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| 1 | Comparative evaluation of immune response after laparoscopical and open total mesorectal excisions with anal sphincter preservation in patients with rectal cancer显示文摘AIM: The study of immune response of open versus laparoscopical total mesorectal excision with anal sphincter preservation in patients with rectal cancer has not been reported yet. The dissected retroperitoneal area that contacts directly with carbon dioxide is extensive in laparoscopic total mesorectal excision with anal sphincter preservation surgery. Tt is important to clarify whether the immune response of laparoscopic total mesorectal excision with anal sphincter preservation (LTME with ASP) in patients with rectal cancer is suppressed more severely than that of open surgery (OTME with ASP). This study was designed to compare the immune functions after laparoscopic and open total mesorectal excision with anal sphincter preservation for rectal cancer.METHODS: This study involved 45 patients undergoing laparoscopic (n=20) and open (n=25) total mesorectal excisions with anal sphincter preservation for rectal cancer.Serum interleukin-2 (IL-2), interleukin-6 (IL-6), tumor necrosis factor α (TNFα) were assayed preoperatively and on days 1 and 5 postoperatively. CD3+ and CD56+ T lymphocyte count, CD3- and CD56+ natural killer cell (NK)count and immunoglobulin (IgG/IgM/IgA) were assayed preoperatively and on day 5 postoperatively. The numbers of CD3+ and CD56+ T lymphocytes and CD3- and CD56+ NK cells were counted using flow cytometry. An enzyme-linked immunosorbent assay (ELISA) was used for IL-2, TL-6 and TNFα determination. And IgG, IgM, and IgA were assayed using immunonephelometry.RESULTS: The demographic data of the two groups had no difference. The preoperative levels of CD3+ and CD56+ T lymphocyte count, CD3- and CD56+ NK count, serum IgG,IgM, IgA, IL-2, IL-6 and TNFα also had no significant difference in the two groups (P>0.05). The CD3+ and CD56+ T lymphocyte counts had no obvious changes after surgery in laparoscopic (d=-0.79±3.83 %) and open (d=0.42±2.09 %)groups. The CD3- and CD56+ NK counts were decreased postoperatively in both laparoscopic (d=-7.23±11.33 %) and open (d=-9.21±13.93 %) groups. The differences of the determined values of serum IgG, IgM and IgA on the fifth day after operation subtracted those before operation were -2.56±2.14 g/L, -252.35±392.94 mg/L, -506.15±912.24 mg/L in laparoscopic group, and -1.81±2.10 g/L, -282.72±356.75mg/L, -252.20±396.28 mg/L in open group, respectively. The levels of IL-2 were decreased after operation in both groups.However, the levels of IL-6 were decreased after laparoscopic surgery (d1=-23.14±263.97 ng/L and