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| 1 | GS6, A Member of the GRAS Gene Family,Negatively Regulates Grain Size in Rice显示文摘Grain size is an important yield related trait in rice.Intensive arti cial selection for grain size during domestication is evidenced by the larger grains of most of today’s cultivars compared with their wild relatives.However,the molecular genetic control of rice grain size is still not well characterized.Here,we report the identi cation and cloning of Grain Size 6(GS6),which plays an important role in reducing grain size in rice.A premature stop at the t348 position in the coding sequence(CDS)of GS6 increased grain width and weight signi cantly.Alignment of the CDS regions of GS6 in 90 rice materials revealed three GS6alleles.Most japonica varieties(95%)harbor the Type I haplotype,and 62.9%of indica varieties harbor the Type II haplotype.Association analysis revealed that the Type I haplotype tends to increase the width and weight of grains more than either of the Type II or Type III haplotypes.Further investigation of genetic diversity and the evolutionary mechanisms of GS6showed that the GS6gene was strongly selected in japonica cultivars.In addition,a'ggc'repeat region identi ed in the region that encodes the GRAS domain of GS6 played an important historic role in the domestication of grain size in rice.Knowledge of the function of GS6might aid efforts to elucidate the molecular mechanisms that control grain development and evolution in rice plants,and could facilitate the genetic improvement of rice yield. | Lianjun Sun Xiaojiao Li Yongcai Fu Zuofeng Zhu Lubin Tan Fengxia Liu Xianyou Sun Xuewen Sun Chuanqing Sun | 2013 | Journal of Integrative Plant Biology2013，55，10： | 44 |
| 2 | Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System显示文摘 | Lu, Yuming Ye, Xiao Guo, Renming Huang, Jing Wang, Wei Tang, Jiuyou Tan, Longtao Zhu, Jian-kang Chu, Chengcai Qian, Yangwen | 2017 | Molecular Plant2017，10，9： | 28 |
| 3 | Response of Rice to Insect Elicitors and the Role of OsJAR1 in Wound and Herbivory-Induced JA-lle Accumulation显示文摘Plants produce jasmonic acid(JA) and its amino acid conjugate,jasmonoyl-L-isoleucine(JA-lle) as major defense signals in response to wounding and herbivory.In rice(Oryza sativa),JA and JA-lle rapidly increased after mechanical damage,and this increase was further amplified when the wounds were treated with oral secretions from generalist herbivore larvae,lawn armyworms(Spodoptera mauritia),revealing for the first time active perception mechanisms of herbivore-associated elicitor(s) in rice.In the rice genome, two OsJAR genes can conjugate JA and lle and form JA-lle in vitro;however,their function in herbivoryinduced accumulation of JA-lle has not been investigated.By functional characterization of TOS17 retrotransposon-tagged Osjar1 plants and their response to simulated herbivory,we show that OsJAR1 is essential for JA-lle production in herbivore-attacked,field-grown plants.In addition,OsJAFM was required for normal seed development in rice under field conditions.Our results suggest that OsJAR1 possesses at least two major functions in rice defense and development that cannot be complemented by the additional OsJAR2 gene function,although this gene previously showed overlapping enzyme activity in vitro. | Kaori Fukumoto Kabir Md Alamgir Yuko Yamashita Izumi C.Mori Hideyuki Matsuura Ivan Galis | 2013 | Journal of Integrative Plant Biology2013，55，8： | 5 |
| 4 | 甘蓝型油菜花序长发夹lhRNAi文库的构建显示文摘异源四倍体油菜含有大量多拷贝基因及冗余基因,难以通过T-DNA插入、物理及化学诱变等常规突变体库创制方法发掘功能基因。为开发适应于油菜简单高效的突变体库创制方法,本研究通过滚环复制方法构建了甘蓝型油菜的花序lhRNAi文库,并对这种新型的干扰文库进行质量评估;然后将该花序lhRNAi文库对甘蓝型油菜进行遗传转化,获得763株T0植株,从中鉴定分离出74株具有可见表型变异的花发育相关突变体,包括花瓣减少、形状异常,柱头卷曲,雄蕊退化萎缩,雄性不育、死蕾或花蕾闭合等。说明通过滚环复制介导的lhRNAi文库的构建方法可以成功应用到油菜中,这将为油菜花序发育相关基因的功能研究提供重要的研究平台。 | 曾新华 赵圣博 李可琪 袁荣 吴刚 闫晓红 | 2019 | 中国油料作物学报2019，41，3： | 0 |
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