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| 1 | 铁皮石斛DoCDPK基因的克隆与表达分析显示文摘利用RACE技术和RT-PCR相结合,从铁皮石斛(Dendrobium officinale Kimura et Migo)中克隆得到长度分别为1 863和1 729 bp的钙依赖蛋白激酶(calcium-dependent protein kinase)基因DoCDPK1与DoCDPK2的cDNA全长序列,开放阅读框分别为1 539和1 536 bp,编码512和511个氨基酸,编码蛋白表现为亲水性,结构稳定,二级结构主要由α–螺旋和无规卷曲构成。qRT-PCR分析结果表明DoCDPK1和DoCDPK2主要在铁皮石斛叶片中表达;4℃低温和400 mmol·L^(-1) NaCl处理后,DoCDPK1和DoCDPK2在各个时间点上(2、4、8、12和24 h)的表达量有不同程度提高,而100 mmol·L^(-1) ABA处理后DoCDPK1和DoCDPK2的表达量呈现不同变化趋势,推测DoCDPK1和DoCDPK2参与低温等非生物胁迫的响应。 | 盛况 高燕会 斯金平 朱玉球 | 2016 | 园艺学报2016，43，12： | 10 |
| 2 | Kiwifruit(Actinidia chinensis)R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana显示文摘Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses,such as drought,heat,cold,water logging and phytopathogens.Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement.Here we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog(AcMYB3R)whose expression was induced by drought,salinity and cold stress.In vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene KNOLLE.The Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt stress.The expressions of stress-responsive genes such as RD29A,RD29B,COR15A and RD22 were prominently up-regulated by ectopic expression of Ac MYB3R.Our study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production. | ZHANG Ya-bin TANG Wei WANG Li-huan HU Ya-wen LIU Xian-wen LIU Yong-sheng | 2019 | Journal of Integrative Agriculture2019，18，2： | 7 |
| 3 | 拟南芥AtTR1对AtCPK28和AtCPK32的体外泛素化修饰研究显示文摘为了研究E3连接酶AtTR1和非生物胁迫应答的相关蛋白AtCPK28、AtCPK32之间关系,本文构建原核表达载体pET28a-AtCPK28和pET28a-AtCPK32,并在大肠杆菌中表达了AtTR1、AtCPK28和AtCPK32重组蛋白,利用亲和层析纯化相应融合蛋白.将AtCPK28和AtCPK32蛋白分别加入含有ATP、泛素、E1、E2和AtTR1的体外泛素反应体系中,western blot检测到AtCPK28和AtCPK32有多聚泛素化条带,说明AtTR1在体外能多泛素化修饰AtCPK28和AtCPK32.结果表明AtCPK28和AtCPK32可能是AtTR1调控植物抗逆信号中的下游靶蛋白. | 樊莉娟 陈彩丽 朱旭辉 黄奎 姚润东 王健美 | 2017 | 四川大学学报(自然科学版)2017，54，3： | 2 |
| 4 | 青花菜钙依赖蛋白激酶基因BoCDPK1的克隆与表达显示文摘钙依赖蛋白激酶(calcium-dependent protein kinase,CDPK)为Ca^2+传感蛋白,在植物生长发育和逆境响应中起着重要作用。在克隆青花菜BoCDPK1基因的基础上,开展序列分析、系统发育分析和表达分析,为后续的基因功能鉴定和抗逆育种奠定基础。该研究以青花菜为材料,利用PCR法克隆1个CDPK基因,利用生物信息学对序列进行分析,并采用qRT-PCR研究该基因在霜霉菌和核盘菌侵染下的表达模式。测序结果表明,BoCDPK1的基因组DNA全长为2 414 bp,具6个内含子,编码区全长为1 647 bp,编码548个氨基酸;BoCDPK1有1个STKc和4个EF手性结构域。多序列比对结果表明,BoCDPK1与芸薹属植物同源序列的相似性最高,仅个别氨基酸残基存在差异,它们在系统发育树上聚于一组。qRT-PCR结果表明,BoCDPK1的表达受霜霉菌和核盘菌的诱导,表达量均呈现先上升后下降的规律。在霜霉菌的诱导下,BoCDPK1的表达量在72 h达最大值,为对照的3.4倍;而在核盘菌侵染下,BoCDPK1的表达量在36 h达最大值。该研究明确了青花菜BoCDPK1基因的序列特点、系统发育关系和表达特征。 | 邬菲帆 王佳怡 戴晨宇 杨如棉 徐鹏杰 管铭 张慧娟 蒋明 | 2020 | 浙江农业学报2020，32，4： | 2 |
| 5 | From Chinese Science Bulletin to Science Bulletin: celebrate the coming 50th birthday显示文摘As the only multidisciplinary journal supervised by Chinese Academy of Sciences and the National Natural Science Foundation of China,Science Bulletin will usher in its50th anniversary in 2016.A fifty-year history for a journal is always remarkable.However,the more important reason to celebrate besides the great age is:the fifty-year old journal continues to be vigorous and influential. | Xuming Jia Rui An Xiao-Ya Chen | 2015 | Science Bulletin2015，60，24： | 0 |
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