d5=-40.08±272.03 ng/L),and increased after open surgery (d1=27.38±129.14 ng/L and d5=21.67±234.31 ng/L). The TNFα levels were not elevated after surgery in both groups. There were no significant differences in the numbers of CD3+ and CD56+ T lymphocytes and CD3- and CD56+ NK cells, the levels of IgG, IgM, IgA,IL-2, IL-6 and TNFα between the two groups (P>0.05).CONCLUSION: There are no differences in immune responses between the patients having laparoscopic total mesorectal excision with anal sphincter preservation and those undergone open surgery for rectal cancer. | Jian-KunHu Zong-GuangZhou Zhi-XinChen Lan-LanWang Yong-YangYu JinLiu BoZhang LiLi YeShu Jia-PingChen | 2003 | World Journal of Gastroenterology2003，9，12： | 23 |
| 2 | Expression of tumor related genes NGX6,NAG-7,BRD7 in gastric and colorectal cancer显示文摘AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 genes in human gastric and colorectal cancer tissues, and their corresponding normal tissues, and to investigate whether these genes play a role in the pathogenesis of gastric and colorectal cancers.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), dot hybridization and Northern blot analysis were used to compare the expression levels of NGX6, NAG-7 and BRD7 genes in 34 gastric cancer tissues and 34 colorectal cancer tissues with their corresponding normal tissues of the same patients, respectively.RESULTS: Among the 34 colorectal cancer specimens and the 34 gastric cancer specimens, the expression of NGX6 in 25 colorectal cancer tissues was absent or very weak (73.5 %) by RT-PCR analysis. The down-regulation rate of NGX6 in colorectal cancer tissues was significantly higher than that in corresponding normal tissues (26.5 %,9/34)(P<0.005). Moreover, the down-regulation of NGX6 was significantly correlated with lymph node and/or distance metastases. Patients with lymph node and/or distance metastasis had much higher down-regulation rate of NGX6 than patients without metastases (93.8 % vs 55.6 %, P<0.05).However no correlation was found between the expression of NGX6 and pathologic type of colorectal cancer in this study, and also the expression of NGX6 did not display any difference between gastric cancer and corresponding normal tissues (58.8 % vs 70.6 %, P>0.25). Dot hybridization and Northern blot analysis confirmed the results of RT-PCR.Furthermore, NAG-7 and BRD7 mRNA was not up- or downregulated in gastric and colorectal cancers compared with their corresponding normal tissues in our study.CONCLUSION: The down-regulation of NGX6 may be closely associated with tumorigenesis and metastasis of colorectal carcinoma. However, it may not contribute to the development and progression of gastric carcinoma. In addition, the expression levels of NAG-7, and BRD7 did not alter in gastric and colorectal cancers. This seems to suggest that NAG-7and BRD7 genes may not play a role in gastric and colorectal carcinogenesis. | Xiao-MeiZhang Shou-RongSheng Xiao-YanWang Jie-RuWang JiangLi | 2003 | World Journal of Gastroenterology2003，9，8： | 10 |
