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| 1 | New insights into the carotenoid biosynthesis in Torreya grandis kernels显示文摘Torreya grandis is a characteristic rare economic tree species in subtropical mountainous areas. The nut has a variety of bioactive compounds, such as polyphenols and vitamins. However, the carotenoids compositions and its regulatory mechanisms underlying carotenoid biosynthesis in kernels remain unknown. Here, the major carotenoids in the kernel were analyzed. The result showed that lutein and β-carotene were the predominant carotenoids in the kernel, while low levels of a-carotenoid and zeaxanthin were detected. Lutein and β-carotene were decreased during the maturation of kernel. Expression analysis by RNA-seq and q RT-PCR indicated that the expressions of TgCYP97A3 and TgLCYB were also reduced during the maturation of kernel. The contents of lutein or β-carotene were obviously increased in tobacco transiently overexpressing TgCYP97A3 or TgLCYB. Moreover, DPPH radical scavenging activity and FRAP were also significantly enhanced. In addition, several MYB and WRKY transcription factors TgMYBS3, TgMYB48 and TgWRKY11 were identified to positively regulate the TgCYP97A3 expression, while TgMYB48, TgWRKY2 and TgWRKY11 could upregulate the TgLCYB expression. The illustration of carotenoids biosynthesis and its molecular mechanism in kernels not only provides a basis for understanding carotenoids biosynthesis in kernels, but also enables the use of molecular biotechnology to develop new health products rich in carotenoids based on T. grandis nuts. | Jingwei Yan Hao Zeng Weijie Chen Jiali Luo Congcong Kong Heqiang Lou Jiasheng Wu | 2023 | Horticultural Plant Journal2023,9,6: | 0 |
| 2 | 干旱胁迫下大蒜幼苗的代谢组学分析显示文摘利用高效液相色谱串联质谱广泛靶向代谢组学的方法,通过主成分分析、正交偏最小二乘判别分析、聚类分析、富集分析、相关性分析等方法,测定并比较干旱胁迫和对照大蒜幼苗叶片的代谢物质成分。结果共检测出23类743种代谢物,其中313种上调,430种下调,差异代谢物134种,占总体代谢物的18.03%。上、下调差异代谢物质数量最多的为黄酮、苯丙素、黄酮醇、黄酮类、核苷酸及衍生物、氨基酸及衍生物、糖类、有机酸及衍生物等8类物质,主要富集在黄酮和黄烷酮的生物合成途径中,干旱胁迫后大蒜幼苗叶片黄酮差异代谢物数量较多且上调。对大蒜黄酮代谢途径进行分析,咖啡酸和麦黄酮O–丙二酰己糖苷的上调关联代谢物质最多,推测干旱胁迫下黄酮和苯丙素物质含量的增加会显著提高大蒜的抗旱性。 | 田洁 周倩怡 铁原毓 孙海宏 黄思杰 | 2024 | 园艺学报2024,51,1: | 0 |
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