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| 1 | 羊肚菌菌塘土壤细菌群落的结构及多样性显示文摘为了解野生羊肚菌的生境,研究其菌塘土壤细菌群落的结构与多样性,运用PCR–DGGE技术,对采集于凉山州冕宁县野生羊肚菌发生地的8个羊肚菌菌塘土样和2个非菌塘土样进行分析。结果表明:羊肚菌菌塘土壤与非菌塘土壤细菌群落的结构差异和多样性差异较大;菌塘土壤的多样性指数(H)和丰度(S)比非菌塘土壤的高;菌塘土壤中Thermosporotrichaceae、Myxococcaceae和Rhodospirillaceae等3科细菌在羊肚菌菌塘土壤中占据优势地位;羊肚菌菌塘土壤细菌多样性程度总体优于非菌塘土壤。 | 熊川 李小林 李强 郑林用 | 2015 | 湖南农业大学学报(自然科学版)2015,41,4: | 17 |
| 2 | 四川松茸内生细菌群落结构与多样性显示文摘为了解松茸的内生细菌群落结构与多样性,以采自四川7个松茸主产区的14个松茸样品为材料,通过变性梯度凝胶电泳(DGGE)分析其内生细菌的群落结构与多样性差异,结果表明:各个产区松茸样品的内生细菌群落结构与多样性存在明显差异,但采自相近地点、相似环境的松茸样品内生细菌群落结构相似性较高;样品的丰度范围为15~25,多样性指数依次为盐源〉冕宁〉会东〉木里〉雅江〉盐边〉小金;条带回收测序构建的系统发育树显示,松茸内生细菌种群丰富,各个产区松茸优势种群存在一定的差异,但假单胞菌属、爱文菌属、芽孢杆菌属在所有样品中均有分布,而且都占据一定的优势地位,产碱杆菌属与鞘氨醇杆菌属在大部分样品中为优势种群;而杜擀氏菌属、赖氨酸芽孢杆菌属在特定样品中为优势种群,表明松茸内生细菌具有丰富的多样性,研究结果可为筛选促进松茸生长的内生菌提供依据。 | 李强 李小林 黄文丽 熊川 杨阳 杨志荣 郑林用 | 2014 | 应用生态学报2014,25,11: | 7 |
| 3 | 响应面法优化石油降解菌性能的研究显示文摘从延长油田石油污染土壤中筛选出4株能以原油为唯一碳源生长的细菌单菌株,鉴定其优势菌株YC-2为枯草芽孢杆菌;利用响应面法进行实验设计,选定培养温度、p H值、氮磷比以及盐度为影响因素,对原油含量为0.5%的培养基进行YC-2菌株降解工艺优化,得到了原油降解率与4种因素之间的非线性回归方程,确定了降解原油的最优工艺条件:培养温度28℃;p H值6.5,氮磷比5.2∶1,盐度0.45%。在最优条件下,预测降解率为53.9%,实验验证值为52.5%,结果显示,建立的模型具有较高的精度。 | 牛志睿 山宝琴 刘羽 李卓珏 赵姣姣 庞康 张咪 | 2016 | 环境工程学报2016,10,3: | 5 |
| 4 | Effect of petroleum on decomposition of shrub–grass litters in soil in Northern Shaanxi of China显示文摘The impacts of petroleum contamination on the litter decomposition of shrub–grass land would directly influence nutrient cycling, and the stability and function of ecosystem. Ten common shrub and grass species from Yujiaping oil deposits were studied. Litters from these species were placed into litterbags and buried in petroleum-contaminated soil with 3levels of contamination(slight, moderate and serious pollution with petroleum concentrations of 15, 30 and 45 g/kg, respectively). A decomposition experiment was then conducted in the lab to investigate the impacts of petroleum contamination on litter decomposition rates. Slight pollution did not inhibit the decomposition of any litters and significantly promoted the litter decomposition of Hippophae rhamnoides, Caragana korshinskii, Amorpha fruticosa, Ziziphus jujuba var. spinosa, Periploca sepium, Medicago sativa and Bothriochloa ischaemum. Moderate pollution significantly inhibited litter decomposition of M. sativa,Coronilla varia, Artemisia vestita and Trrifolium repens and significantly promoted the litter decomposition of C. korshinskii, Z. jujuba var. spinosa and P. sepium. Serious pollution significantly inhibited the litter decomposition of H. rhamnoides, A. fruticosa, B. ischaemum and A. vestita and significantly promoted the litter decomposition of Z. jujuba var. spinosa, P.sepium and M. sativa. In addition, the impacts of petroleum contamination did not exhibit a uniform increase or decrease as petroleum concentration increased. Inhibitory effects of petroleum on litter decomposition may hinder the substance cycling and result in the degradation of plant communities in contaminated areas. | Xiaoxi Zhang Zengwen Liu Qi Yu Nhu Trung Luc Yuanhao Bing Bochao Zhu Wenxuan Wang | 2015 | Journal of Environmental Sciences2015,27,7: | 3 |