| 3 | MicroRNAs in pancreatic ductal adenocarcinoma显示文摘Ductal adenocarcinoma of the pancreas is a lethal cancer for which the only chance of long-term survival belongs to the patient with localized disease in whom a potentially curative resection can be done. Therefore, biomarkers for early detection and new therapeutic strategies are urgently needed. miRNAs are a recently discovered class of small endogenous non-coding RNAs of about 22 nucleotides that have gained attention for their role in downregulation of mRNA expression at the post-transcriptional level. miRNAs regulate proteins involved in critical cellular processes such as differentiation, proliferation, and apoptosis. Evidence suggests that deregulated miRNA expression is involved in carcinogenesis at many sites, including the pancreas. Aberrant expression of miRNAs may upregulate the expression of oncogenes or downregulate the expression of tumor suppressor genes, as well as play a role in other mechanisms of carcinogenesis. The purpose of this review is to summarize our knowledge of deregulated miRNA expression in pancreatic cancer and discuss the implication for potential translation of this knowledge into clinical practice. | Jong Y Park James Helm Domenico Coppola Donghwa Kim Mokenge Malafa Seung Joon Kim | 2011 | World Journal of Gastroenterology2011，17，7： | 7 |
| 4 | 散发性结直肠癌1号染色体等位基因杂合缺失精细定位研究显示文摘目的抑癌基因的杂合缺失(LOH)被认为是结直肠癌形成的通路之一。本实验通过对1号染色体1p36.33~36.31、1q31.1~32.1区域进行杂合缺失精细定位分析,以发现更精确的高频杂合缺失区域。方法在1p36.33~36.31、1q31.1~32.1区域分别选择7个、6个荧光标记微卫星引物与83例结直肠癌的肿瘤和正常组织进行聚合酶链反应(PCR)反应。产物在电泳后进行LOH分析。LOH结果与临床病理参数之间的关系比较采用x^2检验。结果1p36.33~36.31区域平均杂合缺失率是31.47%,以D1S243位点最高,为47.22%(34/72),最低是D1S1347,为7.35% (5/68)。存在两个高频杂合缺失区域:D1S243位点(1p36.33)以及D1S468-D1S2660区域(1p36.32~36.31)。1q31.1~32.1区域平均杂合缺失率是22.98%,以D1S2622位点最高,为36.73%(18/49),最低是D1S412,为16.42%(11/67)。更精确的缺失范围定位在D1S413和D1S2622之间(1q31.3~32.1),大约2cm的遗传距离范围内。1p36.33~36.31、1q31.1~32.1区域各位点的杂合缺失率与性别、年龄、肿瘤大小、生长方式以及Dukes分期无显著相关。提示该区域上的杂合缺失现象普遍存在于各种类型的散发性结直肠癌中。结论1号染色体上存在3个高频杂合缺失区域,D1S243位点(1cm)、D1S468和D1S2660位点之间(3cm)以及D1S413和D1S2622之间(2cm),提示在这些区域存在与结直肠癌相关的抑癌基因。 | 周崇治 裘国强 樊军卫 李大鹏 黄力 郑海涛 贺林 彭志海 | 2007 | 中华实验外科杂志2007，24，5： | 6 |
