共被期刊论文引用了2次
您的检索式：您选中1篇文献正在查看引证文献汇总
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 盐藻钙调素基因的克隆及表达分析显示文摘为探究盐藻钙调素基因的结构与功能,利用RT-PCR和RACE技术克隆到盐藻钙调素基因(DsCaM,GenBank登录号:MN428415),并对其进行生物信息学分析,通过qRT-PCR方法检测盐藻钙调素在盐胁迫条件下的表达情况。试验结果显示,盐藻钙调素基因cDNA全长1061 bp,开放阅读框495 bp,编码164个氨基酸。盐藻钙调素为亲水蛋白,该蛋白主要分布在细胞质和液泡内,无跨膜区域,不存在信号肽,蛋白质的二级结构以α-螺旋(56.71%)和无规则卷曲(26.22%)为主,成功构建蛋白质三级结构。系统进化分析表明,盐藻钙调素基因与莱茵衣藻钙调素基因亲缘关系最近。qRT-PCR结果表明,在高盐胁迫下盐藻钙调素基因的表达量显著上调,胁迫6 h时表达量达到最高值,差异达到极显著水平(P<0.01)。该研究成果将为进一步分析盐藻钙调素基因的功能及盐藻应答盐胁迫信号的分子途径提供新信息。 | 王明芳 高相楠 徐微微 丛玉婷 柴晓杰 | 2021 | 水产科学2021，40，3： | 0 |
| 2 | Functional Analysis of Dunaliella salina Calmodulin Kinase Gene显示文摘[Objectives] This study was conducted to investigate the function of Dunaliella salina calmodulin kinase(CaM K) gene.[Methods] The sense and antisense gene fragments(223 bp) and spacer sequence(129 bp) of D.salina calmodulin kinase gene were cloned and inserted into the downstream part of the35 S promoter of the eukaryotic expression vector pM DCMGN-Cat.The siRNA expression system of CaM K gene was successfully constructed.The p CaM K-RNAi expression vector was transformed into D.salina cells by the LiA c/PEG-mediated method,giving transgenic D.salina.The expression of CaM K gene was then analyzed by real-time fluorescence quantitative PCR.[Results]The expression of CaM K gene in the transgenic D.salina was significantly reduced,by 70% compared with the control group,suggesting that the expression of CaM K gene was significantly inhibited.The examination of the growth status of D.salina showed that D.salina cell division and proliferation were also affected.It is proved that CaM K gene has a positive regulation effect on the division and proliferation of D.salina cells.[Conclusions] The study provides important information for further elucidating the function and action mechanism of D.salina calmodulin kinase gene. | Zhenyu XING Mingfang WANG Xiangnan GAO Weiwei XU Yuting CONG Xiaojie CHAI | 2020 | Agricultural Biotechnology2020，9，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员