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| 1 | 新型DNA碱基编辑器的研究进展显示文摘DNA碱基编辑技术是由CRISPR/Cas系统发展而来,能对基因组碱基进行精准编辑。目前已开发的DNA碱基编辑器包括介导C·G至T·A转换的胞嘧啶单碱基编辑器、介导A·T至G·C转换的腺嘌呤单碱基编辑器、介导C·G至G·C颠换的糖基化酶单碱基编辑器、介导C·G至T·A和A·T至G·C同时转换的双碱基编辑器、介导任意碱基之间转换的引导编辑器以及线粒体DNA编辑器。本文系统总结了上述6种DNA编辑器的原理、优化历程及最新研究进展,着重介绍了应用到植物研究中的碱基编辑器工具及其在作物遗传改良中的应用,并对碱基编辑技术今后的发展进行了展望。 | 张雅玲 王锌和 李构思 曾栋昌 祝钦泷 陈乐天 刘耀光 | 2022 | 华南农业大学学报2022，43，6： | 4 |
| 2 | Present and future prospects for wheat improvement through genome editing and advanced technologies显示文摘Wheat(Triticum aestivum,2n=6x=42,AABBDD)is one of the most important staple food crops in the world.Despite the fact that wheat production has significantly increased over the past decades,future wheat production will face unprecedented challenges from global climate change,increasing world population,and water shortages in arid and semi-arid lands.Furthermore,excessive applications of diverse fertilizers and pesticides are exacerbating environmental pollution and ecological deterioration.To ensure global food and ecosystem security,it is essential to enhance the resilience of wheat production while minimizing environmental pollution through the use of cutting-edge technologies.However,the hexaploid genome and gene redundancy complicate advances in genetic research and precision gene modifications for wheat improvement,thus impeding the breeding of elite wheat cultivars.In this review,we first introduce state-of-the-art genome-editing technologies in crop plants,especially wheat,for both functional genomics and genetic improvement.We then outline applications of other technologies,such as GWAS,high-throughput genotyping and phenotyping,speed breeding,and synthetic biology,in wheat.Finally,we discuss existing challenges in wheat genome editing and future prospects for precision gene modifications using advanced genome-editing technologies.We conclude that the combination of genome editing and other molecular breeding strategies will greatly facilitate genetic improvement ofwheat for sustainable global production. | Shaoya Li Chen Zhang Jingying Li Lei Yan Ning Wang Lanqin Xia | 2021 | Plant Communications2021，2，4： | 4 |
| 3 | Crop genome editing: A way to breeding by design显示文摘Increasing population and consumption in our planet is placing unprecedented challenges on agriculture for meeting food security and sustainability needs[1].Meanwhile,the adaptation of modern agricultural techniques[2]is central to minimize extensive losses due to abiotic stresses[3]under global climate change. | Chuanxiao Xie Yunbi Xu Jianmin Wan | 2020 | The Crop Journal2020，8，3： | 4 |
| 4 | The power and versatility of genome editing tools in crop improvement显示文摘Increasing world population,global climate change,decreased farmland,environmental pollution and ecological deterioration represent unprecedent challenges for crop production to ensure global food security(Hickey et al.,2019;Li et al.,2021 a).It is estimated that by the year 2050,50% more food is needed to feed the increasing population(Bailey-Serres et al.,2019).Thus,it is urgent to boost crop production by using cutting-edge technologies. | Lanqin Xia Kejian Wang Jian-Kang Zhu | 2021 | Journal of Integrative Plant Biology2021，63，9： | 0 |
| 5 | 基因编辑技术及其在农作物中的应用进展显示文摘全球气候变化、人口增长、耕地减少和极端天气频发等为粮食安全和农业可持续发展带来诸多挑战。以CRISPR/Cas为代表的基因组编辑技术能够快速定向创制农作物新种质,提高育种效率,为保障粮食安全和生态安全提供有力的技术和材料支撑。综述了CRISPR/Cas系统介导的基因敲除、单碱基编辑、精准替换和引导编辑技术的研发及其在农作物遗传改良中的应用进展,并对我国农作物基因编辑研究进行了展望和建议,以期为今后农作物基因编辑研究的发展和应用提供参考。 | 闫磊 张金山 朱健康 夏兰琴 | 2022 | 中国农业科技导报2022，24，12： | 0 |
| 6 | 大蒜遗传转化体系建立与CRISPR-Cas9敲除蒜氨酸酶基因研究显示文摘为了为大蒜转基因研究和蒜氨酸酶基因研究提供技术支撑和理论依据,本文首先建立了愈伤组织再生培养体系,在基因型、生长发育时期、植物激素配比和外植体来源方面对比MS、B5、N6和SH基础培养基,明确大蒜愈伤诱导和培养的最适基础培养基为MS和SH,其中以苍山大蒜根尖为外植体、培养基为MS+1 mg/L 2,4-D+0.1 mg/L 6-BA是大蒜愈伤组织诱导的最佳组合;分化阶段为MS+3 mg/L 6-BA;生根培养和鳞茎诱导阶段则使用不添加任何激素的培养基。然后,通过对影响农杆菌介导遗传转化因素的筛选,得到最佳转化条件:农杆菌菌株选用LBA4404、侵染液组成MS/LB+100μM As+1%葡萄糖+1%蔗糖为宜,侵染时间10 min,共培养3 d,草铵膦筛选浓度为250 mg/L,在此条件下该方法的遗传转化率为1.68%。利用CRISPR-Cas9敲除技术,成功实现了对鳞茎蒜氨酸酶关键基因的敲除,突变体中目的基因mRNA的表达量和蒜氨酸酶活均明显下降。 | 牛忠露 杨彩霞 李宁阳 李祥 | 2023 | 山东农业大学学报(自然科学版)2023，54，3： | 0 |
| 7 | Plant Biosystems Design Research Roadmap 1.0显示文摘Human life intimately depends on plants for food,biomaterials,health,energy,and a sustainable environment.Various plants have been genetically improved mostly through breeding,along with limited modification via genetic engineering,yet they are still not able to meet the ever-increasing needs,in terms of both quantity and quality,resulting from the rapid increase in world population and expected standards of living.A step change that may address these challenges would be to expand the potential of plants using biosystems design approaches.This represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological systems.Plant biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant genomes.From this perspective,we present a comprehensive roadmap of plant biosystems design covering theories,principles,and technical methods,along with potential applications in basic and applied plant biology research.We highlight current challenges,future opportunities,and research priorities,along with a framework for international collaboration,towards rapid advancement of this emerging interdisciplinary area of research.Finally,we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception,trust,and acceptance. | Xiaohan Yang June I.Medford Kasey Markel Patrick M.Shih Henrique C.De Paoli Cong T.Trinh Alistair J.McCormick Raphael Ployet Steven G.Hussey Alexander A.Myburg Poul Erik Jensen Md Mahmudul Hassan Jin Zhang Wellington Muchero Udaya C.Kalluri Hengfu Yin Renying Zhuo Paul E.Abraham Jin-Gui Chen David J.Weston Yinong Yang Degao Liu Yi Li Jessy Labbe Bing Yang Jun Hyung Lee Robert W.Cottingham Stanton Martin Mengzhu Lu Timothy J.Tschaplinski Guoliang Yuan Haiwei Lu Priya Ranjan Julie C.Mitchell Stan D.Wullschleger Gerald A.Tuskan | 2020 | BioDesign Research2020，，1： | 0 |
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