| 5 | Loss of heterozygosity on hromosome 1 in sporadic colorectal carcinoma显示文摘AIM: Loss of heterozygosity (LOH) on tumor suppressor genes is believed to play a key role in carcinogenesis of colorectal cancer. When it occurs at a tumor suppressor gene locus with abnormal allele, neoplastic transformation happens. In this study, we analyzed the LOH at 21 loci on chromosome 1 in sporadic colorectal cancer to identify additional loci involved in colorectal tumorigenesis.METHODS: Twenty-one polymorphic micro-satellite DNA markers were analyzed with PCR both in 83 cases of colorectal cancer and in normal tissues. PCR products were eletrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis. X^2 test was used to compare LOH frequency with clinicopathological data. P<0.05 was considered as statistically significant.RESULTS: The average LOH frequency of chromosome 1,short arm and long arm was 19.83%, 18.00% and 21.66%,respectively. The 2 highest LOH loci with a frequency of 36.54% and 32.50% were identified on DIS468 (1p36.33-p36.31) and DIS413 (1q31.3), respectively. On DIS2726 locus, LOH frequency of rectal cancer was 28.57% (6/21),which was higher than that of colon cancer (0.00%, 0/33) (P=0.002), suggesting that the mechanism of carcinogenisis was different in both groups.CONCLUSION: Putative tumor suppressor genes on chromosome 1 may relate to sporadic colorectal carcinomas.Tumor-suppressor-genes might locate on 1p36.33-36.31and/or 1q31.3. | Chong-ZhiZhou Guo-QiangQiu FangZhang LinHe Zhi-HaiPeng | 2004 | World Journal of Gastroenterology2004，10，10： | 6 |
| 6 | Angiography for diagnosis and treatment of colorectal cancer显示文摘AIM: To evaluate the role of preoperative angiography inthe diagnosis and treatment of colorectal cancer.METHODS: The authors performed selective arterialcannulation by Seldinger's method in 47 patients to locatethe primary cancer and to diagnose metastasis to the liver.Each patient was then given intra-arterial regionalchemotherapy, and received 5-fluorouracil (5-Fu, 1 000 mg),mitomycin C (MMC, 20 mg), and cisplatinum (CDDP, 80 mg).RESULTS: The location and shape of each tumor wereobserved, including metastatic tumors in the liver, in 42 ofthe 47 (89.4 %) patients. The site of the primary tumor wasdifficult to identify in 5 cases because the patients had arecurrence of cancer. Arterial chemotherapy was performedsuccessfully in all patients. The authors recorded no partialor significant morbidity resulted from angiography. The onlyincident was bleeding from the artery puncture site in onepatient, which was successfully stopped by generalmedication.CONCLUSION: Preoperative selective arterial angiographycan help the diagnosis and locate primary tumors and todetect liver metastasis. At the same time, regional arterialchemotherapy can be an important form of preoperativetherapy. | JinGu Zhao-LaiMa YingLi MingLi Guang-WeiXu | 2003 | World Journal of Gastroenterology2003，9，2： | 6 |
| 7 | 多重荧光PCR-毛细管电泳进行微卫星不稳定性分析中的常见问题显示文摘 | 潘菲 宋坤 盛弘强 来茂德 | 2008 | 临床与实验病理学杂志2008，24，5： | 5 |
