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| 1 | 番茄疮痂病抗性遗传研究和基因定位最新进展显示文摘由黄单胞杆菌(Xanthomonas)引起的番茄疮痂病是严重影响番茄生产的一种细菌性病害。近年来,该病害病原菌小种的快速变化,特别是X.gardneri的扩散,加速了人们对抗性遗传、基因定位和分子标记辅助育种的研究工作。迄今已经定位了3个抗T3小种的基因、5个抗T3小种的QTL和3个抗T4小种的QTL,并对部分基因或QTL进行了精细定位,建立了标记辅助选择体系,育成了抗T4小种的品种。本文将就这些最新的研究进展进行总结,对现存问题进行分析,并对番茄疮痂病抗性遗传研究和育种应用前景进行探讨,以期为抗番茄疮痂病育种提供参考。 | 杨文才 | 2013 | 园艺学报2013，40，9： | 7 |
| 2 | Tomato SlPUB24 enhances resistance to Xanthomonas euvesicatoria pv. perforans race T3显示文摘Solanum lycopersicum var.cerasiforme accession PI 114490 has broad-spectrum resistance to bacterial spot caused by several species of Xanthomonas.Resistance is quantitatively inherited,and a common quantitative trait locus QTL-11B on chromosome 11 has been identified previously.In this study,the SlPub24 gene was characterized in QTL-11B.SlPub24 in PI 114490 was upregulated by infection with X.euvesicatoria pv.perforans race T3,but its transcription was low in the susceptible line OH 88119 whether or not it was infected by the pathogen.The differential expression of SlPub24 between PI 114490 and OH 88119 was due to great sequence variation in the promoter region.The promoter of SlPub24 in OH 88119 had very low activity and did not respond to pathogen infection.Transgenic lines of OH 88119 overexpressing SlPub24 isolated from PI 114490 showed significantly enhanced resistance,while mutants of Slpub24 generated by CRISPR/Cas9 editing showed more susceptibility to race T3 and to other races.The mutants also showed spontaneous cell death in leaves.The expression of the salicylic acid(SA)pathway gene phenylalanine ammonia-lyase(PAL)and signaling-related genes pathogenesis-related(PR1)and nonexpresser of PR1(NPR1)were influenced by SlPub24.The content of SA in tomato plants was consistent with the level of SlPub24 expression.Furthermore,SlPUB24 interacted with the cell wall protein SlCWP and could regulate the degradation of SlCWP.The expression levels of SlCWP and SlCWINV1,a cell wall invertase gene,showed opposite patterns during pathogen infection.The activity of SlCWINV1 was lower in mutants than in PI 114490.The results are discussed in terms of the roles of the abovementioned genes,and a potential model for SlPUB24-mediated resistance to bacterial spot is proposed. | Xin Liu Ge Meng Mengrui Wang Zilin Qian Yaxian Zhang Wencai Yang | 2021 | Horticulture Research2021，8，1： | 4 |
| 3 | High throughput sequencing unravels tomato-pathogen interactions towards a sustainable plant breeding显示文摘Tomato(Solanum lycopersicum)is one of the most economically important vegetables throughout the world.It is one of the best studied cultivated dicotyledonous plants,often used as a model system for plant research into classical genetics,cytogenetics,molecular genetics,and molecular biology.Tomato plants are affected by different pathogens such as viruses,viroids,fungi,oomycetes,bacteria,and nematodes,that reduce yield and affect product quality.The study of tomato as a plant-pathogen system helps to accelerate the discovery and understanding of the molecular mechanisms underlying disease resistance and offers the opportunity of improving the yield and quality of their edible products.The use of functional genomics has contributed to this purpose through both traditional and recently developed techniques,that allow the identification of plant key functional genes in susceptible and resistant responses,and the understanding of the molecular basis of compatible interactions during pathogen attack.Next-generation sequencing technologies(NGS),which produce massive quantities of sequencing data,have greatly accelerated research in biological sciences and offer great opportunities to better understand the molecular networks of plant–pathogen interactions.In this review,we summarize important research that used high-throughput RNA-seq technology to obtain transcriptome changes in tomato plants in response to a wide range of pathogens such as viruses,fungi,bacteria,oomycetes,and nematodes.These findings will facilitate genetic engineering efforts to incorporate new sources of resistance in tomato for protection against pathogens and are of major importance for sustainable plant-disease management,namely the ones relying on the plant’s innate immune mechanisms in view of plant breeding. | Maria Doroteia Campos Maria do Rosario Felix Mariana Patanita Patrick Materatski Carla Varanda | 2021 | Horticulture Research2021，8，1： | 1 |
| 4 | Allelic Tests and Sequence Analysis of Three Genes for Resistance to Xanthomonas perforans Race T3 in Tomato显示文摘Three crosses,Hawaii7981×PI128216,Hawaii7981×LA1589,and PI128216×LA1589,were made to develop F_2 populations for testing allelism among three genes Xv3,Rx4,and Rx_(LA1589) conferring resistance to bacterial spot caused by Xanthomonas perforans race T3 in tomato. Each population consisted of 535–1 655 individuals. An infiltration method was used to inoculate the leaves of the parental and F_2 plants as well as the susceptible control OH88119 for detecting hypersensitive resistance(HR). The results showed that all the tomato plants except OH88119 had HR to race T3,indicating that Xv3,Rx4,and Rx_(LA1589) were allelic genes. Genomic DNA fragments of the Rx4 alleles from Hawaii7981,PI128216,and LA1589 were amplified using gene-specific primers and sequenced. No sequence variation was observed in the coding region of Rx4 in the three resistant lines. Based on the published map positions of these loci as well as the allelic tests and sequence data obtained in this study,we speculated that Xv3,Rx4,and Rx_(LA1589) were the same gene. The results will provide useful information for understanding the mechanism of resistance to race T3 and developing resistant tomato varieties. | ZHAO Baimei CAO Haipeng DUAN Junjie YANG Wencai | 2015 | Horticultural Plant Journal2015，1，1： | 1 |
| 5 | 番茄疮痂病抗性分子标记及其在育种中的应用显示文摘为了加速抗番茄疮痂病番茄品种育种进程,综述了番茄疮痂病抗性分子标记及其在抗性材料选育中的应用现状,指出存在的问题,一是番茄疮痂病病原菌在同一地区存在多个种和小种并存的现象,难以实施采用多系品种防控由多个种或小种引起的病害的策略;二是番茄材料抗疮痂病基因或QTL往往与不良性状连锁,限制了抗性基因或QTL的应用。最后,提出筛选对番茄疮痂病具有广谱的高抗或免疫材料、加大番茄疮痂病抗性机理研究力度等建议,从而为抗病育种提供策略。 | 孟歌 杨璐瑶 杨文才 | 2021 | 蔬菜2021，，S01： | 0 |
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