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| 1 | Regulation of fertilization in male rats by CatSper2 knockdown显示文摘对为避孕的药目标与某些离子隧道子单元专门位于精子的实现成长了的离子隧道的兴趣。选择子单元能导致的离子隧道击倒这些离子隧道的没有有病的效果的不孕,和选择禁止者或启子能防碍精子功能。在这研究,在 vivo electroporation (EP ) 和网睾丸,调停 microinjection 的 plasmid DNA 被采用到沉默 CatSper2 表示,它在精子 hyperactivation 是必要的。结果证明高 transfection 效率和表示被直接被注入网睾丸的 plasmid DNA 完成。由于 CatSper2 正在被堵住的表达式,给看的处理组显著地降低(P< 0.05 ) hyperactivation 率,在 vitro 的授精率,在粘弹性的答案的移植活动性和细胞内部的 Ca 2+ 达到顶点。低 hyperactivation 和授精率持续了 60 天。同时,精子幸存率的分析和睾丸组织学显示在 vivo, EP 没在睾丸,精子发生或精子活动的功能上有重要效果。现在的学习证明由 silencing 完成男避孕是可行的 CatSper2 的表示,涉及精子 hyperactivation 的关键蛋白质。 | Zhen Zhang Gen-Lin Wang Hui-Xia Li Lian Li Qun-Wei Cui Cheng-Bin Wei Fei Zhou | 2012 | Asian Journal of Andrology2012，14，2： | 3 |
| 2 | Knockdown of sAC affects sperm hyperactivation by cAMP-signaling pathway in male rat (Rattus norvegicus)显示文摘Soluble adenylyl cyclase (sAC) plays a critical role in male fertility in mammals by regulating sperm hyperactivation. We aimed to study the mechanism of sAC in this phenomenon and to explore potential target sites for male contraception. In this study, in vivo electroporation and rete testis microinjection-mediated short hairpin (sh)RNA plasmids were adopted to silence sAC gene expression in male rats. The results showed that high transfection efficiency (shRNA717, 49.0% and shRNA4205, 65.0%) was achieved by shRNA plasmids injected directly into the rete testis. When the sAC was downregulated, the cyclic adenosine mono-phosphate (cAMP) content and protein phosphorylation level of spermatozoa both declined with a significantly lower hyperactivation rate compared with negative controls. The highest transfection efficiency occurred at 15 d and was obviously time dependent. Bioinformatic and experimental results showed that sAC and tmAC both belong to the AC family and might have analogous functions. ShRNA717 and shRNA4205 were the best targets for the sAC gene, suggesting that they could be candidates for male contraception. Thus, it appears feasible to achieve male contraception by silencing the expression of sAC, affecting sperm hyper-activation via a cAMP-mediated signaling pathway. | YU Jing ZHOU Shuai JIANG XiaoQiang BAI JingYan WANG GenLin | 2013 | Chinese Science Bulletin2013，58，26： | 0 |
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