| 8 | Refinement of heterozygosity loss on chromosome 5p15 in sporadic colorectal cancer显示文摘AIM: To refine the loss of heterozygosity on chromosome 5p15 and to identify the new tumor suppressor gene (s) in colorectal tumorigenesis.METHODS: Sixteen polymorphic microsatellite markers were analyzed on chromosome 5 and another 6 markers were applied on chromosome 5p15 in 83 cases of colorectal and normal DNA by PCR. PCR products were electrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis.RESULTS: We observed 2 distinct regions of frequent allelic deletions on Chromosome 5, at D5S416 on 5p15 and D5S428-D5S410 on 5q. Another 6 polymorphric microsatellite markers were applied to 5p15 and the minimal region of frequent loss of heterozygosity was established on 5p15spanning the D5S416 locus.CONCLUSION: Through our detailed deletion mapping studies, we have found a critical and precise location of 5p deletions, 5p15.2-5p15.3, which must contain one or more unknown tumor suppressor gene (s) of colorectal cancer. | Shi-Feng Xu Zhi-Hai Peng Da-Peng Li Guo-Qiang Qiu Fang Zhang, Department of General Surgery, Shanghai First People’s Hospital, Shanghai 200080, China | 2003 | World Journal of Gastroenterology2003，9，8： | 5 |
| 9 | 人源性大肠癌抗原基因的SEREX筛选显示文摘目的:寻找人源性大肠癌相关抗原基因.方法:构建3个以入Tripl Ex2噬菌体作载体的大肠癌抗原cDNA表达文库,用自体或异体大肠癌患者血清应用SEREX方法进行免疫筛选.用平板法扩增阳性克隆噬菌体,提取纯化DNA,用SfiI酶切和PCR鉴定插入片段的大小.结果:构建成3个大肠癌抗原cDNA噬菌体表达文库,滴度分别为2.39×106nfu/L,2.07×106nfu/L和1.86×106nfu/L,插入片段长度为0.5-4kb,平均分别为1.4kb,1.6kb和1.3kb.筛选发现4个阳性克隆,插入cDNA片段大小分别为2.4 kb,1.8 kb,2.3 kb和2.2 kb.结论:用SEREX方法筛选大肠癌抗原cDNA表达文库,可获得有价值的大肠癌的重组抗原基因克隆,将有利于大肠癌的早期诊断和重组疫苗的研究. | 刘宇虎 张振书 钟东 武金宝 但汉雷 赖卓胜 王亚东 张亚历 肖冰 | 2003 | 世界华人消化杂志2003，11，9： | 4 |
| 10 | 散发性结直肠癌1p36.33-36.31区域等位基因杂合缺失精细定位显示文摘目的抑癌基因的杂合缺失(LOH)被认为是结直肠癌形成的通路之一,本实验通过对染色体1p36.33-36.31区域的杂合缺失精细定位分析,发现高频杂合缺失区域并探讨其意义。方法7个荧光标记的微卫星引物(1p36.33-36.31)与83例结直肠癌的肿瘤和正常组织进行聚合酶链反应(PCR)。产物在ABI Prism 377自动荧光测序仪进行电泳,以GeneSean3.1和Genotyper 2.1软件进行扫描以及杂合缺失分析。杂合缺失结果与临床病理参数之间的关系比较采用X2检验。结果1p36.33-36.31区域平均杂合缺失率是31.5%,以D1S243位点最高,47.2%,最低是D1S1347,7.4%。存在两个高频杂合缺失区域:D1S243位点(1p36.33)以及D1S468和D1S2660位点之间约3 cM的区域(1p36.32-36.31)。1p36.33-36.31区域各位点的杂合缺失率与性别、年龄、肿瘤大小生长方式以及分级无显著相关。结论1p36.33.36.31区域发现了两个高频LOH区域:1p36.33和1p36.32-36.31,可能存在与结直肠癌发生相关的抑癌基因。 | 周崇治 郑海涛 裘国强 张放 贺林 彭志海 | 2006 | 中华医学杂志2006，86，26： | 3 |
| 11 | Detailed deletion mapping of loss of heterozygosity on 22q13 in sporadic colorectal cancer显示文摘AIM: Both development and progression of malignancies occur as a multistep process, requiring the activation of oncogenes and the inactivation of several tumor suppressor genes. The loss of heterozygosity (LOH) of tumor suppressor genes is believed to play a key role in carcinogenesis of colorectal cancer (CRC).In