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| 1 | Cold-Induced CBF–PIF3 Interaction Enhances Freezing Tolerance by Stabilizing the phyB Thermosensor in Arabidopsis显示文摘Growth inhibition and cold-acclimation strategies help plants withstand cold stress,which adversely affects growth and survival.PHYTOCHROME B(phyB)regulates plant growth through perceiving both light and ambient temperature signals.However,the mechanism by which phyB mediates the plant response to cold stress remains elusive.Here,we show that the key transcription factors mediating cold acclimation,C-REPEAT BINDING FACTORs(CBFs),interact with PHYTOCHROME-INTERACTING FACTOR 3(PIF3)under cold stress,thus attenuating the mutually assured destruction of PIF3–phyB.Cold-stabilized phyB acts downstream of CBFs to positively regulate freezing tolerance by modulating the expression of stress-responsive and growth-related genes.Consistent with this,phyB mutants exhibited a freezing-sensitive phenotype,whereas phyB-overexpression transgenic plants displayed enhanced freezing tolerance.Further analysis showed that the PIF1,PIF4,and PIF5 proteins,all of which negatively regulate plant freezing tolerance,were destabilized by cold stress in a phytochrome-dependent manner.Collectively,our study reveals that CBFs–PIF3–phyB serves as an important regulatory module for modulating plant response to cold stress. | Bochen Jiang Yiting Shi Yue Peng Yuxin Jia Yan Yan Xiaojing Dong Hui Li Jie Dong Jigang Li Zhizhong Gong Michael F.Thomashow Shuhua Yang | 2020 | Molecular Plant2020，13，6： | 13 |
| 2 | The G-Protein β Subunit AGB1 Promotes Hypocotyl Elongation through Inhibiting Transcription Activation Function of BBX21 in Arabidopsis显示文摘 | | 2017 | Molecular Plant2017，10，9： | 7 |
| 3 | 植物BBX转录因子基因家族的研究进展显示文摘转录因子在调控植物生长、发育及环境适应性等方面发挥重要作用。具有B-box结构域的一类锌指结构转录因子称为BBX,它们通过调控基因转录,与同类或其他转录因子的互作参与植物光形态建成、花发育、避荫效应、植物信号转导以及非生物和生物逆境响应等。文中从BBX蛋白结构、分类以及其功能方面对该类转录因子在植物中的作用进行了综述。 | 杨宁 从青 程龙军 | 2020 | 生物工程学报2020，36，4： | 6 |
| 4 | 小麦B-box基因家族全基因组鉴定与表达分析显示文摘B-box(BBX)是一类含有1个或2个B-box结构域的锌指蛋白,在植物生长发育中起着重要作用。本研究明确小麦B-box转录因子的数量、基因结构和分类进化关系,研究各基因成员在不同组织中的特异性表达以及对非生物胁迫的响应。从小麦全基因组中鉴定得到87个B-box基因家族成员,所有TaBBXs蛋白均含有B-box结构域。TaBBXs编码146~489个氨基酸,理论等电点为4.32~10.42。染色体定位分析表明,TaBBXs分布在除1A、1B和1D之外的18条小麦染色体上。通过系统发育分析将TaBBXs划分为5个亚家族,有0~4个内含子。在同组内同一个系统进化树分支中的亚族成员具有高度相似的基因结构。qRT-PCR分析的20个TaBBXs基因,具有不同的组织表达模式,16个基因在叶中有较高表达,TaBBX10和TaBBX39仅在叶中有较高表达,而TaBBX74在穗中表达,TaBBX43在根中特异性表达。在不同逆境胁迫下,TaBBXs呈现不同表达模式,11个基因在低温胁迫后上调表达,12个基因在ABA处理后下调表达,盐胁迫后10个基因出现上调表达,干旱胁迫后7个基因出现下调表达,TaBBX10、TaBBX39、TaBBX60、TaBBX67和TaBBX74基因在2种或2种以上胁迫下有显著的上调表达。 | 王艳朋 凌磊 张文睿 王丹 郭长虹 | 2021 | 作物学报2021，47，8： | 6 |
