共被期刊论文引用了3次
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| 1 | 植物内生菌多样性及其病害生防机制研究进展显示文摘综述已报道的单子叶植物与双子叶植物内生菌的组成、寄主、不同部位分布特点及组织偏好性,讨论植物内生菌通过抗菌、营养竞争和诱导寄主免疫反应等抑制植物病原菌阻止病害发生的主要作用机制,针对微生物之间互作对寄主植物的影响、寄主植物在不同生长条件和不同发育时期对微生物种群结构的调控机制以及在农作物育种中构建适应性更强的功能共生体等提出展望。 | 巫艳 周云莹 朱玺燊 张力敏 SAUBANMUSA Jibril 杨志兵 王一 李成云 | 2022 | 云南农业大学学报(自然科学版)2022，37，5： | 6 |
| 2 | 降解纤维素产甲烷的四菌复合系显示文摘自然环境中通常是微生物群落协同完成纤维素的降解,构建可降解纤维素的混菌体系是认识微生物相互作用的关键.利用富集培养法,结合变性梯度凝胶电泳(DGGE)指纹检测技术以及厌氧滚管技术,建立了一种筛选简单降解纤维素产甲烷复合菌系的方法.利用此方法从青藏高原若尔盖高寒湿地分离到一个由4株菌构成的降解纤维素产甲烷的稳定菌系.结果表明,该复合菌系由纤维素水解菌Clostridium glycolicum、非纤维素水解菌Trichococcus flocculiformis和Parabacteroides merdae、产甲烷古菌Methanobacterium subterraneum等具有不同功能的4种菌株组成,且在这4株菌的共同作用下可将纤维素直接转化为CH4.该简单复合系的获得为今后纤维素转化甲烷复合菌系的代谢控制和遗传改造提供了一个平台. | 吴俊妹 马安周 崔萌萌 于清 齐鸿雁 庄绪亮 庄国强 | 2014 | 环境科学2014，35，1： | 6 |
| 3 | Construction of a dual fluorescence whole-cell biosensor to detect N-acyl homoserine lactones显示文摘Detection of N-acyl homoserine lactones(AHLs) is useful for understanding quorum sensing(QS) behaviors, including biofilm formation, virulence and metabolism. For detecting AHLs and indicating the host cells in situ, we constructed the plasmid pUCGMA2T1-4 to make a dual fluorescent wholecell biosensor based on the AhlI/R AHL system of Pseudomonas syringae pv. syringae B728a. The plasmid contains three components: constitutively expressed PnptII::gfp for indicating host cells, PahlI::mcherry that produces red fluorescence in response to AHL, and the ahlR gene that encodes an AHL regulatory protein. Meanwhile, two copies of T1-4(four tandem copies of a transcriptional terminator) were added into the plasmid to reduce background. The results showed that when the plasmid was placed into Escherichia coli, the dual fluorescence whole-cell biosensor was able to respond with red fluorescence within 6 hr to 5 × 10-8–1 × 10-5mol/L of 3OC6-HSL. Bright green fluorescence indicated the host cells. Furthermore, when the plasmid was transferred to wildtype Pseudomonas PhTA125(an AHL-producing bacterium), it also showed both green and red fluorescence. This result demonstrates that this plasmid can be used to construct whole-cell indicators that can indicate the AHL response and spatial behaviors of microbes in a microenvironmental niche. | Xuemei Deng Guoqiang Zhuang Anzhou Ma Qing Yu Xuliang Zhuang | 2014 | Journal of Environmental Sciences2014，26，2： | 1 |
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