this study, we analyzed the LOH of seven loci on chromosome 22q13 in an effort to identify candidate tumor suppressor genes involved in colorectal carcinogenesis.METHODS: Matched tumor and normal tissue DNA were analyzed by PCR using fluorescence-labeled polymorphic microsatellite markers in 83 CRC patients. PCR products were eletrophoresed and LOH was determined by calculating the peak height acquired through computer software. Comparisons between LOH frequency and clinicopathological features Were performed by χ2 test.P<0.05 was considered as statistical significance.RESULTS: The average LOH frequency of chromosome 22q13 was 28.38%. The highest LOH frequency was 64.71% on D22S1160 locus, and the lowest was 21.43%on D22S1141 locus. We detected two obvious minimal deletion regions: one between markers D22S1171 and D22S274, the other flanked by markers D22S1160 and D22S1149, each about 2.7 and 1.8 cm, respectively. None had lost in all informative loci. LOH frequency on D22S1171is 50% on distal colon, which was higher than that on proximal one (P = 0.020); on D22S114 locus, none LOH event occurred in patients with liver metastasis, whilst 46.94% occurred in patients without liver metastasis (P= 0.008); on D22S1160 locus, LOH frequency in lymph nodes metastasis patients was 83.33%, which was much higher than 43.75% without lymph nodes metastasis ones (P = 0.016). There was no statistical significance between clinicopathological features and other loci.CONCLUSION: This study provides evidence of two minimal deletion regions, which may harbor putative tumor suppressor genes related to progression and metastasis in sporadic colorectal carcinoma on chromosome 22q13. | Hai-TaoZheng Zhi-HaiPeng Chong-ZhiZhou Da-PengLi Zhao-WenWang Guo-QiangQiu LinHe | 2005 | World Journal of Gastroenterology2005，11，11： | 2 |
| 12 | 散发性结直肠癌染色体10q23~24区域杂合缺失分析显示文摘目的抑癌基因的杂合缺失(LOH)被认为是结直肠癌形成的通路之一,本实验拟通过对染色体10q23~24区的LOH分析,发现高频杂合缺失区域并筛查与结直肠癌相关的抑癌基因。方法7个荧光标记的微卫星引物(围绕D10S185位点)与83例结直肠癌的肿瘤和正常组织进行聚合酶链反应(PCR)反应。产物在ABIPrism377自动荧光测序仪进行电泳,以GeneScan3.1和Genotyper2.1软件进行扫描以及杂合缺失分析LOH。与临床病理因素之间的关系比较采用χ2检验。结果7个位点平均杂合缺失率为36.11%,以D10S583位点最高,达54.84%;最低是D10S205,21.3%。发现两个高频杂合缺失区域:一个在D10S583和D10S185之间,大约0.9cM(10q23.33)的距离;另一个在D10S1709和D10S1265位点之间,大约1.5cM(10q24.2~24.31)的距离。D10S1265位点的杂合缺失与Dukes分期显著相关,其余位点与临床病理因素均无显著相关。结论在散发性结直肠癌10q2324发现了两个高频LOH区域:10q23.33和10q24.2~24.31。除对磷脂酶同族蛋白(PTEN)基因外,10q上可能存在与散发性结直肠癌相关的其他抑癌基因。 | 郑海涛 彭志海 周崇治 王兆文 裘国强 张放 贺林 | 2005 | 中华医学杂志2005，85，30： | 2 |
| 13 | 同时性结直肠癌肝转移临床病理因素分析显示文摘目的 探讨结直肠癌发生肝转移与其临床病理因素的关系。方法 分析比较有肝转移 117例与DukesC或D期无肝转移 5 0例结直肠癌病人的临床特点、血清CEA水平、转移淋巴结、以及原发灶的病理类型和静脉侵犯。结果 以肝转移灶为首诊原因的有 2 1例 ,伴肝转移结直肠癌患者远距离淋巴结转移及镜下静脉侵犯发生率升高 ,与对照组比较差异显著 (P <0 .0 5 )。结论 要重视结直肠癌肝转移的早期诊断 。 | 杨明智 彭志海 王兆文 裘国强 | 2004 | 肿瘤2004，24，4： | 1 |