| 5 | B-box proteins:Pivotal players in light-mediated development in plants^FA显示文摘Light signals mediate a number of physio-logical and developmental processes in plants, such as flowering, photomorphogenesis, and pigment accu-mulation. Emerging evidence has revealed that a group of B-box proteins (BBXs) function as central players in these light-mediated developmental processes. B-box proteins are a class of zinc-coordinated transcription factors or regulators that not only directly mediate the transcription of target genes but also interact with various other factors to create a complex regulatory network involved in the precise control of plant growth and development. This review summarizes and highlights the recent findings concerning the critical regulatory functions of BBXs in photoperiodic flow-ering, light signal transduction and light-induced pigment accumulation and their molecular modes of action at the transcriptional and post-translational levels in plants. | Zhaoqing Song Yeting Bian Jiujie Liu Yuting Sun Dongqing Xu | 2020 | Journal of Integrative Plant Biology2020，62，9： | 5 |
| 6 | Phytochromes and Phytohormones: The Shrinking Degree of Separation显示文摘 | Qin wang Ziqiang Zhu Kara Ozkardesh Chentao Lin | 2013 | Molecular Plant2013，6，1： | 1 |
| 7 | BBX11 promotes red light-mediated photomorphogenic development by modulating phyB-PIF4 signaling显示文摘phytochrome B(phyB)acts as the red light photoreceptor and negatively regulates the growth-promoting factor PHYTOCHROME INTERACTING 4(PIF4)through a direct physical interaction,which in turn changes the expression of a large number of genes.phyB-PIF4 module regulates a variety of biological and developmental processes in plants.In this study,we demonstrate that B-BOX PROTEIN 11(BBX11)physically interacts with both phyB and PIF4.BBX11 negatively regulates PIF4 accumulation as well as its biochemical activity,consequently leading to the repression of PIF4-controlled genes’expression and promotion of photomorphogenesis in the prolonged red light.This study reveals a regulatory mechanism that mediates red light signal transduction and sheds a light on phyB-PIF4 module in promoting red light-dependent photomorphognenesis. | Zhaoqing Song Yueqin Heng Yeting Bian Yuntao Xiao Jiujie Liu Xianhai Zhao Yan Jiang Xing Wang Deng Dongqing Xu | 2021 | aBIOTECH2021，2，2： | 1 |
| 8 | 水稻B-box锌指蛋白基因OsBBX26的克隆及表达分析显示文摘【目的】克隆水稻B-box锌指蛋白基因(OsBBX26),并分析其在非生物胁迫下的表达模式,为探究水稻B-box蛋白的非生物胁迫响应机制提供理论依据。【方法】以水稻模式品种日本晴为材料,采用RT-PCR克隆OsBBX26基因,对其进行生物信息学分析,并通过构建pBA-OsBBX26-YFP植物表达载体进行亚细胞定位。同时,采用实时荧光定量PCR检测分析OsBBX26基因的组织特异性及非生物胁迫(干旱、ABA、低温和高盐)下的表达模式。【结果】克隆获得的OsBBX26基因(LOC_Os08g42440)cDNA全长序列为1467 bp,编码488个氨基酸,其编码蛋白分子量为51.86 kD,理论等电点(pI)为6.63,包含1个B-box锌指结构域和1个CCT结构域,其中B-box结构域位于第13~60位氨基酸,CCT结构域位于第435~478位氨基酸,该蛋白定位于细胞核中。OsBBX26基因位于8号染色体上,启动子区域除了含有TATA-Box和CAAT-Box等基本转录元件外,还含有与逆境相关的顺式作用元件,包括低温响应元件LTR、脱落酸响应元件ABRE、水杨酸响应元件TCA-element、厌氧响应元件ARE、茉莉酸甲酯响应元件CGTCA-motif和TGACG-motif,以及MYB转录因子结合位点。OsBBX26蛋白与粳稻B-box蛋白亲缘关系最近,其次与籼稻B-box蛋白亲缘关系较近,与其他植物B-box蛋白也存在较高的相似性,其中与小米、大麦、高粱、玉米和短柄草B-box蛋白的氨基酸序列相似性分别达72%、72%、71%、69%和68%。OsBBX26基因在幼叶中的表达量最高,其次是在成熟叶片中,在幼根、成熟根、茎和幼穗中的表达量均极低,表明该基因表达具有明显的组织表达特异性。干旱、高盐、ABA和低温胁迫处理下OsBBX26基因均上调表达,干旱、高盐和ABA胁迫处理12 h达峰值,但低温胁迫处理在24 h后才达峰值。【结论】OsBBX26基因受干旱、高盐、ABA和低温胁迫诱导均上调表达,推测其参与水稻植株响应多种非生物胁迫,在逆境响应中发挥重要调控作用。 | 邓军 罗正良 朱菲莹 陈希 方勇 任鄄胜 任光俊 高方远 | 2020 | 南方农业学报2020，51，7： | 1 |
