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1Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal显示文摘A grand challenge facing society is climate change caused mainly by rising CO_(2) concentration in Earth’s atmosphere.Terrestrial plants are linchpins in global carbon cycling,with a unique capability of capturing CO_(2) via photosynthesis and translocating captured carbon to stems,roots,and soils for long-term storage.However,many researchers postulate that existing land plants cannot meet the ambitious requirement for CO_(2) removal to mitigate climate change in the future due to low photosynthetic efficiency,limited carbon allocation for long-term storage,and low suitability for the bioeconomy.To address these limitations,there is an urgent need for genetic improvement of existing plants or construction of novel plant systems through biosystems design(or biodesign).Here,we summarize validated biological parts(e.g.,protein-encoding genes and noncoding RNAs)for biological engineering of carbon dioxide removal(CDR)traits in terrestrial plants to accelerate land-based decarbonization in bioenergy plantations and agricultural settings and promote a vibrant bioeconomy.Specifically,we first summarize the framework of plant-based CDR(e.g.,CO_(2) capture,translocation,storage,and conversion to value-added products).Then,we highlight some representative biological parts,with experimental evidence,in this framework.Finally,we discuss challenges and strategies for the identification and curation of biological parts for CDR engineering in plants.Xiaohan Yang Degao Liu Haiwei Lu David J.Weston Jin-Gui Chen Wellington Muchero Stanton Martin Yang Liu Md Mahmudul Hassan Guoliang Yuan Udaya C.Kalluri Timothy J.Tschaplinski Julie C.Mitchell Stan D.Wullschleger Gerald A.Tuskan 2021BioDesign Research2021,,1:0
2Plant Biosystems Design Research Roadmap 1.0显示文摘Human life intimately depends on plants for food,biomaterials,health,energy,and a sustainable environment.Various plants have been genetically improved mostly through breeding,along with limited modification via genetic engineering,yet they are still not able to meet the ever-increasing needs,in terms of both quantity and quality,resulting from the rapid increase in world population and expected standards of living.A step change that may address these challenges would be to expand the potential of plants using biosystems design approaches.This represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological systems.Plant biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant genomes.From this perspective,we present a comprehensive roadmap of plant biosystems design covering theories,principles,and technical methods,along with potential applications in basic and applied plant biology research.We highlight current challenges,future opportunities,and research priorities,along with a framework for international collaboration,towards rapid advancement of this emerging interdisciplinary area of research.Finally,we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception,trust,and acceptance.Xiaohan Yang June I.Medford Kasey Markel Patrick M.Shih Henrique C.De Paoli Cong T.Trinh Alistair J.McCormick Raphael Ployet Steven G.Hussey Alexander A.Myburg Poul Erik Jensen Md Mahmudul Hassan Jin Zhang Wellington Muchero Udaya C.Kalluri Hengfu Yin Renying Zhuo Paul E.Abraham Jin-Gui Chen David J.Weston Yinong Yang Degao Liu Yi Li Jessy Labbe Bing Yang Jun Hyung Lee Robert W.Cottingham Stanton Martin Mengzhu Lu Timothy J.Tschaplinski Guoliang Yuan Haiwei Lu Priya Ranjan Julie C.Mitchell Stan D.Wullschleger Gerald A.Tuskan 2020BioDesign Research2020,,1:0
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