| 14 | 散发性结直肠癌22q13区域杂合缺失的精细定位分析显示文摘目的在染色体高频杂合缺失区22q13精细定位,以筛查可能与结直肠癌相关的肿瘤抑制基因。方法荧光标记的微卫星引物与83例结直肠癌的肿瘤和正常组织进行PCR反应。产物在ABIPrism377自动荧光测序仪进行电泳、扫描以及杂合缺失分析。其结果与临床病理因素进行相关性检验。结果8个位点平均杂合缺失率为35.6%。发现两个高频缺失区域:一个在D22S1171和D22S274之间,约2.7厘摩(cM);另一个在D22S1160和D22S1149位点之间,约1.8cM。D22S1171位点与肿瘤发生部位显著相关(P=0.020);D22S114位点与肝转移显著相关(P=0.008);D22S1160位点与淋巴结转移显著相关(P=0.016);其余位点与临床病理因素无显著相关性(P>0.05)。筛选发现ARHGAP8基因和PPARA基因可能是肿瘤抑制基因。结论散发性结直肠癌22q13区域存在两个高频杂合缺失区,分别约2.7cM及1.8cM。ARHGAP8基因和PPARA基因可能是22q13区域与散发性结直肠癌相关的肿瘤抑制基因。 | 郑海涛 唐华美 彭志海 周崇治 贺林 | 2006 | 中华胃肠外科杂志2006，9，2： | 1 |
| 15 | 散发性结直肠癌1号染色体短臂遗传位点杂合缺失显示文摘目的:抑癌基因的杂合缺失(loss of heterozygosity,LOH)被认为是结直肠癌形成的关键步骤之一。本实验研究了结直肠癌1号染色体短臂杂合的缺失情况,并探讨其临床意义。方法:选取11个微卫星DNA标记与83例结直肠癌病例的肿瘤和正常组织进行PCR。PCR产物在ABIPrism377自动荧光测序仪上进行电泳,以GeneScan3.1和Genotyper2.1软件进行遗传位点扫描以及杂合缺失分析。结果:1号染色体短臂的平均杂合缺失率为18.00%,D1S468(1p36.33-36.31)位点的杂合缺失率最高,达36.54%。D1S2726位点的杂合缺失现象主要存在于直肠癌,缺失率为28.57%(6/21),而结肠癌的缺失率为0.00%(0/33),二者差异具统计学意义(P=0.002)。结论:在1号染色体短臂上可能存在与结直肠癌发生相关的抑癌基因,位于1p36.33-36.31这个区域。 | 裘国强 周崇治 张放 贺林 彭志海 | 2007 | 肿瘤2007，27，1： | 1 |
| 16 | Refined mapping of loss of heterozygosity on 1q31.1-32.1 in sporadic colorectal carcinoma显示文摘AIM: To explore precise deleted regions and screen the candidate tumor suppressor genes related to sporadic colorectal carcinoma. METHODS: Six markers on 1q31.1-32.1 were chosen. These polymorphic microsatellite markers in 83 colorectal cancer patients tumor and normal DNA were analyzed via PCR. PCR products were electrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for Loss of heterozygosity (LOH) scanning and analysis. Comparison between LOH frequency and clinicopathological factors was performed by χ2 test. RESULTS: 1q31.1-32.1 exhibited higher LOH frequency in colorectal carcinoma. The average LOH frequency of 1q31.1-32.1 was 23.0%, with the highest frequency of 36.7% (18/49) at D1S2622, and the lowest of 16.4% (11/67) at D1S412, respectively. A minimal region of frequent deletion was located within a 2 cM genomic segment at D1S413-D1S2622 (1q31.3-32.1). There was no significant association between LOH of each marker on 1q31.1-32.1 and the clinicopathological data (patient sex, age, tumor size, growth pattern or Dukes stage), which indicated that on 1q31.1-32.1, LOH was a common phenomenon in all kinds of sporadic colorectal carcinoma. CONCLUSION: Through our refined deletion mapping,the critical and precise deleted region was located within 2 cM chromosomal segment encompassing 2 loci (D1S413, D1S2622). No significant association was found between LOH and clinicopathologic features in 1q31.1-32.1. | Chong-Zhi Zhou Guo-Qiang Qiu Jun-wei Fan Xiao-Liang Wang Hua-Mei Tang Li Huang Yu-Hao Sun Zhi-Hai Peng | 2008 | World Journal of Gastroenterology2008，14，10： | 1 |
