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| 1 | Ligand density-dependent influence of arginine-glycine- aspartate functionalized gold nanoparticles on osteogenic and adipogenic differentiation of mesenchymal stem cells显示文摘细胞外的矩阵(ECM ) 在调整房间功能和命运起一个很重要的作用。制作 biomimetic 模型在干细胞区别调查 ECM 的角色是高度合乎需要的。在这研究, arginine-glycine-aspartate (RGD ) 与悦耳的表面 ligand 修改了金 nanoparticles (Au NP ) 密度被准备模仿 ECM 微型环境。他们 osteogenic 和人的间充质的干细胞(MSC ) 的 adipogenic 区别上的效果被调查。biomimetic Au NP 被 MSC 以一种 ligand 密度依赖者方式收起。有高 RGD 密度的 biomimetic NP 在碱的磷酸酶(高山) 上有禁止的效果活动,钙免职,和 MSC 的 osteogenic 标记基因表示。当 RGD 密度是低的时,当 RGD 密度高时,他们油微滴形成和 adipogenic 标记基因表示上的效果是否定的,当他们的效果是提升的时。biomimetic Au NP 主要通过影响焦点的粘附和细胞骨架调整了 MSC 的 osteogenic 和 adipogenic 区别。这研究在能在为织物工程和生物医学的应用制作功能的 biomaterials 提供有意义的策略的干细胞区别上加亮 biomimetic NP 的角色。 | Jingchao Li Ying Chen Naoki Kawazoe Guoping Chen | 2018 | Nano Research2018，11，3： | 4 |
| 2 | Fluorescent silicon nanoparticles-based nanotheranostic agents for rapid diagnosis and treatment of bacteria-induced keratitis显示文摘Recommended as a medical emergency,infectious keratitis with an acute and rapid disease progression can lead to serious damage of vision and even blindness.Herein,we present a kind of theranostic agents,which are made of vancomycin(Van)-modified fluorescent silicon nanoparticles(SiNPs-Van),enabling rapid and non-invasive diagnosis and treatment of Gram-positive bacteria-induced keratitis in a simultaneous manner.Typically,the resultant SiNPs-Van nanoagents have an ability of imaging bacteria in a short time both in vitro(5 min)and in vivo(10 min),making them an efficacious diagnostic agent for the detection of bacterial keratitis.In addition,the SiNPs-Van feature distinct antimicrobial activity,with superior activity of 92.5%at a concentration of 0.5 ng/mL against Staphylococcus aureus(S.aureus);comparatively,the antimicrobial rate of free vancomycin is 23.3%at the same concentration.We further explore the SiNPs-Van agents as eye drops for therapy of S.aureus-induced bacterial keratitis on rat model.Represented by slit-lamp scores,the keratitis severity of SiNPs-Van-treated corneas is 3.4,which is 59.6%and 77.3%slighter than vancomycin-(8.2 score)and PBS-treated corneas(15.0 score),respectively.The infected corneas recover to normal(1 score)after 7-d of SiNPs-Van treatment.Above results suggest that the SiNPs-Van could serve as a new kind of high-quality nanotheranostic agents,especially suitable for simultaneous diagnosis and therapy of Gram-positive bacteria keratitis. | Lu Zhang Xiaoyuan Ji Yuanyuan Su Xia Zhai Hua Xu Bin Song Airui Jiang Daoxia Guo Yao He | 2021 | Nano Research2021，14，1： | 2 |
| 3 | Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment显示文摘Arginine-glycine-aspartic 酸(RGD )基于 dendrimer 的 nanopatterns 在上(L 乳的酸) poly 被用作 bioactive 底层在成年人的间充质的干细胞的 chondrogenic 正式就职上评估 RGD 本地表面密度的影响。在 chondrogenic 承诺期间,改变的活跃细胞外的矩阵(ECM ) 发生,起在区别过程的一个有启发性的作用。尽管象小团或 micromass 文化那样的三维的环境通常被使用为在 vitro chondrogenic 区别,这些文化相当关于他们在 cell-ECM 相互作用讯问房间的能力被限制。在现在的学习,悦耳的 RGD 表面密度的 nanopatterns 作为起始的 dendrimer 集中的功能被获得。本地 RGD 表面密度为最小的 interparticle 距离通过概率轮廓情节被确定,从相应原子力量显微镜学图象构造了,并且与房间粘附和区别反应相关。结果表明在 nanoscale 的本地 RGD 表面密度充当 chondrogenic 承诺的一个管理者,并且到底层的房间的那中间的粘着性赞成间充质的房间冷凝作用和早 chondrogenic 区别。 | Anna Lagunas Iro Tsintzou Yolanda Vida Daniel Collado Ezequiel Perez-Inestrosa Cristina Rodriguez Pereira Joana Magalhaes Jose A. Andrades Josep Samitier | 2017 | Nano Research2017，10，6： | 1 |
| 4 | Critical adhesion areas of cells on micro-nanopatterns显示文摘Cell adhesion to extracellular matrices(ECM)is critical to physiological and pathological processes as well as biomedical and biotechnological applications.It has been known that a cell can adhere on an adhesive microisland only over a critical size.But no publication has concerned critical adhesion areas of cells on microislands with nanoarray decoration.Herein,we fabricated a series of micro-nanopatterns with different microisland sizes and arginine-glycine-aspartate(RGD)nanospacings on a nonfouling poly(ethylene glycol)background.Besides reproducing that nanospacing of RGD,a ligand of its receptor integrin(a membrane protein),significantly influences specific cell adhesion on bioactive nanoarrays,we confirmed that the concept of critical adhesion area originally suggested in studies of cells on micropatterns was justified also on the micro-nanopatterns,yet the latter exhibited more characteristic behaviors of cell adhesion.We found increased critical adhesion areas of human mesenchymal stem cells(hMSCs)on nanoarrayed microislands with increased RGD nanospacings.However,the numbers of nanodots with respect to the critical adhesion areas were not a constant.A unified interpretation was then put forward after combining nonspecific background adhesion and specific cell adhesion.We further carried out the asymptotic analysis of a series of micro-nanopatterned surfaces to obtain the effective RGD nanospacing on unpatterned free surfaces with densely grafted RGD,which could be estimated nonzero but has never been revealed previously without the assistance of the micro-nanopatterning techniques and the corresponding analysis. | Shuang Zheng Qiong Liu Junhao He Xinlei Wang Kai Ye Xuan Wang Ce Yan Peng Liu Jiandong Ding | 2022 | Nano Research2022，15，2： | 0 |
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