of SD rats was used to biopan a phage random peptide library and 20 randomly selected positive clones were detected by ELISA and 2 of them were sequenced.Sixty female mice were immunized thrice with positive phage clones (0, 2nd, 4th wk). Each mouse was challenged with 40 cercariae, and all mice were killed 42 d after challenge. The worms and the liver eggs were counted. RESULTS: NRS could specifically react to the molecules of 75 000, 47 000, 34 500 and 23 000 of AWA. Sera from SD rats showed that the mortality rate of schistosomula was 76.2%, and when the sera were heat-inactivated in vitro, the mortality rate was decreased to 41.0% after being cultured for 48 h. The specific phages bound to IgG were enriched about 300-folds after three rounds of biopanning. Twenty clones were detected by ELISA, 19 of them bound to the specific IgG of rat sera. Immunization with these epitopes was carried out in mice. Compared with the control groups, the mixture of two mimic peptides could induce 34.9% (P = 0.000) worm reduction and 67.6% (P = 0.000) total liver egg reduction in mice. Two different mimic peptides could respectively induce 31.0% (P = 0.001), 14.5% (P = 0.074) worm reduction and 61.2% (P = 0.000), 35.7% (P = 0.000) total liver egg reduction. The specific antibody could be induced by immunization of the mimic peptides, and the antibody titer in immunized mice reached more than 1:6 400 as detected by ELISA.CONCLUSION: Specific peptides mimic antigenic molecules can be obtained by biopanning the phage random peptide library and a partially protective immunity against schistosome infection can be stimulated by these phage epitopes in mice. | MinWang Xin-YuanYi Xian-PingLi Dong-MingZhou McReynoldsLarry Xian-FangZeng | 2005 | World Journal of Gastroenterology2005，11，19： | 0 |
| 7 | 噬菌体展示技术及其在血吸虫研究中的应用显示文摘血吸虫是严重危害人类健康的寄生虫之一,近几年噬菌体展示技术为血吸虫病防治研究提供了新的思路和方法,在血吸虫病的诊断、治疗、预防中起到一定的作用,现综述如下。 | 雷黎 沈际佳 | 2005 | 国外医学(寄生虫病分册)2005，32，5： | 0 |
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