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| 1 | 亚低温干预下神经干细胞的RhoA/ROCK通路显示文摘背景:亚低温能有效减轻继发性脑和脊髓损伤,对中枢神经损伤有确切的保护作用;同时亚低温改善脊髓损伤区域的微环境,有利于移植细胞的增殖、活化、生长。目的:探讨神经干细胞微环境变化与RhoA/ROCK信号通路的关系。方法:制备神经干细胞液压损伤模型,随机分为2组:常温组和亚低温组,常温组不进行任何干预,亚低温组于伤后1 h进行亚低温[(32.0±0.5)℃]干预4 h。RT-PCR和Western Blot检测神经干细胞RhoA、RHOCK、Nogo-A、NgR基因和蛋白的表达,免疫荧光观察RhoA/RHOCK阳性细胞数,激光共聚焦显微镜根据荧光强度值测定神经细胞内游离Ca2+浓度。结果与结论:(1)亚低温组神经干细胞RhoA、RHOCK、Nogo-A、NgR mR NA和蛋白的表达较常温组明显降低(P<0.05);(2)亚低温组神经干细胞内游离钙离子浓度低于常温组(P<0.05);(3)亚低温组RhoA/RHOCK阳性细胞数明显低于常温组(P<0.05);(4)结果表明,在亚低温条件下,对神经干细胞内Rho A/ROCK信号通路激活环节产生阻断作用,进而调控神经干细胞增殖、凋亡等。 | 李超 付红杰 张建军 王东 | 2017 | 中国组织工程研究2017，21，13： | 2 |
| 2 | ROCK inhibition enhances neurite outgrowth in neural stem cells by upregulating YAP expression in vitro显示文摘Spontaneous axonal regeneration of neurons does not occur after spinal cord injury because of inhibition by myelin and other inhibitory factors. Studies have demonstrated that blocking the Rho/Rho-kinase(ROCK) pathway can promote neurite outgrowth in spinal cord injury models. In the present study, we investigated neurite outgrowth and neuronal differentiation in neural stem cells from the mouse subventricular zone after inhibition of ROCK in vitro. Inhibition of ROCK with Y-27632 increased neurite length, enhanced neuronal differentiation, and upregulated the expression of two major signaling pathway effectors, phospho-Akt and phospho-mitogen-activated protein kinase, and the Hippo pathway effector YAP. These results suggest that inhibition of ROCK mediates neurite outgrowth in neural stem cells by activating the Hippo signaling pathway. | Xu-feng Jia Fei Ye Yan-bo Wang Da-xiong Feng | 2016 | Neural Regeneration Research2016，11，6： | 2 |
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