| 9 | Photomorphogenesis——from One Photoreceptor to 14:40 Years of Progress显示文摘 | Winslow R. Briggs Chen-Tao Lin | 2012 | Molecular Plant2012，5，3： | 1 |
| 10 | SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato显示文摘Anthocyanins play vital roles in plant stress tolerance and growth regulation.Previously,we reported that the photomorphogenesis-related transcription factor SlBBX20 regulates anthocyanin accumulation in tomato.However,the underlying mechanism remains unclear.Here,we showed that SlBBX20 promotes anthocyanin biosynthesis by binding the promoter of the anthocyanin biosynthesis gene SlDFR,suggesting that SlBBX20 directly activates anthocyanin biosynthesis genes.Furthermore,we found by yeast two-hybrid screening that SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2,and the interaction was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays.SlCSN5 gene silencing led to anthocyanin hyperaccumulation in the transgenic tomato calli and shoots,and SlCSN5-2 overexpression decreased anthocyanin accumulation,suggesting thSlCSN5-2 enhanced the ubiquitination of SlBBX20 and promoted the degradation of SlBBX20 in vivo.Consistently,silencing the SlCSN5-2 homolog in tobacco significantly increased the accumulation of the SlBBX20 protein.Since SlBBX20 is a vital regulator of photomorphogenesis,the SlBBX20-SlCSN5-2 module may represent a novel regulatory pathway in light-induced anthocyanin biosynthesis. | Dan Luo Cheng Xiong Aihua Lin Chunli Zhang Wenhui Sun Junhong Zhang Changxian Yang Yongen Lu Hanxia Li Zhibiao Ye Ping He Taotao Wang | 2021 | Horticulture Research2021，8，1： | 0 |
| 11 | 潘那利番茄转录因子SpBBX1基因的克隆、表达及耐逆性初步分析显示文摘B-box(BBX)转录因子在幼苗的光形态建成、开花的光周期调节、避光等生长发育过程以及对生物和非生物胁迫的响应中发挥着重要作用。本研究从野生潘那利番茄(Solanum pennellii)中获得了1个BBX蛋白基因,命名为SpBBX1(基因ID:Sopen02g034260),对其进行了理化性质、蛋白结构、进化树分析,并观察了该转录因子的亚细胞定位,分析了原核表达及胁迫耐受性。结果显示,该基因包含1个1173 bp的完整开放阅读框,编码390个氨基酸,定位于细胞核;进化树显示其氨基酸序列与栽培番茄(S.lycopersicum)开花期基因SlCONSTANS1(基因ID:Solyc02g089540)及马铃薯(S.tuberosum)BBX蛋白1(StB-box1)(基因ID:PGSC0003DMP400017796)亲缘关系最近。SDS-PAGE电泳及Western blot结果显示,SpBBX1重组蛋白的分子量约为48 kD。qRT-PCR分析发现,SpBBX1在茎、叶、花中均有表达,叶片中的表达量最高。原核重组大肠杆菌(Escherichia coli)BL21::pET-30a-SpBBX1在NaCl和甘露醇胁迫下生长显著低于对照菌(P<0.05)。本研究结果揭示了潘那利番茄SpBBX1基因可能在盐胁迫响应中具有负调控效应,为进一步研究植物BBX蛋白的功能提供理论依据。 | 赵四方 周涛 胡佳惠 兰海燕 余庆辉 | 2021 | 农业生物技术学报2021，29，9： | 0 |
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