| 17 | Amplification of D22S283 as a favorable prognostic indicator in liver fluke related cholangiocarcinoma显示文摘瞄准:为了分析目标基因 NF2, TIMP3, ST13, TOB2, BIK,和 TP 和参考的 DNA 拷贝数字,在肝吸虫在 22q12-qter 上印射的微卫星标记 D22S283, D22S423,和 D22S274 联系了 cholangiocarcinoma (CCA ) 并且与临床的参数定义它的关联。方法:量的实时 PCR (qPCR ) 被用于决定在 65 肝吸虫的突变而产生之遗传的不平衡联系了 CCA 纸巾。在突变而产生之遗传的不平衡和 clinicopathological 参数之间的统计关联,即年龄,性别,肿瘤阶段,组织学的类型,血管侵略,神经侵略和淋巴的侵略借助于 chi2 测试被评估。考克斯回归分析被用于决定病人的幸存。结果: TP ( 22q13.33 )的扩大, TOB2 ( 22q13.2-13.31 ), D22S283 ( 22q12.3 ), TIMP3 ( 22q12.3 )和 NF2 ( 22q12.2 )在 35 被发现(53.8%), 28 (43.1%), 27 (41.5%), 24 (36.9%),并且 24 (36.9%),分别地。在 D22S423 (22q13.1-13.2 ) 和 BIK (22q13.31 ) 的损失在 26 被检测(40%) 并且 23 (35.4%) 分别地。重要关联在淋巴的侵略和 BIK 的突变而产生之遗传的损失之间被观察(P = 0.025 ) 并且 D22S283 (P = 0.041 ) 。 Univariate 并且多,变量考克斯回归分析作为好预后的一个独立预言者揭示了 D22S283 扩大( P = 0.006 ,死亡危险比率= 0.411 ,95% CI = 0.217-0.779 )并且是的血管侵略一个独立人士差的预示的因素( P = 0.042 ,死亡危险比率= 1.911 ,95% CI = 1.022-3.571 )在 CCA 病人。结论:这研究提供证据因为在肝吸虫的染色体 22q 上的基因扩大和删除的参与联系了 CCA。当在肝吸虫的有利预后的独立指示物联系了 CCA,这是 D22S283 扩大的第一份报告。 | Jongkonnee Thanasai Temduang Limpaiboon Patcharee Jearanaikoon Vajarabhongsa Bhudhisawasdi Narong Khuntikeo Banchob Sripa Masanao Miwa | 2006 | World Journal of Gastroenterology2006，12，27： | 1 |
| 18 | 中国染色体蛋白质组计划研究进展显示文摘人类染色体蛋白质组计划(Chromosome-Centric Human Proteome Project,C-HPP)是由人类蛋白质组组织(Human Proteome Organization,HUPO)于2010年9月在悉尼召开的第九届国际蛋白质组学大会上正式提出的大型国际合作计划。与生理-病理驱动的人类蛋白质组计划(Biology/Disease-driven HPP,B/D-HPP)相同,C-HPP是人类蛋白质组计划(Human Proteome Project,HPP)的主要工作,将由中国、美国、澳大利亚、瑞士、意大利等17个国家的25个研究团队共同合作,旨在10年内(2012.09—2022.09)完成人类24条染色体和线粒体上蛋白质编码基因产物本身及其修饰产物的检测、验证和确认,注销可能过度注释的编码基因,绘制基因图谱并实现人类基因组的重注释。国内军事医学科学院的贺福初院士和徐平教授、复旦大学的杨芃原教授、华大基因的刘斯奇教授和暨南大学的何庆瑜教授,以及台湾地区的陈玉如教授分别领衔承担了人类第1号、8号、20号和第4号染色体上蛋白质编码基因的研究任务。综述了我国承担的C-HPP工作的策略和进展,并提出展望。 | 彭雪辉 苏纳 李宗桃 徐平 | 2016 | 生命科学2016，28，9： | 1 |
| 19 | 厦门地区大肠癌人群某些遗传性易感因子和抗性因子的研究显示文摘目的:比较15个STR基因座基因频率在厦门地区大肠癌患者和正常人群中的分布,推测与大肠癌相关的基因.方法:应用PCR复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查了本地区大肠癌患者人群和无关人群的基因频率分布,并根据二者的该15个基因座等位基因频率分布的显著性差异,推测易感连锁和抗性连锁的等位基因.结果:厦门地区大肠癌患者的D5S818(0.5200 vs 0,219 5,X2=36.69,P<0.01;RR=3.8521, P<0.05)、vWA(0.0500 vs 0.2927,X2=53.99, P<0.01;RR=0.1272,P<0.05)和FAG(0.09 vs 0.2439,X2=37.58,P<0.01:RR=0.3066, P<0.05)基因座的等位基因的分布与该地区健康人群有显著性差异,(P<0.01).B组超微结构改变明显,而C组较B组超微结构有不同程度减轻.结论:D5S818-11附近可能存在大肠癌易感基因;vWA-15、FAG-23附近有可能存在与大肠癌相关的抗性基因. | 黄如欣 游攀 陈长荣 张忠英 倪宏英 刘广发 任建林 | 2006 | 世界华人消化杂志2006，14，2： | 1 |
| 20 | 散发性结直肠癌1号染色体等位基因杂合缺失显示文摘杂合缺失(LOH)是指来自父方或母方的一个等位基因的缺失.抑癌基因的LOH被认为是结直肠癌形成的关键步骤之一,一条等位基因已经异常的抑癌基因发生LOH可导致该基因失活,并进一步导致肿瘤形成.目前,通过微卫星DNA标记对散发性肿瘤进行LOH分析,找出其共同缺失的片段,这是对抑癌基因进行定位克隆的主要策略之一.本实验研究结直肠癌1号染色体LOH情况并探讨其意义。 | 周崇治 郑海涛 裘国强 张放 贺林 彭志海 | 2006 | 中华消化杂志2006，26，7： | 0 |
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